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The map label for this gene is glnB [H]

Identifier: 21227060

GI number: 21227060

Start: 1138938

End: 1139285

Strand: Direct

Name: glnB [H]

Synonym: MM_0958

Alternate gene names: 21227060

Gene position: 1138938-1139285 (Clockwise)

Preceding gene: 21227059

Following gene: 21227061

Centisome position: 27.8

GC content: 44.54

Gene sequence:

>348_bases
ATGACCAGGATGTGCAAAATAGAAGCGATCATTAAGCCCCTGAAACTGCACCAGGTAAAAGATGCCCTGGAAGAAGCAGG
ATTCGCAAGCCTGACAGTTACCGATGTCAAAGGGCGAGGCCAGCAAAAAGGGATCGTACAGCAGTGGAGAGGAAAAAAAT
ACTGTGTAGACCTTCTTCCAAAAACTAAAATAGAAATAGTTGTGCCGGAAGAAAAGATCGAAGAAATAATACAGGTTATC
CAGAGTACAGCTTATACCGGAGAAATCGGAGACGGAAAAATCTTCGTAATCCCGATAGAAACCGTGGTCAGGATAAGAAC
CGGAGAAAGGAACGGAGATGCCCTGTAA

Upstream 100 bases:

>100_bases
TAGGACTCAGGGTATCCGAAGAAGAAGAAATTATGGGCCTTGATATTTGTGAGCACGGAATAAGAGCGTATCCCGAGTAT
CTGATGAGGGAGGAATAAAC

Downstream 100 bases:

>100_bases
CCAGGCACCTCTTTATTCTCATTTACATTATTTACCATTGAACAAAGAAACGCTTAAAGAATAAGGCAGATAGACGATGA
CCGAACTAAAATCAGAAAGC

Product: nitrogen regulatory protein P-II

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 115; Mature: 114

Protein sequence:

>115_residues
MTRMCKIEAIIKPLKLHQVKDALEEAGFASLTVTDVKGRGQQKGIVQQWRGKKYCVDLLPKTKIEIVVPEEKIEEIIQVI
QSTAYTGEIGDGKIFVIPIETVVRIRTGERNGDAL

Sequences:

>Translated_115_residues
MTRMCKIEAIIKPLKLHQVKDALEEAGFASLTVTDVKGRGQQKGIVQQWRGKKYCVDLLPKTKIEIVVPEEKIEEIIQVI
QSTAYTGEIGDGKIFVIPIETVVRIRTGERNGDAL
>Mature_114_residues
TRMCKIEAIIKPLKLHQVKDALEEAGFASLTVTDVKGRGQQKGIVQQWRGKKYCVDLLPKTKIEIVVPEEKIEEIIQVIQ
STAYTGEIGDGKIFVIPIETVVRIRTGERNGDAL

Specific function: In nitrogen-limiting conditions, when the ratio of Gln to 2-ketoglutarate decreases, P-II is uridylylated to P-II-UMP. P- II-UMP allows the deadenylation of glutamine synthetase (GS), thus activating the enzyme. Conversely, in nitrogen excess P-II is deur

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the P(II) protein family [H]

Homologues:

Organism=Escherichia coli, GI1788904, Length=112, Percent_Identity=54.4642857142857, Blast_Score=121, Evalue=1e-29,
Organism=Escherichia coli, GI1786655, Length=112, Percent_Identity=48.2142857142857, Blast_Score=115, Evalue=7e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002187
- InterPro:   IPR011322
- InterPro:   IPR015867
- InterPro:   IPR017918
- InterPro:   IPR002332 [H]

Pfam domain/function: PF00543 P-II [H]

EC number: NA

Molecular weight: Translated: 12861; Mature: 12730

Theoretical pI: Translated: 8.72; Mature: 8.72

Prosite motif: PS00638 PII_GLNB_CTER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRMCKIEAIIKPLKLHQVKDALEEAGFASLTVTDVKGRGQQKGIVQQWRGKKYCVDLLP
CCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHCCCCEEEECCC
KTKIEIVVPEEKIEEIIQVIQSTAYTGEIGDGKIFVIPIETVVRIRTGERNGDAL
CCEEEEEECHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHEEEEECCCCCCCCC
>Mature Secondary Structure 
TRMCKIEAIIKPLKLHQVKDALEEAGFASLTVTDVKGRGQQKGIVQQWRGKKYCVDLLP
CCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCHHHHHCCCCEEEECCC
KTKIEIVVPEEKIEEIIQVIQSTAYTGEIGDGKIFVIPIETVVRIRTGERNGDAL
CCEEEEEECHHHHHHHHHHHHHCCCCCCCCCCEEEEEEHHHEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1702507; 2878685 [H]