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The map label for this gene is SpoU [H]

Identifier: 20808116

GI number: 20808116

Start: 1642236

End: 1643036

Strand: Reverse

Name: SpoU [H]

Synonym: TTE1690

Alternate gene names: 20808116

Gene position: 1643036-1642236 (Counterclockwise)

Preceding gene: 20808117

Following gene: 20808115

Centisome position: 61.09

GC content: 37.33

Gene sequence:

>801_bases
ATGCTCATAACGAGTGAGGACAATAGCTTTATAAAGAAGATTAAAAAGCTAAAAGATAAAAAAGGGCGATATGAAGAAAA
GCTCTTTTTTGTAGAAGGATTAAACAGCGTAAAAGAAGCCTTGGATAGTGACTTTAAAGTGGAGTATGTGGTAGTAGGGG
AAGATGTGGAATTTGAGTTTAAAGGAGATTTTAAGCTAATACGCATTGCAAATAAGCTGTTTAAAAAAATCAGTGATACG
GTGACTCCTCAAAAAATAATGGCGATTGTGAGGATGCCAGTGTACCGCGAAGAAGATTTTATAAAAGAAGAGGGCGTGTA
CGTTATAGCAGATAATGTACAGGACCCGGGAAATCTTGGGACAATCATAAGAACGGCTGATGCTTTTGGGGCTGATGCTG
TTTTTACAATAAATAACTCTGTCGATATATATAACCCAAAAGTTCTCAGAGCCTCAATGGGGTCAATCTTTCACATTCCT
GTCATCGCTACAGCAAATGAAGTGCTTTACAAACTTAAAGGAAAAGGTGTCAAAGTTTTTGCCACGCACCTAAAAGGGGA
AAAATTTGTGCATGAAGTTGAGATAGACAAAGGTACAGCTTTTATTCTGGGAAATGAAGCGAGGGGAGTGAGCTCTTTAG
ATTTTGTAGACGGCTTTATAAAAATTCCTATGACAGGGAGAGCAGAATCACTGAATGTTTCCATTGCGGCAGCAATATTT
TTGTACGAATCACAAAGGCAGAGGTTGATAAAAAATAAGCAAAATGGTATAATCTTAAAAAAATACAGCCAAATGCGATG
A

Upstream 100 bases:

>100_bases
AAAGGCTTTTGCAGAGTTAGTGAACATAGCTAAACAGCAATTAAACGCATAAGGCCAGTTTTAGATGGCCTTTTGCTTTA
ATAAAAAAGAGTGGTGCAAT

Downstream 100 bases:

>100_bases
AGGAGTAAAGTACATTATCGAAGGCCACAGGGAGATGGCGCCGTAGACTGAGAGCGCCATTGGCAGGTAGATAATGGAAG
TTCCCTCTGGAGCAGCGTGC

Product: rRNA methylase

Products: S-adenosyl-L-homocysteine; tRNATyr Containing 2'-O-methylguanosine [C]

Alternate protein names: NA

Number of amino acids: Translated: 266; Mature: 266

Protein sequence:

>266_residues
MLITSEDNSFIKKIKKLKDKKGRYEEKLFFVEGLNSVKEALDSDFKVEYVVVGEDVEFEFKGDFKLIRIANKLFKKISDT
VTPQKIMAIVRMPVYREEDFIKEEGVYVIADNVQDPGNLGTIIRTADAFGADAVFTINNSVDIYNPKVLRASMGSIFHIP
VIATANEVLYKLKGKGVKVFATHLKGEKFVHEVEIDKGTAFILGNEARGVSSLDFVDGFIKIPMTGRAESLNVSIAAAIF
LYESQRQRLIKNKQNGIILKKYSQMR

Sequences:

>Translated_266_residues
MLITSEDNSFIKKIKKLKDKKGRYEEKLFFVEGLNSVKEALDSDFKVEYVVVGEDVEFEFKGDFKLIRIANKLFKKISDT
VTPQKIMAIVRMPVYREEDFIKEEGVYVIADNVQDPGNLGTIIRTADAFGADAVFTINNSVDIYNPKVLRASMGSIFHIP
VIATANEVLYKLKGKGVKVFATHLKGEKFVHEVEIDKGTAFILGNEARGVSSLDFVDGFIKIPMTGRAESLNVSIAAAIF
LYESQRQRLIKNKQNGIILKKYSQMR
>Mature_266_residues
MLITSEDNSFIKKIKKLKDKKGRYEEKLFFVEGLNSVKEALDSDFKVEYVVVGEDVEFEFKGDFKLIRIANKLFKKISDT
VTPQKIMAIVRMPVYREEDFIKEEGVYVIADNVQDPGNLGTIIRTADAFGADAVFTINNSVDIYNPKVLRASMGSIFHIP
VIATANEVLYKLKGKGVKVFATHLKGEKFVHEVEIDKGTAFILGNEARGVSSLDFVDGFIKIPMTGRAESLNVSIAAAIF
LYESQRQRLIKNKQNGIILKKYSQMR

Specific function: Specifically Methylates Guanosine-18 In Various tRNAs. [C]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Homo sapiens, GI8922534, Length=241, Percent_Identity=29.4605809128631, Blast_Score=89, Evalue=4e-18,
Organism=Escherichia coli, GI1790083, Length=188, Percent_Identity=30.8510638297872, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI1790623, Length=195, Percent_Identity=29.7435897435897, Blast_Score=83, Evalue=2e-17,
Organism=Drosophila melanogaster, GI24666840, Length=285, Percent_Identity=25.2631578947368, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.34 [C]

Molecular weight: Translated: 29987; Mature: 29987

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLITSEDNSFIKKIKKLKDKKGRYEEKLFFVEGLNSVKEALDSDFKVEYVVVGEDVEFEF
CEEECCCCHHHHHHHHHHHCCCCHHHHEEEEHHHHHHHHHHCCCCEEEEEEECCCEEEEE
KGDFKLIRIANKLFKKISDTVTPQKIMAIVRMPVYREEDFIKEEGVYVIADNVQDPGNLG
CCCEEHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHCCEEEEECCCCCCCCCC
TIIRTADAFGADAVFTINNSVDIYNPKVLRASMGSIFHIPVIATANEVLYKLKGKGVKVF
CEEEECCCCCCCEEEEECCCEEECCCEEEEECCCCEEEEEEEECHHHHHHHHCCCCEEEE
ATHLKGEKFVHEVEIDKGTAFILGNEARGVSSLDFVDGFIKIPMTGRAESLNVSIAAAIF
EEECCCCCEEEEEEECCCEEEEEECCCCCCCCHHHCCCEEEECCCCCCCCCCEEEEEEEE
LYESQRQRLIKNKQNGIILKKYSQMR
EEHHHHHHHHHCCCCCEEEECCCCCC
>Mature Secondary Structure
MLITSEDNSFIKKIKKLKDKKGRYEEKLFFVEGLNSVKEALDSDFKVEYVVVGEDVEFEF
CEEECCCCHHHHHHHHHHHCCCCHHHHEEEEHHHHHHHHHHCCCCEEEEEEECCCEEEEE
KGDFKLIRIANKLFKKISDTVTPQKIMAIVRMPVYREEDFIKEEGVYVIADNVQDPGNLG
CCCEEHHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCHHHHCCEEEEECCCCCCCCCC
TIIRTADAFGADAVFTINNSVDIYNPKVLRASMGSIFHIPVIATANEVLYKLKGKGVKVF
CEEEECCCCCCCEEEEECCCEEECCCEEEEECCCCEEEEEEEECHHHHHHHHCCCCEEEE
ATHLKGEKFVHEVEIDKGTAFILGNEARGVSSLDFVDGFIKIPMTGRAESLNVSIAAAIF
EEECCCCCEEEEEEECCCEEEEEECCCCCCCCHHHCCCEEEECCCCCCCCCCEEEEEEEE
LYESQRQRLIKNKQNGIILKKYSQMR
EEHHHHHHHHHCCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: S-Adenosyl-L-methionine; tRNATyr [C]

Specific reaction: S-Adenosyl-L-methionine + tRNATyr = S-adenosyl-L-homocysteine + tRNATyr Containing 2'-O-methylguanosine [C]

General reaction: Methyl group transfer [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]