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The map label for this gene is thiE

Identifier: 20808608

GI number: 20808608

Start: 2137978

End: 2138565

Strand: Reverse

Name: thiE

Synonym: TTE2233

Alternate gene names: 20808608

Gene position: 2138565-2137978 (Counterclockwise)

Preceding gene: 20808609

Following gene: 20808607

Centisome position: 79.52

GC content: 39.12

Gene sequence:

>588_bases
ATGGACATAGCTGAAGCGGTGGAGATAGCTATTAAAAATGGTGTTACTGTGGTTCAGCTGAGGGAAAAAGATATATCCAG
CAGAGAATTCTATGAAATTGCCCTTAAAGTAAAGGAAGTTACAAGAAAATATAATGTTCCTCTAATTATAAATGACAGAG
TTGATATAGCCCTGGCTGTAGATGCCGAAGGTGTACATGTAGGGCCTGATGATTTGCCTGTTGGCGTTGTGAGGAGAATA
TTGGGACCTGACAAAATAGTGGGAGGGTCTGCGTATTCCGTTGAAGAAGCTTTAAAAGCAGAAAAAGAAGGAGCTGACTA
CATAGGAGCAGGATCCGTATTTGCACAGCCTGTAAAACCTGAAGCAGAGGTCATAGGTATAGAAGGAGTTAGAAAAATAA
AAGAAGCTGTAAATATACCTGTGGTAGCCATAGGAGGGGTAAATAAAACTAATGCTTATGAAGTGATTTTGCATTCAGGA
GTAGATGGGATATCTGCAATAGCAGGTATTTTTGATGGAGATATTGAGGCTAATACGAAAGATATGTTAAGGGAAATAAG
AAGAGCTTTTAAAGAGAGGGGGAAATAA

Upstream 100 bases:

>100_bases
TAGATGGCGGCCTTGTGATGGATTGAATAAGGGGGTAAAGGTTGTGAAGAGGATAGACTTTACTTTGTATGCTATCACTG
ACAGGTCCTTTATAAAAGGG

Downstream 100 bases:

>100_bases
TATGGGGAGTGAGGTTTTGAAGCTTTTAAGAGACAAAAAACCTCTTATACATCATATTACAAATGTGGTTACAGTAAATG
ACTGTGCTAATGTAACTTTG

Product: thiamine-phosphate pyrophosphorylase

Products: NA

Alternate protein names: TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase

Number of amino acids: Translated: 195; Mature: 195

Protein sequence:

>195_residues
MDIAEAVEIAIKNGVTVVQLREKDISSREFYEIALKVKEVTRKYNVPLIINDRVDIALAVDAEGVHVGPDDLPVGVVRRI
LGPDKIVGGSAYSVEEALKAEKEGADYIGAGSVFAQPVKPEAEVIGIEGVRKIKEAVNIPVVAIGGVNKTNAYEVILHSG
VDGISAIAGIFDGDIEANTKDMLREIRRAFKERGK

Sequences:

>Translated_195_residues
MDIAEAVEIAIKNGVTVVQLREKDISSREFYEIALKVKEVTRKYNVPLIINDRVDIALAVDAEGVHVGPDDLPVGVVRRI
LGPDKIVGGSAYSVEEALKAEKEGADYIGAGSVFAQPVKPEAEVIGIEGVRKIKEAVNIPVVAIGGVNKTNAYEVILHSG
VDGISAIAGIFDGDIEANTKDMLREIRRAFKERGK
>Mature_195_residues
MDIAEAVEIAIKNGVTVVQLREKDISSREFYEIALKVKEVTRKYNVPLIINDRVDIALAVDAEGVHVGPDDLPVGVVRRI
LGPDKIVGGSAYSVEEALKAEKEGADYIGAGSVFAQPVKPEAEVIGIEGVRKIKEAVNIPVVAIGGVNKTNAYEVILHSG
VDGISAIAGIFDGDIEANTKDMLREIRRAFKERGK

Specific function: Condenses 4-methyl-5-(beta-hydroxyethyl)thiazole monophosphate (THZ-P) and 2-methyl-4-amino-5-hydroxymethyl pyrimidine pyrophosphate (HMP-PP) to form thiamine monophosphate (TMP)

COG id: COG0352

COG function: function code H; Thiamine monophosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TMP-PPase family

Homologues:

Organism=Saccharomyces cerevisiae, GI6325042, Length=212, Percent_Identity=40.0943396226415, Blast_Score=140, Evalue=1e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIE_THETN (Q8R806)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_623779.1
- ProteinModelPortal:   Q8R806
- GeneID:   997949
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE2233
- NMPDR:   fig|273068.3.peg.2420
- HOGENOM:   HBG754477
- OMA:   SAICHAE
- ProtClustDB:   PRK00043
- BioCyc:   TTEN273068:TTE2233-MONOMER
- BRENDA:   2.5.1.3
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF02581 TMP-TENI; SSF51391 TMP_synthase

EC number: =2.5.1.3

Molecular weight: Translated: 20960; Mature: 20960

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDIAEAVEIAIKNGVTVVQLREKDISSREFYEIALKVKEVTRKYNVPLIINDRVDIALAV
CCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEE
DAEGVHVGPDDLPVGVVRRILGPDKIVGGSAYSVEEALKAEKEGADYIGAGSVFAQPVKP
CCCCCEECCCCCCHHHHHHHCCCCCEECCCCHHHHHHHHHHHCCCCEECCCCCCCCCCCC
EAEVIGIEGVRKIKEAVNIPVVAIGGVNKTNAYEVILHSGVDGISAIAGIFDGDIEANTK
CCEEECHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCHH
DMLREIRRAFKERGK
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MDIAEAVEIAIKNGVTVVQLREKDISSREFYEIALKVKEVTRKYNVPLIINDRVDIALAV
CCHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEE
DAEGVHVGPDDLPVGVVRRILGPDKIVGGSAYSVEEALKAEKEGADYIGAGSVFAQPVKP
CCCCCEECCCCCCHHHHHHHCCCCCEECCCCHHHHHHHHHHHCCCCEECCCCCCCCCCCC
EAEVIGIEGVRKIKEAVNIPVVAIGGVNKTNAYEVILHSGVDGISAIAGIFDGDIEANTK
CCEEECHHHHHHHHHHHCCCEEEECCCCCCCCEEEEEECCCCHHHHHHHHHCCCCCCCHH
DMLREIRRAFKERGK
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336