Click here to switch to the map view.

The map label for this gene is hycE [H]

Identifier: 21227165

GI number: 21227165

Start: 1247405

End: 1248997

Strand: Direct

Name: hycE [H]

Synonym: MM_1063

Alternate gene names: 21227165

Gene position: 1247405-1248997 (Clockwise)

Preceding gene: 21227164

Following gene: 21227166

Centisome position: 30.45

GC content: 48.65

Gene sequence:

>1593_bases
TTGCTTTCTACAGTCTCCAGCATCGCACAGGAGAAGCTTGAAATTCTCAGCACCCGTGAAAACGAGATATACCTGAGAAT
TTCGGAAACCGGGTTTAAAGAGGTCTGCCCTGCACTTGCGGAAAGGAAGTTTAGCCTGATAGGACTTTTCTGTGCGGAAG
CCTTTGAGGGAAAGGACAACTTTACCCTATTTTATGCCTTCAAGAAAGGAGGAATGTCACCGGTCCTTATTCTCGTCAGG
GAAACAGGAAATGAAAAAAGGATTACTTCGATTGCAGAAACTTTTCCCTCAGCATCCTGGTTTGAAAGGGAGGTCAGGGA
CGGTTTCGGCATTGAATTTGACGGAGCTTTTGATACACGAAGGCTTTTCCTGCACGAGTGTTATCCTGAAGGTTTCCACC
CCCTCCTGAAATCCTTCAAAAACGGACCAATCAGCCCGGTTGAGGCTCCGCATAAGGAGTACAAATTCAGGCAGCACAAA
GGGGAGGGTGTATATCAGATCCCCGTCGGGCCGGTGCATGCCGGGATAATTGAGCCGGGACATTTCAGGTTCAGTGTGAT
AGGAGAGCCTATATTCAGCCTGGAGATCCGTCTTTTTTACAAGCACAGGGGAATAGAAAAGCTGGCTGAAGGAAAAAACC
CTTCCGAATGTGTTGCTCTCGCAGAAGCCGTAAGCGGGGATGAGAGCATGGCAAACGCAGCAGGATTCTGTATGGCAGTG
GAACAGGTCTGCGGGATAAAGGTGCCTGAAAGGGCGGAACGCCTGAGGGCGGTAATGCTCGAACTGGAGCGCATTTACTC
CCTGCTCGGAGACCTCGCAGGAATGGCTGTAGATGTGGGTTTCGCGCTTGTAGCAAGTCCGTTCTTCATATTGAGAGAAG
AAGTTTTCAGGCTGAACGAGAAGCTAACGGGTTCAAGGTTCCTTCGCGGGATCACCTTTCCCGGAGGGTTGAAAAAAGAC
ATTCCAGAATCGGCTCTGAAAGAAATTCCTGAATTTCTTGGGAAGTTTTCCAGGTCTTTTGAATCCGCTTATAACAGGGC
AACATCTTCGTCTTCCCTGATTGACAGGTTTGTAACGACCGGAGTGATAAAGAAAGAACTTATTTCTCCGCTTAACCTTA
CGGGCCCTATTGCCAGAGCTTCTGGGTCCTCTTCGGATACCAGGCTTGACAGGCCTTACGGAGCCTACAGGAACTTTACT
CCAGAGCACTGCCTGAGGAAAAAAGGAGATGTTTTTTCCCGTTTTGAGATAAAAGCCAGTGAAATCCGTGCAGCTGTAGG
CCTGATCCTGCGTCTCACGGAAAAACTCCCGCAGGGTCCCGTACTTGCTGAGAACAGCGGAAGTGGAGAAAGTGCCGGAG
AGATAAACATTTCCGGAACTACAGGATATTCCCTTTCCCTGGTAGAAGCCCCAAGGGGACAGAACCTTCACTGGGTATAT
CTTAAAAACGGGATTGTTGACCGTTATAAGGTAAGGACGGCATCTTTCTGCAACTGGCAGGCTATAGAACATGCTGTTCT
TGGAAATATTGTTCCTGATTTCCCCCTGATAAATAAGAGCCTTAACCTCTCCTATGCAGGAACGGACCTTTGA

Upstream 100 bases:

>100_bases
CGGACTCCCTGAAGGATCTGCTTGATGCGATAATTCTCGAACTGGGATATGTTTAAGGGGCGTATGTATGGACATCAAAA
TGAACGAAGATATAACAGAA

Downstream 100 bases:

>100_bases
AAAGAGAAAAAGGCAGTTGAAGGTTCAAGCAAAAAAACAGTGAACAGATTCAATTAAAGAATATAAAGAAAAAGTGAGGT
CTTATATGGTAAACCCTCTT

Product: formate hydrogenlyase subunit 5

Products: CO2; H2; oxidized ferredoxin + [C]

Alternate protein names: FHL subunit 5; Hydrogenase-3 component E [H]

Number of amino acids: Translated: 530; Mature: 530

Protein sequence:

>530_residues
MLSTVSSIAQEKLEILSTRENEIYLRISETGFKEVCPALAERKFSLIGLFCAEAFEGKDNFTLFYAFKKGGMSPVLILVR
ETGNEKRITSIAETFPSASWFEREVRDGFGIEFDGAFDTRRLFLHECYPEGFHPLLKSFKNGPISPVEAPHKEYKFRQHK
GEGVYQIPVGPVHAGIIEPGHFRFSVIGEPIFSLEIRLFYKHRGIEKLAEGKNPSECVALAEAVSGDESMANAAGFCMAV
EQVCGIKVPERAERLRAVMLELERIYSLLGDLAGMAVDVGFALVASPFFILREEVFRLNEKLTGSRFLRGITFPGGLKKD
IPESALKEIPEFLGKFSRSFESAYNRATSSSSLIDRFVTTGVIKKELISPLNLTGPIARASGSSSDTRLDRPYGAYRNFT
PEHCLRKKGDVFSRFEIKASEIRAAVGLILRLTEKLPQGPVLAENSGSGESAGEINISGTTGYSLSLVEAPRGQNLHWVY
LKNGIVDRYKVRTASFCNWQAIEHAVLGNIVPDFPLINKSLNLSYAGTDL

Sequences:

>Translated_530_residues
MLSTVSSIAQEKLEILSTRENEIYLRISETGFKEVCPALAERKFSLIGLFCAEAFEGKDNFTLFYAFKKGGMSPVLILVR
ETGNEKRITSIAETFPSASWFEREVRDGFGIEFDGAFDTRRLFLHECYPEGFHPLLKSFKNGPISPVEAPHKEYKFRQHK
GEGVYQIPVGPVHAGIIEPGHFRFSVIGEPIFSLEIRLFYKHRGIEKLAEGKNPSECVALAEAVSGDESMANAAGFCMAV
EQVCGIKVPERAERLRAVMLELERIYSLLGDLAGMAVDVGFALVASPFFILREEVFRLNEKLTGSRFLRGITFPGGLKKD
IPESALKEIPEFLGKFSRSFESAYNRATSSSSLIDRFVTTGVIKKELISPLNLTGPIARASGSSSDTRLDRPYGAYRNFT
PEHCLRKKGDVFSRFEIKASEIRAAVGLILRLTEKLPQGPVLAENSGSGESAGEINISGTTGYSLSLVEAPRGQNLHWVY
LKNGIVDRYKVRTASFCNWQAIEHAVLGNIVPDFPLINKSLNLSYAGTDL
>Mature_530_residues
MLSTVSSIAQEKLEILSTRENEIYLRISETGFKEVCPALAERKFSLIGLFCAEAFEGKDNFTLFYAFKKGGMSPVLILVR
ETGNEKRITSIAETFPSASWFEREVRDGFGIEFDGAFDTRRLFLHECYPEGFHPLLKSFKNGPISPVEAPHKEYKFRQHK
GEGVYQIPVGPVHAGIIEPGHFRFSVIGEPIFSLEIRLFYKHRGIEKLAEGKNPSECVALAEAVSGDESMANAAGFCMAV
EQVCGIKVPERAERLRAVMLELERIYSLLGDLAGMAVDVGFALVASPFFILREEVFRLNEKLTGSRFLRGITFPGGLKKD
IPESALKEIPEFLGKFSRSFESAYNRATSSSSLIDRFVTTGVIKKELISPLNLTGPIARASGSSSDTRLDRPYGAYRNFT
PEHCLRKKGDVFSRFEIKASEIRAAVGLILRLTEKLPQGPVLAENSGSGESAGEINISGTTGYSLSLVEAPRGQNLHWVY
LKNGIVDRYKVRTASFCNWQAIEHAVLGNIVPDFPLINKSLNLSYAGTDL

Specific function: Hydrogen metabolism; FHL pathway. [C]

COG id: COG3261

COG function: function code C; Ni,Fe-hydrogenase III large subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 49 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI4758786, Length=381, Percent_Identity=23.8845144356955, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI260898743, Length=365, Percent_Identity=24.3835616438356, Blast_Score=113, Evalue=5e-25,
Organism=Escherichia coli, GI1789076, Length=489, Percent_Identity=31.9018404907975, Blast_Score=249, Evalue=3e-67,
Organism=Escherichia coli, GI1788832, Length=475, Percent_Identity=32.8421052631579, Blast_Score=231, Evalue=9e-62,
Organism=Escherichia coli, GI145693162, Length=509, Percent_Identity=24.950884086444, Blast_Score=143, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17555284, Length=406, Percent_Identity=24.384236453202, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17568379, Length=349, Percent_Identity=24.6418338108883, Blast_Score=118, Evalue=6e-27,
Organism=Drosophila melanogaster, GI221459469, Length=322, Percent_Identity=25.1552795031056, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24638644, Length=386, Percent_Identity=25.1295336787565, Blast_Score=121, Evalue=1e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001135
- InterPro:   IPR001268
- InterPro:   IPR014029
- InterPro:   IPR001501
- ProDom:   PD001581 [H]

Pfam domain/function: PF00329 Complex1_30kDa; PF00346 Complex1_49kDa; PF00374 NiFeSe_Hases [H]

EC number: NA

Molecular weight: Translated: 58735; Mature: 58735

Theoretical pI: Translated: 7.52; Mature: 7.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSTVSSIAQEKLEILSTRENEIYLRISETGFKEVCPALAERKFSLIGLFCAEAFEGKDN
CCCHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
FTLFYAFKKGGMSPVLILVRETGNEKRITSIAETFPSASWFEREVRDGFGIEFDGAFDTR
EEEEEEEECCCCCEEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHCCCCEEECCCCCHH
RLFLHECYPEGFHPLLKSFKNGPISPVEAPHKEYKFRQHKGEGVYQIPVGPVHAGIIEPG
HHHHHHHCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCEEECCCCCCCCCCCCCC
HFRFSVIGEPIFSLEIRLFYKHRGIEKLAEGKNPSECVALAEAVSGDESMANAAGFCMAV
CEEEEEECCCEEEEEEEEEEHHCCHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHH
EQVCGIKVPERAERLRAVMLELERIYSLLGDLAGMAVDVGFALVASPFFILREEVFRLNE
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLTGSRFLRGITFPGGLKKDIPESALKEIPEFLGKFSRSFESAYNRATSSSSLIDRFVTT
HCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
GVIKKELISPLNLTGPIARASGSSSDTRLDRPYGAYRNFTPEHCLRKKGDVFSRFEIKAS
HHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCHHCCCCHHHHHHCCCCCHHHEECCHH
EIRAAVGLILRLTEKLPQGPVLAENSGSGESAGEINISGTTGYSLSLVEAPRGQNLHWVY
HHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCEEEECCCCCCEEEEEECCCCCCEEEEE
LKNGIVDRYKVRTASFCNWQAIEHAVLGNIVPDFPLINKSLNLSYAGTDL
EECCCCCHHEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCC
>Mature Secondary Structure
MLSTVSSIAQEKLEILSTRENEIYLRISETGFKEVCPALAERKFSLIGLFCAEAFEGKDN
CCCHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
FTLFYAFKKGGMSPVLILVRETGNEKRITSIAETFPSASWFEREVRDGFGIEFDGAFDTR
EEEEEEEECCCCCEEEEEEECCCCCHHHHHHHHHCCCHHHHHHHHHCCCCEEECCCCCHH
RLFLHECYPEGFHPLLKSFKNGPISPVEAPHKEYKFRQHKGEGVYQIPVGPVHAGIIEPG
HHHHHHHCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCCEEECCCCCCCCCCCCCC
HFRFSVIGEPIFSLEIRLFYKHRGIEKLAEGKNPSECVALAEAVSGDESMANAAGFCMAV
CEEEEEECCCEEEEEEEEEEHHCCHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHH
EQVCGIKVPERAERLRAVMLELERIYSLLGDLAGMAVDVGFALVASPFFILREEVFRLNE
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KLTGSRFLRGITFPGGLKKDIPESALKEIPEFLGKFSRSFESAYNRATSSSSLIDRFVTT
HCCHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
GVIKKELISPLNLTGPIARASGSSSDTRLDRPYGAYRNFTPEHCLRKKGDVFSRFEIKAS
HHHHHHHCCCCCCCCCHHHCCCCCCCCCCCCCCCHHCCCCHHHHHHCCCCCHHHEECCHH
EIRAAVGLILRLTEKLPQGPVLAENSGSGESAGEINISGTTGYSLSLVEAPRGQNLHWVY
HHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCCEEEECCCCCCEEEEEECCCCCCEEEEE
LKNGIVDRYKVRTASFCNWQAIEHAVLGNIVPDFPLINKSLNLSYAGTDL
EECCCCCHHEECCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; Proton; reduced ferredoxin [C]

Specific reaction: formate = CO2 + H2 Proton + reduced ferredoxin = H2 + oxidized ferredoxin [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2187144; 9278503; 8125094 [H]