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The map label for this gene is thsB [H]

Identifier: 21227481

GI number: 21227481

Start: 1656363

End: 1658018

Strand: Reverse

Name: thsB [H]

Synonym: MM_1379

Alternate gene names: 21227481

Gene position: 1658018-1656363 (Counterclockwise)

Preceding gene: 21227484

Following gene: 21227479

Centisome position: 40.48

GC content: 51.27

Gene sequence:

>1656_bases
ATGGCAGGACAGCCAATATTCATTTTAAAAGAAGGAAGTAAGAGAACAAGAGGCAGGGATGCCCAGAGCAACAACATTAT
GGCTGCAAAAGCAGTAGCAGAAGCAGTCAGGACCACCCTTGGTCCCAAAGGTATGGACAAGATGCTTGTGGACTCAATGG
GCGATGTGGTCATTACAAACGATGGAGCAACCATTCTCAAAGAGATGGACATCGAGCACCCTGCAGCAAAGATGGTGGTA
GAAGTATCCAAGACCCAGGACGATGAAGTAGGAGACGGGACCACCAGTGCAGCTGTTGTCGCAGGTGAACTCTTAAACAA
AGCCGAGGACCTGATCGAACAGGAAATTCACCCGACAATTATTGCATCCGGATACAGACTGGCAGCCGAGAAAGCTATTG
AGGTTCTTAACTCCCTGGCAATGAGTGTAGACATGGGCAACAGGGACCTTCTGCTCAGCATTGCCGAAACTGCCATGACC
GGAAAAGGTGCGGAGTCCTCCAAAAAGCTTCTTGCTGAAATTGCCGTGGATGCCGTTACAAGCGTTGTCGACACAAACGG
CAAGATGTCAGTTGACAAGGAAAACATCAGCGTTGTCAAGAAAGTCGGCGGCAAAACCGAAGATTCCGAGCTCATCCCAG
GCATGATCATTGACAAGGAAAGAGTCCACACAAATATGCCTGAGAAAGTAAAGGATGCAAAGATCGCCCTCCTGAACACC
GCAATCGAACTTAAAGACACCGAAGTGGACGCAGAAATCTCAATCACCTCTCCTGACCAGCTTCAGTCCTTCCTTGATCA
GGAAGAGCAGATGCTCAAGAAGATCGTCCAGAAAGTTATCAACAGCGGCGCAAATGTAGTATTCTGCCAGAAGGGTGTCG
AAGACCTCGCCCAGCACTACCTTGCAAAAGCAGGTATCTTCGCAGTCCGCAGAGTCAAGAAGAGCGATATGGAAAAACTC
GCAAGAGCAACTGGCGGCAAACTCATCACCAACCTTGACGAGATCGTCCCCGAAGACCTTGGCTTTGCCAAACTCGTTGA
AGAGAAGAAGGTTGGCGGCGACAGCATGACCTTTGTCACAGGCTGCGACAACCCGAAGGCTGTTACCATTCTTCTGCGCG
GTGGCACAGAACATGTGGTTGACAGCATCGACAGCGCTCTTGAAGACGCCCTCCGCGTAGTAGGAGTTGCAATCGAAGAC
GAAAAGCTCGTTGCAGGCGGCGGCTCACCTGAAGTTGAGGTTGCTCTCAGGCTACAGGAATACGCAGCAACCCTCGAAGG
CAGGGAACAGCTTGCAGTAAAAGCTTACTCCGAAGCTCTTGAAGTTATCCCCAGGACTCTTGCAGAGAACGCAGGTCTCG
ACCCGATAGACATGCTCATGGAGCTCCGCTCCCAGCACGAGAAAGGCATGAAGACTGCAGGTCTCAACGTTTACGAAGGC
AAGGTCGTTGACATGTGGGAGAACTTCGTAGTCGAACCCCTCAGAGTCAAGACCCAGGTAATCAATGCAGCCACAGAGTC
TGCAGTCATGATCCTCAGGATCGACGACATCATTGCTTCTACCCGTGCAGCAGGCCCAAGCCCTGAAGAAATGGGTGGAA
TGCCGGGAATGGGTGGAATGCCGGGAATGGGCGGAATGCCACCAGGAATGATGTAA

Upstream 100 bases:

>100_bases
AAAGGTTGATGGGATGAGTTTATATAGAAGTACAAACATGTAAAATGCGATTTTAATTCCAGAGCAATTCCAAACTCACA
CTAATAAGGAGGATTAAATA

Downstream 100 bases:

>100_bases
ATTCCTGAAAAAATGAGAAAAACCGAAAACTGATTGAATCCAGAAAAGGTGCAGTATCTTTACTGCTCCTTTTTTAAAAT
TCAGTCAGAAAATGCTCCAG

Product: thermosome subunit alpha

Products: NA

Alternate protein names: Chaperonin subunit beta; Thermosome subunit 2 [H]

Number of amino acids: Translated: 551; Mature: 550

Protein sequence:

>551_residues
MAGQPIFILKEGSKRTRGRDAQSNNIMAAKAVAEAVRTTLGPKGMDKMLVDSMGDVVITNDGATILKEMDIEHPAAKMVV
EVSKTQDDEVGDGTTSAAVVAGELLNKAEDLIEQEIHPTIIASGYRLAAEKAIEVLNSLAMSVDMGNRDLLLSIAETAMT
GKGAESSKKLLAEIAVDAVTSVVDTNGKMSVDKENISVVKKVGGKTEDSELIPGMIIDKERVHTNMPEKVKDAKIALLNT
AIELKDTEVDAEISITSPDQLQSFLDQEEQMLKKIVQKVINSGANVVFCQKGVEDLAQHYLAKAGIFAVRRVKKSDMEKL
ARATGGKLITNLDEIVPEDLGFAKLVEEKKVGGDSMTFVTGCDNPKAVTILLRGGTEHVVDSIDSALEDALRVVGVAIED
EKLVAGGGSPEVEVALRLQEYAATLEGREQLAVKAYSEALEVIPRTLAENAGLDPIDMLMELRSQHEKGMKTAGLNVYEG
KVVDMWENFVVEPLRVKTQVINAATESAVMILRIDDIIASTRAAGPSPEEMGGMPGMGGMPGMGGMPPGMM

Sequences:

>Translated_551_residues
MAGQPIFILKEGSKRTRGRDAQSNNIMAAKAVAEAVRTTLGPKGMDKMLVDSMGDVVITNDGATILKEMDIEHPAAKMVV
EVSKTQDDEVGDGTTSAAVVAGELLNKAEDLIEQEIHPTIIASGYRLAAEKAIEVLNSLAMSVDMGNRDLLLSIAETAMT
GKGAESSKKLLAEIAVDAVTSVVDTNGKMSVDKENISVVKKVGGKTEDSELIPGMIIDKERVHTNMPEKVKDAKIALLNT
AIELKDTEVDAEISITSPDQLQSFLDQEEQMLKKIVQKVINSGANVVFCQKGVEDLAQHYLAKAGIFAVRRVKKSDMEKL
ARATGGKLITNLDEIVPEDLGFAKLVEEKKVGGDSMTFVTGCDNPKAVTILLRGGTEHVVDSIDSALEDALRVVGVAIED
EKLVAGGGSPEVEVALRLQEYAATLEGREQLAVKAYSEALEVIPRTLAENAGLDPIDMLMELRSQHEKGMKTAGLNVYEG
KVVDMWENFVVEPLRVKTQVINAATESAVMILRIDDIIASTRAAGPSPEEMGGMPGMGGMPGMGGMPPGMM
>Mature_550_residues
AGQPIFILKEGSKRTRGRDAQSNNIMAAKAVAEAVRTTLGPKGMDKMLVDSMGDVVITNDGATILKEMDIEHPAAKMVVE
VSKTQDDEVGDGTTSAAVVAGELLNKAEDLIEQEIHPTIIASGYRLAAEKAIEVLNSLAMSVDMGNRDLLLSIAETAMTG
KGAESSKKLLAEIAVDAVTSVVDTNGKMSVDKENISVVKKVGGKTEDSELIPGMIIDKERVHTNMPEKVKDAKIALLNTA
IELKDTEVDAEISITSPDQLQSFLDQEEQMLKKIVQKVINSGANVVFCQKGVEDLAQHYLAKAGIFAVRRVKKSDMEKLA
RATGGKLITNLDEIVPEDLGFAKLVEEKKVGGDSMTFVTGCDNPKAVTILLRGGTEHVVDSIDSALEDALRVVGVAIEDE
KLVAGGGSPEVEVALRLQEYAATLEGREQLAVKAYSEALEVIPRTLAENAGLDPIDMLMELRSQHEKGMKTAGLNVYEGK
VVDMWENFVVEPLRVKTQVINAATESAVMILRIDDIIASTRAAGPSPEEMGGMPGMGGMPGMGGMPPGMM

Specific function: Molecular chaperone; binds unfolded polypeptides in vitro, and has a weak ATPase activity [H]

COG id: COG0459

COG function: function code O; Chaperonin GroEL (HSP60 family)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TCP-1 chaperonin family [H]

Homologues:

Organism=Homo sapiens, GI63162572, Length=542, Percent_Identity=41.1439114391144, Blast_Score=437, Evalue=1e-122,
Organism=Homo sapiens, GI38455427, Length=511, Percent_Identity=41.6829745596869, Blast_Score=409, Evalue=1e-114,
Organism=Homo sapiens, GI24307939, Length=534, Percent_Identity=42.1348314606742, Blast_Score=396, Evalue=1e-110,
Organism=Homo sapiens, GI5453607, Length=535, Percent_Identity=37.0093457943925, Blast_Score=373, Evalue=1e-103,
Organism=Homo sapiens, GI58761484, Length=542, Percent_Identity=37.0848708487085, Blast_Score=372, Evalue=1e-103,
Organism=Homo sapiens, GI57863257, Length=521, Percent_Identity=37.0441458733205, Blast_Score=352, Evalue=8e-97,
Organism=Homo sapiens, GI261399877, Length=491, Percent_Identity=36.0488798370672, Blast_Score=331, Evalue=1e-90,
Organism=Homo sapiens, GI5453603, Length=522, Percent_Identity=35.823754789272, Blast_Score=311, Evalue=1e-84,
Organism=Homo sapiens, GI4502643, Length=516, Percent_Identity=33.7209302325581, Blast_Score=306, Evalue=4e-83,
Organism=Homo sapiens, GI58331173, Length=509, Percent_Identity=34.5776031434185, Blast_Score=305, Evalue=7e-83,
Organism=Homo sapiens, GI261399875, Length=446, Percent_Identity=33.8565022421525, Blast_Score=276, Evalue=3e-74,
Organism=Homo sapiens, GI48762932, Length=528, Percent_Identity=35.2272727272727, Blast_Score=269, Evalue=4e-72,
Organism=Homo sapiens, GI302058290, Length=504, Percent_Identity=34.3253968253968, Blast_Score=267, Evalue=2e-71,
Organism=Homo sapiens, GI58331171, Length=516, Percent_Identity=29.8449612403101, Blast_Score=244, Evalue=2e-64,
Organism=Homo sapiens, GI302058292, Length=509, Percent_Identity=31.041257367387, Blast_Score=243, Evalue=4e-64,
Organism=Homo sapiens, GI58331185, Length=324, Percent_Identity=34.5679012345679, Blast_Score=223, Evalue=3e-58,
Organism=Homo sapiens, GI57863259, Length=365, Percent_Identity=31.7808219178082, Blast_Score=198, Evalue=1e-50,
Organism=Homo sapiens, GI7657253, Length=509, Percent_Identity=26.5225933202358, Blast_Score=160, Evalue=4e-39,
Organism=Homo sapiens, GI121583483, Length=219, Percent_Identity=29.2237442922374, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI41399285, Length=537, Percent_Identity=24.2085661080074, Blast_Score=72, Evalue=1e-12,
Organism=Homo sapiens, GI31542947, Length=537, Percent_Identity=24.2085661080074, Blast_Score=72, Evalue=1e-12,
Organism=Escherichia coli, GI1790586, Length=542, Percent_Identity=24.5387453874539, Blast_Score=80, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI25144674, Length=536, Percent_Identity=41.7910447761194, Blast_Score=390, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17532603, Length=508, Percent_Identity=41.7322834645669, Blast_Score=375, Evalue=1e-104,
Organism=Caenorhabditis elegans, GI25148561, Length=539, Percent_Identity=36.9202226345083, Blast_Score=365, Evalue=1e-101,
Organism=Caenorhabditis elegans, GI17532601, Length=533, Percent_Identity=38.0863039399625, Blast_Score=363, Evalue=1e-100,
Organism=Caenorhabditis elegans, GI17564182, Length=538, Percent_Identity=36.6171003717472, Blast_Score=350, Evalue=9e-97,
Organism=Caenorhabditis elegans, GI25147750, Length=525, Percent_Identity=34.4761904761905, Blast_Score=292, Evalue=3e-79,
Organism=Caenorhabditis elegans, GI25144678, Length=533, Percent_Identity=33.9587242026266, Blast_Score=288, Evalue=4e-78,
Organism=Caenorhabditis elegans, GI32566944, Length=444, Percent_Identity=35.3603603603604, Blast_Score=278, Evalue=8e-75,
Organism=Caenorhabditis elegans, GI71998178, Length=559, Percent_Identity=29.1592128801431, Blast_Score=244, Evalue=1e-64,
Organism=Caenorhabditis elegans, GI25144680, Length=392, Percent_Identity=35.4591836734694, Blast_Score=241, Evalue=9e-64,
Organism=Caenorhabditis elegans, GI71981457, Length=335, Percent_Identity=37.910447761194, Blast_Score=214, Evalue=7e-56,
Organism=Caenorhabditis elegans, GI17555558, Length=548, Percent_Identity=23.5401459854015, Blast_Score=71, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322446, Length=528, Percent_Identity=38.4469696969697, Blast_Score=374, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6322350, Length=548, Percent_Identity=37.956204379562, Blast_Score=358, Evalue=1e-99,
Organism=Saccharomyces cerevisiae, GI6320058, Length=505, Percent_Identity=38.8118811881188, Blast_Score=346, Evalue=7e-96,
Organism=Saccharomyces cerevisiae, GI6320418, Length=530, Percent_Identity=35.0943396226415, Blast_Score=332, Evalue=7e-92,
Organism=Saccharomyces cerevisiae, GI6322524, Length=535, Percent_Identity=37.196261682243, Blast_Score=332, Evalue=1e-91,
Organism=Saccharomyces cerevisiae, GI6322049, Length=510, Percent_Identity=35.0980392156863, Blast_Score=286, Evalue=6e-78,
Organism=Saccharomyces cerevisiae, GI6320393, Length=524, Percent_Identity=29.3893129770992, Blast_Score=262, Evalue=1e-70,
Organism=Saccharomyces cerevisiae, GI6322452, Length=554, Percent_Identity=30.6859205776173, Blast_Score=248, Evalue=2e-66,
Organism=Drosophila melanogaster, GI24583944, Length=516, Percent_Identity=40.6976744186046, Blast_Score=390, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24647512, Length=532, Percent_Identity=40.0375939849624, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24647510, Length=532, Percent_Identity=40.0375939849624, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI17647245, Length=522, Percent_Identity=40.4214559386973, Blast_Score=371, Evalue=1e-103,
Organism=Drosophila melanogaster, GI24649027, Length=521, Percent_Identity=38.5796545105566, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24649029, Length=521, Percent_Identity=38.5796545105566, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24652903, Length=501, Percent_Identity=40.3193612774451, Blast_Score=358, Evalue=5e-99,
Organism=Drosophila melanogaster, GI24645179, Length=528, Percent_Identity=37.6893939393939, Blast_Score=356, Evalue=2e-98,
Organism=Drosophila melanogaster, GI18858175, Length=526, Percent_Identity=35.361216730038, Blast_Score=314, Evalue=1e-85,
Organism=Drosophila melanogaster, GI28571140, Length=526, Percent_Identity=35.361216730038, Blast_Score=314, Evalue=1e-85,
Organism=Drosophila melanogaster, GI18859933, Length=527, Percent_Identity=33.5863377609108, Blast_Score=288, Evalue=1e-77,
Organism=Drosophila melanogaster, GI19921848, Length=534, Percent_Identity=32.3970037453184, Blast_Score=258, Evalue=7e-69,
Organism=Drosophila melanogaster, GI20130093, Length=188, Percent_Identity=28.1914893617021, Blast_Score=70, Evalue=4e-12,
Organism=Drosophila melanogaster, GI45552711, Length=188, Percent_Identity=28.1914893617021, Blast_Score=70, Evalue=4e-12,

Paralogues:

None

Copy number: 2180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 480 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 15012 Molecules/Cell In: Growth Phase,

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017998
- InterPro:   IPR002194
- InterPro:   IPR002423
- InterPro:   IPR012714 [H]

Pfam domain/function: PF00118 Cpn60_TCP1 [H]

EC number: NA

Molecular weight: Translated: 58881; Mature: 58749

Theoretical pI: Translated: 4.53; Mature: 4.53

Prosite motif: PS00750 TCP1_1 ; PS00751 TCP1_2 ; PS00995 TCP1_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGQPIFILKEGSKRTRGRDAQSNNIMAAKAVAEAVRTTLGPKGMDKMLVDSMGDVVITN
CCCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEC
DGATILKEMDIEHPAAKMVVEVSKTQDDEVGDGTTSAAVVAGELLNKAEDLIEQEIHPTI
CCHHHHHHHCCCCCHHHHEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCE
IASGYRLAAEKAIEVLNSLAMSVDMGNRDLLLSIAETAMTGKGAESSKKLLAEIAVDAVT
EECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
SVVDTNGKMSVDKENISVVKKVGGKTEDSELIPGMIIDKERVHTNMPEKVKDAKIALLNT
HHHCCCCCEEECHHHHHHHHHHCCCCCCCCCCCCEEECHHHHHCCCCHHHHHHHEEEEHH
AIELKDTEVDAEISITSPDQLQSFLDQEEQMLKKIVQKVINSGANVVFCQKGVEDLAQHY
EEEEECCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHH
LAKAGIFAVRRVKKSDMEKLARATGGKLITNLDEIVPEDLGFAKLVEEKKVGGDSMTFVT
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEE
GCDNPKAVTILLRGGTEHVVDSIDSALEDALRVVGVAIEDEKLVAGGGSPEVEVALRLQE
CCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHEEEECCEEEECCCCCCEEHHHHHHH
YAATLEGREQLAVKAYSEALEVIPRTLAENAGLDPIDMLMELRSQHEKGMKTAGLNVYEG
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHCCCEEECC
KVVDMWENFVVEPLRVKTQVINAATESAVMILRIDDIIASTRAAGPSPEEMGGMPGMGGM
HHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEEEHHHHHHHCCCCCCHHHHCCCCCCCCC
PGMGGMPPGMM
CCCCCCCCCCC
>Mature Secondary Structure 
AGQPIFILKEGSKRTRGRDAQSNNIMAAKAVAEAVRTTLGPKGMDKMLVDSMGDVVITN
CCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCEEEEC
DGATILKEMDIEHPAAKMVVEVSKTQDDEVGDGTTSAAVVAGELLNKAEDLIEQEIHPTI
CCHHHHHHHCCCCCHHHHEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCE
IASGYRLAAEKAIEVLNSLAMSVDMGNRDLLLSIAETAMTGKGAESSKKLLAEIAVDAVT
EECCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
SVVDTNGKMSVDKENISVVKKVGGKTEDSELIPGMIIDKERVHTNMPEKVKDAKIALLNT
HHHCCCCCEEECHHHHHHHHHHCCCCCCCCCCCCEEECHHHHHCCCCHHHHHHHEEEEHH
AIELKDTEVDAEISITSPDQLQSFLDQEEQMLKKIVQKVINSGANVVFCQKGVEDLAQHY
EEEEECCCCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHH
LAKAGIFAVRRVKKSDMEKLARATGGKLITNLDEIVPEDLGFAKLVEEKKVGGDSMTFVT
HHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHCCCCCEEEEE
GCDNPKAVTILLRGGTEHVVDSIDSALEDALRVVGVAIEDEKLVAGGGSPEVEVALRLQE
CCCCCCEEEEEEECCHHHHHHHHHHHHHHHHHHHHEEEECCEEEECCCCCCEEHHHHHHH
YAATLEGREQLAVKAYSEALEVIPRTLAENAGLDPIDMLMELRSQHEKGMKTAGLNVYEG
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCHHCCCEEECC
KVVDMWENFVVEPLRVKTQVINAATESAVMILRIDDIIASTRAAGPSPEEMGGMPGMGGM
HHHHHHHHHHCCHHHHHHHHHHHHCCCEEEEEEEHHHHHHHCCCCCCHHHHCCCCCCCCC
PGMGGMPPGMM
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9389475 [H]