Click here to switch to the map view.

The map label for this gene is trmE

Identifier: 20809125

GI number: 20809125

Start: 2685852

End: 2687234

Strand: Reverse

Name: trmE

Synonym: TTE2796

Alternate gene names: 20809125

Gene position: 2687234-2685852 (Counterclockwise)

Preceding gene: 20809126

Following gene: 20809124

Centisome position: 99.92

GC content: 36.95

Gene sequence:

>1383_bases
ATGGAATTTGATACAATTGCAGCTATCTCTACTTTCCCTGGAGAAGCGGGAATTGGAATAGTGAGAATAAGTGGAGATGA
AGCTTTAGAAATAATTTCAAAAATTTTTAGACCTTTCAGAAAAAAAGATATAAAATCTGTTAAAAGCCACACTATCCACT
ATGGTCATATTGTAGACCCTGAAACGGGTGAAGTTTACGATGAAGTGCTGGTAACTGTCATGAGGAAGCCCAATACTTAC
ACTAGAGAAGATGTAGTGGAAATTAACTGCCATGGGGGTATAGTAGTAAGCTCAAAGATTTTAGAACTGGTTTTAAAACA
TGGAGCGCGCCTTGCTGAACCAGGGGAGTTTACTAAAAGAGCTTTTTTAAATGGGAGGATTGATTTGTCACAGGCAGAAG
CTGTGATTGATATTATAACATCTAAAACTATGCTTGCTAATAGATATGCTCAAAAGCAGCTGGCAGGGGTTTTGGGGCAG
AAGATGAAAGATTTGAAAAACAAGATAATGGAACTTTTGAGTCATCTTTTGGCTTTGATTGACTTTCCTGAAGAGGATGT
GGAAGAGCTTGAAAGAGAGGAAATTAAAAGAAGGGCAAAAGACATATTAAATGACATAGAATATTTGATTGCTTCTTCTG
AAAGCGGAAGGATAATAAGGGAAGGGCTCAAGACAGCTATAATAGGAAAGCCTAATGTGGGGAAGTCCTCTCTTTTAAAT
GCTCTTTTAAAGCAAAATAGAGCTATAGTCACTGACATCCCTGGGACCACAAGGGATGTAATAGAGGAGTACATGAATAT
AAAAGGAATTCCAATAAAGCTCATAGACACTGCAGGAATAAGGCATACAGATGAACTGGTGGAAAAAATAGGAGTGGAAA
AGAGCAAAGAAGTACTGGCAGAAGCTGACCTTATTTTATTTGTTTTGGATGCTTCAAGAGACCTTACAAAAGAAGATTAT
GAGATCTTTGATATTCTTTCGGGAAAGAATATAATATTTGTTCTCAACAAAGTAGATCTGCCTAAAAAAATTGATGAAGA
GGAGTTAAAAAAATTAGTAGGAAATGGTATAATAGTAGAGGTTTCTACTGTAGAAAGGACAGGACTTGACAAGCTGGAAA
GCGAGATATACAATTTGGTATTCAAAGGGAAGGTTTCTGCTACGGAGGAAGAGATAATTACAAATGCAAGGCACAGGGAA
GTGCTAATAAACGCAAAAAAACACATGGAAAGCGTCATAGAGGCAATTGAAAAAGGTTATAGCGAAGATTTAATAACGAT
TGATGTAAATGGAGCGCTAAATGAGATAGGAAAGATTACAGGTGAGACGGCAACAGAGGACGTGATAAATCAGATCTTTG
AAAGATTCTGTGTAGGAAAGTAG

Upstream 100 bases:

>100_bases
CCTTTTGCGGGGTCTTTTTTATTTTTTCTTGATTGAAAAGATTGAAAAATTGGTATATTATTTTATGTGATTGTGAAGTT
AGCTGAGGCTGGTGATGGAA

Downstream 100 bases:

>100_bases
GTGGAGGTATGAGGTATATTGCAGGAGAATATGATGTATGCGTTGTTGGATTGGGACATGCGGGAAGCGAAGCAGCTCTC
GCTTCTGCAAGGCTGGGACT

Product: tRNA modification GTPase TrmE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 460; Mature: 460

Protein sequence:

>460_residues
MEFDTIAAISTFPGEAGIGIVRISGDEALEIISKIFRPFRKKDIKSVKSHTIHYGHIVDPETGEVYDEVLVTVMRKPNTY
TREDVVEINCHGGIVVSSKILELVLKHGARLAEPGEFTKRAFLNGRIDLSQAEAVIDIITSKTMLANRYAQKQLAGVLGQ
KMKDLKNKIMELLSHLLALIDFPEEDVEELEREEIKRRAKDILNDIEYLIASSESGRIIREGLKTAIIGKPNVGKSSLLN
ALLKQNRAIVTDIPGTTRDVIEEYMNIKGIPIKLIDTAGIRHTDELVEKIGVEKSKEVLAEADLILFVLDASRDLTKEDY
EIFDILSGKNIIFVLNKVDLPKKIDEEELKKLVGNGIIVEVSTVERTGLDKLESEIYNLVFKGKVSATEEEIITNARHRE
VLINAKKHMESVIEAIEKGYSEDLITIDVNGALNEIGKITGETATEDVINQIFERFCVGK

Sequences:

>Translated_460_residues
MEFDTIAAISTFPGEAGIGIVRISGDEALEIISKIFRPFRKKDIKSVKSHTIHYGHIVDPETGEVYDEVLVTVMRKPNTY
TREDVVEINCHGGIVVSSKILELVLKHGARLAEPGEFTKRAFLNGRIDLSQAEAVIDIITSKTMLANRYAQKQLAGVLGQ
KMKDLKNKIMELLSHLLALIDFPEEDVEELEREEIKRRAKDILNDIEYLIASSESGRIIREGLKTAIIGKPNVGKSSLLN
ALLKQNRAIVTDIPGTTRDVIEEYMNIKGIPIKLIDTAGIRHTDELVEKIGVEKSKEVLAEADLILFVLDASRDLTKEDY
EIFDILSGKNIIFVLNKVDLPKKIDEEELKKLVGNGIIVEVSTVERTGLDKLESEIYNLVFKGKVSATEEEIITNARHRE
VLINAKKHMESVIEAIEKGYSEDLITIDVNGALNEIGKITGETATEDVINQIFERFCVGK
>Mature_460_residues
MEFDTIAAISTFPGEAGIGIVRISGDEALEIISKIFRPFRKKDIKSVKSHTIHYGHIVDPETGEVYDEVLVTVMRKPNTY
TREDVVEINCHGGIVVSSKILELVLKHGARLAEPGEFTKRAFLNGRIDLSQAEAVIDIITSKTMLANRYAQKQLAGVLGQ
KMKDLKNKIMELLSHLLALIDFPEEDVEELEREEIKRRAKDILNDIEYLIASSESGRIIREGLKTAIIGKPNVGKSSLLN
ALLKQNRAIVTDIPGTTRDVIEEYMNIKGIPIKLIDTAGIRHTDELVEKIGVEKSKEVLAEADLILFVLDASRDLTKEDY
EIFDILSGKNIIFVLNKVDLPKKIDEEELKKLVGNGIIVEVSTVERTGLDKLESEIYNLVFKGKVSATEEEIITNARHRE
VLINAKKHMESVIEAIEKGYSEDLITIDVNGALNEIGKITGETATEDVINQIFERFCVGK

Specific function: Exhibits a very high intrinsic GTPase hydrolysis rate. Involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA- cmnm(5)s(2)U34

COG id: COG0486

COG function: function code R; Predicted GTPase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 G (guanine nucleotide-binding) domain

Homologues:

Organism=Homo sapiens, GI193082991, Length=475, Percent_Identity=32.4210526315789, Blast_Score=219, Evalue=3e-57,
Organism=Homo sapiens, GI193082988, Length=505, Percent_Identity=30.6930693069307, Blast_Score=204, Evalue=1e-52,
Organism=Homo sapiens, GI193082993, Length=464, Percent_Identity=31.4655172413793, Blast_Score=203, Evalue=2e-52,
Organism=Escherichia coli, GI2367268, Length=468, Percent_Identity=34.8290598290598, Blast_Score=261, Evalue=6e-71,
Organism=Escherichia coli, GI87082120, Length=131, Percent_Identity=38.9312977099237, Blast_Score=89, Evalue=8e-19,
Organism=Caenorhabditis elegans, GI17507259, Length=471, Percent_Identity=28.6624203821656, Blast_Score=182, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6323665, Length=500, Percent_Identity=30.6, Blast_Score=197, Evalue=3e-51,
Organism=Drosophila melanogaster, GI45550813, Length=487, Percent_Identity=30.5954825462012, Blast_Score=216, Evalue=3e-56,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MNME_THETN (Q8R6K8)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_624296.1
- ProteinModelPortal:   Q8R6K8
- SMR:   Q8R6K8
- GeneID:   996096
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE2796
- NMPDR:   fig|273068.3.peg.2406
- HOGENOM:   HBG746795
- OMA:   KGPNSFT
- ProtClustDB:   PRK05291
- BioCyc:   TTEN273068:TTE2796-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00379
- InterPro:   IPR018948
- InterPro:   IPR006073
- InterPro:   IPR002917
- InterPro:   IPR005225
- InterPro:   IPR004520
- Gene3D:   G3DSA:3.30.1360.120
- PRINTS:   PR00326
- TIGRFAMs:   TIGR00450
- TIGRFAMs:   TIGR00231

Pfam domain/function: PF01926 MMR_HSR1; PF10396 TrmE_N

EC number: NA

Molecular weight: Translated: 51370; Mature: 51370

Theoretical pI: Translated: 4.98; Mature: 4.98

Prosite motif: NA

Important sites: BINDING 22-22 BINDING 87-87 BINDING 126-126 BINDING 460-460

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEFDTIAAISTFPGEAGIGIVRISGDEALEIISKIFRPFRKKDIKSVKSHTIHYGHIVDP
CCCCCEEHEECCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCEEECEECCC
ETGEVYDEVLVTVMRKPNTYTREDVVEINCHGGIVVSSKILELVLKHGARLAEPGEFTKR
CCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEHHHHHHHHHHCCCCCCCCCHHHHH
AFLNGRIDLSQAEAVIDIITSKTMLANRYAQKQLAGVLGQKMKDLKNKIMELLSHLLALI
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DFPEEDVEELEREEIKRRAKDILNDIEYLIASSESGRIIREGLKTAIIGKPNVGKSSLLN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCHHHEECCCCCCCHHHHHH
ALLKQNRAIVTDIPGTTRDVIEEYMNIKGIPIKLIDTAGIRHTDELVEKIGVEKSKEVLA
HHHHCCCEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHH
EADLILFVLDASRDLTKEDYEIFDILSGKNIIFVLNKVDLPKKIDEEELKKLVGNGIIVE
HCCEEEEEEECCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHCCCEEEE
VSTVERTGLDKLESEIYNLVFKGKVSATEEEIITNARHREVLINAKKHMESVIEAIEKGY
EECCHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCC
SEDLITIDVNGALNEIGKITGETATEDVINQIFERFCVGK
CCCEEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEFDTIAAISTFPGEAGIGIVRISGDEALEIISKIFRPFRKKDIKSVKSHTIHYGHIVDP
CCCCCEEHEECCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCEEECEECCC
ETGEVYDEVLVTVMRKPNTYTREDVVEINCHGGIVVSSKILELVLKHGARLAEPGEFTKR
CCHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCEEEHHHHHHHHHHCCCCCCCCCHHHHH
AFLNGRIDLSQAEAVIDIITSKTMLANRYAQKQLAGVLGQKMKDLKNKIMELLSHLLALI
HHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
DFPEEDVEELEREEIKRRAKDILNDIEYLIASSESGRIIREGLKTAIIGKPNVGKSSLLN
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCHHHEECCCCCCCHHHHHH
ALLKQNRAIVTDIPGTTRDVIEEYMNIKGIPIKLIDTAGIRHTDELVEKIGVEKSKEVLA
HHHHCCCEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHCCCHHHHHHH
EADLILFVLDASRDLTKEDYEIFDILSGKNIIFVLNKVDLPKKIDEEELKKLVGNGIIVE
HCCEEEEEEECCCCCCHHHHHHHHHCCCCCEEEEEECCCCCCCCCHHHHHHHHCCCEEEE
VSTVERTGLDKLESEIYNLVFKGKVSATEEEIITNARHREVLINAKKHMESVIEAIEKGY
EECCHHCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCC
SEDLITIDVNGALNEIGKITGETATEDVINQIFERFCVGK
CCCEEEEECCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336