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The map label for this gene is ThiM

Identifier: 20808607

GI number: 20808607

Start: 2137173

End: 2137976

Strand: Reverse

Name: ThiM

Synonym: TTE2232

Alternate gene names: 20808607

Gene position: 2137976-2137173 (Counterclockwise)

Preceding gene: 20808608

Following gene: 20808606

Centisome position: 79.5

GC content: 41.92

Gene sequence:

>804_bases
ATGGGGAGTGAGGTTTTGAAGCTTTTAAGAGACAAAAAACCTCTTATACATCATATTACAAATGTGGTTACAGTAAATGA
CTGTGCTAATGTAACTTTGGCTATAGGGGCATTGCCTGTCATGGCATATGCCCCTGAAGAAGTAGAAGAAATGGTGAAAA
ATGCCAATGCTCTTGTTCTAAATATAGGCACTTTAAGCGTGGAAGAAGTTAATACCATGATAAAAGCAGGTAAAACGGCA
AACCAGCACGGCGTACCTGTGATTCTTGACCCGGTTGGAGCCGGAGCAACAAGTTTGAGGACTAAAAGCGCTAAGAGGAT
TATTGAGGAAGTCAGGGTTAGTGTGATAAAGGGAAATAGCGCAGAAATTTCTACTTTAAGTGGGAGAACAGGAAAAATAA
GGGGAGTTGAAGCGGTAGAGGAAGAGGAAGATATATCCGATATTGCTTATGACCTTGCAGTAAAGTGTGGATGTGTCGTT
GCAGTATCAGGAAAAGTGGATGTCATAACTGATGGAAAGAAAATTGCCTATGTAAAAAATGGGCATCCTATGATGGGGAC
AATAACAGGTACAGGGTGCATGCTCACATCAGTGGTGGCGAGCTTTTGTGCAGTGGCTGAGGATTATTTTGATGGAACTT
TGGAGGCCTTTGTCGCATTTGGAATTGCAGGAGAGAAGGCGGCGCAAAATCCCAAGGTTGAAGGCCCGGGAAGTTTTAAA
GTAGCCTTTTTTGATGAGATATACAATTTAAGTCCTGGAGTTTTGGCATCAGAGAGGAAAATTGACTTGATTGTAAAAGG
ATGA

Upstream 100 bases:

>100_bases
ATATCTGCAATAGCAGGTATTTTTGATGGAGATATTGAGGCTAATACGAAAGATATGTTAAGGGAAATAAGAAGAGCTTT
TAAAGAGAGGGGGAAATAAT

Downstream 100 bases:

>100_bases
TATAATTTTGTCAGATTATGTCGAAGATTGTTAAAGAGGGGGTGGCAGGTGAGAGGCAGCTAAATTATTACAAAAGACAA
AAGCCAGAAGAAGGGGAGGC

Product: hydroxyethylthiazole kinase

Products: NA

Alternate protein names: 4-methyl-5-beta-hydroxyethylthiazole kinase; TH kinase; Thz kinase

Number of amino acids: Translated: 267; Mature: 266

Protein sequence:

>267_residues
MGSEVLKLLRDKKPLIHHITNVVTVNDCANVTLAIGALPVMAYAPEEVEEMVKNANALVLNIGTLSVEEVNTMIKAGKTA
NQHGVPVILDPVGAGATSLRTKSAKRIIEEVRVSVIKGNSAEISTLSGRTGKIRGVEAVEEEEDISDIAYDLAVKCGCVV
AVSGKVDVITDGKKIAYVKNGHPMMGTITGTGCMLTSVVASFCAVAEDYFDGTLEAFVAFGIAGEKAAQNPKVEGPGSFK
VAFFDEIYNLSPGVLASERKIDLIVKG

Sequences:

>Translated_267_residues
MGSEVLKLLRDKKPLIHHITNVVTVNDCANVTLAIGALPVMAYAPEEVEEMVKNANALVLNIGTLSVEEVNTMIKAGKTA
NQHGVPVILDPVGAGATSLRTKSAKRIIEEVRVSVIKGNSAEISTLSGRTGKIRGVEAVEEEEDISDIAYDLAVKCGCVV
AVSGKVDVITDGKKIAYVKNGHPMMGTITGTGCMLTSVVASFCAVAEDYFDGTLEAFVAFGIAGEKAAQNPKVEGPGSFK
VAFFDEIYNLSPGVLASERKIDLIVKG
>Mature_266_residues
GSEVLKLLRDKKPLIHHITNVVTVNDCANVTLAIGALPVMAYAPEEVEEMVKNANALVLNIGTLSVEEVNTMIKAGKTAN
QHGVPVILDPVGAGATSLRTKSAKRIIEEVRVSVIKGNSAEISTLSGRTGKIRGVEAVEEEEDISDIAYDLAVKCGCVVA
VSGKVDVITDGKKIAYVKNGHPMMGTITGTGCMLTSVVASFCAVAEDYFDGTLEAFVAFGIAGEKAAQNPKVEGPGSFKV
AFFDEIYNLSPGVLASERKIDLIVKG

Specific function: Thiamine biosynthesis. [C]

COG id: COG2145

COG function: function code H; Hydroxyethylthiazole kinase, sugar kinase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Thz kinase family

Homologues:

Organism=Escherichia coli, GI1788421, Length=254, Percent_Identity=34.6456692913386, Blast_Score=149, Evalue=3e-37,
Organism=Saccharomyces cerevisiae, GI6325042, Length=275, Percent_Identity=30.9090909090909, Blast_Score=108, Evalue=1e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIM_THETN (Q8R807)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_623778.1
- ProteinModelPortal:   Q8R807
- SMR:   Q8R807
- GeneID:   997939
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE2232
- NMPDR:   fig|273068.3.peg.2419
- HOGENOM:   HBG351126
- OMA:   AIRGNAG
- ProtClustDB:   PRK09355
- BioCyc:   TTEN273068:TTE2232-MONOMER
- BRENDA:   2.7.1.50
- HAMAP:   MF_00228
- InterPro:   IPR000417
- PANTHER:   PTHR20857:SF14
- PIRSF:   PIRSF000513
- PRINTS:   PR01099
- TIGRFAMs:   TIGR00694

Pfam domain/function: PF02110 HK

EC number: =2.7.1.50

Molecular weight: Translated: 28107; Mature: 27976

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: NA

Important sites: BINDING 41-41 BINDING 117-117 BINDING 163-163 BINDING 190-190

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGSEVLKLLRDKKPLIHHITNVVTVNDCANVTLAIGALPVMAYAPEEVEEMVKNANALVL
CCHHHHHHHHCCCHHHHHHHHEEEECCCCCEEEEEHHHHHHHCCHHHHHHHHHCCCEEEE
NIGTLSVEEVNTMIKAGKTANQHGVPVILDPVGAGATSLRTKSAKRIIEEVRVSVIKGNS
EECCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHEEEECCC
AEISTLSGRTGKIRGVEAVEEEEDISDIAYDLAVKCGCVVAVSGKVDVITDGKKIAYVKN
CEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEECCCEEEEEEC
GHPMMGTITGTGCMLTSVVASFCAVAEDYFDGTLEAFVAFGIAGEKAAQNPKVEGPGSFK
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCEE
VAFFDEIYNLSPGVLASERKIDLIVKG
EEEEHHHHCCCCCCEECCCEEEEEEEC
>Mature Secondary Structure 
GSEVLKLLRDKKPLIHHITNVVTVNDCANVTLAIGALPVMAYAPEEVEEMVKNANALVL
CHHHHHHHHCCCHHHHHHHHEEEECCCCCEEEEEHHHHHHHCCHHHHHHHHHCCCEEEE
NIGTLSVEEVNTMIKAGKTANQHGVPVILDPVGAGATSLRTKSAKRIIEEVRVSVIKGNS
EECCCCHHHHHHHHHCCCCCCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHEEEECCC
AEISTLSGRTGKIRGVEAVEEEEDISDIAYDLAVKCGCVVAVSGKVDVITDGKKIAYVKN
CEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCCEEEEECCCEEEEEEC
GHPMMGTITGTGCMLTSVVASFCAVAEDYFDGTLEAFVAFGIAGEKAAQNPKVEGPGSFK
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHCCCCCCCCCCCEE
VAFFDEIYNLSPGVLASERKIDLIVKG
EEEEHHHHCCCCCCEECCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336