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The map label for this gene is nifK2 [H]

Identifier: 21226827

GI number: 21226827

Start: 872472

End: 874052

Strand: Direct

Name: nifK2 [H]

Synonym: MM_0725

Alternate gene names: 21226827

Gene position: 872472-874052 (Clockwise)

Preceding gene: 21226826

Following gene: 21226828

Centisome position: 21.3

GC content: 49.84

Gene sequence:

>1581_bases
ATGACTAAAAGAAACTATGCAACCGTGAACCCCTGTGTTATGTGCCAGCCCATGGGAAGTGCCCTTGCCTTTAAAGGAAT
TGAAAACACCATGGTCCTCTACCACGGCTCCCAGGGATGCAGCACCTATATGCGCCTGCACCTTGCCCACCATTTCAGGG
AGCCTGTGGATATTGCCTCAAGCTCCCTGAGCGAAAAAGGGGCTGTCTACGGGGGCAGGGAAAACCTGAAAAAGGGACTC
AGGAACGTCATCAACAGGTACAGACCGAAGGTGATAGGTGTTGCGACCACCTGCCTTGCAGAAACGATCGGCGATGATGT
TCCTGCGATTATCAGGGAATTCAAAGAGGAAGAAGGGATCGGAGACAACCCTGAAAAAGATATAATAATCATTCCGGTTT
CCACTCCCAGCTATGGGGAAAGCCATGTCAGCGGATATATAAAATCCCTGGATGCCATTGTAAGGAAATTTACGGAAAAA
CCTGAAAGTCCGGAAAAACCGAAAATCCCGGAAGACACCGAAATCATGGATTACATGGAAAACATGGAAATTATGGGGTA
TACGGACATCCTGGAAAAAGAAAAGGCAAGGGAAAGCATGGGGAATGCAAAAAGTCCTGGCATGAAGAAAGAAGCACCCT
ATCCGATTAAAACTTCAAACGGCAAGCTCAACATTATCTCTGTCGAGAGCGTCTCCCCGGCTGATGTGAGGGAAATGAAA
GAGATCCTTGCAGAGACCGCAGGGGGCTACATCTTTCTCCCGGACATTTCCGATACCTTTGATGCACCACTTCGGGACCA
TCTTCCAAAAATCGCTCCCGGAGGCACTCCGCTTTCGGAGATTGCCGATATGCCTGACAGCAGAGCAAGCCTGGGCCTGG
GTGTTGTCAGCACGAACCTGGCTGTGAAATACCTCGAAACTTCCCACGGGGTTCCCGGACACAACGTCCCCATCCCCATA
GGGCTCTCAAACACCGACCTTTTCTTTATGGAACTGGTCCGTATCCTTGGCTGCCCTATCCCTGAGAAATACCAGAAAGA
AAGGGGAAGGCTACTCGATGCCATGGTAGATGTGCATAAGTACCTCTATGGAGTAAAGGCAGCGGTCTACGGGGACCCGG
ACACTGTATTTAGCCTGACGACCTTCATGCTGGAACTCGGGATGAATCCCGTCCTGGTAACAACCGGAAGCAAAAGCCGG
AACTTCGAAAAGAGAATAAAACAAATCTTTGAAGAGATCAGGCCGGAACTGGAACCTGTAGTTCTGGACGGGATTGACTT
TGACAGCCTTAAAGATGCAGTAAAGGAATGCAGTCCCGAAATCCTGATAGGAAACTCCAACGGGAGATATATTGCCAGAG
CACTCGACATCCCGCTTGTAAGGGTTGGACTGCCAATCCATGACAGGGTAGGCGCACAGCGCATCCTTACTGTCGGGTAC
AGGGGAGCCCTGGAACTTCTGGACAGGATCACAAACATCATCCTTGAAGCTACGGATACTTTCACCGCGCCTGTACAGCC
GGAATCTGAAATATATGCCGGTAATGGAGCAGAAAACGAATATGCAGAGGAATTGCAGTGA

Upstream 100 bases:

>100_bases
ACCATACTCAAAGAAGTGTAAGGGTCTAGCCCCGAAACCGGAGTTTTTACACTCCGGACAGGATGCTGTAGTTGAGAGTG
AGGTATGAGGAGGCAATGAG

Downstream 100 bases:

>100_bases
GCGGCAGAAGGGAAAAAAGCGACAGAAAGGCAAAAAAACTGATACTTCCGGCTTTTGCAGCAATTCTCTGTCTTGCCGTA
CTTACCTTTATTGCAGTCTC

Product: nitrogenase iron-molybdenum cofactor biosynthesis protein NifN

Products: NA

Alternate protein names: Dinitrogenase; Nitrogenase component I [H]

Number of amino acids: Translated: 526; Mature: 525

Protein sequence:

>526_residues
MTKRNYATVNPCVMCQPMGSALAFKGIENTMVLYHGSQGCSTYMRLHLAHHFREPVDIASSSLSEKGAVYGGRENLKKGL
RNVINRYRPKVIGVATTCLAETIGDDVPAIIREFKEEEGIGDNPEKDIIIIPVSTPSYGESHVSGYIKSLDAIVRKFTEK
PESPEKPKIPEDTEIMDYMENMEIMGYTDILEKEKARESMGNAKSPGMKKEAPYPIKTSNGKLNIISVESVSPADVREMK
EILAETAGGYIFLPDISDTFDAPLRDHLPKIAPGGTPLSEIADMPDSRASLGLGVVSTNLAVKYLETSHGVPGHNVPIPI
GLSNTDLFFMELVRILGCPIPEKYQKERGRLLDAMVDVHKYLYGVKAAVYGDPDTVFSLTTFMLELGMNPVLVTTGSKSR
NFEKRIKQIFEEIRPELEPVVLDGIDFDSLKDAVKECSPEILIGNSNGRYIARALDIPLVRVGLPIHDRVGAQRILTVGY
RGALELLDRITNIILEATDTFTAPVQPESEIYAGNGAENEYAEELQ

Sequences:

>Translated_526_residues
MTKRNYATVNPCVMCQPMGSALAFKGIENTMVLYHGSQGCSTYMRLHLAHHFREPVDIASSSLSEKGAVYGGRENLKKGL
RNVINRYRPKVIGVATTCLAETIGDDVPAIIREFKEEEGIGDNPEKDIIIIPVSTPSYGESHVSGYIKSLDAIVRKFTEK
PESPEKPKIPEDTEIMDYMENMEIMGYTDILEKEKARESMGNAKSPGMKKEAPYPIKTSNGKLNIISVESVSPADVREMK
EILAETAGGYIFLPDISDTFDAPLRDHLPKIAPGGTPLSEIADMPDSRASLGLGVVSTNLAVKYLETSHGVPGHNVPIPI
GLSNTDLFFMELVRILGCPIPEKYQKERGRLLDAMVDVHKYLYGVKAAVYGDPDTVFSLTTFMLELGMNPVLVTTGSKSR
NFEKRIKQIFEEIRPELEPVVLDGIDFDSLKDAVKECSPEILIGNSNGRYIARALDIPLVRVGLPIHDRVGAQRILTVGY
RGALELLDRITNIILEATDTFTAPVQPESEIYAGNGAENEYAEELQ
>Mature_525_residues
TKRNYATVNPCVMCQPMGSALAFKGIENTMVLYHGSQGCSTYMRLHLAHHFREPVDIASSSLSEKGAVYGGRENLKKGLR
NVINRYRPKVIGVATTCLAETIGDDVPAIIREFKEEEGIGDNPEKDIIIIPVSTPSYGESHVSGYIKSLDAIVRKFTEKP
ESPEKPKIPEDTEIMDYMENMEIMGYTDILEKEKARESMGNAKSPGMKKEAPYPIKTSNGKLNIISVESVSPADVREMKE
ILAETAGGYIFLPDISDTFDAPLRDHLPKIAPGGTPLSEIADMPDSRASLGLGVVSTNLAVKYLETSHGVPGHNVPIPIG
LSNTDLFFMELVRILGCPIPEKYQKERGRLLDAMVDVHKYLYGVKAAVYGDPDTVFSLTTFMLELGMNPVLVTTGSKSRN
FEKRIKQIFEEIRPELEPVVLDGIDFDSLKDAVKECSPEILIGNSNGRYIARALDIPLVRVGLPIHDRVGAQRILTVGYR
GALELLDRITNIILEATDTFTAPVQPESEIYAGNGAENEYAEELQ

Specific function: This molybdenum-iron protein is part of the nitrogenase complex that catalyzes the key enzymatic reactions in nitrogen fixation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005976 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: =1.18.6.1 [H]

Molecular weight: Translated: 57965; Mature: 57834

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTKRNYATVNPCVMCQPMGSALAFKGIENTMVLYHGSQGCSTYMRLHLAHHFREPVDIAS
CCCCCCCCCCCEEEECCCCCHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHCCHHHHHH
SSLSEKGAVYGGRENLKKGLRNVINRYRPKVIGVATTCLAETIGDDVPAIIREFKEEEGI
HHHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHCCCHHHHHHHHHHHCCC
GDNPEKDIIIIPVSTPSYGESHVSGYIKSLDAIVRKFTEKPESPEKPKIPEDTEIMDYME
CCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
NMEIMGYTDILEKEKARESMGNAKSPGMKKEAPYPIKTSNGKLNIISVESVSPADVREMK
CCHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCEEEEEEECCCCCHHHHHHH
EILAETAGGYIFLPDISDTFDAPLRDHLPKIAPGGTPLSEIADMPDSRASLGLGVVSTNL
HHHHHHCCCEEEECCCCCCCCCCHHHCCCCCCCCCCCHHHHHCCCCCHHHCCCEEEEHHH
AVKYLETSHGVPGHNVPIPIGLSNTDLFFMELVRILGCPIPEKYQKERGRLLDAMVDVHK
EEEEEHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
YLYGVKAAVYGDPDTVFSLTTFMLELGMNPVLVTTGSKSRNFEKRIKQIFEEIRPELEPV
HHHHHHHEEECCCHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCE
VLDGIDFDSLKDAVKECSPEILIGNSNGRYIARALDIPLVRVGLPIHDRVGAQRILTVGY
EECCCCHHHHHHHHHHCCCCEEEECCCCCEEEEEECCCEEEECCCHHHHHCHHHHHHHCH
RGALELLDRITNIILEATDTFTAPVQPESEIYAGNGAENEYAEELQ
HHHHHHHHHHHHHHHHCCCCEECCCCCCCCEEECCCCCHHHHHHCC
>Mature Secondary Structure 
TKRNYATVNPCVMCQPMGSALAFKGIENTMVLYHGSQGCSTYMRLHLAHHFREPVDIAS
CCCCCCCCCCEEEECCCCCHHHHCCCCCEEEEEECCCCHHHHHHHHHHHHHCCHHHHHH
SSLSEKGAVYGGRENLKKGLRNVINRYRPKVIGVATTCLAETIGDDVPAIIREFKEEEGI
HHHHHCCCCCCCHHHHHHHHHHHHHHHCCCEEHHHHHHHHHHHCCCHHHHHHHHHHHCCC
GDNPEKDIIIIPVSTPSYGESHVSGYIKSLDAIVRKFTEKPESPEKPKIPEDTEIMDYME
CCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHH
NMEIMGYTDILEKEKARESMGNAKSPGMKKEAPYPIKTSNGKLNIISVESVSPADVREMK
CCHHCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEECCCCEEEEEEECCCCCHHHHHHH
EILAETAGGYIFLPDISDTFDAPLRDHLPKIAPGGTPLSEIADMPDSRASLGLGVVSTNL
HHHHHHCCCEEEECCCCCCCCCCHHHCCCCCCCCCCCHHHHHCCCCCHHHCCCEEEEHHH
AVKYLETSHGVPGHNVPIPIGLSNTDLFFMELVRILGCPIPEKYQKERGRLLDAMVDVHK
EEEEEHHCCCCCCCCCCEEECCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHH
YLYGVKAAVYGDPDTVFSLTTFMLELGMNPVLVTTGSKSRNFEKRIKQIFEEIRPELEPV
HHHHHHHEEECCCHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCCE
VLDGIDFDSLKDAVKECSPEILIGNSNGRYIARALDIPLVRVGLPIHDRVGAQRILTVGY
EECCCCHHHHHHHHHHCCCCEEEECCCCCEEEEEECCCEEEECCCHHHHHCHHHHHHHCH
RGALELLDRITNIILEATDTFTAPVQPESEIYAGNGAENEYAEELQ
HHHHHHHHHHHHHHHHCCCCEECCCCCCCCEEECCCCCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8550408; 7961410 [H]