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The map label for this gene is PtsN3 [H]

Identifier: 20808935

GI number: 20808935

Start: 2471998

End: 2472453

Strand: Reverse

Name: PtsN3 [H]

Synonym: TTE2586

Alternate gene names: 20808935

Gene position: 2472453-2471998 (Counterclockwise)

Preceding gene: 20808936

Following gene: 20808934

Centisome position: 91.93

GC content: 37.5

Gene sequence:

>456_bases
GTGAGGGAAATGGAGATAAAAGATGTGCTTAATGAAAAGATGATGGTTTTTGATTTGAAAGCGAAAACAAAGGAGGAAGT
CCTAGAAGAGTTAGTGAAAATTTTAAAGGAAAATGATGTGGTAGATGATGAAAAAGGTTTTTTAGAGGTTGTTAAGAAAA
GAGAAGAGGAATTTTCTACAGGAATAGGCTATGGAGTAGCAATACCTCACGGAAAGAGTAGTCTTGTGAAAAAGCCTTCA
ATCGTGTTTGGCAAATCCCAAAATGGGATTGATTATAATTCAATGGATGGTAAGCCCGCTCATCTTTTTTTCCTAATTGC
TGTACCAGACAATTCAAATGAGCTACACCTAAAAGTTTTGGCTGATCTTTCAAGGGCTTTGATGCATAAGGAGGTGAGGG
AAGCGCTGGAGAAGGCTTCTACACCGGAGGAAGTTATAAGAGCTTTTGAAAATTAA

Upstream 100 bases:

>100_bases
CGTTGTCAACTTCTGTGGTCATGAAAGAGGGTACAGGAGCGGGTACTATTGAAGAAGTAAATGCATTAAAAGACAAAATT
GAGATTGAGAGGATAGAAAG

Downstream 100 bases:

>100_bases
ACTTTAAGAGAGGGGAGTAATCATGAAGAAAATTGTTGCCGTAACAGCTTGTCCTACAGGTATTGCACATACCTATATGG
CGGCGGAGAATCTGCAGATG

Product: phosphotransferase system mannitol/fructose-specific IIA domain-containing protein

Products: NA

Alternate protein names: EIIABC-Fru; Fructose-specific phosphotransferase enzyme IIA component; EII-Fru; PTS system fructose-specific EIIA component; Fructose-specific phosphotransferase enzyme IIB component; EIII-Fru; PTS system fructose-specific EIIB component; Fructose permease IIC component; PTS system fructose-specific EIIC component [H]

Number of amino acids: Translated: 151; Mature: 151

Protein sequence:

>151_residues
MREMEIKDVLNEKMMVFDLKAKTKEEVLEELVKILKENDVVDDEKGFLEVVKKREEEFSTGIGYGVAIPHGKSSLVKKPS
IVFGKSQNGIDYNSMDGKPAHLFFLIAVPDNSNELHLKVLADLSRALMHKEVREALEKASTPEEVIRAFEN

Sequences:

>Translated_151_residues
MREMEIKDVLNEKMMVFDLKAKTKEEVLEELVKILKENDVVDDEKGFLEVVKKREEEFSTGIGYGVAIPHGKSSLVKKPS
IVFGKSQNGIDYNSMDGKPAHLFFLIAVPDNSNELHLKVLADLSRALMHKEVREALEKASTPEEVIRAFEN
>Mature_151_residues
MREMEIKDVLNEKMMVFDLKAKTKEEVLEELVKILKENDVVDDEKGFLEVVKKREEEFSTGIGYGVAIPHGKSSLVKKPS
IVFGKSQNGIDYNSMDGKPAHLFFLIAVPDNSNELHLKVLADLSRALMHKEVREALEKASTPEEVIRAFEN

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIC type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI2367327, Length=134, Percent_Identity=31.3432835820896, Blast_Score=96, Evalue=1e-21,
Organism=Escherichia coli, GI1786951, Length=150, Percent_Identity=26.6666666666667, Blast_Score=75, Evalue=2e-15,
Organism=Escherichia coli, GI1788726, Length=148, Percent_Identity=28.3783783783784, Blast_Score=65, Evalue=2e-12,
Organism=Escherichia coli, GI1789597, Length=143, Percent_Identity=27.972027972028, Blast_Score=63, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR013011
- InterPro:   IPR003501
- InterPro:   IPR003352
- InterPro:   IPR013014
- InterPro:   IPR004715
- InterPro:   IPR003353
- InterPro:   IPR006327 [H]

Pfam domain/function: PF00359 PTS_EIIA_2; PF02378 PTS_EIIC; PF02302 PTS_IIB [H]

EC number: =2.7.1.69 [H]

Molecular weight: Translated: 17102; Mature: 17102

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MREMEIKDVLNEKMMVFDLKAKTKEEVLEELVKILKENDVVDDEKGFLEVVKKREEEFST
CCCCHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHC
GIGYGVAIPHGKSSLVKKPSIVFGKSQNGIDYNSMDGKPAHLFFLIAVPDNSNELHLKVL
CCCCEEECCCCCHHHHCCCCEEECCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCHHHHH
ADLSRALMHKEVREALEKASTPEEVIRAFEN
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHCC
>Mature Secondary Structure
MREMEIKDVLNEKMMVFDLKAKTKEEVLEELVKILKENDVVDDEKGFLEVVKKREEEFST
CCCCHHHHHHCCCEEEEECCCCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHC
GIGYGVAIPHGKSSLVKKPSIVFGKSQNGIDYNSMDGKPAHLFFLIAVPDNSNELHLKVL
CCCCEEECCCCCHHHHCCCCEEECCCCCCCCCCCCCCCCEEEEEEEEECCCCCCCHHHHH
ADLSRALMHKEVREALEKASTPEEVIRAFEN
HHHHHHHHHHHHHHHHHHCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]