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The map label for this gene is RpoE5 [H]

Identifier: 20808562

GI number: 20808562

Start: 2094127

End: 2094657

Strand: Direct

Name: RpoE5 [H]

Synonym: TTE2178

Alternate gene names: 20808562

Gene position: 2094127-2094657 (Clockwise)

Preceding gene: 20808559

Following gene: 20808563

Centisome position: 77.86

GC content: 30.89

Gene sequence:

>531_bases
ATGGAAGAGAAAGAATTGATATATAAAGCCAAAAATGGCAATAAACACGCTCTAAATGAACTCCTCTTACAAAACTATAA
TACTTTACTGGGATATTGCATTAAAATGACAGGCAATCCTACACTTGCCCAAGATATTGCACAGGATACAATGCTTAAAG
CTATTTTAAATATTAAAAAATTTGCCCCTGAAGCTAAATTCTCTACATGGCTTTTAAAAATTGCCACTAATACATTTAAA
GATTATTTAAGAAAACATAAAAATGTTGAACTTGTTGAAGAAATGTCTACAAATAACACGGAAAACATAGAAGAAACCGC
TATTTCAAACCTTCAATATAAGGAACTAATGAACATTTTAATGAAACTTCCTTATGAGAAAAGAGCAACTTTTATTTTAA
AACACTATTATGGTTACAAATACGAAGAAATTGCACAAATACTAGATTGTCCTGTAGGAACGGTGCGTTCGCGTTTACAC
AATGCTATAAAAGAGATAATGAAGGAAATGGAAAGAAGGGATTTGCTGTGA

Upstream 100 bases:

>100_bases
ACACTATAAAAATTAATTTCCAAGGATTTATTTTTGATAGAATAAATTCGTGAACTTTTTTCTTTTTTTAGCGTTTAATT
TAAGGAAAGGTGGGCAGGTG

Downstream 100 bases:

>100_bases
AAAAAAAGTGTAAAGAAATACAAAACAAAATAATCGATATGCATTACGGTGAAATTGACGAAAATATAGAAATTATTTCA
CATATTGAAAATTGCAATGA

Product: RNA polymerase sigma factor SigY

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 176; Mature: 176

Protein sequence:

>176_residues
MEEKELIYKAKNGNKHALNELLLQNYNTLLGYCIKMTGNPTLAQDIAQDTMLKAILNIKKFAPEAKFSTWLLKIATNTFK
DYLRKHKNVELVEEMSTNNTENIEETAISNLQYKELMNILMKLPYEKRATFILKHYYGYKYEEIAQILDCPVGTVRSRLH
NAIKEIMKEMERRDLL

Sequences:

>Translated_176_residues
MEEKELIYKAKNGNKHALNELLLQNYNTLLGYCIKMTGNPTLAQDIAQDTMLKAILNIKKFAPEAKFSTWLLKIATNTFK
DYLRKHKNVELVEEMSTNNTENIEETAISNLQYKELMNILMKLPYEKRATFILKHYYGYKYEEIAQILDCPVGTVRSRLH
NAIKEIMKEMERRDLL
>Mature_176_residues
MEEKELIYKAKNGNKHALNELLLQNYNTLLGYCIKMTGNPTLAQDIAQDTMLKAILNIKKFAPEAKFSTWLLKIATNTFK
DYLRKHKNVELVEEMSTNNTENIEETAISNLQYKELMNILMKLPYEKRATFILKHYYGYKYEEIAQILDCPVGTVRSRLH
NAIKEIMKEMERRDLL

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. Positively regulates the expression of the sigY- yxlCDEFG operon upon nitrogen starvation. Also positively regulates ybg

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=178, Percent_Identity=30.8988764044944, Blast_Score=84, Evalue=5e-18,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 20653; Mature: 20653

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: PS01063 SIGMA70_ECF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEKELIYKAKNGNKHALNELLLQNYNTLLGYCIKMTGNPTLAQDIAQDTMLKAILNIKK
CCCHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
FAPEAKFSTWLLKIATNTFKDYLRKHKNVELVEEMSTNNTENIEETAISNLQYKELMNIL
HCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHH
MKLPYEKRATFILKHYYGYKYEEIAQILDCPVGTVRSRLHNAIKEIMKEMERRDLL
HHCCCHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEEKELIYKAKNGNKHALNELLLQNYNTLLGYCIKMTGNPTLAQDIAQDTMLKAILNIKK
CCCHHHEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
FAPEAKFSTWLLKIATNTFKDYLRKHKNVELVEEMSTNNTENIEETAISNLQYKELMNIL
HCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHCCCHHHHHHHH
MKLPYEKRATFILKHYYGYKYEEIAQILDCPVGTVRSRLHNAIKEIMKEMERRDLL
HHCCCHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969509; 9384377 [H]