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The map label for this gene is amtB [C]

Identifier: 21227059

GI number: 21227059

Start: 1137526

End: 1138935

Strand: Direct

Name: amtB [C]

Synonym: MM_0957

Alternate gene names: 21227059

Gene position: 1137526-1138935 (Clockwise)

Preceding gene: 21227050

Following gene: 21227060

Centisome position: 27.77

GC content: 48.94

Gene sequence:

>1410_bases
ATGTCAAAAAATGTACAGAAGATAGAAAAACTGCTGATAGTAATCTGTATGATATTTATAGTGCTGCCTTCACCGGCACT
TGCTGTAACGGCAGAAGAAAATGCAGAAGCAATCGAAGGGCTTCAAACTACACTTACATTCTTATGGCTGCTTATAGCAA
GCGGACTGGTATTTTTTATGCATGCGGGTTTCTCGCTGGTAGAAACCGGCCTTACCCGGAGTAAAAACACAGCCAATATC
CTGATGAAAAACTTCATGACTGTAGTTCTCGGCATTCTCGTCTACTGGGCAGTGGGCTGGGGAGTTATGTATGGAGCTGA
TGCGGCAGGACTTATAGGGACTGATCAGTTCTTCCTGGCAGGTGCGGATAACGCAACCTGGAACAGCTGGTTCTTCCAGA
TGGTATTCGCAGCCACAGGCGCAACTATAGTTTCAGGAGCAATGGCTGAGAGGACAAACTTCAAAGCTTACCTTGTTTAC
TGTGTCCTGATGGTAGCAGTAATCTACCCAATATACGGGCACTGGGTATGGAGCGGAGCTGACACGGCTCTCCTGAGCGG
AGCCGATAGCCCGATCGTAAAAGCAATAGGAGTGGCACACCATGACTTTGCAGGTTCCGGAGTGGTGCACTCGATAGGTG
GGTATTCTGCCCTTGCAGGCGTACTCCTTGTAGGTGCAAGGATAGGCAAATTCAGGGATGGAAAAGCCATACCGATTCCA
GGTCACAACCTGACCATTGCTTTCCTCGGAACTCTGATCCTCGCCCTCGGATGGCTGGGGTTCAACGGAGGGAGCACCCT
TAACGCAAACGACCCTTACATGAACCTTGTAGTGGTCAATACTTTCCTGGCAGGTGCTGTTGGAGCTGTCACAACTATGG
CAATTACCTGGATGAAAACCGGAAAACCAGATCCATCTCTTACTGCAAACGGTCTTCTCGGAGGGCTTGTGGCAGTCACT
GCCCCATGTGGCTCGATATCGAACTGGGGAGCCATAGCAATAGGAATCATTGCCGGGATAATCATCTATGCAGGTGTCAT
GTTTAACGAGAACAAACTTAAACTGGACGACCCGGTAGGTGCAATTGCCGTACACGGATACTGTGGAACCTGGGGTCTGA
TCTCTGTCGGGCTCTTCTCGATAGGTATAGGAAACGGCATCCTTGCAGACGCTTCGTATGCCGCCGCGGTTCCCGGACTC
TTCTACGGAGGAGGGGTAAGTTTGCTTCTTATCCAGCTAGTGGCAGTTCTGATAACCATTGTCTGGGGCTTTGGATTATC
ATTTATAATCTTCAAGATCCTCGATGCCGTAATAGGACTCAGGGTATCCGAAGAAGAAGAAATTATGGGCCTTGATATTT
GTGAGCACGGAATAAGAGCGTATCCCGAGTATCTGATGAGGGAGGAATAA

Upstream 100 bases:

>100_bases
TTACACTAACTTGCCGTGCGCCTAATAGAAAATTATAAATAATATTAGATCTACAACCACTTACCGGTTGCCGATGGAAA
AAAAGGAGGCAAAGAACGGT

Downstream 100 bases:

>100_bases
ACATGACCAGGATGTGCAAAATAGAAGCGATCATTAAGCCCCTGAAACTGCACCAGGTAAAAGATGCCCTGGAAGAAGCA
GGATTCGCAAGCCTGACAGT

Product: ammonium transporter

Products: NH3 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MSKNVQKIEKLLIVICMIFIVLPSPALAVTAEENAEAIEGLQTTLTFLWLLIASGLVFFMHAGFSLVETGLTRSKNTANI
LMKNFMTVVLGILVYWAVGWGVMYGADAAGLIGTDQFFLAGADNATWNSWFFQMVFAATGATIVSGAMAERTNFKAYLVY
CVLMVAVIYPIYGHWVWSGADTALLSGADSPIVKAIGVAHHDFAGSGVVHSIGGYSALAGVLLVGARIGKFRDGKAIPIP
GHNLTIAFLGTLILALGWLGFNGGSTLNANDPYMNLVVVNTFLAGAVGAVTTMAITWMKTGKPDPSLTANGLLGGLVAVT
APCGSISNWGAIAIGIIAGIIIYAGVMFNENKLKLDDPVGAIAVHGYCGTWGLISVGLFSIGIGNGILADASYAAAVPGL
FYGGGVSLLLIQLVAVLITIVWGFGLSFIIFKILDAVIGLRVSEEEEIMGLDICEHGIRAYPEYLMREE

Sequences:

>Translated_469_residues
MSKNVQKIEKLLIVICMIFIVLPSPALAVTAEENAEAIEGLQTTLTFLWLLIASGLVFFMHAGFSLVETGLTRSKNTANI
LMKNFMTVVLGILVYWAVGWGVMYGADAAGLIGTDQFFLAGADNATWNSWFFQMVFAATGATIVSGAMAERTNFKAYLVY
CVLMVAVIYPIYGHWVWSGADTALLSGADSPIVKAIGVAHHDFAGSGVVHSIGGYSALAGVLLVGARIGKFRDGKAIPIP
GHNLTIAFLGTLILALGWLGFNGGSTLNANDPYMNLVVVNTFLAGAVGAVTTMAITWMKTGKPDPSLTANGLLGGLVAVT
APCGSISNWGAIAIGIIAGIIIYAGVMFNENKLKLDDPVGAIAVHGYCGTWGLISVGLFSIGIGNGILADASYAAAVPGL
FYGGGVSLLLIQLVAVLITIVWGFGLSFIIFKILDAVIGLRVSEEEEIMGLDICEHGIRAYPEYLMREE
>Mature_468_residues
SKNVQKIEKLLIVICMIFIVLPSPALAVTAEENAEAIEGLQTTLTFLWLLIASGLVFFMHAGFSLVETGLTRSKNTANIL
MKNFMTVVLGILVYWAVGWGVMYGADAAGLIGTDQFFLAGADNATWNSWFFQMVFAATGATIVSGAMAERTNFKAYLVYC
VLMVAVIYPIYGHWVWSGADTALLSGADSPIVKAIGVAHHDFAGSGVVHSIGGYSALAGVLLVGARIGKFRDGKAIPIPG
HNLTIAFLGTLILALGWLGFNGGSTLNANDPYMNLVVVNTFLAGAVGAVTTMAITWMKTGKPDPSLTANGLLGGLVAVTA
PCGSISNWGAIAIGIIAGIIIYAGVMFNENKLKLDDPVGAIAVHGYCGTWGLISVGLFSIGIGNGILADASYAAAVPGLF
YGGGVSLLLIQLVAVLITIVWGFGLSFIIFKILDAVIGLRVSEEEEIMGLDICEHGIRAYPEYLMREE

Specific function: Involved In The Uptake Of Ammonia (Probable). [C]

COG id: COG0004

COG function: function code P; Ammonia permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ammonium transporter (TC 2.A.49) family [H]

Homologues:

Organism=Escherichia coli, GI1786656, Length=453, Percent_Identity=33.3333333333333, Blast_Score=177, Evalue=2e-45,
Organism=Caenorhabditis elegans, GI17550180, Length=419, Percent_Identity=40.3341288782816, Blast_Score=259, Evalue=3e-69,
Organism=Caenorhabditis elegans, GI17550182, Length=440, Percent_Identity=38.8636363636364, Blast_Score=239, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI32564042, Length=430, Percent_Identity=31.6279069767442, Blast_Score=184, Evalue=6e-47,
Organism=Caenorhabditis elegans, GI133931100, Length=437, Percent_Identity=32.0366132723112, Blast_Score=184, Evalue=1e-46,
Organism=Saccharomyces cerevisiae, GI6324187, Length=429, Percent_Identity=26.3403263403263, Blast_Score=123, Evalue=7e-29,
Organism=Saccharomyces cerevisiae, GI6325396, Length=443, Percent_Identity=27.313769751693, Blast_Score=115, Evalue=1e-26,
Organism=Saccharomyces cerevisiae, GI6321559, Length=441, Percent_Identity=26.0770975056689, Blast_Score=111, Evalue=3e-25,
Organism=Drosophila melanogaster, GI161078314, Length=423, Percent_Identity=37.3522458628842, Blast_Score=216, Evalue=3e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001905
- InterPro:   IPR018047 [H]

Pfam domain/function: PF00909 Ammonium_transp [H]

EC number: NA

Molecular weight: Translated: 49371; Mature: 49239

Theoretical pI: Translated: 5.31; Mature: 5.31

Prosite motif: PS01219 AMMONIUM_TRANSP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKNVQKIEKLLIVICMIFIVLPSPALAVTAEENAEAIEGLQTTLTFLWLLIASGLVFFM
CCCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
HAGFSLVETGLTRSKNTANILMKNFMTVVLGILVYWAVGWGVMYGADAAGLIGTDQFFLA
HCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEE
GADNATWNSWFFQMVFAATGATIVSGAMAERTNFKAYLVYCVLMVAVIYPIYGHWVWSGA
CCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCEEECCC
DTALLSGADSPIVKAIGVAHHDFAGSGVVHSIGGYSALAGVLLVGARIGKFRDGKAIPIP
CHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHCCCCCCCEECCC
GHNLTIAFLGTLILALGWLGFNGGSTLNANDPYMNLVVVNTFLAGAVGAVTTMAITWMKT
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCC
GKPDPSLTANGLLGGLVAVTAPCGSISNWGAIAIGIIAGIIIYAGVMFNENKLKLDDPVG
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCEEECCCCC
AIAVHGYCGTWGLISVGLFSIGIGNGILADASYAAAVPGLFYGGGVSLLLIQLVAVLITI
EEEEECCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHCCCHHCCCCHHHHHHHHHHHHHHH
VWGFGLSFIIFKILDAVIGLRVSEEEEIMGLDICEHGIRAYPEYLMREE
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SKNVQKIEKLLIVICMIFIVLPSPALAVTAEENAEAIEGLQTTLTFLWLLIASGLVFFM
CCHHHHHHHHHHHHHHHHHHCCCCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHH
HAGFSLVETGLTRSKNTANILMKNFMTVVLGILVYWAVGWGVMYGADAAGLIGTDQFFLA
HCCHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEE
GADNATWNSWFFQMVFAATGATIVSGAMAERTNFKAYLVYCVLMVAVIYPIYGHWVWSGA
CCCCCCHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCEEECCC
DTALLSGADSPIVKAIGVAHHDFAGSGVVHSIGGYSALAGVLLVGARIGKFRDGKAIPIP
CHHHHCCCCCHHHHHHHHHHCCCCCCCCHHHCCCHHHHHHHHHHHHHHCCCCCCCEECCC
GHNLTIAFLGTLILALGWLGFNGGSTLNANDPYMNLVVVNTFLAGAVGAVTTMAITWMKT
CCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCC
GKPDPSLTANGLLGGLVAVTAPCGSISNWGAIAIGIIAGIIIYAGVMFNENKLKLDDPVG
CCCCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCEEECCCCC
AIAVHGYCGTWGLISVGLFSIGIGNGILADASYAAAVPGLFYGGGVSLLLIQLVAVLITI
EEEEECCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHCCCHHCCCCHHHHHHHHHHHHHHH
VWGFGLSFIIFKILDAVIGLRVSEEEEIMGLDICEHGIRAYPEYLMREE
HHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NH3 [Periplasm] [C]

Specific reaction: NH3 [Periplasm] = NH3 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8590279; 8905231 [H]