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The map label for this gene is pfkA [H]

Identifier: 20808233

GI number: 20808233

Start: 1764656

End: 1765621

Strand: Reverse

Name: pfkA [H]

Synonym: TTE1816

Alternate gene names: 20808233

Gene position: 1765621-1764656 (Counterclockwise)

Preceding gene: 20808234

Following gene: 20808232

Centisome position: 65.65

GC content: 41.1

Gene sequence:

>966_bases
ATGAAGACGATTGGAATACTTACAAGCGGTGGAGATGCTCCTGGCATGAATGCAGCTATAAGGGCTGTGGTGAGGACAGG
AATTTACTACGGCCTTAAGGTAAAGGGTATTATGAGAGGATTTGCAGGTCTTGTAGAAGATGAAGTGATAGATTTGGGGC
TTTCTTCAGTAGGAGATATTATACAAAAAGGTGGGACAATCTTAAGGACTGCTCGTTGCGAGGAGTTCAAGCAAAAAGAG
GTGAGAAAAAAGGCATACGAGACTTTGCAGAAACATGGAATTGAAGGATTGGTTGTAATCGGAGGAGATGGTTCCTTCAG
AGGTGCTCAGCTTCTTAGCGAAGAGTGGAATGTCAATACGATTTGTATACCTGGCACTATTGACAATGACATCCCTTGTA
CAGATTATACTATTGGATTTGATACAGCCTGCAACACTGTGATTGATGCTATAAATAAAATAAGAGATACAGCAACTTCC
CATGAGAGAGCGAATATAATAGAGGTCATGGGTAGAAATTCAGGGTACATCGCCCTTTACGCTGGTGTGGCTGGTGGAGC
GGAGATGATAATTTTGCCTGAAGTGGAATGGAGTATAGATGAATTATGTGATAAAATAACTTATGGAATAAAGAGAGGAA
AACTTCACCACATAATCGTATTGGCAGAGGGAGTAATGAGCGCCCCAGAACTGGCAAAAATGATAAAAGAAAGGCTTCCC
AAGTTAGACTTAAGGTACACCATATTAGGACATATTCAAAGAGGGGGAGCTCCCACTGTGATGGACAGAGTTTTGGCAAG
TCAGATGGGAGCAAGGGCAGTGGAGCTTCTTTTGGAGAACAAGACGAAGAGAGTAATAAGCATAAGAAACAATCAAATTG
TTGACGATGATATAGATGAAGCGCTTTCAATGAAGAAAGAGTTTAACAGAAAGCTTTACGAACTCAGCAAAATCCTCTCT
ATATAA

Upstream 100 bases:

>100_bases
TCATTATTGAATACGGCTTATAATTTTTTTTGCCCGCAAGGGCTTCCTTGACAAAATAGGGACATATGATGTCCAAGTGG
GATAAAAAAGGAGGGACATT

Downstream 100 bases:

>100_bases
AATTTTTTAAGGAGTGAAGTTATGAGGAGAACAAAAATAGTCTGTACAATTGGACCTGCTAGTGAGGACTACAATATACT
GAGAAAGTTAATAGAAAAAG

Product: 6-phosphofructokinase

Products: NA

Alternate protein names: Phosphofructokinase; Phosphohexokinase [H]

Number of amino acids: Translated: 321; Mature: 321

Protein sequence:

>321_residues
MKTIGILTSGGDAPGMNAAIRAVVRTGIYYGLKVKGIMRGFAGLVEDEVIDLGLSSVGDIIQKGGTILRTARCEEFKQKE
VRKKAYETLQKHGIEGLVVIGGDGSFRGAQLLSEEWNVNTICIPGTIDNDIPCTDYTIGFDTACNTVIDAINKIRDTATS
HERANIIEVMGRNSGYIALYAGVAGGAEMIILPEVEWSIDELCDKITYGIKRGKLHHIIVLAEGVMSAPELAKMIKERLP
KLDLRYTILGHIQRGGAPTVMDRVLASQMGARAVELLLENKTKRVISIRNNQIVDDDIDEALSMKKEFNRKLYELSKILS
I

Sequences:

>Translated_321_residues
MKTIGILTSGGDAPGMNAAIRAVVRTGIYYGLKVKGIMRGFAGLVEDEVIDLGLSSVGDIIQKGGTILRTARCEEFKQKE
VRKKAYETLQKHGIEGLVVIGGDGSFRGAQLLSEEWNVNTICIPGTIDNDIPCTDYTIGFDTACNTVIDAINKIRDTATS
HERANIIEVMGRNSGYIALYAGVAGGAEMIILPEVEWSIDELCDKITYGIKRGKLHHIIVLAEGVMSAPELAKMIKERLP
KLDLRYTILGHIQRGGAPTVMDRVLASQMGARAVELLLENKTKRVISIRNNQIVDDDIDEALSMKKEFNRKLYELSKILS
I
>Mature_321_residues
MKTIGILTSGGDAPGMNAAIRAVVRTGIYYGLKVKGIMRGFAGLVEDEVIDLGLSSVGDIIQKGGTILRTARCEEFKQKE
VRKKAYETLQKHGIEGLVVIGGDGSFRGAQLLSEEWNVNTICIPGTIDNDIPCTDYTIGFDTACNTVIDAINKIRDTATS
HERANIIEVMGRNSGYIALYAGVAGGAEMIILPEVEWSIDELCDKITYGIKRGKLHHIIVLAEGVMSAPELAKMIKERLP
KLDLRYTILGHIQRGGAPTVMDRVLASQMGARAVELLLENKTKRVISIRNNQIVDDDIDEALSMKKEFNRKLYELSKILS
I

Specific function: Key control step of glycolysis. [C]

COG id: COG0205

COG function: function code G; 6-phosphofructokinase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphofructokinase family [H]

Homologues:

Organism=Homo sapiens, GI11321601, Length=350, Percent_Identity=40.5714285714286, Blast_Score=235, Evalue=4e-62,
Organism=Homo sapiens, GI48762920, Length=349, Percent_Identity=39.8280802292264, Blast_Score=235, Evalue=5e-62,
Organism=Homo sapiens, GI266453768, Length=351, Percent_Identity=37.3219373219373, Blast_Score=225, Evalue=4e-59,
Organism=Homo sapiens, GI266453748, Length=351, Percent_Identity=37.3219373219373, Blast_Score=225, Evalue=4e-59,
Organism=Homo sapiens, GI4505749, Length=351, Percent_Identity=37.3219373219373, Blast_Score=225, Evalue=4e-59,
Organism=Homo sapiens, GI266453619, Length=351, Percent_Identity=37.3219373219373, Blast_Score=225, Evalue=5e-59,
Organism=Escherichia coli, GI1790350, Length=320, Percent_Identity=49.375, Blast_Score=300, Evalue=1e-82,
Organism=Caenorhabditis elegans, GI25147584, Length=332, Percent_Identity=40.9638554216867, Blast_Score=223, Evalue=1e-58,
Organism=Caenorhabditis elegans, GI17558788, Length=314, Percent_Identity=36.9426751592357, Blast_Score=196, Evalue=2e-50,
Organism=Caenorhabditis elegans, GI72003601, Length=309, Percent_Identity=28.1553398058252, Blast_Score=136, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6323861, Length=345, Percent_Identity=37.3913043478261, Blast_Score=214, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6321679, Length=342, Percent_Identity=37.4269005847953, Blast_Score=205, Evalue=8e-54,
Organism=Drosophila melanogaster, GI24652337, Length=331, Percent_Identity=42.2960725075529, Blast_Score=238, Evalue=4e-63,
Organism=Drosophila melanogaster, GI17647809, Length=331, Percent_Identity=42.2960725075529, Blast_Score=238, Evalue=4e-63,
Organism=Drosophila melanogaster, GI28573326, Length=331, Percent_Identity=41.9939577039275, Blast_Score=236, Evalue=2e-62,

Paralogues:

None

Copy number: 3981 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 950 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012003
- InterPro:   IPR012828
- InterPro:   IPR022953
- InterPro:   IPR015912
- InterPro:   IPR000023 [H]

Pfam domain/function: PF00365 PFK [H]

EC number: =2.7.1.11 [H]

Molecular weight: Translated: 35176; Mature: 35176

Theoretical pI: Translated: 7.02; Mature: 7.02

Prosite motif: PS00433 PHOSPHOFRUCTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKTIGILTSGGDAPGMNAAIRAVVRTGIYYGLKVKGIMRGFAGLVEDEVIDLGLSSVGDI
CCEEEEEECCCCCCCHHHHHHHHHHHHHHHCEEHHHHHHHHHHHHHHHHHHHCHHHHHHH
IQKGGTILRTARCEEFKQKEVRKKAYETLQKHGIEGLVVIGGDGSFRGAQLLSEEWNVNT
HHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHCCCCEE
ICIPGTIDNDIPCTDYTIGFDTACNTVIDAINKIRDTATSHERANIIEVMGRNSGYIALY
EEECCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEEE
AGVAGGAEMIILPEVEWSIDELCDKITYGIKRGKLHHIIVLAEGVMSAPELAKMIKERLP
EECCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHCC
KLDLRYTILGHIQRGGAPTVMDRVLASQMGARAVELLLENKTKRVISIRNNQIVDDDIDE
CCCEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHH
ALSMKKEFNRKLYELSKILSI
HHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKTIGILTSGGDAPGMNAAIRAVVRTGIYYGLKVKGIMRGFAGLVEDEVIDLGLSSVGDI
CCEEEEEECCCCCCCHHHHHHHHHHHHHHHCEEHHHHHHHHHHHHHHHHHHHCHHHHHHH
IQKGGTILRTARCEEFKQKEVRKKAYETLQKHGIEGLVVIGGDGSFRGAQLLSEEWNVNT
HHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHCCCCEE
ICIPGTIDNDIPCTDYTIGFDTACNTVIDAINKIRDTATSHERANIIEVMGRNSGYIALY
EEECCCCCCCCCCCCEEECHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCEEEEE
AGVAGGAEMIILPEVEWSIDELCDKITYGIKRGKLHHIIVLAEGVMSAPELAKMIKERLP
EECCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCEEEEEEEECCCCCCHHHHHHHHHHCC
KLDLRYTILGHIQRGGAPTVMDRVLASQMGARAVELLLENKTKRVISIRNNQIVDDDIDE
CCCEEHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHH
ALSMKKEFNRKLYELSKILSI
HHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA