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The map label for this gene is PykF [H]

Identifier: 20808232

GI number: 20808232

Start: 1762883

End: 1764634

Strand: Reverse

Name: PykF [H]

Synonym: TTE1815

Alternate gene names: 20808232

Gene position: 1764634-1762883 (Counterclockwise)

Preceding gene: 20808233

Following gene: 20808231

Centisome position: 65.61

GC content: 41.84

Gene sequence:

>1752_bases
ATGAGGAGAACAAAAATAGTCTGTACAATTGGACCTGCTAGTGAGGACTACAATATACTGAGAAAGTTAATAGAAAAAGG
AATGAATGTAGCAAGGCTTAATTTTTCCCATGGAGATTTTGAAGAGCACGGAGCGAGGATTGACAACATAAAAAAGATAA
GGGAAGAATTAGGTCTTCCTGTAGCTATTTTGCTGGACACAAAAGGACCCGAGATTAGAACGGGAAAGTTTAAAAACGGC
GGAGTAGAGTTAAAGGAAGGCCAGACTTTTACATTAACTACAAGGGATGTAATAGGAGATGAAACGGTAGTCAGTGTTTC
CTACAAAGGTTTGCCTCAAGATGTTTCAAGAGGTACCCAAATTTTGATTGATGACGGATTGATCTCACTAAGGGTTGTGG
ATGTCAAAGGGGAAGACATAATCTGTGTAGTTGAAAATTCGGGATTTCTTGGAGACCACAAAGGAGTAAATGTTCCTGGA
GTGAAATTGAATCTCCCGGCTTTGACACAAAAAGACATAGAAGATATAGAGTTTGGCATAAAGAAAGGTATTGACATGAT
AGCTGCTTCTTTTGTCAGGAAAGCGGCAGATGTGCTTGCTATAAGGAGGCTTCTTGAGGACAATAAAGCAGACCACATTC
AGATAATTGCTAAAATAGAAAACCGCGAAGGAGTAGAAAATATAGACGAAATAATAAGGGTATCTGACGGGATAATGGTG
GCCAGAGGAGATTTGGGCGTTGAGATACCTCTGGAGGAAATACCGATAGTGCAGAAGATGATAATAAAGAAGTGCAATGA
AGCTGGAAAGCCAGTTATTACTGCAACCCAAATGCTGGATTCCATGATGAGAAATCCGCGTCCTACTCGTGCTGAAGTTA
CGGATGTTGCTAATGCTATTTTGGATGGAACGGATGCCATAATGCTGTCAGGTGAGACGGCCCAGGGCAAATATCCTGTC
GAAGCTTTTGAGACAATGGCCAGGATTGCGGAAAAGACAGAGGTTTACGTCCAGTACAGAGATATAGTAGGTGTAGGTAC
AGAGAGAAATGTTTCCATTACTAATGCGATAAGCCATGCTACCTGCACGACTGCGAGAGATATAGGCGCTTCAGCTATCA
TAACCTGTACAAAATCAGGTTATACGGCTAGGATGGTTTCAAGGTATAGGCCTTCCTCTCCTATCATAGCTACTACTCCT
AGCGAGCAGGTGGCAAGAAAGCTTTCCATCGTGTGGGGAGTATATCCTTTAGTGACAAAAGAAGTGTCAACCACAGACGA
AATGATAGATGTTGCAATAGAAAGTGCGTTAAAAGCTGGGCTTATAAGGAATGGAGATATTGTGGTGATTTCTGCGGGAA
TACCTGTTGCCATGACTGGCACCACAAACATGCTCAAAGTTCACATAGTTGGAGATGTGATTTTAAGAGGTATTGGTATA
GGTTCAAAATCTACTACTGGCACTGTGTGCATAATTCATGACATTGGCAAGGACAGAGACAAGTTTAGAGAAGGAGATAT
AATTGTTACAACCAAGACAGAGAGAGATTTTATGCCTCTTATTGAAAAAGCTTCTGCCATAATAACAGAAGAAGGCGGGC
TCACCTCCCACGCTGCTATTGTTGGGCTTAACTTGGGGATCCCTGTGATTGTAGGGTGTGAAGGAGCTACTTCAAAACTT
CAGGATGGGATGACAGTCACTGTTGACACCACGAGAGGACTGGTGTATAAAGGAATTGTAAACATAAAATGA

Upstream 100 bases:

>100_bases
TGATATAGATGAAGCGCTTTCAATGAAGAAAGAGTTTAACAGAAAGCTTTACGAACTCAGCAAAATCCTCTCTATATAAA
ATTTTTTAAGGAGTGAAGTT

Downstream 100 bases:

>100_bases
TTGGCTCTTTCTACTTTTTAAGGAGTGATTTGAATGCATATTTGGGATGCGGAGATAAGAGTCCGCTATGGTGAAACTGA
TAAAATGGGAGTAGTATACT

Product: pyruvate kinase

Products: NA

Alternate protein names: PK [H]

Number of amino acids: Translated: 583; Mature: 583

Protein sequence:

>583_residues
MRRTKIVCTIGPASEDYNILRKLIEKGMNVARLNFSHGDFEEHGARIDNIKKIREELGLPVAILLDTKGPEIRTGKFKNG
GVELKEGQTFTLTTRDVIGDETVVSVSYKGLPQDVSRGTQILIDDGLISLRVVDVKGEDIICVVENSGFLGDHKGVNVPG
VKLNLPALTQKDIEDIEFGIKKGIDMIAASFVRKAADVLAIRRLLEDNKADHIQIIAKIENREGVENIDEIIRVSDGIMV
ARGDLGVEIPLEEIPIVQKMIIKKCNEAGKPVITATQMLDSMMRNPRPTRAEVTDVANAILDGTDAIMLSGETAQGKYPV
EAFETMARIAEKTEVYVQYRDIVGVGTERNVSITNAISHATCTTARDIGASAIITCTKSGYTARMVSRYRPSSPIIATTP
SEQVARKLSIVWGVYPLVTKEVSTTDEMIDVAIESALKAGLIRNGDIVVISAGIPVAMTGTTNMLKVHIVGDVILRGIGI
GSKSTTGTVCIIHDIGKDRDKFREGDIIVTTKTERDFMPLIEKASAIITEEGGLTSHAAIVGLNLGIPVIVGCEGATSKL
QDGMTVTVDTTRGLVYKGIVNIK

Sequences:

>Translated_583_residues
MRRTKIVCTIGPASEDYNILRKLIEKGMNVARLNFSHGDFEEHGARIDNIKKIREELGLPVAILLDTKGPEIRTGKFKNG
GVELKEGQTFTLTTRDVIGDETVVSVSYKGLPQDVSRGTQILIDDGLISLRVVDVKGEDIICVVENSGFLGDHKGVNVPG
VKLNLPALTQKDIEDIEFGIKKGIDMIAASFVRKAADVLAIRRLLEDNKADHIQIIAKIENREGVENIDEIIRVSDGIMV
ARGDLGVEIPLEEIPIVQKMIIKKCNEAGKPVITATQMLDSMMRNPRPTRAEVTDVANAILDGTDAIMLSGETAQGKYPV
EAFETMARIAEKTEVYVQYRDIVGVGTERNVSITNAISHATCTTARDIGASAIITCTKSGYTARMVSRYRPSSPIIATTP
SEQVARKLSIVWGVYPLVTKEVSTTDEMIDVAIESALKAGLIRNGDIVVISAGIPVAMTGTTNMLKVHIVGDVILRGIGI
GSKSTTGTVCIIHDIGKDRDKFREGDIIVTTKTERDFMPLIEKASAIITEEGGLTSHAAIVGLNLGIPVIVGCEGATSKL
QDGMTVTVDTTRGLVYKGIVNIK
>Mature_583_residues
MRRTKIVCTIGPASEDYNILRKLIEKGMNVARLNFSHGDFEEHGARIDNIKKIREELGLPVAILLDTKGPEIRTGKFKNG
GVELKEGQTFTLTTRDVIGDETVVSVSYKGLPQDVSRGTQILIDDGLISLRVVDVKGEDIICVVENSGFLGDHKGVNVPG
VKLNLPALTQKDIEDIEFGIKKGIDMIAASFVRKAADVLAIRRLLEDNKADHIQIIAKIENREGVENIDEIIRVSDGIMV
ARGDLGVEIPLEEIPIVQKMIIKKCNEAGKPVITATQMLDSMMRNPRPTRAEVTDVANAILDGTDAIMLSGETAQGKYPV
EAFETMARIAEKTEVYVQYRDIVGVGTERNVSITNAISHATCTTARDIGASAIITCTKSGYTARMVSRYRPSSPIIATTP
SEQVARKLSIVWGVYPLVTKEVSTTDEMIDVAIESALKAGLIRNGDIVVISAGIPVAMTGTTNMLKVHIVGDVILRGIGI
GSKSTTGTVCIIHDIGKDRDKFREGDIIVTTKTERDFMPLIEKASAIITEEGGLTSHAAIVGLNLGIPVIVGCEGATSKL
QDGMTVTVDTTRGLVYKGIVNIK

Specific function: Glycolysis; final step. [C]

COG id: COG0469

COG function: function code G; Pyruvate kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the PEP- utilizing enzyme family [H]

Homologues:

Organism=Homo sapiens, GI33286422, Length=487, Percent_Identity=44.7638603696099, Blast_Score=409, Evalue=1e-114,
Organism=Homo sapiens, GI33286420, Length=487, Percent_Identity=44.7638603696099, Blast_Score=409, Evalue=1e-114,
Organism=Homo sapiens, GI33286418, Length=488, Percent_Identity=45.6967213114754, Blast_Score=407, Evalue=1e-113,
Organism=Homo sapiens, GI10835121, Length=489, Percent_Identity=45.398773006135, Blast_Score=397, Evalue=1e-110,
Organism=Homo sapiens, GI32967597, Length=489, Percent_Identity=45.398773006135, Blast_Score=396, Evalue=1e-110,
Organism=Homo sapiens, GI310128732, Length=288, Percent_Identity=50.3472222222222, Blast_Score=273, Evalue=4e-73,
Organism=Homo sapiens, GI310128730, Length=288, Percent_Identity=50.3472222222222, Blast_Score=273, Evalue=4e-73,
Organism=Homo sapiens, GI310128736, Length=236, Percent_Identity=47.4576271186441, Blast_Score=209, Evalue=8e-54,
Organism=Homo sapiens, GI310128734, Length=236, Percent_Identity=47.4576271186441, Blast_Score=209, Evalue=8e-54,
Organism=Homo sapiens, GI310128738, Length=215, Percent_Identity=46.9767441860465, Blast_Score=184, Evalue=2e-46,
Organism=Escherichia coli, GI1787965, Length=472, Percent_Identity=51.6949152542373, Blast_Score=481, Evalue=1e-137,
Organism=Escherichia coli, GI1788160, Length=479, Percent_Identity=41.1273486430063, Blast_Score=313, Evalue=2e-86,
Organism=Escherichia coli, GI1787994, Length=107, Percent_Identity=43.9252336448598, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17544584, Length=482, Percent_Identity=46.0580912863071, Blast_Score=408, Evalue=1e-114,
Organism=Caenorhabditis elegans, GI17506829, Length=484, Percent_Identity=45.2479338842975, Blast_Score=368, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI71984413, Length=484, Percent_Identity=45.2479338842975, Blast_Score=368, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI71984406, Length=484, Percent_Identity=45.0413223140496, Blast_Score=367, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17506831, Length=484, Percent_Identity=45.0413223140496, Blast_Score=367, Evalue=1e-102,
Organism=Saccharomyces cerevisiae, GI6319279, Length=486, Percent_Identity=45.2674897119342, Blast_Score=379, Evalue=1e-106,
Organism=Saccharomyces cerevisiae, GI6324923, Length=486, Percent_Identity=41.9753086419753, Blast_Score=355, Evalue=2e-98,
Organism=Drosophila melanogaster, GI24648964, Length=490, Percent_Identity=45.9183673469388, Blast_Score=405, Evalue=1e-113,
Organism=Drosophila melanogaster, GI28571814, Length=490, Percent_Identity=45.9183673469388, Blast_Score=404, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24648966, Length=417, Percent_Identity=47.9616306954436, Blast_Score=369, Evalue=1e-102,
Organism=Drosophila melanogaster, GI24581235, Length=470, Percent_Identity=38.0851063829787, Blast_Score=293, Evalue=2e-79,
Organism=Drosophila melanogaster, GI24646914, Length=278, Percent_Identity=46.0431654676259, Blast_Score=230, Evalue=2e-60,

Paralogues:

None

Copy number: 500 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 124 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR001697
- InterPro:   IPR015813
- InterPro:   IPR011037
- InterPro:   IPR015794
- InterPro:   IPR018209
- InterPro:   IPR015793
- InterPro:   IPR015795
- InterPro:   IPR015806 [H]

Pfam domain/function: PF00391 PEP-utilizers; PF00224 PK; PF02887 PK_C [H]

EC number: =2.7.1.40 [H]

Molecular weight: Translated: 63010; Mature: 63010

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: PS00110 PYRUVATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRRTKIVCTIGPASEDYNILRKLIEKGMNVARLNFSHGDFEEHGARIDNIKKIREELGLP
CCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHCCCHHHHHHHHHHHCCC
VAILLDTKGPEIRTGKFKNGGVELKEGQTFTLTTRDVIGDETVVSVSYKGLPQDVSRGTQ
EEEEEECCCCCEECCCCCCCCEEEECCCEEEEEEHHHCCCCEEEEEEECCCCHHHCCCCE
ILIDDGLISLRVVDVKGEDIICVVENSGFLGDHKGVNVPGVKLNLPALTQKDIEDIEFGI
EEEECCEEEEEEEEECCCEEEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHH
KKGIDMIAASFVRKAADVLAIRRLLEDNKADHIQIIAKIENREGVENIDEIIRVSDGIMV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCEEE
ARGDLGVEIPLEEIPIVQKMIIKKCNEAGKPVITATQMLDSMMRNPRPTRAEVTDVANAI
EECCCCEECCHHHCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCCCHHHHHHHHHH
LDGTDAIMLSGETAQGKYPVEAFETMARIAEKTEVYVQYRDIVGVGTERNVSITNAISHA
HCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHEEEEEEEEEEECCCCCCEEHHHHHHHH
TCTTARDIGASAIITCTKSGYTARMVSRYRPSSPIIATTPSEQVARKLSIVWGVYPLVTK
HHHHHHHCCCEEEEEEECCCCHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHC
EVSTTDEMIDVAIESALKAGLIRNGDIVVISAGIPVAMTGTTNMLKVHIVGDVILRGIGI
CCCCHHHHHHHHHHHHHHHCCEECCCEEEEECCCCEEEECCCCEEEEEEEEHHHHHHCCC
GSKSTTGTVCIIHDIGKDRDKFREGDIIVTTKTERDFMPLIEKASAIITEEGGLTSHAAI
CCCCCCCEEEEEECCCCCHHHHCCCCEEEEECCCCHHHHHHHHHHEEEECCCCCCCCEEE
VGLNLGIPVIVGCEGATSKLQDGMTVTVDTTRGLVYKGIVNIK
EEEECCCCEEEECCCCHHHHHCCCEEEEECCCCEEEEEEEECC
>Mature Secondary Structure
MRRTKIVCTIGPASEDYNILRKLIEKGMNVARLNFSHGDFEEHGARIDNIKKIREELGLP
CCCEEEEEEECCCCCCHHHHHHHHHCCCCEEEEECCCCCHHHHCCCHHHHHHHHHHHCCC
VAILLDTKGPEIRTGKFKNGGVELKEGQTFTLTTRDVIGDETVVSVSYKGLPQDVSRGTQ
EEEEEECCCCCEECCCCCCCCEEEECCCEEEEEEHHHCCCCEEEEEEECCCCHHHCCCCE
ILIDDGLISLRVVDVKGEDIICVVENSGFLGDHKGVNVPGVKLNLPALTQKDIEDIEFGI
EEEECCEEEEEEEEECCCEEEEEEECCCCCCCCCCCCCCCEEEECCCCCCCCHHHHHHHH
KKGIDMIAASFVRKAADVLAIRRLLEDNKADHIQIIAKIENREGVENIDEIIRVSDGIMV
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCHHHHHHHHHHCCCEEE
ARGDLGVEIPLEEIPIVQKMIIKKCNEAGKPVITATQMLDSMMRNPRPTRAEVTDVANAI
EECCCCEECCHHHCCHHHHHHHHHHHHCCCCEEHHHHHHHHHHHCCCCCCHHHHHHHHHH
LDGTDAIMLSGETAQGKYPVEAFETMARIAEKTEVYVQYRDIVGVGTERNVSITNAISHA
HCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHEEEEEEEEEEECCCCCCEEHHHHHHHH
TCTTARDIGASAIITCTKSGYTARMVSRYRPSSPIIATTPSEQVARKLSIVWGVYPLVTK
HHHHHHHCCCEEEEEEECCCCHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHC
EVSTTDEMIDVAIESALKAGLIRNGDIVVISAGIPVAMTGTTNMLKVHIVGDVILRGIGI
CCCCHHHHHHHHHHHHHHHCCEECCCEEEEECCCCEEEECCCCEEEEEEEEHHHHHHCCC
GSKSTTGTVCIIHDIGKDRDKFREGDIIVTTKTERDFMPLIEKASAIITEEGGLTSHAAI
CCCCCCCEEEEEECCCCCHHHHCCCCEEEEECCCCHHHHHHHHHHEEEECCCCCCCCEEE
VGLNLGIPVIVGCEGATSKLQDGMTVTVDTTRGLVYKGIVNIK
EEEECCCCEEEECCCCHHHHHCCCEEEEECCCCEEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8436141; 1447787 [H]