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The map label for this gene is tnaA

Identifier: 20808031

GI number: 20808031

Start: 1558162

End: 1559550

Strand: Reverse

Name: tnaA

Synonym: TTE1602

Alternate gene names: 20808031

Gene position: 1559550-1558162 (Counterclockwise)

Preceding gene: 20808032

Following gene: 20808030

Centisome position: 57.99

GC content: 42.26

Gene sequence:

>1389_bases
GTGTACGATGATTTGACGAGAATGGCTGAGCCTTATAAGATCAAAATGGTGGAGCCGTTGAAAATTACCACGAGGGAAGA
GAGAATAAGGCTTATCAAAGAAGCCGGGTATAACTTGTTTTTATTAAAATCTGACGACGTGTACATTGACCTTTTGACTG
ATAGCGGCACTGCTGCGATGAGCGATTATCAATGGGCAGGTATGATGCTGGGGGATGAATCTTACGCAGGAAGCAGAAAC
TATCAGCATTTGTGCGAAGTTGTTCAAGATATTTTTGGCTATAAGTACGTTGTGCCAACCCATCAAGGGCGCGGAGCTGA
AAAGGTGTTGTTTCCTCTTCTCATAAAGAAGGGACAGTATGTACTTGGTAACATGCATTTTGATACCACAAAGGCCCATA
TTGAGCTAGCAGGCGGAATTGGTATAGACATGGTGATAAAGGAAGCTGAGGACACAGAAAACTACCATCCTTTTAAAGGA
AATTTTGATGTTGAAGCTTTGGAAAATTTCATAAAAGAAAAAGGTGCTGAAAACATAGCTTTTATTTTAGCAACGGTGAC
CTGCAACAGTGCGGGTGGACAGCCTGTTTCAATGGAAAACATAAAAGAAGTGAGGAGAATTGCTGATAAGTACGGTATCA
GGCTTTTCTTTGATGCAGCAAGATTTGCAGAGAATGCCTATTTCATAAAACAGAGAGAAAAAGGCTATGAAAACAAATCC
ATTAAAGAAATAGTAAGAGAAATGTTCTCCTACGGCGATGGCTTCACAATGTCTGCCAAAAAAGATGCGCTGGTAAACAT
CGGCGGCCTTATCGCTATAAAGGAGGATGAGGAGCTTTTTACAAGGGTTAAGACGATGCTCATACCTATGGAAGGTTTTG
TAACATACGGCGGGCTCGCTGGGAGAGACATGGAGGCTATGGCGAGAGGCTTAGAAGAAGTTCTTGATGAAAATTACCTT
GCTTGGAGAATAAATCAGGTAAAATATTTGGGGGACAAGTTGAGAGAGGCAGGCATACCGATTCAGTACCCGACTGGCGG
TCATGCTGTATTTATAGATGGCAAAAAGTTTTTGCCTCACGTGCCTCAGGAACAATTTCCTTCCCACGCAATTTGCGTAG
AACTTTATATAGAAGCAGGAATTAGGGCTGTAGAAGTAGGTGCTCTTCTAGCAGGAAGGGACCCAGAGACAGGTCAAAAC
AGGCTTCCAAAACTTGATTTTGTCCGACTTACCATTCCTAGGAGAGTTTACACGAACAGTCACATGGACGTGGTAGCAGA
AGCAGTGAAGAGGGTATATGCTAGAAGAGACCAAATAAAGGGTTATAGGTTTGTATATGAACCTCCAATTTTGAGACATT
TCACTGCTAGATTAGAGCCTGTAGAATGA

Upstream 100 bases:

>100_bases
ATAGAGGTAGTTGCTCATAAGTAAGAAACTGTTTTTCTGTCTTTTTAAACTGCTATTAAACGATTACAGGCTGGGGATAA
AAATTTTAGGAGGGATTTCT

Downstream 100 bases:

>100_bases
GGATGGCGTTTGCCATCTTCTTTTTTTGTGATAAAATGAAAGAAGGAAAATAGAGAGATTTATAGAATATTTATTGATAG
GTGAGAAATTGTGAGGAGAA

Product: tryptophanase

Products: NA

Alternate protein names: L-tryptophan indole-lyase; TNase

Number of amino acids: Translated: 462; Mature: 462

Protein sequence:

>462_residues
MYDDLTRMAEPYKIKMVEPLKITTREERIRLIKEAGYNLFLLKSDDVYIDLLTDSGTAAMSDYQWAGMMLGDESYAGSRN
YQHLCEVVQDIFGYKYVVPTHQGRGAEKVLFPLLIKKGQYVLGNMHFDTTKAHIELAGGIGIDMVIKEAEDTENYHPFKG
NFDVEALENFIKEKGAENIAFILATVTCNSAGGQPVSMENIKEVRRIADKYGIRLFFDAARFAENAYFIKQREKGYENKS
IKEIVREMFSYGDGFTMSAKKDALVNIGGLIAIKEDEELFTRVKTMLIPMEGFVTYGGLAGRDMEAMARGLEEVLDENYL
AWRINQVKYLGDKLREAGIPIQYPTGGHAVFIDGKKFLPHVPQEQFPSHAICVELYIEAGIRAVEVGALLAGRDPETGQN
RLPKLDFVRLTIPRRVYTNSHMDVVAEAVKRVYARRDQIKGYRFVYEPPILRHFTARLEPVE

Sequences:

>Translated_462_residues
MYDDLTRMAEPYKIKMVEPLKITTREERIRLIKEAGYNLFLLKSDDVYIDLLTDSGTAAMSDYQWAGMMLGDESYAGSRN
YQHLCEVVQDIFGYKYVVPTHQGRGAEKVLFPLLIKKGQYVLGNMHFDTTKAHIELAGGIGIDMVIKEAEDTENYHPFKG
NFDVEALENFIKEKGAENIAFILATVTCNSAGGQPVSMENIKEVRRIADKYGIRLFFDAARFAENAYFIKQREKGYENKS
IKEIVREMFSYGDGFTMSAKKDALVNIGGLIAIKEDEELFTRVKTMLIPMEGFVTYGGLAGRDMEAMARGLEEVLDENYL
AWRINQVKYLGDKLREAGIPIQYPTGGHAVFIDGKKFLPHVPQEQFPSHAICVELYIEAGIRAVEVGALLAGRDPETGQN
RLPKLDFVRLTIPRRVYTNSHMDVVAEAVKRVYARRDQIKGYRFVYEPPILRHFTARLEPVE
>Mature_462_residues
MYDDLTRMAEPYKIKMVEPLKITTREERIRLIKEAGYNLFLLKSDDVYIDLLTDSGTAAMSDYQWAGMMLGDESYAGSRN
YQHLCEVVQDIFGYKYVVPTHQGRGAEKVLFPLLIKKGQYVLGNMHFDTTKAHIELAGGIGIDMVIKEAEDTENYHPFKG
NFDVEALENFIKEKGAENIAFILATVTCNSAGGQPVSMENIKEVRRIADKYGIRLFFDAARFAENAYFIKQREKGYENKS
IKEIVREMFSYGDGFTMSAKKDALVNIGGLIAIKEDEELFTRVKTMLIPMEGFVTYGGLAGRDMEAMARGLEEVLDENYL
AWRINQVKYLGDKLREAGIPIQYPTGGHAVFIDGKKFLPHVPQEQFPSHAICVELYIEAGIRAVEVGALLAGRDPETGQN
RLPKLDFVRLTIPRRVYTNSHMDVVAEAVKRVYARRDQIKGYRFVYEPPILRHFTARLEPVE

Specific function: Tryptophan catabolism. [C]

COG id: COG3033

COG function: function code E; Tryptophanase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the beta-eliminating lyase family

Homologues:

Organism=Escherichia coli, GI87082323, Length=471, Percent_Identity=49.8938428874735, Blast_Score=466, Evalue=1e-132,

Paralogues:

None

Copy number: 7160 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 460 Molecules/Cell In: Early Stationary Phase, Rich Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): TNAA_THETN (Q8R9K5)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_623202.1
- ProteinModelPortal:   Q8R9K5
- SMR:   Q8R9K5
- GeneID:   996091
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE1602
- NMPDR:   fig|273068.3.peg.1579
- HOGENOM:   HBG297784
- OMA:   HAICVEL
- ProtClustDB:   PRK13238
- BioCyc:   TTEN273068:TTE1602-MONOMER
- BRENDA:   4.1.99.1
- HAMAP:   MF_00544
- InterPro:   IPR001597
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- InterPro:   IPR011166
- InterPro:   IPR018176
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PIRSF:   PIRSF001386

Pfam domain/function: PF01212 Beta_elim_lyase; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =4.1.99.1

Molecular weight: Translated: 52496; Mature: 52496

Theoretical pI: Translated: 6.21; Mature: 6.21

Prosite motif: PS00853 BETA_ELIM_LYASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYDDLTRMAEPYKIKMVEPLKITTREERIRLIKEAGYNLFLLKSDDVYIDLLTDSGTAAM
CCCCHHHCCCCEEEEEECCEEECCHHHHHHHHHHCCCEEEEEECCCEEEEEEECCCCCCC
SDYQWAGMMLGDESYAGSRNYQHLCEVVQDIFGYKYVVPTHQGRGAEKVLFPLLIKKGQY
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHCCCE
VLGNMHFDTTKAHIELAGGIGIDMVIKEAEDTENYHPFKGNFDVEALENFIKEKGAENIA
EEEEEEECCCEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEE
FILATVTCNSAGGQPVSMENIKEVRRIADKYGIRLFFDAARFAENAYFIKQREKGYENKS
EEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEEEEHHHHHCCHHEEEHHHCCCCCHH
IKEIVREMFSYGDGFTMSAKKDALVNIGGLIAIKEDEELFTRVKTMLIPMEGFVTYGGLA
HHHHHHHHHHCCCCEEECCCCCCEEECCEEEEEECCHHHHHHHHHHHCCCHHHEEECCCC
GRDMEAMARGLEEVLDENYLAWRINQVKYLGDKLREAGIPIQYPTGGHAVFIDGKKFLPH
CCCHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHCCCCEECCCCCEEEEECCHHHCCC
VPQEQFPSHAICVELYIEAGIRAVEVGALLAGRDPETGQNRLPKLDFVRLTIPRRVYTNS
CCHHHCCCCEEEEEEEEHHCCEEEEECCEEECCCCCCCCCCCCCCEEEEEEECCHHHCCC
HMDVVAEAVKRVYARRDQIKGYRFVYEPPILRHFTARLEPVE
HHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCC
>Mature Secondary Structure
MYDDLTRMAEPYKIKMVEPLKITTREERIRLIKEAGYNLFLLKSDDVYIDLLTDSGTAAM
CCCCHHHCCCCEEEEEECCEEECCHHHHHHHHHHCCCEEEEEECCCEEEEEEECCCCCCC
SDYQWAGMMLGDESYAGSRNYQHLCEVVQDIFGYKYVVPTHQGRGAEKVLFPLLIKKGQY
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHCCCE
VLGNMHFDTTKAHIELAGGIGIDMVIKEAEDTENYHPFKGNFDVEALENFIKEKGAENIA
EEEEEEECCCEEEEEEECCCCEEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCEE
FILATVTCNSAGGQPVSMENIKEVRRIADKYGIRLFFDAARFAENAYFIKQREKGYENKS
EEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCEEEEEEHHHHHCCHHEEEHHHCCCCCHH
IKEIVREMFSYGDGFTMSAKKDALVNIGGLIAIKEDEELFTRVKTMLIPMEGFVTYGGLA
HHHHHHHHHHCCCCEEECCCCCCEEECCEEEEEECCHHHHHHHHHHHCCCHHHEEECCCC
GRDMEAMARGLEEVLDENYLAWRINQVKYLGDKLREAGIPIQYPTGGHAVFIDGKKFLPH
CCCHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHCCCCEECCCCCEEEEECCHHHCCC
VPQEQFPSHAICVELYIEAGIRAVEVGALLAGRDPETGQNRLPKLDFVRLTIPRRVYTNS
CCHHHCCCCEEEEEEEEHHCCEEEEECCEEECCCCCCCCCCCCCCEEEEEEECCHHHCCC
HMDVVAEAVKRVYARRDQIKGYRFVYEPPILRHFTARLEPVE
HHHHHHHHHHHHHHHHHHCCCEEEEECCHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336