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The map label for this gene is NifS [H]

Identifier: 20808090

GI number: 20808090

Start: 1613571

End: 1614746

Strand: Reverse

Name: NifS [H]

Synonym: TTE1663

Alternate gene names: 20808090

Gene position: 1614746-1613571 (Counterclockwise)

Preceding gene: 20808091

Following gene: 20808089

Centisome position: 60.04

GC content: 39.37

Gene sequence:

>1176_bases
TTGCAAAATAAAGGGATGATGATTATGGAAGTGTATTTGGACAACAGCGCTACCACAAAAGTGAGAAAAGAAGTCATAGA
CAAAATGCTTGAAGTTTTAGAAGAGGAATATGGTAATCCTTCTTCACTTCACAGAAAGGGTTATCAAGCAGAAAAGCTCT
TAAAAGAAGCGAGAGAAAATGTTGCGTGTCTTATAGGCGGCAGACCAGAAGGCATTATATTTACTTCTGGCGGGACAGAG
TCTAATAACCTTGCTCTCATAGGAGTGGCAGAAAGCCTCAAGAAAAAGGGCAATCACATAATTTCTTCTACCATAGAACA
TCCTTCAATTTTAAATGTGCTTAAATTTTTAGAAGAAAGTGGGTTTAAAGTTACTTACATTGAAGTGGACAAAAAGGGAA
AGGTTAATCCTGAGGATGTTGAGAATGCTATTAACGATAAAACTATACTCATCTCAATTATGGCAGTAAATAACGAAATA
GGTACCATAGAGCCTATTGAGGAGATAGCAAGTATAGCGAAGAGAAATGGCGTTTTATTTCACGTAGATGCTGTACAAGC
TGCTGGCAAGATAGAGATTGACGTAAAGAAACAAAAGCTTGACATGGTGTCTTTAAGTGCGCATAAGATTCACGGTCCAA
AAGGAGTAGGGGCGCTATATTTATCGGAGGAAGTGAGAATAAGACCCATAATATTTGGAGGAGGGCAGGAAAAGAACATA
AGGTCTGGCACAGAAAATATGCCCGGTATTGTAGGATTTGGAGTTGCTAGCAGACTGGCCAAAGAAAGTTTAGAAGAAGT
TTCTCAGAAGCTCATGGGGTTAAAAAAGAGGTTGTGGGAGGGTATTTCTTCAGAAATAAAAGATGTGCATTTGAATGGAC
CTGAAGTAGAAGAAGGCGCGCCGCATATACTTAACGTTTCTTTCCGTGGAGTCAGAGGAGAGGTGCTTTTACACGCTTTA
GAAGAAGAGGGTATTTACGTCTCCACTGGATCTGCTTGTTCATCGAAAAAGAGAGGAGGAAGTCACGTCCTTAAAGCAAT
AGGCTTAAAAGGAGACCTGCTAGAAGGGGCTATCCGCTTTTCTCTGGGGATTTTTAATACTGAGGAAGACATAGATTACA
CAGTGGATATTTTGAAGAAAAAAGTTGACTTTTTAAGAAGATTTAAACGGAGGTAA

Upstream 100 bases:

>100_bases
TCAATAATACAGGAGCATGCAAAAGACTGTATACTTGCCAGTGAAGATGGAAAGGCTACTTATGAAGATGTGCAAAGAAT
TATTAACCTTTTAGTTAAAT

Downstream 100 bases:

>100_bases
AATAATGAAAGACGTACTTCTCATAAAATACGGAGAATTGGCATTGAAAGGAGAAAACAGGTCTTTTTTTGAAAATACAC
TTGTAAAAAATATAAAATAC

Product: cysteine sulfinate desulfinase/cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 391; Mature: 391

Protein sequence:

>391_residues
MQNKGMMIMEVYLDNSATTKVRKEVIDKMLEVLEEEYGNPSSLHRKGYQAEKLLKEARENVACLIGGRPEGIIFTSGGTE
SNNLALIGVAESLKKKGNHIISSTIEHPSILNVLKFLEESGFKVTYIEVDKKGKVNPEDVENAINDKTILISIMAVNNEI
GTIEPIEEIASIAKRNGVLFHVDAVQAAGKIEIDVKKQKLDMVSLSAHKIHGPKGVGALYLSEEVRIRPIIFGGGQEKNI
RSGTENMPGIVGFGVASRLAKESLEEVSQKLMGLKKRLWEGISSEIKDVHLNGPEVEEGAPHILNVSFRGVRGEVLLHAL
EEEGIYVSTGSACSSKKRGGSHVLKAIGLKGDLLEGAIRFSLGIFNTEEDIDYTVDILKKKVDFLRRFKRR

Sequences:

>Translated_391_residues
MQNKGMMIMEVYLDNSATTKVRKEVIDKMLEVLEEEYGNPSSLHRKGYQAEKLLKEARENVACLIGGRPEGIIFTSGGTE
SNNLALIGVAESLKKKGNHIISSTIEHPSILNVLKFLEESGFKVTYIEVDKKGKVNPEDVENAINDKTILISIMAVNNEI
GTIEPIEEIASIAKRNGVLFHVDAVQAAGKIEIDVKKQKLDMVSLSAHKIHGPKGVGALYLSEEVRIRPIIFGGGQEKNI
RSGTENMPGIVGFGVASRLAKESLEEVSQKLMGLKKRLWEGISSEIKDVHLNGPEVEEGAPHILNVSFRGVRGEVLLHAL
EEEGIYVSTGSACSSKKRGGSHVLKAIGLKGDLLEGAIRFSLGIFNTEEDIDYTVDILKKKVDFLRRFKRR
>Mature_391_residues
MQNKGMMIMEVYLDNSATTKVRKEVIDKMLEVLEEEYGNPSSLHRKGYQAEKLLKEARENVACLIGGRPEGIIFTSGGTE
SNNLALIGVAESLKKKGNHIISSTIEHPSILNVLKFLEESGFKVTYIEVDKKGKVNPEDVENAINDKTILISIMAVNNEI
GTIEPIEEIASIAKRNGVLFHVDAVQAAGKIEIDVKKQKLDMVSLSAHKIHGPKGVGALYLSEEVRIRPIIFGGGQEKNI
RSGTENMPGIVGFGVASRLAKESLEEVSQKLMGLKKRLWEGISSEIKDVHLNGPEVEEGAPHILNVSFRGVRGEVLLHAL
EEEGIYVSTGSACSSKKRGGSHVLKAIGLKGDLLEGAIRFSLGIFNTEEDIDYTVDILKKKVDFLRRFKRR

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=385, Percent_Identity=43.6363636363636, Blast_Score=317, Evalue=1e-86,
Organism=Homo sapiens, GI156713448, Length=412, Percent_Identity=36.1650485436893, Blast_Score=226, Evalue=4e-59,
Organism=Escherichia coli, GI48994898, Length=385, Percent_Identity=47.012987012987, Blast_Score=346, Evalue=2e-96,
Organism=Escherichia coli, GI1787970, Length=401, Percent_Identity=26.9326683291771, Blast_Score=116, Evalue=3e-27,
Organism=Escherichia coli, GI1789175, Length=402, Percent_Identity=23.3830845771144, Blast_Score=103, Evalue=1e-23,
Organism=Caenorhabditis elegans, GI25143064, Length=380, Percent_Identity=44.7368421052632, Blast_Score=314, Evalue=6e-86,
Organism=Caenorhabditis elegans, GI17533177, Length=317, Percent_Identity=34.384858044164, Blast_Score=162, Evalue=2e-40,
Organism=Saccharomyces cerevisiae, GI6319831, Length=388, Percent_Identity=43.0412371134021, Blast_Score=311, Evalue=8e-86,
Organism=Drosophila melanogaster, GI20129463, Length=379, Percent_Identity=44.5910290237467, Blast_Score=307, Evalue=6e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR017772
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 42994; Mature: 42994

Theoretical pI: Translated: 7.37; Mature: 7.37

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQNKGMMIMEVYLDNSATTKVRKEVIDKMLEVLEEEYGNPSSLHRKGYQAEKLLKEAREN
CCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHCC
VACLIGGRPEGIIFTSGGTESNNLALIGVAESLKKKGNHIISSTIEHPSILNVLKFLEES
EEEEECCCCCEEEEECCCCCCCCEEEEEEHHHHHHCCCEEHHHHCCCHHHHHHHHHHHHC
GFKVTYIEVDKKGKVNPEDVENAINDKTILISIMAVNNEIGTIEPIEEIASIAKRNGVLF
CCEEEEEEECCCCCCCHHHHHHHCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHCCEEE
HVDAVQAAGKIEIDVKKQKLDMVSLSAHKIHGPKGVGALYLSEEVRIRPIIFGGGQEKNI
EEECCCCCCEEEEEEEHHHHCEEEECHHCCCCCCCCEEEEECCCCEEEEEEECCCCCCCC
RSGTENMPGIVGFGVASRLAKESLEEVSQKLMGLKKRLWEGISSEIKDVHLNGPEVEEGA
CCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCCCCCC
PHILNVSFRGVRGEVLLHALEEEGIYVSTGSACSSKKRGGSHVLKAIGLKGDLLEGAIRF
CCEEEEEECCCCHHHHHHHHHHCCEEEECCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHH
SLGIFNTEEDIDYTVDILKKKVDFLRRFKRR
EEEECCCCCCCHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MQNKGMMIMEVYLDNSATTKVRKEVIDKMLEVLEEEYGNPSSLHRKGYQAEKLLKEAREN
CCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHCC
VACLIGGRPEGIIFTSGGTESNNLALIGVAESLKKKGNHIISSTIEHPSILNVLKFLEES
EEEEECCCCCEEEEECCCCCCCCEEEEEEHHHHHHCCCEEHHHHCCCHHHHHHHHHHHHC
GFKVTYIEVDKKGKVNPEDVENAINDKTILISIMAVNNEIGTIEPIEEIASIAKRNGVLF
CCEEEEEEECCCCCCCHHHHHHHCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHCCEEE
HVDAVQAAGKIEIDVKKQKLDMVSLSAHKIHGPKGVGALYLSEEVRIRPIIFGGGQEKNI
EEECCCCCCEEEEEEEHHHHCEEEECHHCCCCCCCCEEEEECCCCEEEEEEECCCCCCCC
RSGTENMPGIVGFGVASRLAKESLEEVSQKLMGLKKRLWEGISSEIKDVHLNGPEVEEGA
CCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEECCCCCCCCCC
PHILNVSFRGVRGEVLLHALEEEGIYVSTGSACSSKKRGGSHVLKAIGLKGDLLEGAIRF
CCEEEEEECCCCHHHHHHHHHHCCEEEECCCCCCCCCCCHHHHHHHHCCCCHHHHHHHHH
SLGIFNTEEDIDYTVDILKKKVDFLRRFKRR
EEEECCCCCCCHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA