Click here to switch to the map view.

The map label for this gene is ThiI

Identifier: 20808089

GI number: 20808089

Start: 1612424

End: 1613566

Strand: Reverse

Name: ThiI

Synonym: TTE1662

Alternate gene names: 20808089

Gene position: 1613566-1612424 (Counterclockwise)

Preceding gene: 20808090

Following gene: 20808088

Centisome position: 60.0

GC content: 40.86

Gene sequence:

>1143_bases
ATGAAAGACGTACTTCTCATAAAATACGGAGAATTGGCATTGAAAGGAGAAAACAGGTCTTTTTTTGAAAATACACTTGT
AAAAAATATAAAATACGCTCTAAGAGATTTTGATGGCGTAAAAGTCGAGAAAACCCATGGGAGAATTTATGTGGAGTGCG
AAAAAGATGTTGAAGAGGCAATAGAAAGGTTAAAAAAAGTCTTTGGAATAGTCGGGATAACGAGGGCAAAAGAGGCTGAC
CTTGACCTTGAGGAAATTTTTAAAGCTGCTGTAGATCTCATGAAAAGTCACCAGGGCAAGACTTTCAAGGTAGAAACCAA
AAGGCCCAATAAGGCCTTTCCTTATAATAGCATGGAGGTAAGCAGAAGAGTAGGAGCAGCCGTTTTGAAGAATGTGAAAA
ACATGAAAGTGGATGTACACAATCCTGATGTGCTCCTTAATGTGGAGATAAGGGAAAAAGCCTTTTTATATGCAGGGGTT
ATTGAAGGGGCAGGAGGTCTTCCTCTTGGCACGAATGGAAAAGCGACTGTACTTCTTTCGGGAGGAATTGACAGCCCTGT
AGCGGCATGGATGATGATGAAAAGAGGTGTGGAAGTAGAGGCTGTTTACTTTCACAGCCCTCCGTACACGTCAGATAGGG
CGAAGGAAAAAGTTGTGGACCTGTGTAAAGTGCTTTCTCAATATGGAAGGGGAATGCGACTGCATGTAGTCCACTTCACT
GACTTGCAGCTTGAAATTTACGAAAAATGCCCTGCTAGGTTAACGACAATAATAATGAGAAGAATGATGATGAAAATAGC
GGAAAAAATCGCTCAGCAGAATGGCTCTCTAGCCCTTATAACGGGAGAGAGCCTTGGACAGGTGGCAAGCCAGACCATTG
AAAGCCTGTATGTAACCAATTCTTCTGTTTCACTTCCTGTCTTTAGACCTTTGATTGGGATGGATAAGAGGGAAATAATT
GATATAGCTCAGAAAATTGGCACCTACGAAATCTCCATAAGGCCTTATGAGGACTGCTGTACTATATTCGTTCCAAAACA
TCCTGCTACAAAGCCCAAGCTGGAGAAGGTTTTAGAAGCAGAAGAGAAGATGGATTACCAAAAATTTATAGACAATTTTG
AAGAAGAAGTAATAGAAATTTGA

Upstream 100 bases:

>100_bases
TCTCTGGGGATTTTTAATACTGAGGAAGACATAGATTACACAGTGGATATTTTGAAGAAAAAAGTTGACTTTTTAAGAAG
ATTTAAACGGAGGTAAAATA

Downstream 100 bases:

>100_bases
GTCAGGTTGCACCTGACTCAAATTTATTTTTTAAAAATTAAACTTTTAGCTAGATAAAATTAAAAAATTTAAAATTGATA
TTGACATTTTTGAAGGAAGT

Product: thiamine biosynthesis protein ThiI

Products: NA

Alternate protein names: Sulfur carrier protein ThiS sulfurtransferase; Thiamine biosynthesis protein thiI; tRNA 4-thiouridine synthase

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MKDVLLIKYGELALKGENRSFFENTLVKNIKYALRDFDGVKVEKTHGRIYVECEKDVEEAIERLKKVFGIVGITRAKEAD
LDLEEIFKAAVDLMKSHQGKTFKVETKRPNKAFPYNSMEVSRRVGAAVLKNVKNMKVDVHNPDVLLNVEIREKAFLYAGV
IEGAGGLPLGTNGKATVLLSGGIDSPVAAWMMMKRGVEVEAVYFHSPPYTSDRAKEKVVDLCKVLSQYGRGMRLHVVHFT
DLQLEIYEKCPARLTTIIMRRMMMKIAEKIAQQNGSLALITGESLGQVASQTIESLYVTNSSVSLPVFRPLIGMDKREII
DIAQKIGTYEISIRPYEDCCTIFVPKHPATKPKLEKVLEAEEKMDYQKFIDNFEEEVIEI

Sequences:

>Translated_380_residues
MKDVLLIKYGELALKGENRSFFENTLVKNIKYALRDFDGVKVEKTHGRIYVECEKDVEEAIERLKKVFGIVGITRAKEAD
LDLEEIFKAAVDLMKSHQGKTFKVETKRPNKAFPYNSMEVSRRVGAAVLKNVKNMKVDVHNPDVLLNVEIREKAFLYAGV
IEGAGGLPLGTNGKATVLLSGGIDSPVAAWMMMKRGVEVEAVYFHSPPYTSDRAKEKVVDLCKVLSQYGRGMRLHVVHFT
DLQLEIYEKCPARLTTIIMRRMMMKIAEKIAQQNGSLALITGESLGQVASQTIESLYVTNSSVSLPVFRPLIGMDKREII
DIAQKIGTYEISIRPYEDCCTIFVPKHPATKPKLEKVLEAEEKMDYQKFIDNFEEEVIEI
>Mature_380_residues
MKDVLLIKYGELALKGENRSFFENTLVKNIKYALRDFDGVKVEKTHGRIYVECEKDVEEAIERLKKVFGIVGITRAKEAD
LDLEEIFKAAVDLMKSHQGKTFKVETKRPNKAFPYNSMEVSRRVGAAVLKNVKNMKVDVHNPDVLLNVEIREKAFLYAGV
IEGAGGLPLGTNGKATVLLSGGIDSPVAAWMMMKRGVEVEAVYFHSPPYTSDRAKEKVVDLCKVLSQYGRGMRLHVVHFT
DLQLEIYEKCPARLTTIIMRRMMMKIAEKIAQQNGSLALITGESLGQVASQTIESLYVTNSSVSLPVFRPLIGMDKREII
DIAQKIGTYEISIRPYEDCCTIFVPKHPATKPKLEKVLEAEEKMDYQKFIDNFEEEVIEI

Specific function: Catalyzes the ATP-dependent transfer of a sulfur to tRNA to produce 4-thiouridine in position 8 of tRNAs, which functions as a near-UV photosensor. Also catalyzes the transfer of sulfur to the sulfur carrier protein ThiS, forming ThiS-thiocarboxylate. Thi

COG id: COG0301

COG function: function code H; Thiamine biosynthesis ATP pyrophosphatase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 THUMP domain

Homologues:

Organism=Escherichia coli, GI1786625, Length=367, Percent_Identity=31.6076294277929, Blast_Score=166, Evalue=3e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THII_THETN (Q8R9F0)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_623260.1
- ProteinModelPortal:   Q8R9F0
- SMR:   Q8R9F0
- GeneID:   996217
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE1662
- NMPDR:   fig|273068.3.peg.1644
- HOGENOM:   HBG646072
- OMA:   ESIGQVA
- ProtClustDB:   PRK01565
- BioCyc:   TTEN273068:TTE1662-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_00021
- InterPro:   IPR014729
- InterPro:   IPR003720
- InterPro:   IPR020536
- InterPro:   IPR004114
- Gene3D:   G3DSA:3.40.50.620
- SMART:   SM00981
- TIGRFAMs:   TIGR00342

Pfam domain/function: PF02568 ThiI; PF02926 THUMP

EC number: =2.8.1.4

Molecular weight: Translated: 42900; Mature: 42900

Theoretical pI: Translated: 8.18; Mature: 8.18

Prosite motif: PS51165 THUMP

Important sites: BINDING 260-260 BINDING 282-282 BINDING 291-291

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKDVLLIKYGELALKGENRSFFENTLVKNIKYALRDFDGVKVEKTHGRIYVECEKDVEEA
CCCEEEEEECCEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEECHHHHHH
IERLKKVFGIVGITRAKEADLDLEEIFKAAVDLMKSHQGKTFKVETKRPNKAFPYNSMEV
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHH
SRRVGAAVLKNVKNMKVDVHNPDVLLNVEIREKAFLYAGVIEGAGGLPLGTNGKATVLLS
HHHHHHHHHHHHHHCEEEECCCCEEEEEEEHHHHEEEEEHCCCCCCCCCCCCCCEEEEEE
GGIDSPVAAWMMMKRGVEVEAVYFHSPPYTSDRAKEKVVDLCKVLSQYGRGMRLHVVHFT
CCCCCHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEE
DLQLEIYEKCPARLTTIIMRRMMMKIAEKIAQQNGSLALITGESLGQVASQTIESLYVTN
CCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHEEEC
SSVSLPVFRPLIGMDKREIIDIAQKIGTYEISIRPYEDCCTIFVPKHPATKPKLEKVLEA
CCCCCHHHHHHHCCCHHHHHHHHHHCCCEEEEEEEHHCCEEEEECCCCCCCHHHHHHHHH
EEKMDYQKFIDNFEEEVIEI
HHHCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKDVLLIKYGELALKGENRSFFENTLVKNIKYALRDFDGVKVEKTHGRIYVECEKDVEEA
CCCEEEEEECCEEEECCCCHHHHHHHHHHHHHHHHCCCCCEEEECCCEEEEEECHHHHHH
IERLKKVFGIVGITRAKEADLDLEEIFKAAVDLMKSHQGKTFKVETKRPNKAFPYNSMEV
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCHHHH
SRRVGAAVLKNVKNMKVDVHNPDVLLNVEIREKAFLYAGVIEGAGGLPLGTNGKATVLLS
HHHHHHHHHHHHHHCEEEECCCCEEEEEEEHHHHEEEEEHCCCCCCCCCCCCCCEEEEEE
GGIDSPVAAWMMMKRGVEVEAVYFHSPPYTSDRAKEKVVDLCKVLSQYGRGMRLHVVHFT
CCCCCHHHHHHHHHCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEEE
DLQLEIYEKCPARLTTIIMRRMMMKIAEKIAQQNGSLALITGESLGQVASQTIESLYVTN
CCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHEEEC
SSVSLPVFRPLIGMDKREIIDIAQKIGTYEISIRPYEDCCTIFVPKHPATKPKLEKVLEA
CCCCCHHHHHHHCCCHHHHHHHHHHCCCEEEEEEEHHCCEEEEECCCCCCCHHHHHHHHH
EEKMDYQKFIDNFEEEVIEI
HHHCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336