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The map label for this gene is gatB [H]

Identifier: 21227330

GI number: 21227330

Start: 1454951

End: 1456438

Strand: Direct

Name: gatB [H]

Synonym: MM_1228

Alternate gene names: 21227330

Gene position: 1454951-1456438 (Clockwise)

Preceding gene: 21227329

Following gene: 21227333

Centisome position: 35.52

GC content: 49.6

Gene sequence:

>1488_bases
ATGGTCTATGAAAACCCTGACGGTATAAGGATAGGGCTTGAAATCCACGTCCAGCTTAACAAACTCAAGACCAAAATGTT
CTGCGGCTGCTCTACAGATTATCACAATGCGGCCCCCAATACCCACACCTGTCCGGTCTGCCTCGGCCTGCCGGGTGCAC
TTCCGGTCCTTAATAAAAAAGCCGTGGAAGCAGCAATTAAAGTGGGGCTTGCCCTTGAAGGTGAGATTGCAGAAGAAACC
CAGTTCCACAGGAAGAATTACTTCTATCCTGACCTCCCCAAAGGTTTCCAGGTCACCCAGTACGACTATCCGATTGTTAG
CAAAGGAAAAGTCGTGATCGAAGGAGAAGACGGGGAGCATGTGGTAGGAATTACAAGGGCTCACATGGAAGAAGACCCTG
GAAAGCTTGTACACATAGGGAGCATTGAAAAGTCCAAAGGTGTCCTTATAGACTACAACAGGTCAGGCATGCCCCTGATC
GAGACCGTTACCGAGCCGGATATGAGGAGTCCCAAAGAAGCAAGGAGATTCCTTGATAAGTTCAGAAACATCCTCGAATA
CCTGGATGTCTTTGACGGAAACCTTGAAGGAGCTATGCGTGTCGACGCGAACGTTTCTGTCCACTGGGGGACCAGGGTTG
AGGTCAAAAACATCTCTTCCCATAAAGGAGTGGAAAGAGCACTTCTCTACGAGATCATGCGCCAGAAAAACGTGATCCGC
CGCGGAGGAAAAATTACGCAGGAAACGCGTCACTTCGATGAAGGAAGGGGAGTAACCATTTCCATGAGGACAAAAGAAGA
GGCAGAAGACTACCGCTACTTCCGTGAACCTGACCTCATGCCAATGCGCGTTACAGGCTGGATACCCGCAATTAAAGAGA
CCCTTCCCGAGCTTCCGGATGCCAAACGTGCACGCTTCATGGAGCAGTACGGTATCACAGATATGCATGCAAGAGCCCTG
ACCTCAAAGATTATGCTCGCCGACTTCTACGAAGGCGTTTGCGCAAAAGGGGTAGACCCGAAGGTTGCAGCTACCTGGAC
TGCTGACGTCCTCCTCGGAGAGTTAAACTATAGAGACCTTGCAATCTCTTCTTACGACGGCAGGACAATTGGCTTTATCC
ATGCAAAAGACCCTGAAGTGGAGAATTCCTTCAAGGTCTCGGATATGGTTGAACTGGTCACACTTTTTGCCGAAGGCAAG
GTCAGCGACAGGGCTGCGGTTGAAGTCATCCGTACCATTCTGGACGGAAATGAGGAAAAGAGCCCATCGCAGATCATAGA
AGAAAAAGGCCTTTTCAAAGCCGAAGACGATCTGGTAACCAGAGCTGTTGCAGAAACAATTGCCGAAAACGAAGCTGCCG
TGCAGGACTATCTTGGAGGAACGGAAAAGTCCCTGAACTTCCTTGTAGGACAGGTCATGAAAAAGACAAAAGGCACGGCG
GACGCAAAGACAGCCCGTGAACTGATTGTAAAGGAACTTAAAGGGTAA

Upstream 100 bases:

>100_bases
AATGGGCAAGCCCTTTGACGAAGAAACTGTCCTGCGTGCAGCTTACACTTTTGAGAAGAATACCGATTACCATACCAAGA
GACCTCCGGAGGTGGCATGA

Downstream 100 bases:

>100_bases
CCCGGCAGGGTTCACCTTTTTAACTACTTTTTTAACGGTTAATAAAGGTCAGGCCTGCGGTCTTCGAGAATAGAGCCGGT
TTGTCTGATTTTTCTTGCCT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 495; Mature: 495

Protein sequence:

>495_residues
MVYENPDGIRIGLEIHVQLNKLKTKMFCGCSTDYHNAAPNTHTCPVCLGLPGALPVLNKKAVEAAIKVGLALEGEIAEET
QFHRKNYFYPDLPKGFQVTQYDYPIVSKGKVVIEGEDGEHVVGITRAHMEEDPGKLVHIGSIEKSKGVLIDYNRSGMPLI
ETVTEPDMRSPKEARRFLDKFRNILEYLDVFDGNLEGAMRVDANVSVHWGTRVEVKNISSHKGVERALLYEIMRQKNVIR
RGGKITQETRHFDEGRGVTISMRTKEEAEDYRYFREPDLMPMRVTGWIPAIKETLPELPDAKRARFMEQYGITDMHARAL
TSKIMLADFYEGVCAKGVDPKVAATWTADVLLGELNYRDLAISSYDGRTIGFIHAKDPEVENSFKVSDMVELVTLFAEGK
VSDRAAVEVIRTILDGNEEKSPSQIIEEKGLFKAEDDLVTRAVAETIAENEAAVQDYLGGTEKSLNFLVGQVMKKTKGTA
DAKTARELIVKELKG

Sequences:

>Translated_495_residues
MVYENPDGIRIGLEIHVQLNKLKTKMFCGCSTDYHNAAPNTHTCPVCLGLPGALPVLNKKAVEAAIKVGLALEGEIAEET
QFHRKNYFYPDLPKGFQVTQYDYPIVSKGKVVIEGEDGEHVVGITRAHMEEDPGKLVHIGSIEKSKGVLIDYNRSGMPLI
ETVTEPDMRSPKEARRFLDKFRNILEYLDVFDGNLEGAMRVDANVSVHWGTRVEVKNISSHKGVERALLYEIMRQKNVIR
RGGKITQETRHFDEGRGVTISMRTKEEAEDYRYFREPDLMPMRVTGWIPAIKETLPELPDAKRARFMEQYGITDMHARAL
TSKIMLADFYEGVCAKGVDPKVAATWTADVLLGELNYRDLAISSYDGRTIGFIHAKDPEVENSFKVSDMVELVTLFAEGK
VSDRAAVEVIRTILDGNEEKSPSQIIEEKGLFKAEDDLVTRAVAETIAENEAAVQDYLGGTEKSLNFLVGQVMKKTKGTA
DAKTARELIVKELKG
>Mature_495_residues
MVYENPDGIRIGLEIHVQLNKLKTKMFCGCSTDYHNAAPNTHTCPVCLGLPGALPVLNKKAVEAAIKVGLALEGEIAEET
QFHRKNYFYPDLPKGFQVTQYDYPIVSKGKVVIEGEDGEHVVGITRAHMEEDPGKLVHIGSIEKSKGVLIDYNRSGMPLI
ETVTEPDMRSPKEARRFLDKFRNILEYLDVFDGNLEGAMRVDANVSVHWGTRVEVKNISSHKGVERALLYEIMRQKNVIR
RGGKITQETRHFDEGRGVTISMRTKEEAEDYRYFREPDLMPMRVTGWIPAIKETLPELPDAKRARFMEQYGITDMHARAL
TSKIMLADFYEGVCAKGVDPKVAATWTADVLLGELNYRDLAISSYDGRTIGFIHAKDPEVENSFKVSDMVELVTLFAEGK
VSDRAAVEVIRTILDGNEEKSPSQIIEEKGLFKAEDDLVTRAVAETIAENEAAVQDYLGGTEKSLNFLVGQVMKKTKGTA
DAKTARELIVKELKG

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=512, Percent_Identity=34.9609375, Blast_Score=264, Evalue=2e-70,
Organism=Caenorhabditis elegans, GI115534094, Length=299, Percent_Identity=40.4682274247492, Blast_Score=204, Evalue=9e-53,
Organism=Saccharomyces cerevisiae, GI6319391, Length=520, Percent_Identity=29.6153846153846, Blast_Score=201, Evalue=2e-52,
Organism=Drosophila melanogaster, GI45550808, Length=509, Percent_Identity=31.434184675835, Blast_Score=228, Evalue=7e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 55370; Mature: 55370

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVYENPDGIRIGLEIHVQLNKLKTKMFCGCSTDYHNAAPNTHTCPVCLGLPGALPVLNKK
CCCCCCCCEEEEEEEEEEEEHHCCHHEECCCCCCCCCCCCCCCCEEEECCCCCCCHHHHH
AVEAAIKVGLALEGEIAEETQFHRKNYFYPDLPKGFQVTQYDYPIVSKGKVVIEGEDGEH
HHHHHHHHCEEECCCHHHHHHHHHCCCCCCCCCCCCEEEECCCCEECCCEEEEECCCCCE
VVGITRAHMEEDPGKLVHIGSIEKSKGVLIDYNRSGMPLIETVTEPDMRSPKEARRFLDK
EEEEEHHHCCCCCCCEEEEECCCCCCCEEEEECCCCCCCEEECCCCCCCCHHHHHHHHHH
FRNILEYLDVFDGNLEGAMRVDANVSVHWGTRVEVKNISSHKGVERALLYEIMRQKNVIR
HHHHHHHHHHHCCCCCCEEEECCEEEEEECCEEEEECCCCCCCHHHHHHHHHHHHHHHHH
RGGKITQETRHFDEGRGVTISMRTKEEAEDYRYFREPDLMPMRVTGWIPAIKETLPELPD
CCCCCCHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHCCCCC
AKRARFMEQYGITDMHARALTSKIMLADFYEGVCAKGVDPKVAATWTADVLLGELNYRDL
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHEECCCCCEE
AISSYDGRTIGFIHAKDPEVENSFKVSDMVELVTLFAEGKVSDRAAVEVIRTILDGNEEK
EEECCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCC
SPSQIIEEKGLFKAEDDLVTRAVAETIAENEAAVQDYLGGTEKSLNFLVGQVMKKTKGTA
CHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
DAKTARELIVKELKG
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MVYENPDGIRIGLEIHVQLNKLKTKMFCGCSTDYHNAAPNTHTCPVCLGLPGALPVLNKK
CCCCCCCCEEEEEEEEEEEEHHCCHHEECCCCCCCCCCCCCCCCEEEECCCCCCCHHHHH
AVEAAIKVGLALEGEIAEETQFHRKNYFYPDLPKGFQVTQYDYPIVSKGKVVIEGEDGEH
HHHHHHHHCEEECCCHHHHHHHHHCCCCCCCCCCCCEEEECCCCEECCCEEEEECCCCCE
VVGITRAHMEEDPGKLVHIGSIEKSKGVLIDYNRSGMPLIETVTEPDMRSPKEARRFLDK
EEEEEHHHCCCCCCCEEEEECCCCCCCEEEEECCCCCCCEEECCCCCCCCHHHHHHHHHH
FRNILEYLDVFDGNLEGAMRVDANVSVHWGTRVEVKNISSHKGVERALLYEIMRQKNVIR
HHHHHHHHHHHCCCCCCEEEECCEEEEEECCEEEEECCCCCCCHHHHHHHHHHHHHHHHH
RGGKITQETRHFDEGRGVTISMRTKEEAEDYRYFREPDLMPMRVTGWIPAIKETLPELPD
CCCCCCHHHHHCCCCCCEEEEECCHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHCCCCC
AKRARFMEQYGITDMHARALTSKIMLADFYEGVCAKGVDPKVAATWTADVLLGELNYRDL
HHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEHHHHEECCCCCEE
AISSYDGRTIGFIHAKDPEVENSFKVSDMVELVTLFAEGKVSDRAAVEVIRTILDGNEEK
EEECCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCCC
SPSQIIEEKGLFKAEDDLVTRAVAETIAENEAAVQDYLGGTEKSLNFLVGQVMKKTKGTA
CHHHHHHHCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
DAKTARELIVKELKG
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]