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The map label for this gene is MetF [H]

Identifier: 20808221

GI number: 20808221

Start: 1749301

End: 1750206

Strand: Reverse

Name: MetF [H]

Synonym: TTE1804

Alternate gene names: 20808221

Gene position: 1750206-1749301 (Counterclockwise)

Preceding gene: 20808223

Following gene: 20808220

Centisome position: 65.08

GC content: 38.85

Gene sequence:

>906_bases
ATGTTGAAAAATGAAAAGCTGTGTAATAAACTTAAAGAAAAGAAATTTGTAATAACTGTGGAAATTTCTCCCCCCAAAGG
GATAGATGTAACTAAAACTATCGAGGAAGCTCGAAAACTTAAAGGTGTGGCAGATGCTCTCAATATAACTGATTCACCGA
TGGCAAATATGAGGATGAGCCCCATAGCCTTGGCGCATATACTTCAGGACCACCTTAACATTGAAACTATTTTTCATCTA
ACCTGCAGAGATAGAAATCTCATAGGGCTTCAATCAGAGCTTTTAGGAGCTTACGCCCTGGGGGTTAGAAATATACTAGC
TTTAACCGGTGATAATCCTTCTTTGGGAGATACACCCCAAGCGAGGGGAGTCTACGATGTAGATTCTGTAGGCCTTTTGA
AGATTATAAAAGCTTTGAATTCAGGTTTTGATTACAATGGGAATAAGCTCGATTATCCTACTTCTTTCTGTGCAGGAACT
ACTGCCAATCCGAATGACTTGAGGGATGAGGCCATAGAAAAGCTGCATGTGAAAGTAGAAGCAGGAGCTGAATTTATACA
GACCCAACCGGTGTATGATGAGGAGATTCTTTTTAAGTTTTTGGACAAAGTAAAAGATCTGAAAGTACCTGTCCTTGTGG
GAATACTGCCTTTAAAAAGCTATAAAATGGCTTTAAGATTGATGGAGAAAGTGCCAGGGATTGAAATACCAGAAAAAGTT
ATAAAAAGGCTCCAACATGGCGGAAAAGAGGAAGGAATAAACTTATCCGCCGAGTTTATAAAGAACATAAAAAGTGAAGT
TGCGGGGATTCACATAATGCCCATGGGAAGTGTAGACTTTGTTTTTGATATAATCTCTAAAGTGGAAGGAATAGCTATAA
GTGCTAAAAAGGCTGAGACGAAGTAA

Upstream 100 bases:

>100_bases
TGCCAATTCCTGCAGCGGTTTCGCTGAAAGATGAGAGATTCTTGTAGTCTCTTCTTTTAGCGAAGGGACTTTTTTATTTT
TAAAAAAGGAGGGGCATTAA

Downstream 100 bases:

>100_bases
TGGGGGGATGAAAGTGGTAGATATTTTTAAAGAATGTGAAAATAGGGTTGTCGTTTTTGACGGTGCTATGGGGACACAGC
TTCAGGAAAGGGGACTTAAA

Product: 5,10-methylenetetrahydrofolate reductase

Products: NA

Alternate protein names: Homocysteine S-methyltransferase; S-methylmethionine:homocysteine methyltransferase; 5,10-methylenetetrahydrofolate reductase [H]

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MLKNEKLCNKLKEKKFVITVEISPPKGIDVTKTIEEARKLKGVADALNITDSPMANMRMSPIALAHILQDHLNIETIFHL
TCRDRNLIGLQSELLGAYALGVRNILALTGDNPSLGDTPQARGVYDVDSVGLLKIIKALNSGFDYNGNKLDYPTSFCAGT
TANPNDLRDEAIEKLHVKVEAGAEFIQTQPVYDEEILFKFLDKVKDLKVPVLVGILPLKSYKMALRLMEKVPGIEIPEKV
IKRLQHGGKEEGINLSAEFIKNIKSEVAGIHIMPMGSVDFVFDIISKVEGIAISAKKAETK

Sequences:

>Translated_301_residues
MLKNEKLCNKLKEKKFVITVEISPPKGIDVTKTIEEARKLKGVADALNITDSPMANMRMSPIALAHILQDHLNIETIFHL
TCRDRNLIGLQSELLGAYALGVRNILALTGDNPSLGDTPQARGVYDVDSVGLLKIIKALNSGFDYNGNKLDYPTSFCAGT
TANPNDLRDEAIEKLHVKVEAGAEFIQTQPVYDEEILFKFLDKVKDLKVPVLVGILPLKSYKMALRLMEKVPGIEIPEKV
IKRLQHGGKEEGINLSAEFIKNIKSEVAGIHIMPMGSVDFVFDIISKVEGIAISAKKAETK
>Mature_301_residues
MLKNEKLCNKLKEKKFVITVEISPPKGIDVTKTIEEARKLKGVADALNITDSPMANMRMSPIALAHILQDHLNIETIFHL
TCRDRNLIGLQSELLGAYALGVRNILALTGDNPSLGDTPQARGVYDVDSVGLLKIIKALNSGFDYNGNKLDYPTSFCAGT
TANPNDLRDEAIEKLHVKVEAGAEFIQTQPVYDEEILFKFLDKVKDLKVPVLVGILPLKSYKMALRLMEKVPGIEIPEKV
IKRLQHGGKEEGINLSAEFIKNIKSEVAGIHIMPMGSVDFVFDIISKVEGIAISAKKAETK

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Hcy-binding domain [H]

Homologues:

Organism=Caenorhabditis elegans, GI25147441, Length=298, Percent_Identity=25.8389261744966, Blast_Score=77, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI25147436, Length=298, Percent_Identity=25.8389261744966, Blast_Score=77, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6325234, Length=269, Percent_Identity=28.6245353159851, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR003726 [H]

Pfam domain/function: PF02219 MTHFR; PF02574 S-methyl_trans [H]

EC number: =2.1.1.10; =1.5.1.20 [H]

Molecular weight: Translated: 33175; Mature: 33175

Theoretical pI: Translated: 7.59; Mature: 7.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKNEKLCNKLKEKKFVITVEISPPKGIDVTKTIEEARKLKGVADALNITDSPMANMRMS
CCCCHHHHHHHHHCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCC
PIALAHILQDHLNIETIFHLTCRDRNLIGLQSELLGAYALGVRNILALTGDNPSLGDTPQ
HHHHHHHHHHHCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCCC
ARGVYDVDSVGLLKIIKALNSGFDYNGNKLDYPTSFCAGTTANPNDLRDEAIEKLHVKVE
CCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHH
AGAEFIQTQPVYDEEILFKFLDKVKDLKVPVLVGILPLKSYKMALRLMEKVPGIEIPEKV
HCCHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
IKRLQHGGKEEGINLSAEFIKNIKSEVAGIHIMPMGSVDFVFDIISKVEGIAISAKKAET
HHHHHCCCCCCCCCCHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCEEEECCCCCC
K
C
>Mature Secondary Structure
MLKNEKLCNKLKEKKFVITVEISPPKGIDVTKTIEEARKLKGVADALNITDSPMANMRMS
CCCCHHHHHHHHHCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHCCCCC
PIALAHILQDHLNIETIFHLTCRDRNLIGLQSELLGAYALGVRNILALTGDNPSLGDTPQ
HHHHHHHHHHHCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHEEEEECCCCCCCCCCC
ARGVYDVDSVGLLKIIKALNSGFDYNGNKLDYPTSFCAGTTANPNDLRDEAIEKLHVKVE
CCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHHH
AGAEFIQTQPVYDEEILFKFLDKVKDLKVPVLVGILPLKSYKMALRLMEKVPGIEIPEKV
HCCHHHHCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHH
IKRLQHGGKEEGINLSAEFIKNIKSEVAGIHIMPMGSVDFVFDIISKVEGIAISAKKAET
HHHHHCCCCCCCCCCHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHHHCCEEEECCCCCC
K
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9353932; 9384377 [H]