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The map label for this gene is HisS2

Identifier: 20808530

GI number: 20808530

Start: 2055791

End: 2056930

Strand: Reverse

Name: HisS2

Synonym: TTE2140

Alternate gene names: 20808530

Gene position: 2056930-2055791 (Counterclockwise)

Preceding gene: 20808532

Following gene: 20808529

Centisome position: 76.48

GC content: 35.09

Gene sequence:

>1140_bases
ATGATATATTTGCCGGATGGAGTGCAGGACTTCCTTCCTGAAGAATATGAATTCAAGAGAAATATAGAGGACAAATTTAG
AGAGGTTTTCAAAAGTTTTGGCTATAAAGAGATAATGCCTCCCACTTTTGAGTACAGCGAAAATTTTTCTCATTTATTTG
ATGAAAACAGCATGTACAGGTTTTTTGATAAAAAGGGGAATATTTTAGCTTTAAGGCCTGATGTAACTGCTCAAATAGCC
AGAATTGTTTCTACAAAGTTGGAAGGCAGATACCCACTTAAGTTATGCTATGTAGCTAATGTGTATAGGTATGAGGACAC
ACAAGTAGGTAAAATGAGAGAATTTACACAGGCAGGAGTAGAGCTTATAGGTACAAATCACGAAGAATCAGATGCGGAGG
TTATAGCTCTTTCTATAGAGGCATTAAAAAGTACAGGCTTAAAGGATTTTAAAATTGATATAGGACATGCTGAAGTATTT
GGAAGTATTGTAAGAAATTTAAATCTAGGAAATGAAGATGTAAATCTATTGAGAGAACTTTTAGAGCAAAAAAACCAATC
TGCTATAGAAGATTTCATAAAACAAAAAGAAATAAAAAGAGAAGAGGCTAAACTTCTAAGGGAACTTCCTCTTTTATTCG
GTGGGGAGGAGATTTTAGAAAAGTTAAAAAAAGAAAATTTTAAAGAGACGGAGGGAGTGCTTGAGTATCTTGATAGAGTG
TATAAGATACTTGAGGACTTTGGTATGAAGGAATATATAAGTTTTGATTTGGGGATGGTGCAAAATCTAAATTATTACAC
AGGAATTATATTTAGAGGTTTTGTAAAAGGGCTTGGCTATGCTATATGCACTGGAGGAAGGTATGACAAGCTCCTTAAGA
TTTACGGTAAAGACTTGCCTGCTACAGGCTTTGCAATAAGTGTAGAAAGAGTTATGCTTGCTTTGCAAAGGCAAAGCAAG
GGAGAATGGGTAAAACCCCGCAGAGTGCTAGTAAGGTATAAAGAAGAAGAGAGAAGAAGAGCTTATGAAAAGGCTAATGT
ACTCAGAAAAGAAGGCAATGTCGTAGAGATGTACACTTTTAAGAGGTGTGAAAATATTGATTTAAAAAGTTTTGATGAAG
TCGTAGATGTGGGGGAATGA

Upstream 100 bases:

>100_bases
TTGTCTCTTCTTGACATACATATAAAATTAATATACAATAAAAGAGATACAATTCATTACTTTATCACGTTACAAAGACA
TAGCGATGTGAGGGATTTAA

Downstream 100 bases:

>100_bases
CATGGATATTGTTTCAATAGCTTTGCCAAAGGGCAGGATGACAGAGGATGCAGTAGTTTTATTTAAAAAAGCAGGTATTT
CTAATGATGTGTTAAAAGAT

Product: histidyl-tRNA synthetase

Products: AMP; diphosphate +L-histidyl-tRNAHis [C]

Alternate protein names: NA

Number of amino acids: Translated: 379; Mature: 379

Protein sequence:

>379_residues
MIYLPDGVQDFLPEEYEFKRNIEDKFREVFKSFGYKEIMPPTFEYSENFSHLFDENSMYRFFDKKGNILALRPDVTAQIA
RIVSTKLEGRYPLKLCYVANVYRYEDTQVGKMREFTQAGVELIGTNHEESDAEVIALSIEALKSTGLKDFKIDIGHAEVF
GSIVRNLNLGNEDVNLLRELLEQKNQSAIEDFIKQKEIKREEAKLLRELPLLFGGEEILEKLKKENFKETEGVLEYLDRV
YKILEDFGMKEYISFDLGMVQNLNYYTGIIFRGFVKGLGYAICTGGRYDKLLKIYGKDLPATGFAISVERVMLALQRQSK
GEWVKPRRVLVRYKEEERRRAYEKANVLRKEGNVVEMYTFKRCENIDLKSFDEVVDVGE

Sequences:

>Translated_379_residues
MIYLPDGVQDFLPEEYEFKRNIEDKFREVFKSFGYKEIMPPTFEYSENFSHLFDENSMYRFFDKKGNILALRPDVTAQIA
RIVSTKLEGRYPLKLCYVANVYRYEDTQVGKMREFTQAGVELIGTNHEESDAEVIALSIEALKSTGLKDFKIDIGHAEVF
GSIVRNLNLGNEDVNLLRELLEQKNQSAIEDFIKQKEIKREEAKLLRELPLLFGGEEILEKLKKENFKETEGVLEYLDRV
YKILEDFGMKEYISFDLGMVQNLNYYTGIIFRGFVKGLGYAICTGGRYDKLLKIYGKDLPATGFAISVERVMLALQRQSK
GEWVKPRRVLVRYKEEERRRAYEKANVLRKEGNVVEMYTFKRCENIDLKSFDEVVDVGE
>Mature_379_residues
MIYLPDGVQDFLPEEYEFKRNIEDKFREVFKSFGYKEIMPPTFEYSENFSHLFDENSMYRFFDKKGNILALRPDVTAQIA
RIVSTKLEGRYPLKLCYVANVYRYEDTQVGKMREFTQAGVELIGTNHEESDAEVIALSIEALKSTGLKDFKIDIGHAEVF
GSIVRNLNLGNEDVNLLRELLEQKNQSAIEDFIKQKEIKREEAKLLRELPLLFGGEEILEKLKKENFKETEGVLEYLDRV
YKILEDFGMKEYISFDLGMVQNLNYYTGIIFRGFVKGLGYAICTGGRYDKLLKIYGKDLPATGFAISVERVMLALQRQSK
GEWVKPRRVLVRYKEEERRRAYEKANVLRKEGNVVEMYTFKRCENIDLKSFDEVVDVGE

Specific function: Required for the first step of histidine biosynthesis. May allow the feedback regulation of ATP phosphoribosyltransferase activity by histidine

COG id: COG3705

COG function: function code E; ATP phosphoribosyltransferase involved in histidine biosynthesis

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. HisZ subfamily

Homologues:

Organism=Homo sapiens, GI15029520, Length=357, Percent_Identity=26.3305322128852, Blast_Score=111, Evalue=1e-24,
Organism=Homo sapiens, GI6996014, Length=336, Percent_Identity=26.1904761904762, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1788861, Length=329, Percent_Identity=28.5714285714286, Blast_Score=114, Evalue=1e-26,
Organism=Caenorhabditis elegans, GI71993693, Length=401, Percent_Identity=21.9451371571072, Blast_Score=74, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI71993686, Length=401, Percent_Identity=21.9451371571072, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6325290, Length=374, Percent_Identity=27.0053475935829, Blast_Score=117, Evalue=3e-27,
Organism=Drosophila melanogaster, GI24643061, Length=362, Percent_Identity=26.2430939226519, Blast_Score=132, Evalue=4e-31,
Organism=Drosophila melanogaster, GI24643059, Length=381, Percent_Identity=25.7217847769029, Blast_Score=122, Evalue=4e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HISZ_THETN (Q8R880)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_623701.1
- ProteinModelPortal:   Q8R880
- SMR:   Q8R880
- GeneID:   997460
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE2140
- NMPDR:   fig|273068.3.peg.2244
- HOGENOM:   HBG616575
- OMA:   YREHVKS
- ProtClustDB:   CLSK901563
- BioCyc:   TTEN273068:TTE2140-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00125
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004517
- InterPro:   IPR004516
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00443

Pfam domain/function: PF00587 tRNA-synt_2b

EC number: 6.1.1.21 [C]

Molecular weight: Translated: 44209; Mature: 44209

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYLPDGVQDFLPEEYEFKRNIEDKFREVFKSFGYKEIMPPTFEYSENFSHLFDENSMYR
CEECCCCHHHHCCCHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCHHCCHHHHHCCCCHHH
FFDKKGNILALRPDVTAQIARIVSTKLEGRYPLKLCYVANVYRYEDTQVGKMREFTQAGV
HCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCEEEEEEHHHHCCCCCHHHHHHHHHHHHH
ELIGTNHEESDAEVIALSIEALKSTGLKDFKIDIGHAEVFGSIVRNLNLGNEDVNLLREL
HHHCCCCCCCCHHEEEHHHHHHHHCCCCEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHH
LEQKNQSAIEDFIKQKEIKREEAKLLRELPLLFGGEEILEKLKKENFKETEGVLEYLDRV
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHH
YKILEDFGMKEYISFDLGMVQNLNYYTGIIFRGFVKGLGYAICTGGRYDKLLKIYGKDLP
HHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHCCCCC
ATGFAISVERVMLALQRQSKGEWVKPRRVLVRYKEEERRRAYEKANVLRKEGNVVEMYTF
CCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEH
KRCENIDLKSFDEVVDVGE
HHCCCCCHHHHHHHHCCCC
>Mature Secondary Structure
MIYLPDGVQDFLPEEYEFKRNIEDKFREVFKSFGYKEIMPPTFEYSENFSHLFDENSMYR
CEECCCCHHHHCCCHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCHHCCHHHHHCCCCHHH
FFDKKGNILALRPDVTAQIARIVSTKLEGRYPLKLCYVANVYRYEDTQVGKMREFTQAGV
HCCCCCCEEEECCCHHHHHHHHHHHHCCCCCCEEEEEEHHHHCCCCCHHHHHHHHHHHHH
ELIGTNHEESDAEVIALSIEALKSTGLKDFKIDIGHAEVFGSIVRNLNLGNEDVNLLREL
HHHCCCCCCCCHHEEEHHHHHHHHCCCCEEEEECCHHHHHHHHHHHCCCCCHHHHHHHHH
LEQKNQSAIEDFIKQKEIKREEAKLLRELPLLFGGEEILEKLKKENFKETEGVLEYLDRV
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHH
YKILEDFGMKEYISFDLGMVQNLNYYTGIIFRGFVKGLGYAICTGGRYDKLLKIYGKDLP
HHHHHHCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHCCCCC
ATGFAISVERVMLALQRQSKGEWVKPRRVLVRYKEEERRRAYEKANVLRKEGNVVEMYTF
CCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEH
KRCENIDLKSFDEVVDVGE
HHCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Mg2+ [C]

Kcat value (1/min): 8520 [C]

Specific activity: 121.8

Km value (mM): 0.06 {His}} 0.046 {His}} 0.03 {His}} 0.02 {His}} 0.017 {His}} 0.011 {His}} 0.008 {His}} 0.0014 {tRNAHis}} 0.00056 {tRNAHis}} 0.0016 {U73tRNAHisGUG}} 0.00031 {U73tRNAHisGUG}} 0.89 {ATP}} 0.74 {ATP}} 0.655 {ATP}} 0.5

Substrates: ATP; @HIST01.txt*L-histidine!; tRNAHis [C]

Specific reaction: ATP + @HIST01.txt*L-histidine! + tRNAHis = AMP + diphosphate +L-histidyl-tRNAHis [C]

General reaction: Aminoacylation; Esterification [C]

Inhibitor: 1, 2, 4-Triazole-3-alanine; D-Histidine; Diphosphate; L-Histidinol; Mg2+; N-Acetyl histidine; NEM; p-Chloromercuribenzoate [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336