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The map label for this gene is hyfF [H]

Identifier: 21227164

GI number: 21227164

Start: 1245822

End: 1247360

Strand: Direct

Name: hyfF [H]

Synonym: MM_1062

Alternate gene names: 21227164

Gene position: 1245822-1247360 (Clockwise)

Preceding gene: 21227163

Following gene: 21227165

Centisome position: 30.41

GC content: 49.9

Gene sequence:

>1539_bases
GTGATCCGGTACTACCTTCTAATAATGGGAATATTCCTGGCATTCGGCCTTATTCCGAAATCTCACAGGCTTATGAATGC
ACTTGCCGCAGGAAACGGAATGCTGCACCTTGTTGTGAGCTGCGTGGCCCTTTCCGTTCTCGACCTGCCGGGAAGGCTGG
GAGAGTCGCTGTATCTTGACCACCTCAACCTTTATGTAGAACTGATAACCGCACTTGGATTCACTGCTGCAGCCTTCTAT
GCCAGAGGGTACACTGACAGCCTTATGGAAGCAGGAGAACTTGACCCCAAGAACCTGAAGACCTTCTACCTTGCCTTCAA
CCTGCTTCTTATTACAGCCACAGTTGCGCTCTTCTCAGACAACCTGGCACTCTTCTGGATCTTCGCAGAACTCACTACGG
TCTGTGCCGCCATGCTAGTTGCGATACTTTCTGCAAGGGAAAATATCGATGCAGCCTTAAAGTACGTCTTTATAGTTTCC
GGAGCCATGCTCTTTGCTTTCCTGGGATTAATTTTCCTGTTCACACTCTCGGAACAGGCACTTGGGGAAGGCACTCTCAA
CTGGACAGAACTGGCAGCTCATGTTTCAGAATTTTCACCTGCCCTGCTCCTCGCGAGCTTCACATTCATGTTCATAGGTT
TCGGGGCAAAAGCAGGGGTAGCTCCTTTTCACACCTGGCTCCCGGATGCCTATGCAAGAGCACCTTCGGTGAGCGCAATC
CTCTCAGGGCTGATGCAGAACCTGGGGATCTACGGCATAATAAGGATGTATGCCCTGCTCAGGGAAAGCGGCGCAGCCCC
GCAGGCTTCCTCTATCCTTCTTGCAGCAGGGGTTTTCTCAATTGCACTTGCTTCATTCAGCATGTTCCAGCAGAGAAACC
TTAAAAGGCTGATAGCTTTCTCGAGTGTCGAGCATATGGGCCTTCTCCTTATAGGGATAGGAATAGGGACGCCTCTGGCT
CTTTTCTGGGCCCTTTTCTACATGCTCGTCCATGTCCTTGTAAAGGAAACCCTGCTGTTTTCCGCAGGCATCCTGCACAG
GCAGTACAGAAGCAATATGCGTGAAAACATGTGGGATGTTTTTAAACTCCAGCCCAGGGCAGCCTGGAGCCTGATACTTG
GAAGCATTGCGATCGTGGGAATGCCGCCTTTTCCCCTTTTCCCGGCAAAGTTTTTCATCCTGCTGGAAAGTGCGGCAATT
TCCCCTTACCTGACCCTTGCAATACTGCTTATGCTCATCCTTGCAGCTGCCTCCTTTGCAAACTTCCTGTTAAAAGCTTT
CTCAAAAGTAACCCTGGAGGAAGAAATAGAAGGGCAGATTACCAGGCCCCATTCCTTAACAGGAGAGGCTGAACTGAAAA
TCGCAGCCGGAGAAGCAGATCATGATGAAATAAGGCTTCCACGTAAGTATGCTGTTAAGAGCGGGATGAGGATTCCGGCA
GTTTTTATGCTGGCTCTTGCCCTTGCCCTGGGATTTTATTTTCCGGACTCCCTGAAGGATCTGCTTGATGCGATAATTCT
CGAACTGGGATATGTTTAA

Upstream 100 bases:

>100_bases
TCATACTGACCACAATCCTGGCTGTGGGAATGGACTCCAGCATTGAAGAATACAGGCAAAAGTTCCGGAAACTGCAGGTA
TGGGCAGGAGGTGAATCCCA

Downstream 100 bases:

>100_bases
GGGGCGTATGTATGGACATCAAAATGAACGAAGATATAACAGAATTGCTTTCTACAGTCTCCAGCATCGCACAGGAGAAG
CTTGAAATTCTCAGCACCCG

Product: hydrogenase membrane subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 512; Mature: 512

Protein sequence:

>512_residues
MIRYYLLIMGIFLAFGLIPKSHRLMNALAAGNGMLHLVVSCVALSVLDLPGRLGESLYLDHLNLYVELITALGFTAAAFY
ARGYTDSLMEAGELDPKNLKTFYLAFNLLLITATVALFSDNLALFWIFAELTTVCAAMLVAILSARENIDAALKYVFIVS
GAMLFAFLGLIFLFTLSEQALGEGTLNWTELAAHVSEFSPALLLASFTFMFIGFGAKAGVAPFHTWLPDAYARAPSVSAI
LSGLMQNLGIYGIIRMYALLRESGAAPQASSILLAAGVFSIALASFSMFQQRNLKRLIAFSSVEHMGLLLIGIGIGTPLA
LFWALFYMLVHVLVKETLLFSAGILHRQYRSNMRENMWDVFKLQPRAAWSLILGSIAIVGMPPFPLFPAKFFILLESAAI
SPYLTLAILLMLILAAASFANFLLKAFSKVTLEEEIEGQITRPHSLTGEAELKIAAGEADHDEIRLPRKYAVKSGMRIPA
VFMLALALALGFYFPDSLKDLLDAIILELGYV

Sequences:

>Translated_512_residues
MIRYYLLIMGIFLAFGLIPKSHRLMNALAAGNGMLHLVVSCVALSVLDLPGRLGESLYLDHLNLYVELITALGFTAAAFY
ARGYTDSLMEAGELDPKNLKTFYLAFNLLLITATVALFSDNLALFWIFAELTTVCAAMLVAILSARENIDAALKYVFIVS
GAMLFAFLGLIFLFTLSEQALGEGTLNWTELAAHVSEFSPALLLASFTFMFIGFGAKAGVAPFHTWLPDAYARAPSVSAI
LSGLMQNLGIYGIIRMYALLRESGAAPQASSILLAAGVFSIALASFSMFQQRNLKRLIAFSSVEHMGLLLIGIGIGTPLA
LFWALFYMLVHVLVKETLLFSAGILHRQYRSNMRENMWDVFKLQPRAAWSLILGSIAIVGMPPFPLFPAKFFILLESAAI
SPYLTLAILLMLILAAASFANFLLKAFSKVTLEEEIEGQITRPHSLTGEAELKIAAGEADHDEIRLPRKYAVKSGMRIPA
VFMLALALALGFYFPDSLKDLLDAIILELGYV
>Mature_512_residues
MIRYYLLIMGIFLAFGLIPKSHRLMNALAAGNGMLHLVVSCVALSVLDLPGRLGESLYLDHLNLYVELITALGFTAAAFY
ARGYTDSLMEAGELDPKNLKTFYLAFNLLLITATVALFSDNLALFWIFAELTTVCAAMLVAILSARENIDAALKYVFIVS
GAMLFAFLGLIFLFTLSEQALGEGTLNWTELAAHVSEFSPALLLASFTFMFIGFGAKAGVAPFHTWLPDAYARAPSVSAI
LSGLMQNLGIYGIIRMYALLRESGAAPQASSILLAAGVFSIALASFSMFQQRNLKRLIAFSSVEHMGLLLIGIGIGTPLA
LFWALFYMLVHVLVKETLLFSAGILHRQYRSNMRENMWDVFKLQPRAAWSLILGSIAIVGMPPFPLFPAKFFILLESAAI
SPYLTLAILLMLILAAASFANFLLKAFSKVTLEEEIEGQITRPHSLTGEAELKIAAGEADHDEIRLPRKYAVKSGMRIPA
VFMLALALALGFYFPDSLKDLLDAIILELGYV

Specific function: Unknown

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I subunit 5 family [H]

Homologues:

Organism=Homo sapiens, GI251831117, Length=230, Percent_Identity=30.8695652173913, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1788831, Length=342, Percent_Identity=35.0877192982456, Blast_Score=200, Evalue=2e-52,
Organism=Escherichia coli, GI1788827, Length=350, Percent_Identity=28, Blast_Score=121, Evalue=1e-28,
Organism=Escherichia coli, GI1788613, Length=403, Percent_Identity=31.2655086848635, Blast_Score=109, Evalue=5e-25,
Organism=Escherichia coli, GI1788614, Length=356, Percent_Identity=27.247191011236, Blast_Score=82, Evalue=7e-17,
Organism=Escherichia coli, GI1788829, Length=371, Percent_Identity=26.4150943396226, Blast_Score=79, Evalue=7e-16,
Organism=Escherichia coli, GI145693160, Length=174, Percent_Identity=28.735632183908, Blast_Score=76, Evalue=5e-15,
Organism=Escherichia coli, GI2367154, Length=374, Percent_Identity=25.9358288770053, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001750
- InterPro:   IPR003918 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 56029; Mature: 56029

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRYYLLIMGIFLAFGLIPKSHRLMNALAAGNGMLHLVVSCVALSVLDLPGRLGESLYLD
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHCCHHHHH
HLNLYVELITALGFTAAAFYARGYTDSLMEAGELDPKNLKTFYLAFNLLLITATVALFSD
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
NLALFWIFAELTTVCAAMLVAILSARENIDAALKYVFIVSGAMLFAFLGLIFLFTLSEQA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGEGTLNWTELAAHVSEFSPALLLASFTFMFIGFGAKAGVAPFHTWLPDAYARAPSVSAI
HCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHCCCCHHHH
LSGLMQNLGIYGIIRMYALLRESGAAPQASSILLAAGVFSIALASFSMFQQRNLKRLIAF
HHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSVEHMGLLLIGIGIGTPLALFWALFYMLVHVLVKETLLFSAGILHRQYRSNMRENMWDV
HHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FKLQPRAAWSLILGSIAIVGMPPFPLFPAKFFILLESAAISPYLTLAILLMLILAAASFA
HHCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
NFLLKAFSKVTLEEEIEGQITRPHSLTGEAELKIAAGEADHDEIRLPRKYAVKSGMRIPA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHCCCCHHHHHHCCCCCHH
VFMLALALALGFYFPDSLKDLLDAIILELGYV
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIRYYLLIMGIFLAFGLIPKSHRLMNALAAGNGMLHLVVSCVALSVLDLPGRLGESLYLD
CHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCHHHCCHHHHH
HLNLYVELITALGFTAAAFYARGYTDSLMEAGELDPKNLKTFYLAFNLLLITATVALFSD
HHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCC
NLALFWIFAELTTVCAAMLVAILSARENIDAALKYVFIVSGAMLFAFLGLIFLFTLSEQA
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGEGTLNWTELAAHVSEFSPALLLASFTFMFIGFGAKAGVAPFHTWLPDAYARAPSVSAI
HCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCCHHHHCCHHHHCCCCHHHH
LSGLMQNLGIYGIIRMYALLRESGAAPQASSILLAAGVFSIALASFSMFQQRNLKRLIAF
HHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SSVEHMGLLLIGIGIGTPLALFWALFYMLVHVLVKETLLFSAGILHRQYRSNMRENMWDV
HHHHHHHHHHEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FKLQPRAAWSLILGSIAIVGMPPFPLFPAKFFILLESAAISPYLTLAILLMLILAAASFA
HHCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
NFLLKAFSKVTLEEEIEGQITRPHSLTGEAELKIAAGEADHDEIRLPRKYAVKSGMRIPA
HHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEECCCCCCHHCCCCHHHHHHCCCCCHH
VFMLALALALGFYFPDSLKDLLDAIILELGYV
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA