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The map label for this gene is FruA [H]

Identifier: 20808934

GI number: 20808934

Start: 2470617

End: 2471975

Strand: Reverse

Name: FruA [H]

Synonym: TTE2585

Alternate gene names: 20808934

Gene position: 2471975-2470617 (Counterclockwise)

Preceding gene: 20808935

Following gene: 20808931

Centisome position: 91.91

GC content: 41.43

Gene sequence:

>1359_bases
ATGAAGAAAATTGTTGCCGTAACAGCTTGTCCTACAGGTATTGCACATACCTATATGGCGGCGGAGAATCTGCAGATGGC
AGCAAAAGAGATGGGAGTTGACATAAAAGTAGAGACTCAAGGGTCAATAGGTGCAGAAAATGCTCTTACAGAAGAGGACA
TAAGAGAAGCGGATGCTGTTATAATTGCTGCTTCCACTAAGGTGGACAAATCAAGATTTGCTGGGAAACCTGTTCTGGAA
GTTGGCGTGGAAGATGCTATAAAAGATGCAAAAGGGCTTATTGAAAAAGCTTTAAAGATGGAGAAACCTACTGACTATGT
GGAGAAAGTACAGGAGATTCACAAAGAAAGAGCTGCTCAGAGGACAGGAGCCTACAAGCACCTCATGACAGGTGTTTCCT
ACATGATACCTTTTGTAGTTGCAGGCGGTATTTTAATTGCGCTTTCTTTCTTCTGGGGATATAAGGCGTTTGAAGTAGAA
GGAACCTTACCATGGGCTTTGATGAGAATTGGCGGTGGTTCAGCATTTGCGCTGATGGTACCAATTTTGTCAGGGTATAT
AGCATTTTCTATAGCAGATAGACCTGGCCTTGTTCCTGGTATGGTAGGTGGTATGCTGGCAGTATCAACAGGTGCAGGAT
TTTTAGGCGGAATCATTTCAGGATTTTTGGCAGGGTATACGGTAGACTATTTGAAGAAGTTAATAAAACTGCCTAGGACT
TTAGAAGGTTTAATGCCAGTATTGATATTGCCGGTATTGTCTACTTTAATAGTAGGACTTTTAATGATATATGTGATTGG
TTCACCAATGAAAGCTATTATGACATCTTTGACTAACTGGCTTACAGGGATGAGCTCTACAAATGCGGTTATTTTCAGAG
CAATATTAGGACTTATGATGGCTTTTGATATGGGTGGTCCTGTTAACAAGACAGCTTATACCTTTGCAACAGGGCTTTTG
GGCTCAAATGTGTTTACTCCGATGGCAGCAGTTATGGCAGCTGGTATGACACCGCCACTTGGCCTTGCACTTGCAACAGT
TTTATTTAAAGATAGATTTACAAAAGATGAAATAGAAGCCGGTAAAGCAGCTTGGGTATTGGGAGCCTCCTTTATAACAG
AAGGGGCTATACCTTTTGCAGCAGCAGACCCATTTAGGGTAATACCTTCTATAATGGTGGGTTCTGCAGTTACAGGCGCA
CTTTCAATGCTTTTCCACATTGAATTAAGGGCTCCTCACGGAGGTATATTTGTGATACCTATAGCTGTTTCAAATCCACT
TCTTTATATTGGTGTGATAGCAGTCGGTACAGTTGTAACTGCCTTAATGGTTGCACTTCTTAAAAAGAAAGTAGAATAA

Upstream 100 bases:

>100_bases
TTGATGCATAAGGAGGTGAGGGAAGCGCTGGAGAAGGCTTCTACACCGGAGGAAGTTATAAGAGCTTTTGAAAATTAAAC
TTTAAGAGAGGGGAGTAATC

Downstream 100 bases:

>100_bases
AAAATCAGGGCAAGCTCTTGTTTGCCCTGACTTTTTATTTGTTAAAAGGATTTTGCACTCTTACTTTACCAAAGTACTGC
CCTGAATTTAAATCCTCGCT

Product: phosphotransferase system, fructose-specific IIC component

Products: NA

Alternate protein names: EIIBBC-Fru; Fructose-specific phosphotransferase enzyme IIB component; EIII-Fru; PTS system fructose-specific EIIB component; Fructose permease IIC component; PTS system fructose-specific EIIC component [H]

Number of amino acids: Translated: 452; Mature: 452

Protein sequence:

>452_residues
MKKIVAVTACPTGIAHTYMAAENLQMAAKEMGVDIKVETQGSIGAENALTEEDIREADAVIIAASTKVDKSRFAGKPVLE
VGVEDAIKDAKGLIEKALKMEKPTDYVEKVQEIHKERAAQRTGAYKHLMTGVSYMIPFVVAGGILIALSFFWGYKAFEVE
GTLPWALMRIGGGSAFALMVPILSGYIAFSIADRPGLVPGMVGGMLAVSTGAGFLGGIISGFLAGYTVDYLKKLIKLPRT
LEGLMPVLILPVLSTLIVGLLMIYVIGSPMKAIMTSLTNWLTGMSSTNAVIFRAILGLMMAFDMGGPVNKTAYTFATGLL
GSNVFTPMAAVMAAGMTPPLGLALATVLFKDRFTKDEIEAGKAAWVLGASFITEGAIPFAAADPFRVIPSIMVGSAVTGA
LSMLFHIELRAPHGGIFVIPIAVSNPLLYIGVIAVGTVVTALMVALLKKKVE

Sequences:

>Translated_452_residues
MKKIVAVTACPTGIAHTYMAAENLQMAAKEMGVDIKVETQGSIGAENALTEEDIREADAVIIAASTKVDKSRFAGKPVLE
VGVEDAIKDAKGLIEKALKMEKPTDYVEKVQEIHKERAAQRTGAYKHLMTGVSYMIPFVVAGGILIALSFFWGYKAFEVE
GTLPWALMRIGGGSAFALMVPILSGYIAFSIADRPGLVPGMVGGMLAVSTGAGFLGGIISGFLAGYTVDYLKKLIKLPRT
LEGLMPVLILPVLSTLIVGLLMIYVIGSPMKAIMTSLTNWLTGMSSTNAVIFRAILGLMMAFDMGGPVNKTAYTFATGLL
GSNVFTPMAAVMAAGMTPPLGLALATVLFKDRFTKDEIEAGKAAWVLGASFITEGAIPFAAADPFRVIPSIMVGSAVTGA
LSMLFHIELRAPHGGIFVIPIAVSNPLLYIGVIAVGTVVTALMVALLKKKVE
>Mature_452_residues
MKKIVAVTACPTGIAHTYMAAENLQMAAKEMGVDIKVETQGSIGAENALTEEDIREADAVIIAASTKVDKSRFAGKPVLE
VGVEDAIKDAKGLIEKALKMEKPTDYVEKVQEIHKERAAQRTGAYKHLMTGVSYMIPFVVAGGILIALSFFWGYKAFEVE
GTLPWALMRIGGGSAFALMVPILSGYIAFSIADRPGLVPGMVGGMLAVSTGAGFLGGIISGFLAGYTVDYLKKLIKLPRT
LEGLMPVLILPVLSTLIVGLLMIYVIGSPMKAIMTSLTNWLTGMSSTNAVIFRAILGLMMAFDMGGPVNKTAYTFATGLL
GSNVFTPMAAVMAAGMTPPLGLALATVLFKDRFTKDEIEAGKAAWVLGASFITEGAIPFAAADPFRVIPSIMVGSAVTGA
LSMLFHIELRAPHGGIFVIPIAVSNPLLYIGVIAVGTVVTALMVALLKKKVE

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1299

COG function: function code G; Phosphotransferase system, fructose-specific IIC component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788492, Length=450, Percent_Identity=55.7777777777778, Blast_Score=491, Evalue=1e-140,
Organism=Escherichia coli, GI1786951, Length=428, Percent_Identity=41.588785046729, Blast_Score=316, Evalue=2e-87,
Organism=Escherichia coli, GI87082348, Length=473, Percent_Identity=36.3636363636364, Blast_Score=259, Evalue=3e-70,
Organism=Escherichia coli, GI1790386, Length=333, Percent_Identity=44.7447447447448, Blast_Score=241, Evalue=7e-65,
Organism=Escherichia coli, GI1788729, Length=385, Percent_Identity=28.5714285714286, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1790387, Length=97, Percent_Identity=54.639175257732, Blast_Score=104, Evalue=1e-23,
Organism=Escherichia coli, GI1788730, Length=100, Percent_Identity=47, Blast_Score=92, Evalue=5e-20,
Organism=Escherichia coli, GI1790390, Length=62, Percent_Identity=50, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR003353
- InterPro:   IPR006327 [H]

Pfam domain/function: PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 47669; Mature: 47669

Theoretical pI: Translated: 9.08; Mature: 9.08

Prosite motif: PS51099 PTS_EIIB_TYPE_2 ; PS51104 PTS_EIIC_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
5.5 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKIVAVTACPTGIAHTYMAAENLQMAAKEMGVDIKVETQGSIGAENALTEEDIREADAV
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCHHHHHHHCEE
IIAASTKVDKSRFAGKPVLEVGVEDAIKDAKGLIEKALKMEKPTDYVEKVQEIHKERAAQ
EEEECCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
RTGAYKHLMTGVSYMIPFVVAGGILIALSFFWGYKAFEVEGTLPWALMRIGGGSAFALMV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEHEEECCCCHHHHHH
PILSGYIAFSIADRPGLVPGMVGGMLAVSTGAGFLGGIISGFLAGYTVDYLKKLIKLPRT
HHHHHHHEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHH
LEGLMPVLILPVLSTLIVGLLMIYVIGSPMKAIMTSLTNWLTGMSSTNAVIFRAILGLMM
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
AFDMGGPVNKTAYTFATGLLGSNVFTPMAAVMAAGMTPPLGLALATVLFKDRFTKDEIEA
HHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHC
GKAAWVLGASFITEGAIPFAAADPFRVIPSIMVGSAVTGALSMLFHIELRAPHGGIFVIP
CCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEE
IAVSNPLLYIGVIAVGTVVTALMVALLKKKVE
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKKIVAVTACPTGIAHTYMAAENLQMAAKEMGVDIKVETQGSIGAENALTEEDIREADAV
CCCEEEEEECCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCCCCHHHHHHHCEE
IIAASTKVDKSRFAGKPVLEVGVEDAIKDAKGLIEKALKMEKPTDYVEKVQEIHKERAAQ
EEEECCCCHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
RTGAYKHLMTGVSYMIPFVVAGGILIALSFFWGYKAFEVEGTLPWALMRIGGGSAFALMV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCEEHEEECCCCHHHHHH
PILSGYIAFSIADRPGLVPGMVGGMLAVSTGAGFLGGIISGFLAGYTVDYLKKLIKLPRT
HHHHHHHEEEECCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCHHH
LEGLMPVLILPVLSTLIVGLLMIYVIGSPMKAIMTSLTNWLTGMSSTNAVIFRAILGLMM
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHH
AFDMGGPVNKTAYTFATGLLGSNVFTPMAAVMAAGMTPPLGLALATVLFKDRFTKDEIEA
HHCCCCCCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCHHHHHC
GKAAWVLGASFITEGAIPFAAADPFRVIPSIMVGSAVTGALSMLFHIELRAPHGGIFVIP
CCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCEEEEE
IAVSNPLLYIGVIAVGTVVTALMVALLKKKVE
EECCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2254279 [H]