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The map label for this gene is lipB [H]

Identifier: 21226955

GI number: 21226955

Start: 1007115

End: 1007855

Strand: Reverse

Name: lipB [H]

Synonym: MM_0853

Alternate gene names: 21226955

Gene position: 1007855-1007115 (Counterclockwise)

Preceding gene: 21226956

Following gene: 21226954

Centisome position: 24.6

GC content: 47.5

Gene sequence:

>741_bases
ATGAGACTGACTCTGCTCGGAACCGGGGACGCGGTAGGTACGCCCAAAATCGGGTGTAACTGCCCCGCATGCACTGATGC
TCGTGAAGGTGGAAAGAGCCAGCGCCTTCGTTTTTCTATCCTTGTGGAGTCCGGGCAGGGCAAAATTCTTATTGATACAA
GCCCCGACCTCAGGCAGCAGTTTCTCAGGCAAAAGCTTTCGGGTATAGATGCCGTAATCTGGACACACGGGCACTATGAC
CACTATGCAGGTTTCGGAGAGTTTTACCGGGTTCAAAATAAAGTCGATGTATATGGTGTTTGCGAAACTCTGGATTACAT
AGACCAGTATGTTTCTTTCCTGAAACCCAGGTATCACTACGTAAAACTCTATGAACCTTTTGAATTAATCGGGCTTGAGT
TTACCCTCTTCAAGGTTCATCACCCTCCTGTAGAAGTCCCTACCGGAGTTGTTATTCGGGATGGGGATACAAAGGTTGTT
GTTACAGGAGATACGAGCCCCGATATTCCTGAAAATAGCCTGGAGTTAATGAAAGACCCTGACCTCCTTATTGCAGATGC
CATCGTTCCTCCGCACATTCATATCAAAAAACACATGAACTCGGAAGAGGCTATGGCACTCGCACAGAAGCTAAATGCAA
AAGAAGTGGTCCTGACTCATCTCAGCCACCTCTTTCGCCCGCACCATATTGAATCAATGTTTTTACCTCTGGGGTACGAC
GGGCAGGTCTTTGAGTTTTAA

Upstream 100 bases:

>100_bases
AACGCAAGGTCCTTACCCAGAAGGACCTGCTTGACGCGGTTAATTCCTTTGACGAACGGGCAGACTTAAAGTCCGATGAG
TACGCAGGGAAGAAATCCTC

Downstream 100 bases:

>100_bases
CCTTTTTTAAATATGAGTTCTACACTTCATACAGGAGCTTTGAACTTCCCATATCCAGTTTTACTGTTCCAGGCATTTTT
TGCCCGGTATTGAAAGAGCG

Product: hypothetical protein

Products: NA

Alternate protein names: Lipoate-protein ligase B; Lipoyl/octanoyl transferase; Octanoyl-[acyl-carrier-protein]-protein N-octanoyltransferase [H]

Number of amino acids: Translated: 246; Mature: 246

Protein sequence:

>246_residues
MRLTLLGTGDAVGTPKIGCNCPACTDAREGGKSQRLRFSILVESGQGKILIDTSPDLRQQFLRQKLSGIDAVIWTHGHYD
HYAGFGEFYRVQNKVDVYGVCETLDYIDQYVSFLKPRYHYVKLYEPFELIGLEFTLFKVHHPPVEVPTGVVIRDGDTKVV
VTGDTSPDIPENSLELMKDPDLLIADAIVPPHIHIKKHMNSEEAMALAQKLNAKEVVLTHLSHLFRPHHIESMFLPLGYD
GQVFEF

Sequences:

>Translated_246_residues
MRLTLLGTGDAVGTPKIGCNCPACTDAREGGKSQRLRFSILVESGQGKILIDTSPDLRQQFLRQKLSGIDAVIWTHGHYD
HYAGFGEFYRVQNKVDVYGVCETLDYIDQYVSFLKPRYHYVKLYEPFELIGLEFTLFKVHHPPVEVPTGVVIRDGDTKVV
VTGDTSPDIPENSLELMKDPDLLIADAIVPPHIHIKKHMNSEEAMALAQKLNAKEVVLTHLSHLFRPHHIESMFLPLGYD
GQVFEF
>Mature_246_residues
MRLTLLGTGDAVGTPKIGCNCPACTDAREGGKSQRLRFSILVESGQGKILIDTSPDLRQQFLRQKLSGIDAVIWTHGHYD
HYAGFGEFYRVQNKVDVYGVCETLDYIDQYVSFLKPRYHYVKLYEPFELIGLEFTLFKVHHPPVEVPTGVVIRDGDTKVV
VTGDTSPDIPENSLELMKDPDLLIADAIVPPHIHIKKHMNSEEAMALAQKLNAKEVVLTHLSHLFRPHHIESMFLPLGYD
GQVFEF

Specific function: Catalyzes the transfer of endogenously produced octanoic acid from octanoyl-acyl-carrier-protein onto the lipoyl domains of lipoate-dependent enzymes. Lipoyl-ACP can also act as a substrate although octanoyl-ACP is likely to be the physiological substrate

COG id: COG1235

COG function: function code R; Metal-dependent hydrolases of the beta-lactamase superfamily I

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the lipB family [H]

Homologues:

Organism=Escherichia coli, GI1790530, Length=239, Percent_Identity=27.6150627615063, Blast_Score=77, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR004143
- InterPro:   IPR000544
- InterPro:   IPR020605 [H]

Pfam domain/function: PF03099 BPL_LipA_LipB; PF00753 Lactamase_B [H]

EC number: =2.3.1.181 [H]

Molecular weight: Translated: 27743; Mature: 27743

Theoretical pI: Translated: 6.31; Mature: 6.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRLTLLGTGDAVGTPKIGCNCPACTDAREGGKSQRLRFSILVESGQGKILIDTSPDLRQQ
CEEEEEECCCCCCCCCCCCCCCCCCCHHCCCCCCEEEEEEEEECCCCEEEEECCHHHHHH
FLRQKLSGIDAVIWTHGHYDHYAGFGEFYRVQNKVDVYGVCETLDYIDQYVSFLKPRYHY
HHHHHHCCCEEEEEECCCCHHHCCCHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCEEE
VKLYEPFELIGLEFTLFKVHHPPVEVPTGVVIRDGDTKVVVTGDTSPDIPENSLELMKDP
EEEECCHHHHCEEEEEEEEECCCCCCCCCEEEECCCEEEEEECCCCCCCCCCHHHHHCCC
DLLIADAIVPPHIHIKKHMNSEEAMALAQKLNAKEVVLTHLSHLFRPHHIESMFLPLGYD
CEEEECCCCCCCEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEECCCC
GQVFEF
CCEECC
>Mature Secondary Structure
MRLTLLGTGDAVGTPKIGCNCPACTDAREGGKSQRLRFSILVESGQGKILIDTSPDLRQQ
CEEEEEECCCCCCCCCCCCCCCCCCCHHCCCCCCEEEEEEEEECCCCEEEEECCHHHHHH
FLRQKLSGIDAVIWTHGHYDHYAGFGEFYRVQNKVDVYGVCETLDYIDQYVSFLKPRYHY
HHHHHHCCCEEEEEECCCCHHHCCCHHHHHCCCCCEEEHHHHHHHHHHHHHHHHCCCEEE
VKLYEPFELIGLEFTLFKVHHPPVEVPTGVVIRDGDTKVVVTGDTSPDIPENSLELMKDP
EEEECCHHHHCEEEEEEEEECCCCCCCCCEEEECCCEEEEEECCCCCCCCCCHHHHHCCC
DLLIADAIVPPHIHIKKHMNSEEAMALAQKLNAKEVVLTHLSHLFRPHHIESMFLPLGYD
CEEEECCCCCCCEEEECCCCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCEEEEECCCC
GQVFEF
CCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12949112 [H]