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The map label for this gene is AtoC2 [H]

Identifier: 20808401

GI number: 20808401

Start: 1946298

End: 1947947

Strand: Reverse

Name: AtoC2 [H]

Synonym: TTE1998

Alternate gene names: 20808401

Gene position: 1947947-1946298 (Counterclockwise)

Preceding gene: 20808402

Following gene: 20808400

Centisome position: 72.43

GC content: 39.15

Gene sequence:

>1650_bases
ATGCGTGGTGATAAAATGGTGGAAAAGAGGGAAAGGTTGAGACTACCTGTGAGCATCCATTCTCAAATTGCTGCCCACAT
AGTTCAAGAAGTAAGCCGCATAAGCAAAAGGAACGATGTCAGCATATTTTTTAGAAAAATCAATGAGAGTAGGTTGGTTC
CTGCTGGTAGCATGCTTTCCATGGTGACCTTTTTTGCCTCTCGTGGGGAAGAAATAGAAGTGATAATAGAAGGGTTAAAG
GCTGAAAAGGCTTTTGACGAATTTATGCAGTTTTTCAATTCTGAACTGGGCAAGGAAAAAGAAGAAAAAGACACGTATGA
GCTTTTGAGGAATACTTCTATAGCCTATGAGAAAATTTTTAATTCCATCGGGTCAGGACTTGTGGTTACTGACGAAAATG
GAGTTATAACAATATTTAACAAACTGGCAGAAGGCATTACAGGGATAAGCATGGATGAAGCTATAGGCAAGAAAATTCAA
GAGGTCATCCCGGGAATAAAAATTGAGAATGTACTAAAAACGGGGAAAGAGGAAATGAACAAAAAGATTAAGGTTGGCAA
TAATGTTGTGTTTACCAATATAACGCCTATTTATACTGGCAATAAAATAGAAGGGTCTGTAATAGTCTTTTATGATTTTC
CTCAGATAGAGTATCTGGAAAACGAGTTAAAAAGGAACAGGAAGCTGGATAAAGCTTTTGACATAATAATAGGGAATAGT
GGAAAGTTAAGGGATGCCCTCACTATTGCTTCAAAGGCCGCAGAAACGGATTCCACTGTTTTGATAAGAGGAGAGAGTGG
CACGGGAAAGGAGCTGGTAGCACAGGCAATCCACTACGCCAGCAGAAGAAGGGATAAGCCCTTCATAAGGGTGAACTGTG
CCGCTATTCCTGTTACATTGCTGGAAAGCGAGCTTTTCGGCCATGAAAGAGGAGCTTTTACGGGGGCTGTAACGCAGAAG
ATAGGGAAATTTGAACTGGCGGACGGTGGCACCGTATTTTTGGATGAGATTGGGGAAATACCTCCGGAGATTCAGGTAAA
GCTTTTGAGAGTTATACAGGAAAAAGAGTTTGAAAGGGTTGGGGGAATAAAGACTATAAAGGTAGATGTGAGGATAATTG
CAGCCACAAATAAAGACCTTGAAAAGGCAATAAAAGAAGGTACTTTTAGAGAAGATTTGTATTACCGTCTGAATGTTATA
CCAATAATGCTGCCGCCATTGAGGGAGAGACGCGGCGATATACCATTGCTTGTGGAGCATTTTATAGAAAAACTCAATAA
GAAGCTCAATAAAAATATAAAAGGTATTACGAAGAAAGCCATGCAAGCTTTAATTCACTATGACTGGCCTGGAAACATAA
GGGAATTAGAGAACATAATAGAAAGATGTATCACTTTAAGCGAAGGTGAATATATAGACTATGAAGACTTACCTCAATAC
ATACGGAATGAAGATACAATCGTTTCAGAAAACAATGTGATTTATTTGGATGAGCACAGCGAATTACTTACAATGGAAGA
ATATGAATACAAGATAATAAAAGCGGCCTTAGATAGATACAAGAGTTTTAACAAAGCGGCTAAAGTTTTAGGCCTTACTC
ATAAGACAGTCGCTTCCAAGGCCCGGAAATTCGGACTTGTTGATAGATAA

Upstream 100 bases:

>100_bases
AAGCTCCTGTTAAAATTGGAGATGTGATAGTTAAAAACATATTGGGAACGGGGGTTGACGTGATTGCCACTAGAAATCTT
TATAAAAAAGAAGTAAGTTA

Downstream 100 bases:

>100_bases
TACCAACTTACCCTGTCGCTTGATACTTTTTCCCAATATAGCCTGCATGGGCTTTTTTATTTTTGGGGAAAATAAGCAAA
AAAAAACTGGCATGGTTTTT

Product: ATPase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 549; Mature: 549

Protein sequence:

>549_residues
MRGDKMVEKRERLRLPVSIHSQIAAHIVQEVSRISKRNDVSIFFRKINESRLVPAGSMLSMVTFFASRGEEIEVIIEGLK
AEKAFDEFMQFFNSELGKEKEEKDTYELLRNTSIAYEKIFNSIGSGLVVTDENGVITIFNKLAEGITGISMDEAIGKKIQ
EVIPGIKIENVLKTGKEEMNKKIKVGNNVVFTNITPIYTGNKIEGSVIVFYDFPQIEYLENELKRNRKLDKAFDIIIGNS
GKLRDALTIASKAAETDSTVLIRGESGTGKELVAQAIHYASRRRDKPFIRVNCAAIPVTLLESELFGHERGAFTGAVTQK
IGKFELADGGTVFLDEIGEIPPEIQVKLLRVIQEKEFERVGGIKTIKVDVRIIAATNKDLEKAIKEGTFREDLYYRLNVI
PIMLPPLRERRGDIPLLVEHFIEKLNKKLNKNIKGITKKAMQALIHYDWPGNIRELENIIERCITLSEGEYIDYEDLPQY
IRNEDTIVSENNVIYLDEHSELLTMEEYEYKIIKAALDRYKSFNKAAKVLGLTHKTVASKARKFGLVDR

Sequences:

>Translated_549_residues
MRGDKMVEKRERLRLPVSIHSQIAAHIVQEVSRISKRNDVSIFFRKINESRLVPAGSMLSMVTFFASRGEEIEVIIEGLK
AEKAFDEFMQFFNSELGKEKEEKDTYELLRNTSIAYEKIFNSIGSGLVVTDENGVITIFNKLAEGITGISMDEAIGKKIQ
EVIPGIKIENVLKTGKEEMNKKIKVGNNVVFTNITPIYTGNKIEGSVIVFYDFPQIEYLENELKRNRKLDKAFDIIIGNS
GKLRDALTIASKAAETDSTVLIRGESGTGKELVAQAIHYASRRRDKPFIRVNCAAIPVTLLESELFGHERGAFTGAVTQK
IGKFELADGGTVFLDEIGEIPPEIQVKLLRVIQEKEFERVGGIKTIKVDVRIIAATNKDLEKAIKEGTFREDLYYRLNVI
PIMLPPLRERRGDIPLLVEHFIEKLNKKLNKNIKGITKKAMQALIHYDWPGNIRELENIIERCITLSEGEYIDYEDLPQY
IRNEDTIVSENNVIYLDEHSELLTMEEYEYKIIKAALDRYKSFNKAAKVLGLTHKTVASKARKFGLVDR
>Mature_549_residues
MRGDKMVEKRERLRLPVSIHSQIAAHIVQEVSRISKRNDVSIFFRKINESRLVPAGSMLSMVTFFASRGEEIEVIIEGLK
AEKAFDEFMQFFNSELGKEKEEKDTYELLRNTSIAYEKIFNSIGSGLVVTDENGVITIFNKLAEGITGISMDEAIGKKIQ
EVIPGIKIENVLKTGKEEMNKKIKVGNNVVFTNITPIYTGNKIEGSVIVFYDFPQIEYLENELKRNRKLDKAFDIIIGNS
GKLRDALTIASKAAETDSTVLIRGESGTGKELVAQAIHYASRRRDKPFIRVNCAAIPVTLLESELFGHERGAFTGAVTQK
IGKFELADGGTVFLDEIGEIPPEIQVKLLRVIQEKEFERVGGIKTIKVDVRIIAATNKDLEKAIKEGTFREDLYYRLNVI
PIMLPPLRERRGDIPLLVEHFIEKLNKKLNKNIKGITKKAMQALIHYDWPGNIRELENIIERCITLSEGEYIDYEDLPQY
IRNEDTIVSENNVIYLDEHSELLTMEEYEYKIIKAALDRYKSFNKAAKVLGLTHKTVASKARKFGLVDR

Specific function: Member Of The Two-Component Regulatory System Atos/Atoc Involved In The Transcriptional Regulation Of The Ato Genes For Acetoacetate Metabolism. Also An Inhibitor Of Polyamine Biosynthesis. [C]

COG id: COG3829

COG function: function code KT; Transcriptional regulator containing PAS, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1788550, Length=312, Percent_Identity=50.3205128205128, Blast_Score=311, Evalue=7e-86,
Organism=Escherichia coli, GI1790437, Length=306, Percent_Identity=50.3267973856209, Blast_Score=305, Evalue=4e-84,
Organism=Escherichia coli, GI87082117, Length=325, Percent_Identity=47.3846153846154, Blast_Score=303, Evalue=2e-83,
Organism=Escherichia coli, GI1789087, Length=322, Percent_Identity=47.5155279503106, Blast_Score=295, Evalue=6e-81,
Organism=Escherichia coli, GI1790299, Length=349, Percent_Identity=42.1203438395415, Blast_Score=275, Evalue=6e-75,
Organism=Escherichia coli, GI1789233, Length=454, Percent_Identity=36.5638766519824, Blast_Score=266, Evalue=3e-72,
Organism=Escherichia coli, GI1788905, Length=369, Percent_Identity=39.2953929539295, Blast_Score=262, Evalue=5e-71,
Organism=Escherichia coli, GI87081872, Length=232, Percent_Identity=49.5689655172414, Blast_Score=231, Evalue=7e-62,
Organism=Escherichia coli, GI87082152, Length=214, Percent_Identity=50.9345794392523, Blast_Score=230, Evalue=2e-61,
Organism=Escherichia coli, GI1786524, Length=240, Percent_Identity=47.9166666666667, Blast_Score=225, Evalue=6e-60,
Organism=Escherichia coli, GI1787583, Length=431, Percent_Identity=33.4106728538283, Blast_Score=225, Evalue=6e-60,
Organism=Escherichia coli, GI87081858, Length=436, Percent_Identity=27.9816513761468, Blast_Score=161, Evalue=1e-40,
Organism=Escherichia coli, GI1789828, Length=224, Percent_Identity=35.2678571428571, Blast_Score=136, Evalue=4e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR002197
- InterPro:   IPR016040
- InterPro:   IPR001610
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR002078 [H]

Pfam domain/function: PF02954 HTH_8; PF00989 PAS; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 62338; Mature: 62338

Theoretical pI: Translated: 7.70; Mature: 7.70

Prosite motif: PS50112 PAS ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRGDKMVEKRERLRLPVSIHSQIAAHIVQEVSRISKRNDVSIFFRKINESRLVPAGSMLS
CCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHEEHHCCCCCCCCHHHHH
MVTFFASRGEEIEVIIEGLKAEKAFDEFMQFFNSELGKEKEEKDTYELLRNTSIAYEKIF
HHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHH
NSIGSGLVVTDENGVITIFNKLAEGITGISMDEAIGKKIQEVIPGIKIENVLKTGKEEMN
HHHCCCEEEECCCCEEHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCHHHHC
KKIKVGNNVVFTNITPIYTGNKIEGSVIVFYDFPQIEYLENELKRNRKLDKAFDIIIGNS
CCEECCCEEEEEECEEEEECCEECCCEEEEECCCCHHHHHHHHHHCCCHHHHHEEEECCC
GKLRDALTIASKAAETDSTVLIRGESGTGKELVAQAIHYASRRRDKPFIRVNCAAIPVTL
CCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHH
LESELFGHERGAFTGAVTQKIGKFELADGGTVFLDEIGEIPPEIQVKLLRVIQEKEFERV
HHHHHHCCCCCCCHHHHHHHCCCEEECCCCEEEHHHHCCCCHHHHHHHHHHHHHHHHHHH
GGIKTIKVDVRIIAATNKDLEKAIKEGTFREDLYYRLNVIPIMLPPLRERRGDIPLLVEH
CCEEEEEEEEEEEEECCHHHHHHHHCCCCHHHHHEEEEEEEEECCCHHHHCCCCCHHHHH
FIEKLNKKLNKNIKGITKKAMQALIHYDWPGNIRELENIIERCITLSEGEYIDYEDLPQY
HHHHHHHHHCCCHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHH
IRNEDTIVSENNVIYLDEHSELLTMEEYEYKIIKAALDRYKSFNKAAKVLGLTHKTVASK
HCCCCCEEECCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARKFGLVDR
HHHCCCCCC
>Mature Secondary Structure
MRGDKMVEKRERLRLPVSIHSQIAAHIVQEVSRISKRNDVSIFFRKINESRLVPAGSMLS
CCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHEEHHCCCCCCCCHHHHH
MVTFFASRGEEIEVIIEGLKAEKAFDEFMQFFNSELGKEKEEKDTYELLRNTSIAYEKIF
HHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCHHHHHHHH
NSIGSGLVVTDENGVITIFNKLAEGITGISMDEAIGKKIQEVIPGIKIENVLKTGKEEMN
HHHCCCEEEECCCCEEHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCHHHHHHCCHHHHC
KKIKVGNNVVFTNITPIYTGNKIEGSVIVFYDFPQIEYLENELKRNRKLDKAFDIIIGNS
CCEECCCEEEEEECEEEEECCEECCCEEEEECCCCHHHHHHHHHHCCCHHHHHEEEECCC
GKLRDALTIASKAAETDSTVLIRGESGTGKELVAQAIHYASRRRDKPFIRVNCAAIPVTL
CCHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEEEEHHHHHHH
LESELFGHERGAFTGAVTQKIGKFELADGGTVFLDEIGEIPPEIQVKLLRVIQEKEFERV
HHHHHHCCCCCCCHHHHHHHCCCEEECCCCEEEHHHHCCCCHHHHHHHHHHHHHHHHHHH
GGIKTIKVDVRIIAATNKDLEKAIKEGTFREDLYYRLNVIPIMLPPLRERRGDIPLLVEH
CCEEEEEEEEEEEEECCHHHHHHHHCCCCHHHHHEEEEEEEEECCCHHHHCCCCCHHHHH
FIEKLNKKLNKNIKGITKKAMQALIHYDWPGNIRELENIIERCITLSEGEYIDYEDLPQY
HHHHHHHHHCCCHHHHHHHHHHHHEECCCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHH
IRNEDTIVSENNVIYLDEHSELLTMEEYEYKIIKAALDRYKSFNKAAKVLGLTHKTVASK
HCCCCCEEECCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARKFGLVDR
HHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969508; 9384377 [H]