Click here to switch to the map view.

The map label for this gene is BirA [H]

Identifier: 20808369

GI number: 20808369

Start: 1910560

End: 1911540

Strand: Direct

Name: BirA [H]

Synonym: TTE1965

Alternate gene names: 20808369

Gene position: 1910560-1911540 (Clockwise)

Preceding gene: 20808328

Following gene: 20808385

Centisome position: 71.04

GC content: 37.31

Gene sequence:

>981_bases
ATGCTCAGACAAAAACTTGTAGAAATTCTAAAGAAAAACAGAGAAGGCTTCATCTCAGGCCAAAAGCTTTCTGAGATTAT
GAAAGTCTCAAGGACGGCTATCTGGAAGCAAATAAAAGAGCTTGAAAAGCTGGGATACAAAATACAGGCTCATCGCAAAC
TGGGCTATCGCTTGATTTCAGAGCCTGACCTTTTAATTTACGAGGAAATCGCACCCTATCTCAATACCAAATTCATAGGA
AGAAATTACATACATAAAGAAAGCTTAAGCTCTACAAATGACATGGCAAAAGAGCTCGCTTACAAAGTGCCAGATGGAAC
AGTAATTGTAGCAGAAGAACAGACAAAAGGCAGAGGGAGAATGGGCAGAAGTTGGTTCTCTGAAAAAAGCTCCTGTATCC
TTACATCCGTAATATTAAAGCCTCAAATAAGACCTGAAAAGACTGTGGCTTTGACTCAAGTAGCAGCTTTAACAGTGGTA
AAGGCAATAGAAGAGGTTTGCAGCGTAAAAACAAAAATCAAATGGCCAAACGATATAATTCTAAACAGCAAGAAAGTTTG
CGGGATTTTGACAGAAATGTCATCGGAAATAGATGTCGTAAACTACATAGTGATAGGTATAGGAATAAATGTAAACTGTA
CAGAATTCCCAGATGACTTAAAAGAAAAAGCCACCTCTTTGCAGTTGGAATTAGAAAAGCCTGTGGACAGAAAAAAGCTT
TTGGCTTCTCTCTTAAACAATTTGGAAATTTATTACAAAGAATATGAAGAAAAGGGGTTTGAATCATTAAGGCCTCTAAT
ACTTGAAAATTCTATAACAATAGGGAAAGAAGTGAGAGTAATTTACACTGACAGAGAAATAAGAGGGCAGGCCATCGCAA
TAGATAAAGAGGGAAAACTAGTAATAAAGACAGAAAAAGGTGAAGAAATAGCCCTCCTATCAGGGGAAGTCTCTGTAAGA
GGACTTTTGGACTACATTTAA

Upstream 100 bases:

>100_bases
CCGCCCCAAAAGCCCCGCATTTAATTCAATTATATTATTTATTGGAAAGTAAGTAAACCTGGTTTATAATATTTTCTAGA
GGAAAAAATGGGGTGAAAAA

Downstream 100 bases:

>100_bases
ACTTCGCCGTGAAAATATTCATAAACTGCTTTTGCTGACCTCTCATTCATTCCCTTAACTTTTTTAAGTTCCTCTAAAGA
GGCCTTTTTTATCTCCTCAA

Product: biotin-(acetyl-CoA carboxylase) ligase

Products: NA

Alternate protein names: Biotin operon repressor; Biotin--[acetyl-CoA-carboxylase] synthetase; Biotin--protein ligase [H]

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MLRQKLVEILKKNREGFISGQKLSEIMKVSRTAIWKQIKELEKLGYKIQAHRKLGYRLISEPDLLIYEEIAPYLNTKFIG
RNYIHKESLSSTNDMAKELAYKVPDGTVIVAEEQTKGRGRMGRSWFSEKSSCILTSVILKPQIRPEKTVALTQVAALTVV
KAIEEVCSVKTKIKWPNDIILNSKKVCGILTEMSSEIDVVNYIVIGIGINVNCTEFPDDLKEKATSLQLELEKPVDRKKL
LASLLNNLEIYYKEYEEKGFESLRPLILENSITIGKEVRVIYTDREIRGQAIAIDKEGKLVIKTEKGEEIALLSGEVSVR
GLLDYI

Sequences:

>Translated_326_residues
MLRQKLVEILKKNREGFISGQKLSEIMKVSRTAIWKQIKELEKLGYKIQAHRKLGYRLISEPDLLIYEEIAPYLNTKFIG
RNYIHKESLSSTNDMAKELAYKVPDGTVIVAEEQTKGRGRMGRSWFSEKSSCILTSVILKPQIRPEKTVALTQVAALTVV
KAIEEVCSVKTKIKWPNDIILNSKKVCGILTEMSSEIDVVNYIVIGIGINVNCTEFPDDLKEKATSLQLELEKPVDRKKL
LASLLNNLEIYYKEYEEKGFESLRPLILENSITIGKEVRVIYTDREIRGQAIAIDKEGKLVIKTEKGEEIALLSGEVSVR
GLLDYI
>Mature_326_residues
MLRQKLVEILKKNREGFISGQKLSEIMKVSRTAIWKQIKELEKLGYKIQAHRKLGYRLISEPDLLIYEEIAPYLNTKFIG
RNYIHKESLSSTNDMAKELAYKVPDGTVIVAEEQTKGRGRMGRSWFSEKSSCILTSVILKPQIRPEKTVALTQVAALTVV
KAIEEVCSVKTKIKWPNDIILNSKKVCGILTEMSSEIDVVNYIVIGIGINVNCTEFPDDLKEKATSLQLELEKPVDRKKL
LASLLNNLEIYYKEYEEKGFESLRPLILENSITIGKEVRVIYTDREIRGQAIAIDKEGKLVIKTEKGEEIALLSGEVSVR
GLLDYI

Specific function: BirA acts both as a biotin-operon repressor and as the enzyme that synthesizes the corepressor, acetyl-CoA:carbon-dioxide ligase. This protein also activates biotin to form biotinyl-5'- adenylate and transfers the biotin moiety to biotin-accepting protein

COG id: COG0340

COG function: function code H; Biotin-(acetyl-CoA carboxylase) ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the biotin--protein ligase family [H]

Homologues:

Organism=Homo sapiens, GI46255045, Length=251, Percent_Identity=29.8804780876494, Blast_Score=87, Evalue=2e-17,
Organism=Escherichia coli, GI1790408, Length=323, Percent_Identity=29.7213622291022, Blast_Score=124, Evalue=8e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: =6.3.4.15 [H]

Molecular weight: Translated: 37020; Mature: 37020

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: PS00268 CECROPIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLRQKLVEILKKNREGFISGQKLSEIMKVSRTAIWKQIKELEKLGYKIQAHRKLGYRLIS
CCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHCCEECC
EPDLLIYEEIAPYLNTKFIGRNYIHKESLSSTNDMAKELAYKVPDGTVIVAEEQTKGRGR
CCCEEEHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCC
MGRSWFSEKSSCILTSVILKPQIRPEKTVALTQVAALTVVKAIEEVCSVKTKIKWPNDII
CCHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCEE
LNSKKVCGILTEMSSEIDVVNYIVIGIGINVNCTEFPDDLKEKATSLQLELEKPVDRKKL
ECCHHHHHHHHHHCCCCCEEEEEEEEECCEECCCCCCHHHHHHHHEEEEEECCCCCHHHH
LASLLNNLEIYYKEYEEKGFESLRPLILENSITIGKEVRVIYTDREIRGQAIAIDKEGKL
HHHHHHHHHHHHHHHHHCCHHHHCHHHHCCCEECCCEEEEEEECCCCCCCEEEEECCCCE
VIKTEKGEEIALLSGEVSVRGLLDYI
EEEECCCCEEEEEECCCHHHHHHHCC
>Mature Secondary Structure
MLRQKLVEILKKNREGFISGQKLSEIMKVSRTAIWKQIKELEKLGYKIQAHRKLGYRLIS
CCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEHHHHCCEECC
EPDLLIYEEIAPYLNTKFIGRNYIHKESLSSTNDMAKELAYKVPDGTVIVAEEQTKGRGR
CCCEEEHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHHCCCCCEEEEEECCCCCCCC
MGRSWFSEKSSCILTSVILKPQIRPEKTVALTQVAALTVVKAIEEVCSVKTKIKWPNDII
CCHHHHHCCCCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCEE
LNSKKVCGILTEMSSEIDVVNYIVIGIGINVNCTEFPDDLKEKATSLQLELEKPVDRKKL
ECCHHHHHHHHHHCCCCCEEEEEEEEECCEECCCCCCHHHHHHHHEEEEEECCCCCHHHH
LASLLNNLEIYYKEYEEKGFESLRPLILENSITIGKEVRVIYTDREIRGQAIAIDKEGKL
HHHHHHHHHHHHHHHHHCCHHHHCHHHHCCCEECCCEEEEEEECCCCCCCEEEEECCCCE
VIKTEKGEEIALLSGEVSVRGLLDYI
EEEECCCCEEEEEECCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7730294 [H]