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The map label for this gene is leuB [H]

Identifier: 21227105

GI number: 21227105

Start: 1177056

End: 1178084

Strand: Reverse

Name: leuB [H]

Synonym: MM_1003

Alternate gene names: 21227105

Gene position: 1178084-1177056 (Counterclockwise)

Preceding gene: 21227107

Following gene: 21227103

Centisome position: 28.76

GC content: 48.98

Gene sequence:

>1029_bases
ATGACCAAAACCGCAGCAGTAATCAAAGGTGACGGGGTAGGCCCCGAACTGGTGGATGCAATGCTTAAAGTTGCAGAAGC
CTCTGGCACTGATGTCGAATTCGTAATGTGTGAAGCAGGAGCCGGCTGGTGGGAGGAGCATGGAGGTAATTCCCTTATTC
CTGAAGAGACCTGGCAGATCCTTGACAGTTCTGACGCATGTTTCAAGGGTCCTACAACCACTCCCGGAGGAATCGGCTCC
CCGAGAAGTGTGGCTGTATCCATCAGGAGAAAGTACGACCTTTATGCCAATGTCAGGCCAATAAAAACCTTCCCGAATTC
AAGTGCACCTCTCGGAGATGTCGAAATGGTTTGTGTTCGTGAAGGGACAGAAGGTCTCTACATAGGAGAAGAAATCCAGC
TCACAGACGATGTATCCATTGCAATCAGGAAAATTACCCGGACGGCATCCAGCAAGATTGCCCGCTATGCTTTTGAAGAC
GCAAAAAAGAGAGGTTACGACACAGTTGTGCCAATCCACAAAAGTAACATCCTGAAATTGACCTGCGGCTCATTCCTTGA
AGAGGTCGAAAAGGTTGCTCACGACTTCCCGGATATCGAGGTCTGGCCCTACCATATTGATAATATTGCCCAGCAGCTTA
TCAAGAACCCCCAGATCTTTAACAAGAAAGTCCTCCTTTCCACCAACCTCTTCATGGACGTGATCAGTGAGGAATGCTCA
GCCCTTGTTGGCAGTATCGGGCTTATCTATTCCGCAAATATCGGGGACAACTTTGCAATGTTTGAACCTGCTCACGGCTC
AGCCCCGAAGTATGCAGGCCTTGACAAGGTAAACCCTGTTGCAACCGTTCTTGCGGGCGCCTGGATGCTGGACTACCTTG
GAGAAAAGGACAAATCCGACGCGATCTTTAAAGCCACTGAGCGTGTTATTTCCGAAGGCAAATACGTGACTTATGACCTC
GGAGGAAGCGCAAAGCTCAGCCAGATGGCAGATGAGATCGCAAAATACACAGAAGAAATCCTGAAATAA

Upstream 100 bases:

>100_bases
GATTCAATTATACTTATTAAATTCTTAGAAAAGTTCCCCCTTTTTTAGCTTTTCATCGCTACGGTTCTTTTAACACGAAC
CCTATAAATCTGGTGATAAC

Downstream 100 bases:

>100_bases
AGATTTGAAAATCAATAATTCGAAAATCCGGGGATTCTCGGAGCACAATAAGTGGTAAAACTGATAATAAGAGATTAATT
TGTGTAAGGTTAAATAAGGT

Product: isocitrate dehydrogenase [NADP]

Products: NA

Alternate protein names: 3-IPM-DH; IMDH; Beta-IPM dehydrogenase [H]

Number of amino acids: Translated: 342; Mature: 341

Protein sequence:

>342_residues
MTKTAAVIKGDGVGPELVDAMLKVAEASGTDVEFVMCEAGAGWWEEHGGNSLIPEETWQILDSSDACFKGPTTTPGGIGS
PRSVAVSIRRKYDLYANVRPIKTFPNSSAPLGDVEMVCVREGTEGLYIGEEIQLTDDVSIAIRKITRTASSKIARYAFED
AKKRGYDTVVPIHKSNILKLTCGSFLEEVEKVAHDFPDIEVWPYHIDNIAQQLIKNPQIFNKKVLLSTNLFMDVISEECS
ALVGSIGLIYSANIGDNFAMFEPAHGSAPKYAGLDKVNPVATVLAGAWMLDYLGEKDKSDAIFKATERVISEGKYVTYDL
GGSAKLSQMADEIAKYTEEILK

Sequences:

>Translated_342_residues
MTKTAAVIKGDGVGPELVDAMLKVAEASGTDVEFVMCEAGAGWWEEHGGNSLIPEETWQILDSSDACFKGPTTTPGGIGS
PRSVAVSIRRKYDLYANVRPIKTFPNSSAPLGDVEMVCVREGTEGLYIGEEIQLTDDVSIAIRKITRTASSKIARYAFED
AKKRGYDTVVPIHKSNILKLTCGSFLEEVEKVAHDFPDIEVWPYHIDNIAQQLIKNPQIFNKKVLLSTNLFMDVISEECS
ALVGSIGLIYSANIGDNFAMFEPAHGSAPKYAGLDKVNPVATVLAGAWMLDYLGEKDKSDAIFKATERVISEGKYVTYDL
GGSAKLSQMADEIAKYTEEILK
>Mature_341_residues
TKTAAVIKGDGVGPELVDAMLKVAEASGTDVEFVMCEAGAGWWEEHGGNSLIPEETWQILDSSDACFKGPTTTPGGIGSP
RSVAVSIRRKYDLYANVRPIKTFPNSSAPLGDVEMVCVREGTEGLYIGEEIQLTDDVSIAIRKITRTASSKIARYAFEDA
KKRGYDTVVPIHKSNILKLTCGSFLEEVEKVAHDFPDIEVWPYHIDNIAQQLIKNPQIFNKKVLLSTNLFMDVISEECSA
LVGSIGLIYSANIGDNFAMFEPAHGSAPKYAGLDKVNPVATVLAGAWMLDYLGEKDKSDAIFKATERVISEGKYVTYDLG
GSAKLSQMADEIAKYTEEILK

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=347, Percent_Identity=36.0230547550432, Blast_Score=223, Evalue=1e-58,
Organism=Homo sapiens, GI4758582, Length=339, Percent_Identity=31.2684365781711, Blast_Score=155, Evalue=6e-38,
Organism=Homo sapiens, GI28178816, Length=327, Percent_Identity=33.9449541284404, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI28178821, Length=325, Percent_Identity=33.8461538461538, Blast_Score=149, Evalue=3e-36,
Organism=Homo sapiens, GI28178838, Length=304, Percent_Identity=32.8947368421053, Blast_Score=147, Evalue=1e-35,
Organism=Homo sapiens, GI28178819, Length=183, Percent_Identity=35.5191256830601, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1788101, Length=360, Percent_Identity=33.8888888888889, Blast_Score=179, Evalue=3e-46,
Organism=Escherichia coli, GI87081683, Length=361, Percent_Identity=34.0720221606648, Blast_Score=165, Evalue=4e-42,
Organism=Escherichia coli, GI1787381, Length=366, Percent_Identity=30.6010928961749, Blast_Score=155, Evalue=4e-39,
Organism=Caenorhabditis elegans, GI71986051, Length=338, Percent_Identity=33.1360946745562, Blast_Score=201, Evalue=7e-52,
Organism=Caenorhabditis elegans, GI25144293, Length=342, Percent_Identity=32.1637426900585, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17550882, Length=335, Percent_Identity=32.5373134328358, Blast_Score=160, Evalue=9e-40,
Organism=Caenorhabditis elegans, GI17505779, Length=332, Percent_Identity=31.3253012048193, Blast_Score=156, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6324709, Length=334, Percent_Identity=35.3293413173653, Blast_Score=203, Evalue=3e-53,
Organism=Saccharomyces cerevisiae, GI6322097, Length=352, Percent_Identity=35.2272727272727, Blast_Score=180, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6324291, Length=336, Percent_Identity=30.6547619047619, Blast_Score=168, Evalue=1e-42,
Organism=Saccharomyces cerevisiae, GI6319830, Length=372, Percent_Identity=30.6451612903226, Blast_Score=122, Evalue=6e-29,
Organism=Drosophila melanogaster, GI24643270, Length=332, Percent_Identity=37.0481927710843, Blast_Score=225, Evalue=4e-59,
Organism=Drosophila melanogaster, GI24643268, Length=332, Percent_Identity=37.0481927710843, Blast_Score=225, Evalue=4e-59,
Organism=Drosophila melanogaster, GI24661184, Length=339, Percent_Identity=33.3333333333333, Blast_Score=197, Evalue=6e-51,
Organism=Drosophila melanogaster, GI161078635, Length=330, Percent_Identity=30.6060606060606, Blast_Score=158, Evalue=5e-39,
Organism=Drosophila melanogaster, GI161078637, Length=330, Percent_Identity=30.6060606060606, Blast_Score=158, Evalue=6e-39,
Organism=Drosophila melanogaster, GI161078639, Length=330, Percent_Identity=30.6060606060606, Blast_Score=158, Evalue=6e-39,
Organism=Drosophila melanogaster, GI161078633, Length=330, Percent_Identity=30.6060606060606, Blast_Score=158, Evalue=6e-39,
Organism=Drosophila melanogaster, GI24650122, Length=330, Percent_Identity=30.6060606060606, Blast_Score=158, Evalue=6e-39,
Organism=Drosophila melanogaster, GI281362242, Length=324, Percent_Identity=30.5555555555556, Blast_Score=143, Evalue=1e-34,
Organism=Drosophila melanogaster, GI24648872, Length=324, Percent_Identity=30.5555555555556, Blast_Score=143, Evalue=1e-34,
Organism=Drosophila melanogaster, GI20130355, Length=339, Percent_Identity=24.188790560472, Blast_Score=100, Evalue=2e-21,
Organism=Drosophila melanogaster, GI45550436, Length=242, Percent_Identity=24.3801652892562, Blast_Score=72, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR011828 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 37274; Mature: 37143

Theoretical pI: Translated: 4.67; Mature: 4.67

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKTAAVIKGDGVGPELVDAMLKVAEASGTDVEFVMCEAGAGWWEEHGGNSLIPEETWQI
CCCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHCCCCCCCCHHHHHH
LDSSDACFKGPTTTPGGIGSPRSVAVSIRRKYDLYANVRPIKTFPNSSAPLGDVEMVCVR
HCCCCCCCCCCCCCCCCCCCCHHEEEEEEHHHHHEECCEEEEECCCCCCCCCCEEEEEEE
EGTEGLYIGEEIQLTDDVSIAIRKITRTASSKIARYAFEDAKKRGYDTVVPIHKSNILKL
CCCCCEEECCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEE
TCGSFLEEVEKVAHDFPDIEVWPYHIDNIAQQLIKNPQIFNKKVLLSTNLFMDVISEECS
HHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHCCCCHHCCEEEEEHHHHHHHHHHHHH
ALVGSIGLIYSANIGDNFAMFEPAHGSAPKYAGLDKVNPVATVLAGAWMLDYLGEKDKSD
HHHHCHHEEEECCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
AIFKATERVISEGKYVTYDLGGSAKLSQMADEIAKYTEEILK
HHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
TKTAAVIKGDGVGPELVDAMLKVAEASGTDVEFVMCEAGAGWWEEHGGNSLIPEETWQI
CCCEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEEEECCCCHHHHCCCCCCCCHHHHHH
LDSSDACFKGPTTTPGGIGSPRSVAVSIRRKYDLYANVRPIKTFPNSSAPLGDVEMVCVR
HCCCCCCCCCCCCCCCCCCCCHHEEEEEEHHHHHEECCEEEEECCCCCCCCCCEEEEEEE
EGTEGLYIGEEIQLTDDVSIAIRKITRTASSKIARYAFEDAKKRGYDTVVPIHKSNILKL
CCCCCEEECCEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEE
TCGSFLEEVEKVAHDFPDIEVWPYHIDNIAQQLIKNPQIFNKKVLLSTNLFMDVISEECS
HHHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHCCCCHHCCEEEEEHHHHHHHHHHHHH
ALVGSIGLIYSANIGDNFAMFEPAHGSAPKYAGLDKVNPVATVLAGAWMLDYLGEKDKSD
HHHHCHHEEEECCCCCCEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCC
AIFKATERVISEGKYVTYDLGGSAKLSQMADEIAKYTEEILK
HHHHHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]