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The map label for this gene is RecJ2 [H]

Identifier: 20808502

GI number: 20808502

Start: 2029141

End: 2030616

Strand: Reverse

Name: RecJ2 [H]

Synonym: TTE2108

Alternate gene names: 20808502

Gene position: 2030616-2029141 (Counterclockwise)

Preceding gene: 20808503

Following gene: 20808501

Centisome position: 75.5

GC content: 38.21

Gene sequence:

>1476_bases
ATGCTCTGGAGATATAATCCAAATACAAAAATTGAACTGCGGAGGAAGTATCCTGTAACTCCCAGCGACTTCCCCCTTTC
TGACATTGTAAAAACTATTTTTGAAAGCAAAAGCATTGCCATTTTTGGAGATTATGACGTTGATGGAATTTCATCTTCAC
TTATACTCAAAAATGCTTTAGATGTTCTGGGAATCCAGAACAAAATATTTTTACCGGAAAGAGAAGAGGGATACGGAATA
AAACCTGAACACGTACAACAAATAAAAGCTGAAGGTTTTGATGCGGTTATTACTGTTGACAACGGTATAACAGCTTTTGA
AGCAGCAAAAGCAGCCAGAGAAGCAGGGATTAAGCTGATTATAACAGATCACCACGAACCTCTAGCACATCTTCCTGATG
CATATAAAATTTTTAATCCCAAGCTTTATAAAGACAAATATCAGGACTATGCAGGAGCAGGAGTGGCTTATCTAATTGCA
AGGGAACTTTTGAGACAGAGAAATTTTTCAGTAACTCCTGCTATGATACAGCTTGCAGGTCTTGCTACTGTTGCCGATGT
TGTACCTCTTGATGGCAACAACTGGTACATTGCAAGAAAAGGCCTTGAACTTATGAGGAAACAGCCCATAAAAGGCATAA
AAGAAATATTAAGGACTGCAGGAAGAAATCACGAAAATCTTACAGGCTTTGATATAGGATGGATAATAGCTCCTCGAATT
AATGCCACAGGGCGCTTAAAAAGCCCTAAAATGGGTTTGGAGCTTCTTCTTTACGGTACAAATGCTGAAGAAGTTGAACA
ATTGAACAGAAAGCGTTTAGAGCTTGTAAACCAGTATTTAAGTCGTATTGAAGACAGCGAAAACAAATTTTTAATATATG
CCTTTAAAAACTGCCCCAGAGGTATAACAGGACTTATCGCAGGGCGTGCTGCTGAGAAATTTCAGAAGCCTGTAATGGTG
AGCAGTATAGACAGCAGTGGTAAAGCAGTATCTTCAGCAAGAACTTACGGAGATTTTGATTTAATGGAGGCTTTTAAGTA
CGTATCTGAACATGTAGATATAACTTTTGGAGGACATAAATCAGCAGCAGGTGTTTCATACAGCATTAGAGATTTAAAAA
GGATCCAAAACTTATTGAACAAATACACGGAAGAAAATCCGCCTAAAGAAGAGATAAGAGACCTGGATGGTGTTTTAACG
AAAGTACCAGATTTAGAAGAGGTTATGGCTTTTGACAGCTTTGAGCCCTTTGGTTACAAAAATCCAGAGCCTGCTTTTTT
ACTGGAAGGCACAGTTACCGATGTGAGAGCAGATCAGGACTGGCAGCTGGTGATTGTAAACGGAGAATTTGGATTCTTTC
TGGATAATACTTATAGAAAGGGCGATAAAATAAAATTCATTGTAAGTCCGTATATAAAAAACGGTTATGTTAAGCTTTGG
GTGCTGGATGAGAAGCCTACAATTATAGATGAATAA

Upstream 100 bases:

>100_bases
ATAAAAATAGCTATAAACACAATTGACAGCTCAAAGAGGTATACGGCTCTAAAGGAGAGATTGAAAAACGTGAAGGAGTA
TGCTGAAAGGGGAATTTTAT

Downstream 100 bases:

>100_bases
AAACGAGAAAATCATCTTTGCTTTTACATTTATCAGAAACTATCAGTTACTGTCGTTGATGAAGAAAGGAGTATTTAATA
TAAAAGGAGCGTTCAATATG

Product: single-stranded DNA-specific exonuclease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MLWRYNPNTKIELRRKYPVTPSDFPLSDIVKTIFESKSIAIFGDYDVDGISSSLILKNALDVLGIQNKIFLPEREEGYGI
KPEHVQQIKAEGFDAVITVDNGITAFEAAKAAREAGIKLIITDHHEPLAHLPDAYKIFNPKLYKDKYQDYAGAGVAYLIA
RELLRQRNFSVTPAMIQLAGLATVADVVPLDGNNWYIARKGLELMRKQPIKGIKEILRTAGRNHENLTGFDIGWIIAPRI
NATGRLKSPKMGLELLLYGTNAEEVEQLNRKRLELVNQYLSRIEDSENKFLIYAFKNCPRGITGLIAGRAAEKFQKPVMV
SSIDSSGKAVSSARTYGDFDLMEAFKYVSEHVDITFGGHKSAAGVSYSIRDLKRIQNLLNKYTEENPPKEEIRDLDGVLT
KVPDLEEVMAFDSFEPFGYKNPEPAFLLEGTVTDVRADQDWQLVIVNGEFGFFLDNTYRKGDKIKFIVSPYIKNGYVKLW
VLDEKPTIIDE

Sequences:

>Translated_491_residues
MLWRYNPNTKIELRRKYPVTPSDFPLSDIVKTIFESKSIAIFGDYDVDGISSSLILKNALDVLGIQNKIFLPEREEGYGI
KPEHVQQIKAEGFDAVITVDNGITAFEAAKAAREAGIKLIITDHHEPLAHLPDAYKIFNPKLYKDKYQDYAGAGVAYLIA
RELLRQRNFSVTPAMIQLAGLATVADVVPLDGNNWYIARKGLELMRKQPIKGIKEILRTAGRNHENLTGFDIGWIIAPRI
NATGRLKSPKMGLELLLYGTNAEEVEQLNRKRLELVNQYLSRIEDSENKFLIYAFKNCPRGITGLIAGRAAEKFQKPVMV
SSIDSSGKAVSSARTYGDFDLMEAFKYVSEHVDITFGGHKSAAGVSYSIRDLKRIQNLLNKYTEENPPKEEIRDLDGVLT
KVPDLEEVMAFDSFEPFGYKNPEPAFLLEGTVTDVRADQDWQLVIVNGEFGFFLDNTYRKGDKIKFIVSPYIKNGYVKLW
VLDEKPTIIDE
>Mature_491_residues
MLWRYNPNTKIELRRKYPVTPSDFPLSDIVKTIFESKSIAIFGDYDVDGISSSLILKNALDVLGIQNKIFLPEREEGYGI
KPEHVQQIKAEGFDAVITVDNGITAFEAAKAAREAGIKLIITDHHEPLAHLPDAYKIFNPKLYKDKYQDYAGAGVAYLIA
RELLRQRNFSVTPAMIQLAGLATVADVVPLDGNNWYIARKGLELMRKQPIKGIKEILRTAGRNHENLTGFDIGWIIAPRI
NATGRLKSPKMGLELLLYGTNAEEVEQLNRKRLELVNQYLSRIEDSENKFLIYAFKNCPRGITGLIAGRAAEKFQKPVMV
SSIDSSGKAVSSARTYGDFDLMEAFKYVSEHVDITFGGHKSAAGVSYSIRDLKRIQNLLNKYTEENPPKEEIRDLDGVLT
KVPDLEEVMAFDSFEPFGYKNPEPAFLLEGTVTDVRADQDWQLVIVNGEFGFFLDNTYRKGDKIKFIVSPYIKNGYVKLW
VLDEKPTIIDE

Specific function: Putative single-stranded-DNA-specific exonuclease. RecA thread formation during DNA double-strand break repair requires RecJ or AadAB [H]

COG id: COG0608

COG function: function code L; Single-stranded DNA-specific exonuclease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 OB DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789259, Length=432, Percent_Identity=32.1759259259259, Blast_Score=188, Evalue=8e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003156
- InterPro:   IPR001667
- InterPro:   IPR004610
- InterPro:   IPR018779 [H]

Pfam domain/function: PF01368 DHH; PF02272 DHHA1; PF10141 ssDNA-exonuc_C [H]

EC number: 3.1.-.- [C]

Molecular weight: Translated: 55397; Mature: 55397

Theoretical pI: Translated: 6.43; Mature: 6.43

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLWRYNPNTKIELRRKYPVTPSDFPLSDIVKTIFESKSIAIFGDYDVDGISSSLILKNAL
CCEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHH
DVLGIQNKIFLPEREEGYGIKPEHVQQIKAEGFDAVITVDNGITAFEAAKAAREAGIKLI
HHHCCCCEEECCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCEEEE
ITDHHEPLAHLPDAYKIFNPKLYKDKYQDYAGAGVAYLIARELLRQRNFSVTPAMIQLAG
EECCCCCHHHCCCCHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHH
LATVADVVPLDGNNWYIARKGLELMRKQPIKGIKEILRTAGRNHENLTGFDIGWIIAPRI
HHHHHHEEECCCCCEEEECCCHHHHHHCCHHHHHHHHHHHCCCCCCCCCEEEEEEEECCC
NATGRLKSPKMGLELLLYGTNAEEVEQLNRKRLELVNQYLSRIEDSENKFLIYAFKNCPR
CCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCC
GITGLIAGRAAEKFQKPVMVSSIDSSGKAVSSARTYGDFDLMEAFKYVSEHVDITFGGHK
CHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHCCCCHHHHHHHHHHHHCEEEEECCCC
SAAGVSYSIRDLKRIQNLLNKYTEENPPKEEIRDLDGVLTKVPDLEEVMAFDSFEPFGYK
CCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCC
NPEPAFLLEGTVTDVRADQDWQLVIVNGEFGFFLDNTYRKGDKIKFIVSPYIKNGYVKLW
CCCCCEEEECCEEECCCCCCCEEEEEECCCCEEECCCCCCCCEEEEEEECCCCCCEEEEE
VLDEKPTIIDE
EECCCCCCCCC
>Mature Secondary Structure
MLWRYNPNTKIELRRKYPVTPSDFPLSDIVKTIFESKSIAIFGDYDVDGISSSLILKNAL
CCEEECCCCEEEEEEECCCCCCCCCHHHHHHHHHCCCCEEEEECCCCCCCCHHHHHHHHH
DVLGIQNKIFLPEREEGYGIKPEHVQQIKAEGFDAVITVDNGITAFEAAKAAREAGIKLI
HHHCCCCEEECCCCCCCCCCCHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHCCEEEE
ITDHHEPLAHLPDAYKIFNPKLYKDKYQDYAGAGVAYLIARELLRQRNFSVTPAMIQLAG
EECCCCCHHHCCCCHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHH
LATVADVVPLDGNNWYIARKGLELMRKQPIKGIKEILRTAGRNHENLTGFDIGWIIAPRI
HHHHHHEEECCCCCEEEECCCHHHHHHCCHHHHHHHHHHHCCCCCCCCCEEEEEEEECCC
NATGRLKSPKMGLELLLYGTNAEEVEQLNRKRLELVNQYLSRIEDSENKFLIYAFKNCPR
CCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCC
GITGLIAGRAAEKFQKPVMVSSIDSSGKAVSSARTYGDFDLMEAFKYVSEHVDITFGGHK
CHHHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHCCCCHHHHHHHHHHHHCEEEEECCCC
SAAGVSYSIRDLKRIQNLLNKYTEENPPKEEIRDLDGVLTKVPDLEEVMAFDSFEPFGYK
CCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCCCCHHHHHHHCCCCCCCCC
NPEPAFLLEGTVTDVRADQDWQLVIVNGEFGFFLDNTYRKGDKIKFIVSPYIKNGYVKLW
CCCCCEEEECCEEECCCCCCCEEEEEECCCCEEECCCCCCCCEEEEEEECCCCCCEEEEE
VLDEKPTIIDE
EECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]