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The map label for this gene is truA

Identifier: 20808627

GI number: 20808627

Start: 2157862

End: 2158584

Strand: Reverse

Name: truA

Synonym: TTE2258

Alternate gene names: 20808627

Gene position: 2158584-2157862 (Counterclockwise)

Preceding gene: 20808628

Following gene: 20808626

Centisome position: 80.26

GC content: 39.14

Gene sequence:

>723_bases
ATGCTTATCATTGAATATGACGGGACCAATTATCACGGCTGGCAGTACCAAAAGAATGCCATAACTGTTCAGGAAGTGCT
TGAAAAAGCTATAGAAAAAGTAACAGGAGAAAAAGTTAACCTAATAGGAAGCAGCAGAACAGATACTGGTGTTCATGCTT
TGTACCAAGTTGCCAATTTTAAGACGAAAACAAAGATCCCGCCAGAAAAACTTCCTTACGCTTTAAATGCTGTACTGCCT
GATGATGTAGTTGTAGTCAAAGCAAAAGAAGTTGACAAATCATTTCACGCTCGTTACAGTGCAAGAAGTAAAAGATACAG
GTACTTGATTTTAAATAGACAGTTTCCAATGCCTACTATGAGAAATTATTGCTGGCATGTCCCATACCCCTTAGATGTTG
AGGCGATGAAGAAAGCGGCTTTCTTTTTTAGAGGGACCCATGACTTTTCTGCTTTTAAAGCCTCAGGAAACAAAAAGGTG
AATCCTGTAAAAACTATCTTTGATTTGACGGTAGAAAAAGAAGGGGATCTGATTAAAATAGAGGTAGAAGCAGATGGATT
CTTATACAACATGGTAAGAATAATAGTGGGAACGCTAGTGTACGTGGGACTTGGCAAAATAAAGGCAGGAGAAATTCCAG
CTATTATAGAATCAAAAGACAGGACAAAGGCCGGCATAACAGCTCCTCCTCAAGGCCTATACCTTGTCAGGATTGTCTAT
TGA

Upstream 100 bases:

>100_bases
GAAGTGCACACAAGAGACTATGCAGCTCTTTTCATAACAGGTGTAATTGTGGTGCTAATTGTGTGGAACAGAAGTTGGGC
GTGGTAGTATGAAGAACATA

Downstream 100 bases:

>100_bases
CAGTTGACATACTGTGAATTAAAATAATATTATGCGAAATTGTGACCAGAATAAGGAGGTTGTAGAGCAAAATGAAATCC
TATATGGCTAAACCCAGTGA

Product: tRNA pseudouridine synthase A

Products: NA

Alternate protein names: tRNA pseudouridylate synthase I; tRNA-uridine isomerase I

Number of amino acids: Translated: 240; Mature: 240

Protein sequence:

>240_residues
MLIIEYDGTNYHGWQYQKNAITVQEVLEKAIEKVTGEKVNLIGSSRTDTGVHALYQVANFKTKTKIPPEKLPYALNAVLP
DDVVVVKAKEVDKSFHARYSARSKRYRYLILNRQFPMPTMRNYCWHVPYPLDVEAMKKAAFFFRGTHDFSAFKASGNKKV
NPVKTIFDLTVEKEGDLIKIEVEADGFLYNMVRIIVGTLVYVGLGKIKAGEIPAIIESKDRTKAGITAPPQGLYLVRIVY

Sequences:

>Translated_240_residues
MLIIEYDGTNYHGWQYQKNAITVQEVLEKAIEKVTGEKVNLIGSSRTDTGVHALYQVANFKTKTKIPPEKLPYALNAVLP
DDVVVVKAKEVDKSFHARYSARSKRYRYLILNRQFPMPTMRNYCWHVPYPLDVEAMKKAAFFFRGTHDFSAFKASGNKKV
NPVKTIFDLTVEKEGDLIKIEVEADGFLYNMVRIIVGTLVYVGLGKIKAGEIPAIIESKDRTKAGITAPPQGLYLVRIVY
>Mature_240_residues
MLIIEYDGTNYHGWQYQKNAITVQEVLEKAIEKVTGEKVNLIGSSRTDTGVHALYQVANFKTKTKIPPEKLPYALNAVLP
DDVVVVKAKEVDKSFHARYSARSKRYRYLILNRQFPMPTMRNYCWHVPYPLDVEAMKKAAFFFRGTHDFSAFKASGNKKV
NPVKTIFDLTVEKEGDLIKIEVEADGFLYNMVRIIVGTLVYVGLGKIKAGEIPAIIESKDRTKAGITAPPQGLYLVRIVY

Specific function: Formation of pseudouridine at positions 38, 39 and 40 in the anticodon stem and loop of transfer RNAs

COG id: COG0101

COG function: function code J; Pseudouridylate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA pseudouridine synthase truA family

Homologues:

Organism=Homo sapiens, GI23503259, Length=270, Percent_Identity=34.8148148148148, Blast_Score=137, Evalue=6e-33,
Organism=Homo sapiens, GI199559785, Length=254, Percent_Identity=27.9527559055118, Blast_Score=73, Evalue=3e-13,
Organism=Escherichia coli, GI1788657, Length=239, Percent_Identity=38.0753138075314, Blast_Score=174, Evalue=5e-45,
Organism=Drosophila melanogaster, GI116007752, Length=300, Percent_Identity=27, Blast_Score=80, Evalue=8e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUA_THETN (Q8R7Y7)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_623798.1
- ProteinModelPortal:   Q8R7Y7
- SMR:   Q8R7Y7
- GeneID:   995484
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE2258
- NMPDR:   fig|273068.3.peg.2439
- HOGENOM:   HBG621020
- OMA:   QSKTPFR
- ProtClustDB:   PRK00021
- BioCyc:   TTEN273068:TTE2258-MONOMER
- BRENDA:   5.4.99.12
- HAMAP:   MF_00171
- InterPro:   IPR020103
- InterPro:   IPR001406
- InterPro:   IPR020097
- InterPro:   IPR020095
- InterPro:   IPR020094
- Gene3D:   G3DSA:3.30.70.580
- Gene3D:   G3DSA:3.30.70.660
- PANTHER:   PTHR11142
- PIRSF:   PIRSF001430
- TIGRFAMs:   TIGR00071

Pfam domain/function: PF01416 PseudoU_synth_1; SSF55120 PsdUridine_synth_cat_dom

EC number: =5.4.99.12

Molecular weight: Translated: 27193; Mature: 27193

Theoretical pI: Translated: 9.90; Mature: 9.90

Prosite motif: NA

Important sites: ACT_SITE 48-48

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIIEYDGTNYHGWQYQKNAITVQEVLEKAIEKVTGEKVNLIGSSRTDTGVHALYQVANF
CEEEEECCCCCCCEEEECCCEEHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCC
KTKTKIPPEKLPYALNAVLPDDVVVVKAKEVDKSFHARYSARSKRYRYLILNRQFPMPTM
CCCCCCCHHHCCEEEECCCCCCEEEEEHHHHCHHHHHHHHHCCCCEEEEEEECCCCCCHH
RNYCWHVPYPLDVEAMKKAAFFFRGTHDFSAFKASGNKKVNPVKTIFDLTVEKEGDLIKI
HHCEEECCCCCCHHHHHHHHHHEECCCCCHHEECCCCCCCCHHHEEEEEEEECCCCEEEE
EVEADGFLYNMVRIIVGTLVYVGLGKIKAGEIPAIIESKDRTKAGITAPPQGLYLVRIVY
EECCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCEEECCCCCCCCCCCCCCCEEEEEEEC
>Mature Secondary Structure
MLIIEYDGTNYHGWQYQKNAITVQEVLEKAIEKVTGEKVNLIGSSRTDTGVHALYQVANF
CEEEEECCCCCCCEEEECCCEEHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCC
KTKTKIPPEKLPYALNAVLPDDVVVVKAKEVDKSFHARYSARSKRYRYLILNRQFPMPTM
CCCCCCCHHHCCEEEECCCCCCEEEEEHHHHCHHHHHHHHHCCCCEEEEEEECCCCCCHH
RNYCWHVPYPLDVEAMKKAAFFFRGTHDFSAFKASGNKKVNPVKTIFDLTVEKEGDLIKI
HHCEEECCCCCCHHHHHHHHHHEECCCCCHHEECCCCCCCCHHHEEEEEEEECCCCEEEE
EVEADGFLYNMVRIIVGTLVYVGLGKIKAGEIPAIIESKDRTKAGITAPPQGLYLVRIVY
EECCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCEEECCCCCCCCCCCCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336