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The map label for this gene is GidA2 [H]

Identifier: 20809124

GI number: 20809124

Start: 2683942

End: 2685843

Strand: Reverse

Name: GidA2 [H]

Synonym: TTE2795

Alternate gene names: 20809124

Gene position: 2685843-2683942 (Counterclockwise)

Preceding gene: 20809125

Following gene: 20809123

Centisome position: 99.87

GC content: 40.9

Gene sequence:

>1902_bases
ATGAGGTATATTGCAGGAGAATATGATGTATGCGTTGTTGGATTGGGACATGCGGGAAGCGAAGCAGCTCTCGCTTCTGC
AAGGCTGGGACTTGCAACGGTTGGCTTTGCGACAAATTTAGATGCTATAGCTCTTATGGCCTGCAATCCATCTATAGGTG
GCCCTGCTAAGGCACAGTTGGTAAGGGAAATAGATGCTTTAGGGGGAGAAATGGCTGTAAATACAGACAAATCTCTCCTT
CAAATGAGGACTTTAAATACCAGCAAGGGTCCTGCTGTCAGGTCTTTGAGAGCACAGGTGGACAAGAAACTGTATCAGGC
GAATATGAAGCACACTTTAGAAAGGCAGAAAAATCTGGATATAAAGCAGGCGGAGATTGTAGATATTTTAGTGGAAAATA
ATAAGGTAGTAGGTGTTGTGACAAAACTTGGAGCTATTTATAAATGTAAAGCCTGCATAATAACTACAGGAACTTTTTTG
AGAGGCAGAGTCATAATAGGAGAGGTTGGATTTGAAAGCGGTCCAAGTGGCCTTTTCCCGGCGAAGGAGTTGTCAGAAGC
TATAAAGAGATTGGGTTTTAAAATGATGAGATTCAATACGTCCACTCCTCCCAGAGTTGATAAAAGAACTGTCGATTTTT
CTAAGATGATTATGCAACCTGGCGATGAGGTTATAACTCCATTTTCTTTCATGCATGATAAGATTGAGATAGAGCAGATT
CCCTGCTGGCTCACCTACACCAATGAAAAAACTCACAAAATAATAAGGGATAATATACACAGAGCTCCTCTGTACACCGG
AGAAGTAGAAGGAGTGGGAGTTAGGTATTGTCCTTCCATTGAGGATAAAGTGATGAAATTTCCCCACAGAGACAGGCATC
AGATTTTTGTGGAGCCAGAGGGAAGAGATACTTACGAAATGTACATTCAGGGATTGTTTTCTAGCTTCCCTGAAGACCTT
CAGATGGAAATTCTAAGTACTATTCCTGGACTTGAGAATGCGAAGATTATGAGGCCAGCCTATGCGATAGAATACGACTG
TATAGATCCTACTCAGCTTAAAGCTACGCTTGAGACAAAGTTGGTAGAAGGATTGTATTTTGCAGGACAGGTGAATGGAA
CTTCTGGCTACGAAGAGGCAGCAGCACAAGGGCTCATGGCTGGCATAAATGCAGCTTTAAAAATCTTAGGAAAACCGCCT
CTAATTCTAGATAGGTCTCAGGCTTATATAGGAATTTTGATAGACGATTTAGTTACAAAAGGTACAAATGAGCCTTACAG
GATGCTTACTTCAAGAGCTGAGTACAGATTGATTTTAAGGCAGGACAATGCGGACTTCAGATTGACTGAGATAGGAAAAG
AAATAGGGCTTGTGACGGAAGAAAGGTATGAGAAATTTTTGAGGAAGAAAATACAGTTAGAAAAAGAGATGATGAGACTC
CCCACTGTCATGGTGAGGCCGACAGAAGAAGTAAACAACTTTTTAATTTCAAGGGGAAGTACTCCTTTAGTTTCAGGAGT
TGACCTGTACACCTTATTGAAAAGGCCTGAAATTGACTACAAATCCACTAAATTTTTGGACCCAACAAGGCCTGATGACA
TTTTGGATAGCGTTGCGGAGCAGATAGACATAAACATAAAATACGAGGGCTATATTTTAAAACAGTTAAGGCAGGTAGAG
CAGTTTAAAGCAATGGAGAATAAAAAAATCCCAGAGGACATAGATTACTATCAAGTTCATGGGCTCAGCAATGAAGCAAA
AGAGAAACTTTCAAAGATAAGGCCTACTTCTGTTGGACAGGCATCCCGTATTTCAGGGGTTTCTCCTGCTGATATTTCTG
TGCTTTTAATATACCTACAACAGATGAGGAGAAAGAGGTCCGATGAAGCAAAAATCAATTGA

Upstream 100 bases:

>100_bases
GAGCGCTAAATGAGATAGGAAAGATTACAGGTGAGACGGCAACAGAGGACGTGATAAATCAGATCTTTGAAAGATTCTGT
GTAGGAAAGTAGGTGGAGGT

Downstream 100 bases:

>100_bases
AATGCTAGTACAAGGAGCGAGTAGTTTAGGGATTACTTTGGAAATGTTCCATGTGGAACATTTCCAAAAATTTTATTCTC
TTTTATTGGAATGGAATCAA

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A 1 [H]

Number of amino acids: Translated: 633; Mature: 633

Protein sequence:

>633_residues
MRYIAGEYDVCVVGLGHAGSEAALASARLGLATVGFATNLDAIALMACNPSIGGPAKAQLVREIDALGGEMAVNTDKSLL
QMRTLNTSKGPAVRSLRAQVDKKLYQANMKHTLERQKNLDIKQAEIVDILVENNKVVGVVTKLGAIYKCKACIITTGTFL
RGRVIIGEVGFESGPSGLFPAKELSEAIKRLGFKMMRFNTSTPPRVDKRTVDFSKMIMQPGDEVITPFSFMHDKIEIEQI
PCWLTYTNEKTHKIIRDNIHRAPLYTGEVEGVGVRYCPSIEDKVMKFPHRDRHQIFVEPEGRDTYEMYIQGLFSSFPEDL
QMEILSTIPGLENAKIMRPAYAIEYDCIDPTQLKATLETKLVEGLYFAGQVNGTSGYEEAAAQGLMAGINAALKILGKPP
LILDRSQAYIGILIDDLVTKGTNEPYRMLTSRAEYRLILRQDNADFRLTEIGKEIGLVTEERYEKFLRKKIQLEKEMMRL
PTVMVRPTEEVNNFLISRGSTPLVSGVDLYTLLKRPEIDYKSTKFLDPTRPDDILDSVAEQIDINIKYEGYILKQLRQVE
QFKAMENKKIPEDIDYYQVHGLSNEAKEKLSKIRPTSVGQASRISGVSPADISVLLIYLQQMRRKRSDEAKIN

Sequences:

>Translated_633_residues
MRYIAGEYDVCVVGLGHAGSEAALASARLGLATVGFATNLDAIALMACNPSIGGPAKAQLVREIDALGGEMAVNTDKSLL
QMRTLNTSKGPAVRSLRAQVDKKLYQANMKHTLERQKNLDIKQAEIVDILVENNKVVGVVTKLGAIYKCKACIITTGTFL
RGRVIIGEVGFESGPSGLFPAKELSEAIKRLGFKMMRFNTSTPPRVDKRTVDFSKMIMQPGDEVITPFSFMHDKIEIEQI
PCWLTYTNEKTHKIIRDNIHRAPLYTGEVEGVGVRYCPSIEDKVMKFPHRDRHQIFVEPEGRDTYEMYIQGLFSSFPEDL
QMEILSTIPGLENAKIMRPAYAIEYDCIDPTQLKATLETKLVEGLYFAGQVNGTSGYEEAAAQGLMAGINAALKILGKPP
LILDRSQAYIGILIDDLVTKGTNEPYRMLTSRAEYRLILRQDNADFRLTEIGKEIGLVTEERYEKFLRKKIQLEKEMMRL
PTVMVRPTEEVNNFLISRGSTPLVSGVDLYTLLKRPEIDYKSTKFLDPTRPDDILDSVAEQIDINIKYEGYILKQLRQVE
QFKAMENKKIPEDIDYYQVHGLSNEAKEKLSKIRPTSVGQASRISGVSPADISVLLIYLQQMRRKRSDEAKIN
>Mature_633_residues
MRYIAGEYDVCVVGLGHAGSEAALASARLGLATVGFATNLDAIALMACNPSIGGPAKAQLVREIDALGGEMAVNTDKSLL
QMRTLNTSKGPAVRSLRAQVDKKLYQANMKHTLERQKNLDIKQAEIVDILVENNKVVGVVTKLGAIYKCKACIITTGTFL
RGRVIIGEVGFESGPSGLFPAKELSEAIKRLGFKMMRFNTSTPPRVDKRTVDFSKMIMQPGDEVITPFSFMHDKIEIEQI
PCWLTYTNEKTHKIIRDNIHRAPLYTGEVEGVGVRYCPSIEDKVMKFPHRDRHQIFVEPEGRDTYEMYIQGLFSSFPEDL
QMEILSTIPGLENAKIMRPAYAIEYDCIDPTQLKATLETKLVEGLYFAGQVNGTSGYEEAAAQGLMAGINAALKILGKPP
LILDRSQAYIGILIDDLVTKGTNEPYRMLTSRAEYRLILRQDNADFRLTEIGKEIGLVTEERYEKFLRKKIQLEKEMMRL
PTVMVRPTEEVNNFLISRGSTPLVSGVDLYTLLKRPEIDYKSTKFLDPTRPDDILDSVAEQIDINIKYEGYILKQLRQVE
QFKAMENKKIPEDIDYYQVHGLSNEAKEKLSKIRPTSVGQASRISGVSPADISVLLIYLQQMRRKRSDEAKIN

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 [H]

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family [H]

Homologues:

Organism=Homo sapiens, GI74024895, Length=638, Percent_Identity=40.9090909090909, Blast_Score=457, Evalue=1e-128,
Organism=Homo sapiens, GI19882217, Length=663, Percent_Identity=39.3665158371041, Blast_Score=444, Evalue=1e-124,
Organism=Homo sapiens, GI183227703, Length=678, Percent_Identity=38.4955752212389, Blast_Score=438, Evalue=1e-123,
Organism=Escherichia coli, GI2367273, Length=615, Percent_Identity=50.5691056910569, Blast_Score=632, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17534255, Length=627, Percent_Identity=39.2344497607655, Blast_Score=451, Evalue=1e-127,
Organism=Saccharomyces cerevisiae, GI6321202, Length=617, Percent_Identity=41.4910858995138, Blast_Score=469, Evalue=1e-133,
Organism=Drosophila melanogaster, GI24658174, Length=642, Percent_Identity=41.588785046729, Blast_Score=457, Evalue=1e-128,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: NA

Molecular weight: Translated: 71063; Mature: 71063

Theoretical pI: Translated: 8.02; Mature: 8.02

Prosite motif: PS01280 GIDA_1 ; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRYIAGEYDVCVVGLGHAGSEAALASARLGLATVGFATNLDAIALMACNPSIGGPAKAQL
CCCCCCCCCEEEEECCCCCCHHHHHHHHHCCEEECCCCCCCEEEEEEECCCCCCHHHHHH
VREIDALGGEMAVNTDKSLLQMRTLNTSKGPAVRSLRAQVDKKLYQANMKHTLERQKNLD
HHHHHHCCCCEEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IKQAEIVDILVENNKVVGVVTKLGAIYKCKACIITTGTFLRGRVIIGEVGFESGPSGLFP
CCHHHEEEEEEECCEEEEEHHHHCHHHCCCEEEEECCCHHCCEEEEEECCCCCCCCCCCC
AKELSEAIKRLGFKMMRFNTSTPPRVDKRTVDFSKMIMQPGDEVITPFSFMHDKIEIEQI
HHHHHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHCCCCHHCCCHHHHHCEEEEEEC
PCWLTYTNEKTHKIIRDNIHRAPLYTGEVEGVGVRYCPSIEDKVMKFPHRDRHQIFVEPE
CEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCEEECCCHHHHHHHCCCCCCCEEEEECC
GRDTYEMYIQGLFSSFPEDLQMEILSTIPGLENAKIMRPAYAIEYDCIDPTQLKATLETK
CCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEECCCEEEEECCCCHHHHHHHHHHH
LVEGLYFAGQVNGTSGYEEAAAQGLMAGINAALKILGKPPLILDRSQAYIGILIDDLVTK
HHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEHHHHHHHC
GTNEPYRMLTSRAEYRLILRQDNADFRLTEIGKEIGLVTEERYEKFLRKKIQLEKEMMRL
CCCCHHHHHHCCCCEEEEEEECCCCEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHC
PTVMVRPTEEVNNFLISRGSTPLVSGVDLYTLLKRPEIDYKSTKFLDPTRPDDILDSVAE
CCEEECCHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHH
QIDINIKYEGYILKQLRQVEQFKAMENKKIPEDIDYYQVHGLSNEAKEKLSKIRPTSVGQ
HEEEEEEECHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHCCCCCCCC
ASRISGVSPADISVLLIYLQQMRRKRSDEAKIN
HHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MRYIAGEYDVCVVGLGHAGSEAALASARLGLATVGFATNLDAIALMACNPSIGGPAKAQL
CCCCCCCCCEEEEECCCCCCHHHHHHHHHCCEEECCCCCCCEEEEEEECCCCCCHHHHHH
VREIDALGGEMAVNTDKSLLQMRTLNTSKGPAVRSLRAQVDKKLYQANMKHTLERQKNLD
HHHHHHCCCCEEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
IKQAEIVDILVENNKVVGVVTKLGAIYKCKACIITTGTFLRGRVIIGEVGFESGPSGLFP
CCHHHEEEEEEECCEEEEEHHHHCHHHCCCEEEEECCCHHCCEEEEEECCCCCCCCCCCC
AKELSEAIKRLGFKMMRFNTSTPPRVDKRTVDFSKMIMQPGDEVITPFSFMHDKIEIEQI
HHHHHHHHHHHCCEEEEECCCCCCCCCCCHHHHHHHHHCCCCHHCCCHHHHHCEEEEEEC
PCWLTYTNEKTHKIIRDNIHRAPLYTGEVEGVGVRYCPSIEDKVMKFPHRDRHQIFVEPE
CEEEEECCCHHHHHHHHCCCCCCCCCCCCCCCCEEECCCHHHHHHHCCCCCCCEEEEECC
GRDTYEMYIQGLFSSFPEDLQMEILSTIPGLENAKIMRPAYAIEYDCIDPTQLKATLETK
CCCHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCCEEECCCEEEEECCCCHHHHHHHHHHH
LVEGLYFAGQVNGTSGYEEAAAQGLMAGINAALKILGKPPLILDRSQAYIGILIDDLVTK
HHHHEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCEEEEHHHHHHHC
GTNEPYRMLTSRAEYRLILRQDNADFRLTEIGKEIGLVTEERYEKFLRKKIQLEKEMMRL
CCCCHHHHHHCCCCEEEEEEECCCCEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHC
PTVMVRPTEEVNNFLISRGSTPLVSGVDLYTLLKRPEIDYKSTKFLDPTRPDDILDSVAE
CCEEECCHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHH
QIDINIKYEGYILKQLRQVEQFKAMENKKIPEDIDYYQVHGLSNEAKEKLSKIRPTSVGQ
HEEEEEEECHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCHHHHHHHHHCCCCCCCC
ASRISGVSPADISVLLIYLQQMRRKRSDEAKIN
HHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]