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The map label for this gene is purF [C]

Identifier: 21227068

GI number: 21227068

Start: 1144225

End: 1145322

Strand: Direct

Name: purF [C]

Synonym: MM_0966

Alternate gene names: 21227068

Gene position: 1144225-1145322 (Clockwise)

Preceding gene: 21227067

Following gene: 21227069

Centisome position: 27.93

GC content: 46.72

Gene sequence:

>1098_bases
TTGCTCTTATCACGCTCATATCTATCCATTTCTAGAGTGATCAAAATGTGTGGAATAATAGGCTTTATAGATCGAACAAA
GTCCAGAATGGACGGGTCGGGCATAAAAGCCGCTCTTAGTCTCATGAATGAAAGAGGCAGTGGAGACGGGGCAGGCTATG
CTGCATACGGGATTTACCCCGAATATGCGGACTACTATGCTCTTCATGTTTTCTTTGATAACCTGGCTGAACCAAAAAAG
AAGGTTGATGAACTCCTCCAGCAGTGGGGAGAAATAGTTCACCAGGAAGAAATTCCGACTACCCCTCAACCAGGAATCAG
AAAGGTCCATATCCCCTGGAGATATTTCTTCAAACCCTCGGAAGACCTGATGGCAGGAAGGATGAACTCTGAAAATGATG
TTGTAACGTACATAGTCATGGAAGTAAACGCCAATGTAAAAGGCGCCACAATATTTTCCTCCGGCAAAAATATGGGGGTT
TTCAAAGCTTCAGGCTGGCCTGAGGATGTAGCAAACTTCTACAGGATTGAAGACTACAAGGGCTATATCTGGCTTGCTCA
CAACCGCTACCCAACCAACTCTCCGGGCTGGTGGGGAGGAGCTCACCCTTTTAACCTGCTTAACTGGTCAGTAGTGCACA
ATGGAGAAATTACCTCATACGGCACTAACCAGCGCTATGTCGAGGGGTACGGATACAAATGTACCCTCTTTACCGATACC
GAAGTTGTAGCTTACCTTTTCGACCTTCTCGGAAGGCAGCACGAACTCTCCCATGAAATGATCGTCAGGGCTCTTGCCCC
TCCGTTCTGGGACGACATAGACAGAATGCCGGAAAAGGAAGCTGAATTAAATAAGGCAGTCCGCCTGACCTATGGGTCTG
CCCTTATGAACGGTCCTTTTGCTATTGTTGTCGGCACTGATGAAGGAATTGTAGGATTTACTGACAGAATTAAGCTTCGC
CCCCTCGTAGTTGGAGAAAACGGAAGCAAACTTTACATCTCCAGTGAAGAAGCAGCAATAAGGACCCTTGACCCCGAAGT
AAAGAGTATTTACACTCCCAGGGCAGGAGAACCCATAATAGGGAGGTTTATCGAATGA

Upstream 100 bases:

>100_bases
AGAACCCAGTTATCCAGCTGGCAAATTTTTTGAACTATTTCTCAAAAATGAGCCGCATAAGTCCTGGGAAATACCCGGAA
ACGAAGGGATTATTAGAAAA

Downstream 100 bases:

>100_bases
CGCTCGGAAGTGTACCCCCAAAATTTAAGGTCAGCATTGACCGCGACCAGTGCATGGACTGCGGCCGGTGCATTGAAAAC
TGTTCCTACGGCGTATACAG

Product: glutamate synthase, large chain

Products: L-Glutamate; Diphosphate; 5-Phospho-beta-D-ribosylamine [C]

Alternate protein names: NA

Number of amino acids: Translated: 365; Mature: 365

Protein sequence:

>365_residues
MLLSRSYLSISRVIKMCGIIGFIDRTKSRMDGSGIKAALSLMNERGSGDGAGYAAYGIYPEYADYYALHVFFDNLAEPKK
KVDELLQQWGEIVHQEEIPTTPQPGIRKVHIPWRYFFKPSEDLMAGRMNSENDVVTYIVMEVNANVKGATIFSSGKNMGV
FKASGWPEDVANFYRIEDYKGYIWLAHNRYPTNSPGWWGGAHPFNLLNWSVVHNGEITSYGTNQRYVEGYGYKCTLFTDT
EVVAYLFDLLGRQHELSHEMIVRALAPPFWDDIDRMPEKEAELNKAVRLTYGSALMNGPFAIVVGTDEGIVGFTDRIKLR
PLVVGENGSKLYISSEEAAIRTLDPEVKSIYTPRAGEPIIGRFIE

Sequences:

>Translated_365_residues
MLLSRSYLSISRVIKMCGIIGFIDRTKSRMDGSGIKAALSLMNERGSGDGAGYAAYGIYPEYADYYALHVFFDNLAEPKK
KVDELLQQWGEIVHQEEIPTTPQPGIRKVHIPWRYFFKPSEDLMAGRMNSENDVVTYIVMEVNANVKGATIFSSGKNMGV
FKASGWPEDVANFYRIEDYKGYIWLAHNRYPTNSPGWWGGAHPFNLLNWSVVHNGEITSYGTNQRYVEGYGYKCTLFTDT
EVVAYLFDLLGRQHELSHEMIVRALAPPFWDDIDRMPEKEAELNKAVRLTYGSALMNGPFAIVVGTDEGIVGFTDRIKLR
PLVVGENGSKLYISSEEAAIRTLDPEVKSIYTPRAGEPIIGRFIE
>Mature_365_residues
MLLSRSYLSISRVIKMCGIIGFIDRTKSRMDGSGIKAALSLMNERGSGDGAGYAAYGIYPEYADYYALHVFFDNLAEPKK
KVDELLQQWGEIVHQEEIPTTPQPGIRKVHIPWRYFFKPSEDLMAGRMNSENDVVTYIVMEVNANVKGATIFSSGKNMGV
FKASGWPEDVANFYRIEDYKGYIWLAHNRYPTNSPGWWGGAHPFNLLNWSVVHNGEITSYGTNQRYVEGYGYKCTLFTDT
EVVAYLFDLLGRQHELSHEMIVRALAPPFWDDIDRMPEKEAELNKAVRLTYGSALMNGPFAIVVGTDEGIVGFTDRIKLR
PLVVGENGSKLYISSEEAAIRTLDPEVKSIYTPRAGEPIIGRFIE

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0067

COG function: function code E; Glutamate synthase domain 1

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR012375 [H]

Pfam domain/function: PF00310 GATase_2 [H]

EC number: 2.4.2.14 [C]

Molecular weight: Translated: 41157; Mature: 41157

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLSRSYLSISRVIKMCGIIGFIDRTKSRMDGSGIKAALSLMNERGSGDGAGYAAYGIYP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCEEEECCCC
EYADYYALHVFFDNLAEPKKKVDELLQQWGEIVHQEEIPTTPQPGIRKVHIPWRYFFKPS
CHHHHEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEECCC
EDLMAGRMNSENDVVTYIVMEVNANVKGATIFSSGKNMGVFKASGWPEDVANFYRIEDYK
HHHHHCCCCCCCCEEEEEEEEECCCCCEEEEEECCCCCEEEECCCCHHHHHHHEEEECCC
GYIWLAHNRYPTNSPGWWGGAHPFNLLNWSVVHNGEITSYGTNQRYVEGYGYKCTLFTDT
CEEEEEECCCCCCCCCCCCCCCCCCEEEEEEEECCEEEEECCCCCEEECCCEEEEEEECH
EVVAYLFDLLGRQHELSHEMIVRALAPPFWDDIDRMPEKEAELNKAVRLTYGSALMNGPF
HHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHEEEEEECHHHHCCCE
AIVVGTDEGIVGFTDRIKLRPLVVGENGSKLYISSEEAAIRTLDPEVKSIYTPRAGEPII
EEEEECCCCEEEECCCEEEEEEEECCCCCEEEEECCCCEEEECCCHHHHEECCCCCCCHH
GRFIE
HHCCC
>Mature Secondary Structure
MLLSRSYLSISRVIKMCGIIGFIDRTKSRMDGSGIKAALSLMNERGSGDGAGYAAYGIYP
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCCEEEECCCC
EYADYYALHVFFDNLAEPKKKVDELLQQWGEIVHQEEIPTTPQPGIRKVHIPWRYFFKPS
CHHHHEEEEEHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEECCEEEEECCC
EDLMAGRMNSENDVVTYIVMEVNANVKGATIFSSGKNMGVFKASGWPEDVANFYRIEDYK
HHHHHCCCCCCCCEEEEEEEEECCCCCEEEEEECCCCCEEEECCCCHHHHHHHEEEECCC
GYIWLAHNRYPTNSPGWWGGAHPFNLLNWSVVHNGEITSYGTNQRYVEGYGYKCTLFTDT
CEEEEEECCCCCCCCCCCCCCCCCCEEEEEEEECCEEEEECCCCCEEECCCEEEEEEECH
EVVAYLFDLLGRQHELSHEMIVRALAPPFWDDIDRMPEKEAELNKAVRLTYGSALMNGPF
HHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHHHHEEEEEECHHHHCCCE
AIVVGTDEGIVGFTDRIKLRPLVVGENGSKLYISSEEAAIRTLDPEVKSIYTPRAGEPII
EEEEECCCCEEEECCCEEEEEEEECCCCCEEEEECCCCEEEECCCHHHHEECCCCCCCHH
GRFIE
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Mg-phosphoribosyldiphosphate Mg-phosphoribosyldiphosphate, [C]

Metal ions: Mg2+ [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 8.8 {NH3}} 0.067 {5-phospho-alpha-D-ribose} 1.7 {glutamine}} [C]

Substrates: L-Glutamine; H2O; 5-Phospho-alpha-D-ribose 1-diphosphate [C]

Specific reaction: L-Glutamine + H2O + 5-Phospho-alpha-D-ribose 1-diphosphate --> L-Glutamate + Diphosphate + 5-Phospho-beta-D-ribosylamine [C]

General reaction: Pentosyl group transfer [C]

Inhibitor: 1, 10-Phenanthroline; 2-Mercaptoethanol; 6-Diazo-5-oxo-L-norleucine analogue [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]