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The map label for this gene is gatA [H]

Identifier: 21227329

GI number: 21227329

Start: 1453520

End: 1454950

Strand: Direct

Name: gatA [H]

Synonym: MM_1227

Alternate gene names: 21227329

Gene position: 1453520-1454950 (Clockwise)

Preceding gene: 21227328

Following gene: 21227330

Centisome position: 35.48

GC content: 47.73

Gene sequence:

>1431_bases
TTGATGGCAAAATGGATGAGTGTTGCACAGGTTAAAGAAAAGATTAAAGAAAGTTCAGCCGAAGAAGTGACATCCAGATA
CCTTGAAGTTATCAAAAAAAGCAAAATCAACGGATATCTAACGATTTCCGATAAGGCTCTTGAGCAGGCAAAAAAGATCG
ATAAAGAAGGGCATGAAGGCCCTCTTGCAGGCGTACCGATTGCTATTAAGGATAACATTTCTGTTGTCGGGCTGCCAAAC
AGCTGTGGCTCGAAGATCCTTGAAGGCTATGTCCCTCCTTTCAATGCCCACGTCATAGAGAAACTTCTTTCTGCAGGAGC
AGTGATCCTTGGAAAAACCAATATGGACGAATTTGCCATGGGATCTTCCACTGAAACAAGTCATTTCGGGCCGACTGCAA
ACCCCTGGGACCTTGAAAGAGTGCCAGGCGGGTCTTCCGGTGGAAGTGCAGCAGTTGTAGCAGCAGGAGAAGCTCCTTTT
GCTCTCGGTTCCGACACAGGCGGATCTGTACGCTGTCCTGCATCTTTCTGTGGGGTTGTAGGGCTTAAACCGACCTATGG
AGCAGTTTCAAGGTACGGGGTTGTGGCTTATGCAAATTCCCTTGAACAGGTTGGACCCCTCGCAAACAACGTGGAAGACA
TAGCAGTCCTGATGGATGTTATAGCCGGATATGATAGAAGAGATTCCACTTCAATTGACAGCAAAACCGAGTACCAGAAA
GCTCTTGTTGATGACGTGAAGGGCCTGAAAATAGGGGTTCCGAAGGAATTCTTCGGGGAAGGCATCCATCCTGACGTAGA
AAAAGCAGTATGGGATGCAATACACAAATGTGAGAGCCTCGGAGCGACGTGGGAAGAAGTCTCAATGCCCCATATAAAGT
ATGCCCTTGCCTCATATTATATAATTGCAATGAGTGAAGCGTCCTCAAACCTTGCCCGCTTCGATGGAACACGTTACGGA
TTCAGAGCAGGCGGAGAAAACTGGCATGCTATGGTTTCAAAGACCAGAGCTGAAGGTTTCGGCACAGAAGTCAAGAGAAG
AATTCTCCTCGGAACCTATGCTCTTTCTGCAGGTTATCATGACAAATACTATCTCAAAGCCCTGAAGGTCAGGACTCTTG
TGAAGCAGGACTTTGATAAAGCCCTTTCAAAAGTTGATCTGCTCATGGCTCCGACCATGCCGAACCCTGCTTTTAAGATC
GGAGAAAAGATTGAAGACCCGCTTACCCTCTACCTTTCAGACATAAACACCTGCCCGATTAACTTGGCAGGCGTGCCTTC
GATCTCCGTGCCCTGCGGATTTACGGACGGGCTGCCGATAGGACTTCAGATAATGGGCAAGCCCTTTGACGAAGAAACTG
TCCTGCGTGCAGCTTACACTTTTGAGAAGAATACCGATTACCATACCAAGAGACCTCCGGAGGTGGCATGA

Upstream 100 bases:

>100_bases
TGTGGCGGAAGAATGCCTCCCGCAAGAAACTGTTCTTGCAAATACCGAGCACAAACAGGACGGGACTTTCAGGGTCCCGA
AAATCGGCTGAGGAGATGTT

Downstream 100 bases:

>100_bases
ATGGTCTATGAAAACCCTGACGGTATAAGGATAGGGCTTGAAATCCACGTCCAGCTTAACAAACTCAAGACCAAAATGTT
CTGCGGCTGCTCTACAGATT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 476; Mature: 476

Protein sequence:

>476_residues
MMAKWMSVAQVKEKIKESSAEEVTSRYLEVIKKSKINGYLTISDKALEQAKKIDKEGHEGPLAGVPIAIKDNISVVGLPN
SCGSKILEGYVPPFNAHVIEKLLSAGAVILGKTNMDEFAMGSSTETSHFGPTANPWDLERVPGGSSGGSAAVVAAGEAPF
ALGSDTGGSVRCPASFCGVVGLKPTYGAVSRYGVVAYANSLEQVGPLANNVEDIAVLMDVIAGYDRRDSTSIDSKTEYQK
ALVDDVKGLKIGVPKEFFGEGIHPDVEKAVWDAIHKCESLGATWEEVSMPHIKYALASYYIIAMSEASSNLARFDGTRYG
FRAGGENWHAMVSKTRAEGFGTEVKRRILLGTYALSAGYHDKYYLKALKVRTLVKQDFDKALSKVDLLMAPTMPNPAFKI
GEKIEDPLTLYLSDINTCPINLAGVPSISVPCGFTDGLPIGLQIMGKPFDEETVLRAAYTFEKNTDYHTKRPPEVA

Sequences:

>Translated_476_residues
MMAKWMSVAQVKEKIKESSAEEVTSRYLEVIKKSKINGYLTISDKALEQAKKIDKEGHEGPLAGVPIAIKDNISVVGLPN
SCGSKILEGYVPPFNAHVIEKLLSAGAVILGKTNMDEFAMGSSTETSHFGPTANPWDLERVPGGSSGGSAAVVAAGEAPF
ALGSDTGGSVRCPASFCGVVGLKPTYGAVSRYGVVAYANSLEQVGPLANNVEDIAVLMDVIAGYDRRDSTSIDSKTEYQK
ALVDDVKGLKIGVPKEFFGEGIHPDVEKAVWDAIHKCESLGATWEEVSMPHIKYALASYYIIAMSEASSNLARFDGTRYG
FRAGGENWHAMVSKTRAEGFGTEVKRRILLGTYALSAGYHDKYYLKALKVRTLVKQDFDKALSKVDLLMAPTMPNPAFKI
GEKIEDPLTLYLSDINTCPINLAGVPSISVPCGFTDGLPIGLQIMGKPFDEETVLRAAYTFEKNTDYHTKRPPEVA
>Mature_476_residues
MMAKWMSVAQVKEKIKESSAEEVTSRYLEVIKKSKINGYLTISDKALEQAKKIDKEGHEGPLAGVPIAIKDNISVVGLPN
SCGSKILEGYVPPFNAHVIEKLLSAGAVILGKTNMDEFAMGSSTETSHFGPTANPWDLERVPGGSSGGSAAVVAAGEAPF
ALGSDTGGSVRCPASFCGVVGLKPTYGAVSRYGVVAYANSLEQVGPLANNVEDIAVLMDVIAGYDRRDSTSIDSKTEYQK
ALVDDVKGLKIGVPKEFFGEGIHPDVEKAVWDAIHKCESLGATWEEVSMPHIKYALASYYIIAMSEASSNLARFDGTRYG
FRAGGENWHAMVSKTRAEGFGTEVKRRILLGTYALSAGYHDKYYLKALKVRTLVKQDFDKALSKVDLLMAPTMPNPAFKI
GEKIEDPLTLYLSDINTCPINLAGVPSISVPCGFTDGLPIGLQIMGKPFDEETVLRAAYTFEKNTDYHTKRPPEVA

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=500, Percent_Identity=38.4, Blast_Score=321, Evalue=8e-88,
Organism=Homo sapiens, GI195972892, Length=488, Percent_Identity=25.6147540983607, Blast_Score=130, Evalue=3e-30,
Organism=Homo sapiens, GI166795287, Length=181, Percent_Identity=35.3591160220994, Blast_Score=80, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI17543272, Length=431, Percent_Identity=39.907192575406, Blast_Score=291, Evalue=7e-79,
Organism=Caenorhabditis elegans, GI17537465, Length=493, Percent_Identity=25.9634888438134, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17556264, Length=281, Percent_Identity=30.2491103202847, Blast_Score=115, Evalue=7e-26,
Organism=Caenorhabditis elegans, GI71990152, Length=317, Percent_Identity=28.391167192429, Blast_Score=100, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17538252, Length=488, Percent_Identity=23.155737704918, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17556276, Length=159, Percent_Identity=38.9937106918239, Blast_Score=79, Evalue=7e-15,
Organism=Caenorhabditis elegans, GI17556278, Length=159, Percent_Identity=38.9937106918239, Blast_Score=78, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI32565337, Length=439, Percent_Identity=26.4236902050114, Blast_Score=72, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17538254, Length=276, Percent_Identity=25.7246376811594, Blast_Score=65, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=428, Percent_Identity=38.5514018691589, Blast_Score=248, Evalue=1e-66,
Organism=Saccharomyces cerevisiae, GI6319685, Length=479, Percent_Identity=31.3152400835073, Blast_Score=136, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6320448, Length=170, Percent_Identity=34.1176470588235, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI24648113, Length=425, Percent_Identity=40.2352941176471, Blast_Score=284, Evalue=7e-77,
Organism=Drosophila melanogaster, GI24652985, Length=497, Percent_Identity=25.7545271629779, Blast_Score=128, Evalue=8e-30,
Organism=Drosophila melanogaster, GI19922090, Length=497, Percent_Identity=25.7545271629779, Blast_Score=128, Evalue=8e-30,
Organism=Drosophila melanogaster, GI24652981, Length=497, Percent_Identity=25.7545271629779, Blast_Score=128, Evalue=8e-30,
Organism=Drosophila melanogaster, GI24652983, Length=497, Percent_Identity=25.7545271629779, Blast_Score=128, Evalue=8e-30,
Organism=Drosophila melanogaster, GI24644968, Length=488, Percent_Identity=25.2049180327869, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI161078093, Length=466, Percent_Identity=27.2532188841202, Blast_Score=121, Evalue=9e-28,
Organism=Drosophila melanogaster, GI24648435, Length=495, Percent_Identity=25.0505050505051, Blast_Score=119, Evalue=7e-27,
Organism=Drosophila melanogaster, GI24648437, Length=495, Percent_Identity=25.0505050505051, Blast_Score=119, Evalue=7e-27,
Organism=Drosophila melanogaster, GI45550774, Length=493, Percent_Identity=25.5578093306288, Blast_Score=117, Evalue=2e-26,
Organism=Drosophila melanogaster, GI21356731, Length=210, Percent_Identity=31.4285714285714, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24648441, Length=434, Percent_Identity=25.3456221198157, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24648439, Length=434, Percent_Identity=25.3456221198157, Blast_Score=110, Evalue=2e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 51335; Mature: 51335

Theoretical pI: Translated: 6.26; Mature: 6.26

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMAKWMSVAQVKEKIKESSAEEVTSRYLEVIKKSKINGYLTISDKALEQAKKIDKEGHEG
CCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHCCCCCC
PLAGVPIAIKDNISVVGLPNSCGSKILEGYVPPFNAHVIEKLLSAGAVILGKTNMDEFAM
CCCCCCEEEECCEEEEECCCHHHHHHHHCCCCCCCHHHHHHHHHCCEEEEECCCCHHHHC
GSSTETSHFGPTANPWDLERVPGGSSGGSAAVVAAGEAPFALGSDTGGSVRCPASFCGVV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEECCCCCCCEECCHHHHCCC
GLKPTYGAVSRYGVVAYANSLEQVGPLANNVEDIAVLMDVIAGYDRRDSTSIDSKTEYQK
CCCCCHHHHHHHHHHHHHHHHHHHCHHHCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
ALVDDVKGLKIGVPKEFFGEGIHPDVEKAVWDAIHKCESLGATWEEVSMPHIKYALASYY
HHHHHHCCCEECCCHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHEE
IIAMSEASSNLARFDGTRYGFRAGGENWHAMVSKTRAEGFGTEVKRRILLGTYALSAGYH
EEEEECCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
DKYYLKALKVRTLVKQDFDKALSKVDLLMAPTMPNPAFKIGEKIEDPLTLYLSDINTCPI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCE
NLAGVPSISVPCGFTDGLPIGLQIMGKPFDEETVLRAAYTFEKNTDYHTKRPPEVA
EECCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MMAKWMSVAQVKEKIKESSAEEVTSRYLEVIKKSKINGYLTISDKALEQAKKIDKEGHEG
CCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCEEEEECHHHHHHHHHHHCCCCCC
PLAGVPIAIKDNISVVGLPNSCGSKILEGYVPPFNAHVIEKLLSAGAVILGKTNMDEFAM
CCCCCCEEEECCEEEEECCCHHHHHHHHCCCCCCCHHHHHHHHHCCEEEEECCCCHHHHC
GSSTETSHFGPTANPWDLERVPGGSSGGSAAVVAAGEAPFALGSDTGGSVRCPASFCGVV
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEECCCCEEECCCCCCCEECCHHHHCCC
GLKPTYGAVSRYGVVAYANSLEQVGPLANNVEDIAVLMDVIAGYDRRDSTSIDSKTEYQK
CCCCCHHHHHHHHHHHHHHHHHHHCHHHCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHH
ALVDDVKGLKIGVPKEFFGEGIHPDVEKAVWDAIHKCESLGATWEEVSMPHIKYALASYY
HHHHHHCCCEECCCHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHEE
IIAMSEASSNLARFDGTRYGFRAGGENWHAMVSKTRAEGFGTEVKRRILLGTYALSAGYH
EEEEECCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
DKYYLKALKVRTLVKQDFDKALSKVDLLMAPTMPNPAFKIGEKIEDPLTLYLSDINTCPI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCCEEEEECCCCCCE
NLAGVPSISVPCGFTDGLPIGLQIMGKPFDEETVLRAAYTFEKNTDYHTKRPPEVA
EECCCCCCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]