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The map label for this gene is RpoD4 [H]

Identifier: 20808174

GI number: 20808174

Start: 1701569

End: 1702687

Strand: Reverse

Name: RpoD4 [H]

Synonym: TTE1755

Alternate gene names: 20808174

Gene position: 1702687-1701569 (Counterclockwise)

Preceding gene: 20808175

Following gene: 20808173

Centisome position: 63.31

GC content: 41.2

Gene sequence:

>1119_bases
ATGGGCAGAAAGAGAGAAACCCATAGGGAGGGGAAATCTATGAGTAACAATGAAGAATTAGCAAGAGAAGCCATTAATGA
ACTTATAAATAAAGGGAAAAAAACCGGAATGCTCACTTACAATGAAATAATGGACTCCCTTGAGGATATAGATTTAAATC
CTGACCAAATCGAAAAAGTATATGACACTTTTGAAGATATGGGAATAGAAATAGTAGGAGATGAACCCCGACAGGAAGAA
ATATCAGAAGAGGACCTCAATTTGGACGACCTCTCTCTTCCTGAGGGAGTCAGCATAGATGACCCTGTGAGGATGTACCT
CAAAGAAATCGGCAAGATACCTCTACTCACTCCAGAAGAAGAAATTGAATTGGCAAAAAGAATAGAGCAGGGAGATGAAG
AAGCGAAAAAGCGCCTGATAGAAGCTAATTTGAGGTTGGTGGTAAGCATTGCAAAAAGGTACGTAGGGCGTGGCATGCTC
TTTTTAGACTTGATACAAGAGGGTAATCTCGGGCTTTTGAAGGCCGTAGAAAAATTTGACTACAGAAAAGGGTATAAATT
CAGCACCTATGCTACATGGTGGATCAGACAGGCTATAACTAGAGCTATAGCTGATCAAGCTAGGACGATAAGAATTCCTG
TGCACATGGTAGAAACTATAAACAAATTGATAAGGGTGCAAAGGCAATTGCTCCAGGAATTAGGCCGTGAACCTACCCCT
GAAGAATTGGCAAAAGAAATGGGAATGCCTGAAGAAAAAGTCAGAGAAATTCTCAAAATAGCTCAAGAACCAGTTTCTCT
TGAGACTCCTATAGGTGAAGAGGAAGACAGTCACTTGGGAGATTTCATACCTGATGAAGATGCTCCGGCTCCTGCAGAAG
CTGCAGCTTACACCATGCTGAAAGAGCAACTCATGGATGTTTTAGATACGCTGACTCCTAGGGAGGAGAAGGTATTGCGG
TTAAGGTTTGGACTTGACGATGGACGAGCTAGAACTCTTGAAGAAGTTGGCAAAGAATTTAACGTCACAAGAGAGAGAAT
AAGGCAGATTGAAGCAAAAGCTTTGAGAAAATTGAGGCATCCTTCAAGGTCCAAAAAATTAAAAGATTTTCTCGATTGA

Upstream 100 bases:

>100_bases
AAAGAGATATTAAATGCTCATCTTCTGGGGGATGTAGAAAAAGAGAGAGAGCTTCTTATTAAACTTCAAAATTGTGAAAA
GGAGATGCTTAAGATAAAAG

Downstream 100 bases:

>100_bases
TGATGGCTTCTTGACAATCTCGCAAAAATCATTATAATTAATATTAGCGTATTCCTCAGTAGCTCAAGGGTAGAGCAGCC
GGCTGTTAACCGGCAGGTTG

Product: RNA polymerase sigma factor RpoD

Products: NA

Alternate protein names: Sigma-43; Sigma-A [H]

Number of amino acids: Translated: 372; Mature: 371

Protein sequence:

>372_residues
MGRKRETHREGKSMSNNEELAREAINELINKGKKTGMLTYNEIMDSLEDIDLNPDQIEKVYDTFEDMGIEIVGDEPRQEE
ISEEDLNLDDLSLPEGVSIDDPVRMYLKEIGKIPLLTPEEEIELAKRIEQGDEEAKKRLIEANLRLVVSIAKRYVGRGML
FLDLIQEGNLGLLKAVEKFDYRKGYKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVQRQLLQELGREPTP
EELAKEMGMPEEKVREILKIAQEPVSLETPIGEEEDSHLGDFIPDEDAPAPAEAAAYTMLKEQLMDVLDTLTPREEKVLR
LRFGLDDGRARTLEEVGKEFNVTRERIRQIEAKALRKLRHPSRSKKLKDFLD

Sequences:

>Translated_372_residues
MGRKRETHREGKSMSNNEELAREAINELINKGKKTGMLTYNEIMDSLEDIDLNPDQIEKVYDTFEDMGIEIVGDEPRQEE
ISEEDLNLDDLSLPEGVSIDDPVRMYLKEIGKIPLLTPEEEIELAKRIEQGDEEAKKRLIEANLRLVVSIAKRYVGRGML
FLDLIQEGNLGLLKAVEKFDYRKGYKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVQRQLLQELGREPTP
EELAKEMGMPEEKVREILKIAQEPVSLETPIGEEEDSHLGDFIPDEDAPAPAEAAAYTMLKEQLMDVLDTLTPREEKVLR
LRFGLDDGRARTLEEVGKEFNVTRERIRQIEAKALRKLRHPSRSKKLKDFLD
>Mature_371_residues
GRKRETHREGKSMSNNEELAREAINELINKGKKTGMLTYNEIMDSLEDIDLNPDQIEKVYDTFEDMGIEIVGDEPRQEEI
SEEDLNLDDLSLPEGVSIDDPVRMYLKEIGKIPLLTPEEEIELAKRIEQGDEEAKKRLIEANLRLVVSIAKRYVGRGMLF
LDLIQEGNLGLLKAVEKFDYRKGYKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVQRQLLQELGREPTPE
ELAKEMGMPEEKVREILKIAQEPVSLETPIGEEEDSHLGDFIPDEDAPAPAEAAAYTMLKEQLMDVLDTLTPREEKVLRL
RFGLDDGRARTLEEVGKEFNVTRERIRQIEAKALRKLRHPSRSKKLKDFLD

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This is the primary sigma factor of this bacterium [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789448, Length=260, Percent_Identity=67.3076923076923, Blast_Score=360, Evalue=1e-101,
Organism=Escherichia coli, GI1789098, Length=332, Percent_Identity=41.566265060241, Blast_Score=253, Evalue=9e-69,
Organism=Escherichia coli, GI1789871, Length=272, Percent_Identity=30.5147058823529, Blast_Score=119, Evalue=3e-28,
Organism=Escherichia coli, GI1788231, Length=199, Percent_Identity=28.643216080402, Blast_Score=72, Evalue=6e-14,

Paralogues:

None

Copy number: 700 (log & stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR007127
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012760
- InterPro:   IPR011991 [H]

Pfam domain/function: PF03979 Sigma70_r1_1; PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 42894; Mature: 42762

Theoretical pI: Translated: 4.66; Mature: 4.66

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2 ; PS00867 CPSASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGRKRETHREGKSMSNNEELAREAINELINKGKKTGMLTYNEIMDSLEDIDLNPDQIEKV
CCCCCHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHCCCCHHHHHHH
YDTFEDMGIEIVGDEPRQEEISEEDLNLDDLSLPEGVSIDDPVRMYLKEIGKIPLLTPEE
HHHHHHCCEEEECCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHH
EIELAKRIEQGDEEAKKRLIEANLRLVVSIAKRYVGRGMLFLDLIQEGNLGLLKAVEKFD
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHH
YRKGYKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVQRQLLQELGREPTP
HHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
EELAKEMGMPEEKVREILKIAQEPVSLETPIGEEEDSHLGDFIPDEDAPAPAEAAAYTML
HHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
KEQLMDVLDTLTPREEKVLRLRFGLDDGRARTLEEVGKEFNVTRERIRQIEAKALRKLRH
HHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PSRSKKLKDFLD
CHHHHHHHHHCC
>Mature Secondary Structure 
GRKRETHREGKSMSNNEELAREAINELINKGKKTGMLTYNEIMDSLEDIDLNPDQIEKV
CCCCHHHHHCCCCCCHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHHHHCCCCHHHHHHH
YDTFEDMGIEIVGDEPRQEEISEEDLNLDDLSLPEGVSIDDPVRMYLKEIGKIPLLTPEE
HHHHHHCCEEEECCCCCHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCHH
EIELAKRIEQGDEEAKKRLIEANLRLVVSIAKRYVGRGMLFLDLIQEGNLGLLKAVEKFD
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHH
YRKGYKFSTYATWWIRQAITRAIADQARTIRIPVHMVETINKLIRVQRQLLQELGREPTP
HHCCCCCHHHHHHHHHHHHHHHHHHCCCEEEHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
EELAKEMGMPEEKVREILKIAQEPVSLETPIGEEEDSHLGDFIPDEDAPAPAEAAAYTML
HHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHH
KEQLMDVLDTLTPREEKVLRLRFGLDDGRARTLEEVGKEFNVTRERIRQIEAKALRKLRH
HHHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
PSRSKKLKDFLD
CHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11058132 [H]