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The map label for this gene is gltB [C]

Identifier: 21227069

GI number: 21227069

Start: 1145319

End: 1146830

Strand: Direct

Name: gltB [C]

Synonym: MM_0967

Alternate gene names: 21227069

Gene position: 1145319-1146830 (Clockwise)

Preceding gene: 21227068

Following gene: 21227070

Centisome position: 27.96

GC content: 52.12

Gene sequence:

>1512_bases
ATGACGCTCGGAAGTGTACCCCCAAAATTTAAGGTCAGCATTGACCGCGACCAGTGCATGGACTGCGGCCGGTGCATTGA
AAACTGTTCCTACGGCGTATACAGGAGAGAAGGCGACAAAATCCTGATAGAATCAAGGAAGTGTACTGCCTGTCTCCGCT
GTGTTTCCATGTGCCCCAGGGACGCAATAAGTATTACAGAAAAACCAGTTGACTACCGCAGCCACCCAGTCTGGACAAGG
GAAGCCAGAGAAGATATAATCAATCAGGCACGCAGCGGGAAGATCATCCTTTCAGGGATGGGAAATGCCAGAGACCTTCC
AATTATCTACGACCGCCTTCTGCTCGATGCCTGCCAGGTTACAAACCCGAGCATTGACCCCCTGAGAGAGCCAATGGAGC
TCAGGACCTATATAGGAAAAAAGCCGGCAAAACTCAAATTCAGAAAAACCGAGAGCGGAGATGTCGAGCTGGACACAAAA
CTTGCTCCAAACCTCAAGCTTGATACCCCTATAATGATCGGACACATGAGCTTCGGGGCTATCAGCCTTAACTCCCAGCT
CAGTATGGCAAAAGCCGTAGCAGAGACCGGTACTTTTATGGGGACAGGAGAAGGAGGCCTGCACAAGGATCTCTACCCCT
ACCAGAATCACATGATTGTCCAGGTAGCTTCAGGCCGTTTCGGAGTAAATATCGACTACCTGGAAAGAGGCGCTGCCATT
GAGATCAAGATAGGGCAGGGAGCAAAGCCGGGTATAGGAGGGCACCTCCCCGGAGAAAAGGTAAACGATGAAGTTTCCAG
AACCCGTATGATCCCACTTGGAAGCGATGCAATCAGCCCTGCTCCTCACCACGATATTTACAGTATCGAAGATCTCGTCC
AGCTCATCAGAAGCCTGAAAGAAGCGACCGAATGGAAAAAGCCTGTCTTTGTAAAGATTGCAGCCGTGCACAATGTGGCT
CCTATAGCTGCGGGCATTGCAAGGTCCTCAGCAGATGCAGTGGTCATTGACGGGTTCCGTGGCGGGACCGGGGCAGCCCC
GAAAGTATTCAGGGACCATGTAGGAATCCCCATCGAAGCAGCAATTGCAAGCGTTGACCAGAAACTGAGGGACCAGGGTG
TAAGGAACGAGATTTCCATCATCGCAAGCGGCGGTATAAGAAGCAGCGCTGACCTTGCCAAGTCAATAGCTCTCGGAGCA
GATGCAGTCTATATAGGAACTGCCGCTCTTGTTGCTCTCGGATGCAGAGTATGTGGCAACTGTTACAGAAACCTCTGCCC
ATGGGGAATTGCTACCCAGCGCCCGGACCTTGTAAGCAGGCTTGACCCGGAAGCCGGAGCAGCACAGGTTTCAAACCTTA
TACACGGATGGACCCTGGAACTCAGCGAACTTATGGGTGCAGCAGGCATCAACAGTATAGAGAGCCTGCGCGGAAACAGG
GACCGCCTGCGAGGATATATGCTGGATGAAGGAATGCTTAAAATCCTGGATGTACTGCCAGCGGGGGCCTGA

Upstream 100 bases:

>100_bases
ACATCTCCAGTGAAGAAGCAGCAATAAGGACCCTTGACCCCGAAGTAAAGAGTATTTACACTCCCAGGGCAGGAGAACCC
ATAATAGGGAGGTTTATCGA

Downstream 100 bases:

>100_bases
ACATGAAGACAGTAAGAATTGATGCCAGGTGTATGCACTACACCCCCTTAAACCAAAAAATAAGGGCTGCAGTTGCAGAC
GGGGCAGAAGAGATCATCCT

Product: glutamate synthase, large chain

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 503; Mature: 502

Protein sequence:

>503_residues
MTLGSVPPKFKVSIDRDQCMDCGRCIENCSYGVYRREGDKILIESRKCTACLRCVSMCPRDAISITEKPVDYRSHPVWTR
EAREDIINQARSGKIILSGMGNARDLPIIYDRLLLDACQVTNPSIDPLREPMELRTYIGKKPAKLKFRKTESGDVELDTK
LAPNLKLDTPIMIGHMSFGAISLNSQLSMAKAVAETGTFMGTGEGGLHKDLYPYQNHMIVQVASGRFGVNIDYLERGAAI
EIKIGQGAKPGIGGHLPGEKVNDEVSRTRMIPLGSDAISPAPHHDIYSIEDLVQLIRSLKEATEWKKPVFVKIAAVHNVA
PIAAGIARSSADAVVIDGFRGGTGAAPKVFRDHVGIPIEAAIASVDQKLRDQGVRNEISIIASGGIRSSADLAKSIALGA
DAVYIGTAALVALGCRVCGNCYRNLCPWGIATQRPDLVSRLDPEAGAAQVSNLIHGWTLELSELMGAAGINSIESLRGNR
DRLRGYMLDEGMLKILDVLPAGA

Sequences:

>Translated_503_residues
MTLGSVPPKFKVSIDRDQCMDCGRCIENCSYGVYRREGDKILIESRKCTACLRCVSMCPRDAISITEKPVDYRSHPVWTR
EAREDIINQARSGKIILSGMGNARDLPIIYDRLLLDACQVTNPSIDPLREPMELRTYIGKKPAKLKFRKTESGDVELDTK
LAPNLKLDTPIMIGHMSFGAISLNSQLSMAKAVAETGTFMGTGEGGLHKDLYPYQNHMIVQVASGRFGVNIDYLERGAAI
EIKIGQGAKPGIGGHLPGEKVNDEVSRTRMIPLGSDAISPAPHHDIYSIEDLVQLIRSLKEATEWKKPVFVKIAAVHNVA
PIAAGIARSSADAVVIDGFRGGTGAAPKVFRDHVGIPIEAAIASVDQKLRDQGVRNEISIIASGGIRSSADLAKSIALGA
DAVYIGTAALVALGCRVCGNCYRNLCPWGIATQRPDLVSRLDPEAGAAQVSNLIHGWTLELSELMGAAGINSIESLRGNR
DRLRGYMLDEGMLKILDVLPAGA
>Mature_502_residues
TLGSVPPKFKVSIDRDQCMDCGRCIENCSYGVYRREGDKILIESRKCTACLRCVSMCPRDAISITEKPVDYRSHPVWTRE
AREDIINQARSGKIILSGMGNARDLPIIYDRLLLDACQVTNPSIDPLREPMELRTYIGKKPAKLKFRKTESGDVELDTKL
APNLKLDTPIMIGHMSFGAISLNSQLSMAKAVAETGTFMGTGEGGLHKDLYPYQNHMIVQVASGRFGVNIDYLERGAAIE
IKIGQGAKPGIGGHLPGEKVNDEVSRTRMIPLGSDAISPAPHHDIYSIEDLVQLIRSLKEATEWKKPVFVKIAAVHNVAP
IAAGIARSSADAVVIDGFRGGTGAAPKVFRDHVGIPIEAAIASVDQKLRDQGVRNEISIIASGGIRSSADLAKSIALGAD
AVYIGTAALVALGCRVCGNCYRNLCPWGIATQRPDLVSRLDPEAGAAQVSNLIHGWTLELSELMGAAGINSIESLRGNRD
RLRGYMLDEGMLKILDVLPAGA

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0069

COG function: function code E; Glutamate synthase domain 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI308199519, Length=266, Percent_Identity=40.6015037593985, Blast_Score=186, Evalue=4e-48,
Organism=Caenorhabditis elegans, GI17570289, Length=334, Percent_Identity=38.9221556886228, Blast_Score=208, Evalue=4e-54,
Organism=Saccharomyces cerevisiae, GI6320030, Length=319, Percent_Identity=36.9905956112853, Blast_Score=196, Evalue=5e-51,
Organism=Drosophila melanogaster, GI28574881, Length=334, Percent_Identity=37.125748502994, Blast_Score=191, Evalue=7e-49,
Organism=Drosophila melanogaster, GI24665539, Length=334, Percent_Identity=37.125748502994, Blast_Score=191, Evalue=7e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR013785
- InterPro:   IPR002932 [H]

Pfam domain/function: PF01645 Glu_synthase [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 54321; Mature: 54190

Theoretical pI: Translated: 7.98; Mature: 7.98

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLGSVPPKFKVSIDRDQCMDCGRCIENCSYGVYRREGDKILIESRKCTACLRCVSMCPR
CCCCCCCCCEEEEECHHHHHHHHHHHHHCCCCCEEECCCEEEEECCHHHHHHHHHHHCCC
DAISITEKPVDYRSHPVWTREAREDIINQARSGKIILSGMGNARDLPIIYDRLLLDACQV
CHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHC
TNPSIDPLREPMELRTYIGKKPAKLKFRKTESGDVELDTKLAPNLKLDTPIMIGHMSFGA
CCCCHHHHHCHHHHHHHHCCCCCCEEEEECCCCCEEEECCCCCCCCCCCCEEEEECCCCE
ISLNSQLSMAKAVAETGTFMGTGEGGLHKDLYPYQNHMIVQVASGRFGVNIDYLERGAAI
EECCCHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHCCCCEE
EIKIGQGAKPGIGGHLPGEKVNDEVSRTRMIPLGSDAISPAPHHDIYSIEDLVQLIRSLK
EEEECCCCCCCCCCCCCCCCCCCHHHHEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHH
EATEWKKPVFVKIAAVHNVAPIAAGIARSSADAVVIDGFRGGTGAAPKVFRDHVGIPIEA
HHHHCCCCEEEEEEEHHCHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHCCCCHHH
AIASVDQKLRDQGVRNEISIIASGGIRSSADLAKSIALGADAVYIGTAALVALGCRVCGN
HHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
CYRNLCPWGIATQRPDLVSRLDPEAGAAQVSNLIHGWTLELSELMGAAGINSIESLRGNR
HHHHCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHEEHHHHHCCCCCCHHHHHCCCH
DRLRGYMLDEGMLKILDVLPAGA
HHHCCCHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TLGSVPPKFKVSIDRDQCMDCGRCIENCSYGVYRREGDKILIESRKCTACLRCVSMCPR
CCCCCCCCEEEEECHHHHHHHHHHHHHCCCCCEEECCCEEEEECCHHHHHHHHHHHCCC
DAISITEKPVDYRSHPVWTREAREDIINQARSGKIILSGMGNARDLPIIYDRLLLDACQV
CHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHHHHC
TNPSIDPLREPMELRTYIGKKPAKLKFRKTESGDVELDTKLAPNLKLDTPIMIGHMSFGA
CCCCHHHHHCHHHHHHHHCCCCCCEEEEECCCCCEEEECCCCCCCCCCCCEEEEECCCCE
ISLNSQLSMAKAVAETGTFMGTGEGGLHKDLYPYQNHMIVQVASGRFGVNIDYLERGAAI
EECCCHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCEEEEEECCCCCCCHHHHCCCCEE
EIKIGQGAKPGIGGHLPGEKVNDEVSRTRMIPLGSDAISPAPHHDIYSIEDLVQLIRSLK
EEEECCCCCCCCCCCCCCCCCCCHHHHEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHH
EATEWKKPVFVKIAAVHNVAPIAAGIARSSADAVVIDGFRGGTGAAPKVFRDHVGIPIEA
HHHHCCCCEEEEEEEHHCHHHHHHHHHCCCCCEEEEECCCCCCCCCCHHHHHHCCCCHHH
AIASVDQKLRDQGVRNEISIIASGGIRSSADLAKSIALGADAVYIGTAALVALGCRVCGN
HHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHH
CYRNLCPWGIATQRPDLVSRLDPEAGAAQVSNLIHGWTLELSELMGAAGINSIESLRGNR
HHHHCCCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHEEHHHHHCCCCCCHHHHHCCCH
DRLRGYMLDEGMLKILDVLPAGA
HHHCCCHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]