Click here to switch to the map view.

The map label for this gene is nifE [H]

Identifier: 21226826

GI number: 21226826

Start: 870843

End: 872459

Strand: Direct

Name: nifE [H]

Synonym: MM_0724

Alternate gene names: 21226826

Gene position: 870843-872459 (Clockwise)

Preceding gene: 21226825

Following gene: 21226827

Centisome position: 21.26

GC content: 47.62

Gene sequence:

>1617_bases
GTGAAAGAGATGAGTGAGAAAACCGGGATAGTGGATACTCTCGAAGAAAGGCAACCGTATATTACCAGAAAACAGGAAAA
GGGGCAGGAAATTCCCCTTGCCTGCGACAATAACTCCCTTGCAGGAGCCATTAGCCAGCGGGCATGTGTATATTCGGGAG
CAAGGGTTGTCTTAAACCCTGTAACCGATGCAGTCCATCTGGTCCACGGCCCCATAGGCTGTGCAGGGTATACCTGGGAC
ATAAGGGGTGCAAAGTCCAGCGGGGTCGAAACGAACCGCAGCAGTTTCAGCACGGACATGAAAGAGATCGATGTTGTCTT
CGGAGGAGAGAAAAAACTCTCAAATGCAATTGATGAGCTGGCAGGTATCTACCGCCCTCCTGTCATTTTCGTGTATTCTA
CATGCATTGTGGGAATTATCGGAGATGACCTGGAAGCTGTATGCAAAACAGCAAGCAAGAAGCACAATATCCCGGTAATC
CCTGTAAAGTCCGAAGGGTTCAAAGGCAACAAGTCCGACGGATACAAAGCTGCATGCAATGCCTTAAAGCAGCTTATAAA
AAGACCTGAAAAGAAGACTGTAGAAAAAAAACCCGAAATAAGGTCTGAATCTAAAAAACCGGATATTAAACACTCAAAAA
TCGAGAAGATGGAAATCAAAAAACCGAAGATCAATATCCTCGGGGACTTCAACGTGGCTGGGGATGTCTGGCTCGTAAAA
CCCCTCTTTGAAAAGATGGGAATCGAAGTCATAGTCTCCATGACCGGAGATTCGACTGCCGAATCCATTTCAAGGGCAGC
GGAAGCAGACCTCAACCTCGTCCAGTGCAGCGGATCAATGACCTATCTTGCAAAATGGATGCAGCAGGAGTACGGGATTC
CATATCAGACTGTAAGCTTCTTCGGAATCGAAGATATTTCCATTGCTCTTCGGAAAACTGCGGAATACTTTGGCTCGGAA
GAAATGAAAAAGAAAGCAGAAAAGATTCTTGAAGCCGAAACAAACCGTATCATGCCTGAAATTTCCCGTGTAAGAGAGAG
TATCAAAGGGAAAAAGGCCGCAATCTATATGGGAGGCCCTGCCAAAGCTCTCACGCTTATCAAAGGTTTTGCGGAACTGG
GCATGGAAGTGGTCATTATAGGGACCCAGACCGGGAAAAAGGAAGACTACGAGCAGATCAGCTATTCGGTCCGGGACGGG
ACGGTCATTGTGGATGATGCAAACCCCCTGGAACTTGCAGACCTGCTCGTGAAACAGAAAGCCGACCTGATGGTTGCAGG
CGTGAAAGAGAGGTTTATCGCATACAAGCTGGGCGTTGCTTTCTGCGATTTCAACCACGACAGGGTGGTGGAGTTCGAAG
GCTTTGACGGCTTTGTAAACTTTGCAAAGGAAGTGGATGCTTCCATTAACAGCCCTGTCTGGAAAGCTGTCAGGCAGAGG
ACCCTTAACCCCGAACCCCTGGAGCCGAAAAAAACCATGGTAGAATCGGGACAGGAAAAAGCCACAATCAAAGAAGAAGT
TTCCGAAAAACCATACTCAAAGAAGTGTAAGGGTCTAGCCCCGAAACCGGAGTTTTTACACTCCGGACAGGATGCTGTAG
TTGAGAGTGAGGTATGA

Upstream 100 bases:

>100_bases
GAGATGACAGCAAACTGATAAATTCAAAATAGGAAAAAGTGGAAAACCGCCGCTGCAGTTCTGAAAGCAGGTAAAAATCT
GAAAACAGAAGCAGAGGGGA

Downstream 100 bases:

>100_bases
GGAGGCAATGAGATGACTAAAAGAAACTATGCAACCGTGAACCCCTGTGTTATGTGCCAGCCCATGGGAAGTGCCCTTGC
CTTTAAAGGAATTGAAAACA

Product: nitrogenase iron-molybdenum cofactor biosynthesis protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 538; Mature: 538

Protein sequence:

>538_residues
MKEMSEKTGIVDTLEERQPYITRKQEKGQEIPLACDNNSLAGAISQRACVYSGARVVLNPVTDAVHLVHGPIGCAGYTWD
IRGAKSSGVETNRSSFSTDMKEIDVVFGGEKKLSNAIDELAGIYRPPVIFVYSTCIVGIIGDDLEAVCKTASKKHNIPVI
PVKSEGFKGNKSDGYKAACNALKQLIKRPEKKTVEKKPEIRSESKKPDIKHSKIEKMEIKKPKINILGDFNVAGDVWLVK
PLFEKMGIEVIVSMTGDSTAESISRAAEADLNLVQCSGSMTYLAKWMQQEYGIPYQTVSFFGIEDISIALRKTAEYFGSE
EMKKKAEKILEAETNRIMPEISRVRESIKGKKAAIYMGGPAKALTLIKGFAELGMEVVIIGTQTGKKEDYEQISYSVRDG
TVIVDDANPLELADLLVKQKADLMVAGVKERFIAYKLGVAFCDFNHDRVVEFEGFDGFVNFAKEVDASINSPVWKAVRQR
TLNPEPLEPKKTMVESGQEKATIKEEVSEKPYSKKCKGLAPKPEFLHSGQDAVVESEV

Sequences:

>Translated_538_residues
MKEMSEKTGIVDTLEERQPYITRKQEKGQEIPLACDNNSLAGAISQRACVYSGARVVLNPVTDAVHLVHGPIGCAGYTWD
IRGAKSSGVETNRSSFSTDMKEIDVVFGGEKKLSNAIDELAGIYRPPVIFVYSTCIVGIIGDDLEAVCKTASKKHNIPVI
PVKSEGFKGNKSDGYKAACNALKQLIKRPEKKTVEKKPEIRSESKKPDIKHSKIEKMEIKKPKINILGDFNVAGDVWLVK
PLFEKMGIEVIVSMTGDSTAESISRAAEADLNLVQCSGSMTYLAKWMQQEYGIPYQTVSFFGIEDISIALRKTAEYFGSE
EMKKKAEKILEAETNRIMPEISRVRESIKGKKAAIYMGGPAKALTLIKGFAELGMEVVIIGTQTGKKEDYEQISYSVRDG
TVIVDDANPLELADLLVKQKADLMVAGVKERFIAYKLGVAFCDFNHDRVVEFEGFDGFVNFAKEVDASINSPVWKAVRQR
TLNPEPLEPKKTMVESGQEKATIKEEVSEKPYSKKCKGLAPKPEFLHSGQDAVVESEV
>Mature_538_residues
MKEMSEKTGIVDTLEERQPYITRKQEKGQEIPLACDNNSLAGAISQRACVYSGARVVLNPVTDAVHLVHGPIGCAGYTWD
IRGAKSSGVETNRSSFSTDMKEIDVVFGGEKKLSNAIDELAGIYRPPVIFVYSTCIVGIIGDDLEAVCKTASKKHNIPVI
PVKSEGFKGNKSDGYKAACNALKQLIKRPEKKTVEKKPEIRSESKKPDIKHSKIEKMEIKKPKINILGDFNVAGDVWLVK
PLFEKMGIEVIVSMTGDSTAESISRAAEADLNLVQCSGSMTYLAKWMQQEYGIPYQTVSFFGIEDISIALRKTAEYFGSE
EMKKKAEKILEAETNRIMPEISRVRESIKGKKAAIYMGGPAKALTLIKGFAELGMEVVIIGTQTGKKEDYEQISYSVRDG
TVIVDDANPLELADLLVKQKADLMVAGVKERFIAYKLGVAFCDFNHDRVVEFEGFDGFVNFAKEVDASINSPVWKAVRQR
TLNPEPLEPKKTMVESGQEKATIKEEVSEKPYSKKCKGLAPKPEFLHSGQDAVVESEV

Specific function: This protein may play a role in the biosynthesis of the prosthetic group of nitrogenase (FeMo cofactor) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nifD/nifK/nifE/nifN family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000510
- InterPro:   IPR000318
- InterPro:   IPR005973 [H]

Pfam domain/function: PF00148 Oxidored_nitro [H]

EC number: NA

Molecular weight: Translated: 59399; Mature: 59399

Theoretical pI: Translated: 7.38; Mature: 7.38

Prosite motif: PS00699 NITROGENASE_1_1 ; PS00090 NITROGENASE_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKEMSEKTGIVDTLEERQPYITRKQEKGQEIPLACDNNSLAGAISQRACVYSGARVVLNP
CCCCHHHCCCHHHHHHCCCCCCCHHHCCCCCCEEECCCCHHHHHHHHHHHHCCCEEEEEC
VTDAVHLVHGPIGCAGYTWDIRGAKSSGVETNRSSFSTDMKEIDVVFGGEKKLSNAIDEL
HHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCHHHHCCHHHEEEEECCHHHHHHHHHHH
AGIYRPPVIFVYSTCIVGIIGDDLEAVCKTASKKHNIPVIPVKSEGFKGNKSDGYKAACN
HHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCHHHHHH
ALKQLIKRPEKKTVEKKPEIRSESKKPDIKHSKIEKMEIKKPKINILGDFNVAGDVWLVK
HHHHHHHCCHHHHHHCCCCHHCCCCCCCCCHHHHHHHCCCCCCEEEEECCCCCCCCEEHH
PLFEKMGIEVIVSMTGDSTAESISRAAEADLNLVQCSGSMTYLAKWMQQEYGIPYQTVSF
HHHHHCCEEEEEEECCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHCCCCCCEEEE
FGIEDISIALRKTAEYFGSEEMKKKAEKILEAETNRIMPEISRVRESIKGKKAAIYMGGP
ECCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCC
AKALTLIKGFAELGMEVVIIGTQTGKKEDYEQISYSVRDGTVIVDDANPLELADLLVKQK
HHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHEEECCCEEEEECCCHHHHHHHHHHHH
ADLMVAGVKERFIAYKLGVAFCDFNHDRVVEFEGFDGFVNFAKEVDASINSPVWKAVRQR
CCCEEHHHHHHHHHHHHHHEEEECCCCEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHH
TLNPEPLEPKKTMVESGQEKATIKEEVSEKPYSKKCKGLAPKPEFLHSGQDAVVESEV
CCCCCCCCHHHHHHHCCCHHHHHHHHHHCCCCHHHHCCCCCCHHHHHCCCCCEECCCC
>Mature Secondary Structure
MKEMSEKTGIVDTLEERQPYITRKQEKGQEIPLACDNNSLAGAISQRACVYSGARVVLNP
CCCCHHHCCCHHHHHHCCCCCCCHHHCCCCCCEEECCCCHHHHHHHHHHHHCCCEEEEEC
VTDAVHLVHGPIGCAGYTWDIRGAKSSGVETNRSSFSTDMKEIDVVFGGEKKLSNAIDEL
HHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCHHHHCCHHHEEEEECCHHHHHHHHHHH
AGIYRPPVIFVYSTCIVGIIGDDLEAVCKTASKKHNIPVIPVKSEGFKGNKSDGYKAACN
HHHCCCCHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCEEEECCCCCCCCCCCCHHHHHH
ALKQLIKRPEKKTVEKKPEIRSESKKPDIKHSKIEKMEIKKPKINILGDFNVAGDVWLVK
HHHHHHHCCHHHHHHCCCCHHCCCCCCCCCHHHHHHHCCCCCCEEEEECCCCCCCCEEHH
PLFEKMGIEVIVSMTGDSTAESISRAAEADLNLVQCSGSMTYLAKWMQQEYGIPYQTVSF
HHHHHCCEEEEEEECCCCHHHHHHHHHHCCCEEEEECCCHHHHHHHHHHHCCCCCCEEEE
FGIEDISIALRKTAEYFGSEEMKKKAEKILEAETNRIMPEISRVRESIKGKKAAIYMGGP
ECCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCC
AKALTLIKGFAELGMEVVIIGTQTGKKEDYEQISYSVRDGTVIVDDANPLELADLLVKQK
HHHHHHHHHHHHCCCEEEEEECCCCCCCCHHHHHEEECCCEEEEECCCHHHHHHHHHHHH
ADLMVAGVKERFIAYKLGVAFCDFNHDRVVEFEGFDGFVNFAKEVDASINSPVWKAVRQR
CCCEEHHHHHHHHHHHHHHEEEECCCCEEEEECCCCHHHHHHHHHCCCCCCHHHHHHHHH
TLNPEPLEPKKTMVESGQEKATIKEEVSEKPYSKKCKGLAPKPEFLHSGQDAVVESEV
CCCCCCCCHHHHHHHCCCHHHHHHHHHHCCCCHHHHCCCCCCHHHHHCCCCCEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8550408 [H]