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The map label for this gene is dnaG [H]

Identifier: 20808175

GI number: 20808175

Start: 1702662

End: 1704464

Strand: Reverse

Name: dnaG [H]

Synonym: TTE1756

Alternate gene names: 20808175

Gene position: 1704464-1702662 (Counterclockwise)

Preceding gene: 20808176

Following gene: 20808174

Centisome position: 63.38

GC content: 35.5

Gene sequence:

>1803_bases
ATGGCTTATACGAGAGAGTCCATTGAAAAGGTGATAGAGGCCAATGACATTGTAGATGTGATTTCTGACTATGTAGAGCT
CAAAAGAGCTGGAAAGGAATTTCGGGGACTCTGCCCTTTTCACAGGGAGAAGACGCCATCTTTTATGGTAAGCCCTACTA
AACAGGTATACCATTGTTTTGGTTGCAATGCCAGTGGCAATGTAGTGACCTTTATAATGGATATTGAAAATTTAAGCTTT
AAAGAGGCTATTGAATTTTTGGCTGAAAGAGCAGGGATTCCTCTAGAAGAAGTAGATTTAAGTGAAGAAGAGGCCAAGAG
GAAGAAGATAATTGAGGAAATATACAGAGTAAATAAAATAGCTGCTTTGTTTTTTCATCGGAAACTCTTTTCAGAAGAAG
GAGTCAAAGCTTTAGAATACTTAAAGAGTAGAGGACTTAGCAGCACTACAATAAAAAAATTTGGCATAGGGTATGCTCCC
TCAAATGATGACCTCCTTAATTTCTTAAAAGGAAAAGGATATAAAGAAAATTTTTTAAGCAAAGCAGGACTTGTGGTTTA
TAAAAGTGGCAAATACTATGACAGGTTTAAAGATAGGGTCATGTTTCCTATCATCGATGTAAGGGGCAATGTGGTAGGAT
TTGGGGGACGAGCCCTGGATGATTCTTTGCCTAAATATCTTAATACACCTGAGACTGAAATCTTTAAAAAAGGCAAGACT
CTTTTTGCTATAAACTTTGCGAAAAAAACCCAAGATGACAAGTTTATAATAGTGGAAGGATATATGGATGCAATTTCTCT
GCATCAGGCAGGTATTGACTGTGCTGTTGCGTCTTTGGGGACCGCTTTGACTGAAGATCAGGCAAGGCTTATAAAAAGAT
ACAAGAAAAATGTCGTGATTGCTTATGATGCTGATGAAGCTGGGGTTAATGCTACGTTGAGGGGACTTGACATTTTAGAA
AAATTGAATATAAATATAAAAGTGCTGAGCATACCTGAAGGGAAAGACCCTGATGAATTTATTAAAAAAGAGGGAAGCGA
GGCCTTTAGAAGGCTTGTTGAAAATGCTGAAAGTTTGATAGAGTTTAAGTCGAAAATTTTCAAAAAAGGTTTTGACTTTG
AAAAAGTTGAAGATAGAATAATATATGTTAAAAAAATTGCAAGTGAAATAGCAAAAATTTCTGACGAAGTGAAAAGAGAA
GTTTATATTTCAGCTGCTGCTAAAACTGCTCAAATACCTGAAAATGCTGTTAGAACAGAGGTTGACCGATTTGTCAAGAG
GGAAACCGAAAAAAAACAAAAATTTATGTATAGGACTGGCAATATAAGGCATAATATATACAGTAGCAGTAAAATATCAC
CAGAAAAGTACTTAATTGCATTGCTTTTACACGATAATAATTTGTATCACAAGATAAAAGGTGTAATTAAACCGGATATG
TTGGAGGACTCCAAGTTAAAACCTATCTTTGAGGAAATAGTATCGAGTTTAGAGGGAGGAAAACAGGTTCAAATAAAAGA
TATAGTATACCTTATTCAGGAGGAAGATAACTTAATTTCAGATTTTAACGATATAGTAAAAGCCCTTTTTGAAGCGGAGG
ACCTTTCGCAAATGGTGGATGACATCCTGCAGAAGATTTTGCTAAATAATCTTGCAAGAAAGAGAGAAGAGATAAGAAAA
GAGATATTAAATGCTCATCTTCTGGGGGATGTAGAAAAAGAGAGAGAGCTTCTTATTAAACTTCAAAATTGTGAAAAGGA
GATGCTTAAGATAAAAGATGGGCAGAAAGAGAGAAACCCATAG

Upstream 100 bases:

>100_bases
ATAAAAGAAGGATTTTTTAATTTTTTGTCGAATATATATTGGACGTCATAAAAAGAGGAATTTAACAATTTCTGTCGAAT
AGAATAATTAGGTGATATTG

Downstream 100 bases:

>100_bases
GGAGGGGAAATCTATGAGTAACAATGAAGAATTAGCAAGAGAAGCCATTAATGAACTTATAAATAAAGGGAAAAAAACCG
GAATGCTCACTTACAATGAA

Product: DNA primase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 600; Mature: 599

Protein sequence:

>600_residues
MAYTRESIEKVIEANDIVDVISDYVELKRAGKEFRGLCPFHREKTPSFMVSPTKQVYHCFGCNASGNVVTFIMDIENLSF
KEAIEFLAERAGIPLEEVDLSEEEAKRKKIIEEIYRVNKIAALFFHRKLFSEEGVKALEYLKSRGLSSTTIKKFGIGYAP
SNDDLLNFLKGKGYKENFLSKAGLVVYKSGKYYDRFKDRVMFPIIDVRGNVVGFGGRALDDSLPKYLNTPETEIFKKGKT
LFAINFAKKTQDDKFIIVEGYMDAISLHQAGIDCAVASLGTALTEDQARLIKRYKKNVVIAYDADEAGVNATLRGLDILE
KLNINIKVLSIPEGKDPDEFIKKEGSEAFRRLVENAESLIEFKSKIFKKGFDFEKVEDRIIYVKKIASEIAKISDEVKRE
VYISAAAKTAQIPENAVRTEVDRFVKRETEKKQKFMYRTGNIRHNIYSSSKISPEKYLIALLLHDNNLYHKIKGVIKPDM
LEDSKLKPIFEEIVSSLEGGKQVQIKDIVYLIQEEDNLISDFNDIVKALFEAEDLSQMVDDILQKILLNNLARKREEIRK
EILNAHLLGDVEKERELLIKLQNCEKEMLKIKDGQKERNP

Sequences:

>Translated_600_residues
MAYTRESIEKVIEANDIVDVISDYVELKRAGKEFRGLCPFHREKTPSFMVSPTKQVYHCFGCNASGNVVTFIMDIENLSF
KEAIEFLAERAGIPLEEVDLSEEEAKRKKIIEEIYRVNKIAALFFHRKLFSEEGVKALEYLKSRGLSSTTIKKFGIGYAP
SNDDLLNFLKGKGYKENFLSKAGLVVYKSGKYYDRFKDRVMFPIIDVRGNVVGFGGRALDDSLPKYLNTPETEIFKKGKT
LFAINFAKKTQDDKFIIVEGYMDAISLHQAGIDCAVASLGTALTEDQARLIKRYKKNVVIAYDADEAGVNATLRGLDILE
KLNINIKVLSIPEGKDPDEFIKKEGSEAFRRLVENAESLIEFKSKIFKKGFDFEKVEDRIIYVKKIASEIAKISDEVKRE
VYISAAAKTAQIPENAVRTEVDRFVKRETEKKQKFMYRTGNIRHNIYSSSKISPEKYLIALLLHDNNLYHKIKGVIKPDM
LEDSKLKPIFEEIVSSLEGGKQVQIKDIVYLIQEEDNLISDFNDIVKALFEAEDLSQMVDDILQKILLNNLARKREEIRK
EILNAHLLGDVEKERELLIKLQNCEKEMLKIKDGQKERNP
>Mature_599_residues
AYTRESIEKVIEANDIVDVISDYVELKRAGKEFRGLCPFHREKTPSFMVSPTKQVYHCFGCNASGNVVTFIMDIENLSFK
EAIEFLAERAGIPLEEVDLSEEEAKRKKIIEEIYRVNKIAALFFHRKLFSEEGVKALEYLKSRGLSSTTIKKFGIGYAPS
NDDLLNFLKGKGYKENFLSKAGLVVYKSGKYYDRFKDRVMFPIIDVRGNVVGFGGRALDDSLPKYLNTPETEIFKKGKTL
FAINFAKKTQDDKFIIVEGYMDAISLHQAGIDCAVASLGTALTEDQARLIKRYKKNVVIAYDADEAGVNATLRGLDILEK
LNINIKVLSIPEGKDPDEFIKKEGSEAFRRLVENAESLIEFKSKIFKKGFDFEKVEDRIIYVKKIASEIAKISDEVKREV
YISAAAKTAQIPENAVRTEVDRFVKRETEKKQKFMYRTGNIRHNIYSSSKISPEKYLIALLLHDNNLYHKIKGVIKPDML
EDSKLKPIFEEIVSSLEGGKQVQIKDIVYLIQEEDNLISDFNDIVKALFEAEDLSQMVDDILQKILLNNLARKREEIRKE
ILNAHLLGDVEKERELLIKLQNCEKEMLKIKDGQKERNP

Specific function: DNA primase is the polymerase that synthesizes small RNA primers for the Okazaki fragments on both template strands at replication forks during chromosomal DNA synthesis [H]

COG id: COG0358

COG function: function code L; DNA primase (bacterial type)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Escherichia coli, GI1789447, Length=405, Percent_Identity=38.0246913580247, Blast_Score=286, Evalue=4e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013264
- InterPro:   IPR019475
- InterPro:   IPR006295
- InterPro:   IPR006171
- InterPro:   IPR002694 [H]

Pfam domain/function: PF10410 DnaB_bind; PF01751 Toprim; PF08275 Toprim_N; PF01807 zf-CHC2 [H]

EC number: 2.7.7.-

Molecular weight: Translated: 68615; Mature: 68484

Theoretical pI: Translated: 7.88; Mature: 7.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYTRESIEKVIEANDIVDVISDYVELKRAGKEFRGLCPFHREKTPSFMVSPTKQVYHCF
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCCEEECCHHHHHHHC
GCNASGNVVTFIMDIENLSFKEAIEFLAERAGIPLEEVDLSEEEAKRKKIIEEIYRVNKI
CCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHH
AALFFHRKLFSEEGVKALEYLKSRGLSSTTIKKFGIGYAPSNDDLLNFLKGKGYKENFLS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHH
KAGLVVYKSGKYYDRFKDRVMFPIIDVRGNVVGFGGRALDDSLPKYLNTPETEIFKKGKT
HCCEEEEECCCHHHHHHHCEEEEEEECCCCEEECCCCHHHHHHHHHCCCCHHHHHHCCCE
LFAINFAKKTQDDKFIIVEGYMDAISLHQAGIDCAVASLGTALTEDQARLIKRYKKNVVI
EEEEEECCCCCCCCEEEEECHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
AYDADEAGVNATLRGLDILEKLNINIKVLSIPEGKDPDEFIKKEGSEAFRRLVENAESLI
EECCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHCCHHHHHHHHHHHHHHHH
EFKSKIFKKGFDFEKVEDRIIYVKKIASEIAKISDEVKREVYISAAAKTAQIPENAVRTE
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
VDRFVKRETEKKQKFMYRTGNIRHNIYSSSKISPEKYLIALLLHDNNLYHKIKGVIKPDM
HHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHEEEEEEEECCCHHHHHHCCCCCCC
LEDSKLKPIFEEIVSSLEGGKQVQIKDIVYLIQEEDNLISDFNDIVKALFEAEDLSQMVD
CCCCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
DILQKILLNNLARKREEIRKEILNAHLLGDVEKERELLIKLQNCEKEMLKIKDGQKERNP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
AYTRESIEKVIEANDIVDVISDYVELKRAGKEFRGLCPFHREKTPSFMVSPTKQVYHCF
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCCCCCCCCEEECCHHHHHHHC
GCNASGNVVTFIMDIENLSFKEAIEFLAERAGIPLEEVDLSEEEAKRKKIIEEIYRVNKI
CCCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCHHHCCCCHHHHHHHHHHHHHHHHHHH
AALFFHRKLFSEEGVKALEYLKSRGLSSTTIKKFGIGYAPSNDDLLNFLKGKGYKENFLS
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCCCHHHHHHHHCCCCHHHHHH
KAGLVVYKSGKYYDRFKDRVMFPIIDVRGNVVGFGGRALDDSLPKYLNTPETEIFKKGKT
HCCEEEEECCCHHHHHHHCEEEEEEECCCCEEECCCCHHHHHHHHHCCCCHHHHHHCCCE
LFAINFAKKTQDDKFIIVEGYMDAISLHQAGIDCAVASLGTALTEDQARLIKRYKKNVVI
EEEEEECCCCCCCCEEEEECHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHCCEEE
AYDADEAGVNATLRGLDILEKLNINIKVLSIPEGKDPDEFIKKEGSEAFRRLVENAESLI
EECCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHCCHHHHHHHHHHHHHHHH
EFKSKIFKKGFDFEKVEDRIIYVKKIASEIAKISDEVKREVYISAAAKTAQIPENAVRTE
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHH
VDRFVKRETEKKQKFMYRTGNIRHNIYSSSKISPEKYLIALLLHDNNLYHKIKGVIKPDM
HHHHHHHHHHHHHHHHHHCCCEEECCCCCCCCCHHHEEEEEEEECCCHHHHHHCCCCCCC
LEDSKLKPIFEEIVSSLEGGKQVQIKDIVYLIQEEDNLISDFNDIVKALFEAEDLSQMVD
CCCCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
DILQKILLNNLARKREEIRKEILNAHLLGDVEKERELLIKLQNCEKEMLKIKDGQKERNP
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups; Nucleotidyltransferases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7961408; 11466286 [H]