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The map label for this gene is proB [H]

Identifier: 21226920

GI number: 21226920

Start: 968646

End: 969803

Strand: Reverse

Name: proB [H]

Synonym: MM_0818

Alternate gene names: 21226920

Gene position: 969803-968646 (Counterclockwise)

Preceding gene: 21226921

Following gene: 21226919

Centisome position: 23.67

GC content: 45.68

Gene sequence:

>1158_bases
TTGACAGAAAGAGAACAATTTTTCCGTGATGTTAATAAAATCGTCATTAAGATCGGAACTTCTTCAATTACCAGAAAGGG
CTGTGACCATACCAGGGAAAACTGCAACATTGACCCGGCATTTATGGAAAGCATAGCCTTCCAGGTCTCAGAGCTCCGAA
AGCAGGGGAAAGAAGTAATTATTGTGAGTTCGGGAGCAATAGGTGTCGGGCTGAATGAGCTGGGCATAGCCCCCAAACCC
CGTGAGATCCCTATCAGACAGGCAGCTGCAGCGGTCGGGCAGAGTATGCTGATGCAGGACTGGAGCAGAGCTTTTTCCAG
ATACGGAATGAAGGTTGCCCAGATCCTTCTTACCTATGAATTCTATTCTGATAGAGTAACTTACCTCAACCTGAGAAACA
GCATCTCAACCCTGCTGGAGTATGGTGTTGTCCCGATAATAAACGAAAACGACTGTACCTGTACAAACGAGATTGAAGCA
ATCTTCGGGGACAATGACAAGCTCTCTGCAATGGTTGCGAGCAAAATCGATGCTGATCTTCTGATTATCCTTTCGGATAT
TGATGGGCTTTTTGACAGGAACCCGAAAACTCACATTGACGCAAAGCTTCTGACCATTGTGAAAAAGATCACGCCTGAGA
TTGAAAGTTATGGGGGCGACCCTACAAGCTTCAAAGGCGTTGGCGGGATGCGGACCAAAATAAAAGCTGCAAAAATCTGC
AGTATGGCAGGCTGCTATGTAGTTATTGCAAACAGCGATGTAGAGGACGTCATCCTGAAAATAGCCTCAGGGGAAGAAAT
AGGGACCCTTTTCCTTGCTGAACGGCATATCCAGAAAAACCGCGCTCGCTGGATTATCCTTGCCAGGGCTTCAGGAACGG
TCCGTGTAGATGCCGGAGCAAAAGCTGCTGTCCTCGGGAAAAATAGCCTTCTCCCGGCAGGCGTTGTGGATGTGGAAGGG
ACATTTGATAGGGGAGATGTCGTGAAACTCGAATGTGACGGCAAAATATTTGCAAAAGGGATTACCGACTATACCTCTGA
AGAGTTAATTAAAATAAAAGGAGTTCATACAGACCAGATTGAAAACGTTCTGGGCTACAGTAATTACAACAACGTGATAA
AAAAGGAAAATATCGGCATACTGGAAGAGTTAAATTGA

Upstream 100 bases:

>100_bases
AATCGAAATACCAGCGCAGGTTAAGTGCAAAAACCGGAAACCCGGCTATTATAGAAATCAAGCGCAGCCGCTTTCCAAAT
AACTTTAAGAGGCACTTCTA

Downstream 100 bases:

>100_bases
GCATCGGGTTTGTAAGAGTTTCAGGACCTTCTGGTTCTTTACAGGTAATTTACGGTAATTTAAGGTTAATTTAAGGTTAA
TTTAAGGTTAATTTAAGTTT

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK [H]

Number of amino acids: Translated: 385; Mature: 384

Protein sequence:

>385_residues
MTEREQFFRDVNKIVIKIGTSSITRKGCDHTRENCNIDPAFMESIAFQVSELRKQGKEVIIVSSGAIGVGLNELGIAPKP
REIPIRQAAAAVGQSMLMQDWSRAFSRYGMKVAQILLTYEFYSDRVTYLNLRNSISTLLEYGVVPIINENDCTCTNEIEA
IFGDNDKLSAMVASKIDADLLIILSDIDGLFDRNPKTHIDAKLLTIVKKITPEIESYGGDPTSFKGVGGMRTKIKAAKIC
SMAGCYVVIANSDVEDVILKIASGEEIGTLFLAERHIQKNRARWIILARASGTVRVDAGAKAAVLGKNSLLPAGVVDVEG
TFDRGDVVKLECDGKIFAKGITDYTSEELIKIKGVHTDQIENVLGYSNYNNVIKKENIGILEELN

Sequences:

>Translated_385_residues
MTEREQFFRDVNKIVIKIGTSSITRKGCDHTRENCNIDPAFMESIAFQVSELRKQGKEVIIVSSGAIGVGLNELGIAPKP
REIPIRQAAAAVGQSMLMQDWSRAFSRYGMKVAQILLTYEFYSDRVTYLNLRNSISTLLEYGVVPIINENDCTCTNEIEA
IFGDNDKLSAMVASKIDADLLIILSDIDGLFDRNPKTHIDAKLLTIVKKITPEIESYGGDPTSFKGVGGMRTKIKAAKIC
SMAGCYVVIANSDVEDVILKIASGEEIGTLFLAERHIQKNRARWIILARASGTVRVDAGAKAAVLGKNSLLPAGVVDVEG
TFDRGDVVKLECDGKIFAKGITDYTSEELIKIKGVHTDQIENVLGYSNYNNVIKKENIGILEELN
>Mature_384_residues
TEREQFFRDVNKIVIKIGTSSITRKGCDHTRENCNIDPAFMESIAFQVSELRKQGKEVIIVSSGAIGVGLNELGIAPKPR
EIPIRQAAAAVGQSMLMQDWSRAFSRYGMKVAQILLTYEFYSDRVTYLNLRNSISTLLEYGVVPIINENDCTCTNEIEAI
FGDNDKLSAMVASKIDADLLIILSDIDGLFDRNPKTHIDAKLLTIVKKITPEIESYGGDPTSFKGVGGMRTKIKAAKICS
MAGCYVVIANSDVEDVILKIASGEEIGTLFLAERHIQKNRARWIILARASGTVRVDAGAKAAVLGKNSLLPAGVVDVEGT
FDRGDVVKLECDGKIFAKGITDYTSEELIKIKGVHTDQIENVLGYSNYNNVIKKENIGILEELN

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline [H]

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain [H]

Homologues:

Organism=Homo sapiens, GI62912457, Length=294, Percent_Identity=32.9931972789116, Blast_Score=133, Evalue=3e-31,
Organism=Homo sapiens, GI21361368, Length=296, Percent_Identity=32.7702702702703, Blast_Score=132, Evalue=8e-31,
Organism=Escherichia coli, GI1786437, Length=359, Percent_Identity=38.7186629526462, Blast_Score=239, Evalue=2e-64,
Organism=Caenorhabditis elegans, GI17569967, Length=277, Percent_Identity=31.7689530685921, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI17569969, Length=277, Percent_Identity=31.7689530685921, Blast_Score=130, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6320506, Length=424, Percent_Identity=36.3207547169811, Blast_Score=226, Evalue=6e-60,
Organism=Saccharomyces cerevisiae, GI6321822, Length=422, Percent_Identity=35.3080568720379, Blast_Score=208, Evalue=1e-54,
Organism=Drosophila melanogaster, GI21357643, Length=307, Percent_Identity=34.8534201954397, Blast_Score=136, Evalue=2e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947 [H]

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA [H]

EC number: =2.7.2.11 [H]

Molecular weight: Translated: 42258; Mature: 42127

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: PS50890 PUA ; PS00902 GLUTAMATE_5_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEREQFFRDVNKIVIKIGTSSITRKGCDHTRENCNIDPAFMESIAFQVSELRKQGKEVI
CCCHHHHHHHHHHEEEEECCCHHHHCCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCCEEE
IVSSGAIGVGLNELGIAPKPREIPIRQAAAAVGQSMLMQDWSRAFSRYGMKVAQILLTYE
EEECCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
FYSDRVTYLNLRNSISTLLEYGVVPIINENDCTCTNEIEAIFGDNDKLSAMVASKIDADL
HHCCCEEEEEHHHHHHHHHHCCCEEEECCCCCCCCCCHHEEECCCCHHHHHHHHHCCCCE
LIILSDIDGLFDRNPKTHIDAKLLTIVKKITPEIESYGGDPTSFKGVGGMRTKIKAAKIC
EEEEEHHHHHHCCCCCCHHHHHHHHHHHHHCHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
SMAGCYVVIANSDVEDVILKIASGEEIGTLFLAERHIQKNRARWIILARASGTVRVDAGA
HCCCEEEEEECCCHHHHHEECCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCEEEECCCC
KAAVLGKNSLLPAGVVDVEGTFDRGDVVKLECDGKIFAKGITDYTSEELIKIKGVHTDQI
EEEEECCCCCCCCCEEECCCCCCCCCEEEEEECCEEEECCCCCCCCCCEEEEECCCHHHH
ENVLGYSNYNNVIKKENIGILEELN
HHHHCCCCCCCCHHHCCCCCCCCCC
>Mature Secondary Structure 
TEREQFFRDVNKIVIKIGTSSITRKGCDHTRENCNIDPAFMESIAFQVSELRKQGKEVI
CCHHHHHHHHHHEEEEECCCHHHHCCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCCEEE
IVSSGAIGVGLNELGIAPKPREIPIRQAAAAVGQSMLMQDWSRAFSRYGMKVAQILLTYE
EEECCCCCCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHH
FYSDRVTYLNLRNSISTLLEYGVVPIINENDCTCTNEIEAIFGDNDKLSAMVASKIDADL
HHCCCEEEEEHHHHHHHHHHCCCEEEECCCCCCCCCCHHEEECCCCHHHHHHHHHCCCCE
LIILSDIDGLFDRNPKTHIDAKLLTIVKKITPEIESYGGDPTSFKGVGGMRTKIKAAKIC
EEEEEHHHHHHCCCCCCHHHHHHHHHHHHHCHHHHHCCCCCCCCCCCCCCHHHHHHHHHH
SMAGCYVVIANSDVEDVILKIASGEEIGTLFLAERHIQKNRARWIILARASGTVRVDAGA
HCCCEEEEEECCCHHHHHEECCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCEEEECCCC
KAAVLGKNSLLPAGVVDVEGTFDRGDVVKLECDGKIFAKGITDYTSEELIKIKGVHTDQI
EEEEECCCCCCCCCEEECCCCCCCCCEEEEEECCEEEECCCCCCCCCCEEEEECCCHHHH
ENVLGYSNYNNVIKKENIGILEELN
HHHHCCCCCCCCHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11844777; 11932238 [H]