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The map label for this gene is NfnB2 [H]

Identifier: 20807986

GI number: 20807986

Start: 1516331

End: 1516951

Strand: Reverse

Name: NfnB2 [H]

Synonym: TTE1555

Alternate gene names: 20807986

Gene position: 1516951-1516331 (Counterclockwise)

Preceding gene: 20807991

Following gene: 20807985

Centisome position: 56.4

GC content: 35.27

Gene sequence:

>621_bases
ATGAAAGAATGCCAGAAACTTATAACAGAGAGAAGGTCTATCACATTTTTTGACACGACAAAAGAAATATCCGATGACCT
TATAAAAGAAGTCCTTGAGGTAGCTGCTACAGCGCCTTCAGCTAATAATCTTCAGCCTTGGGAGGTAAAAGTTATAAAGG
ACAAAGAAAAAAAGAAAATTCTCAAAGAAATCTGTTTTAACCAACAAAAAGTGGAAGATGCATCGGCTGTAATTGTCTTA
ATAGCAAATCCAAAAGCAGCTTTTGAACATATAGACAGAGTTTTAGACAGTTCTGTAGAACTGGGGTATATGCCAAAAGA
AAGGAGAGAATCAGCAAAAGAAAGGGTCCTTGAAGTGTGGAAAGACATGGAGCAAGCTAAAAGAAAGTCCATAAGAGATA
CTGCACTTTTTGCAATGAATATAATGATAGTTGCAAGAGTTTATGACCTTGAAACCCATCCCATGGATGGTTTTAATGAA
GAGAAAATGAAGGAGTTTTTAGGGATAGATGTAGAAAAAATAGTTCCCATGATAATAACGATAGGTTACAAGGACCCAAG
TAAAGATTTACTGCCAAGAGCCTATAGATTTAAATTTGATGAATTTGGAGAGATAATCTAA

Upstream 100 bases:

>100_bases
TATGGAGAATATAAATCTATTATCGGCATTTTTTAAAAACTGGCATACCATTTTTAGGTTAGTTTTGTAATTCTGGATTA
AAAAAGGAGAGTGTATAAGT

Downstream 100 bases:

>100_bases
TGAGTAATTACGTAATAATTTTAGTGGCTTCTATTTTAGCAGGAGCTACTAATGCAATAGCAGGAGGGGGTACTTTAATA
ACCTTTCCTGCTCTTGTATG

Product: nitroreductase

Products: NAD(P)(+); @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

Alternate protein names: NA

Number of amino acids: Translated: 206; Mature: 206

Protein sequence:

>206_residues
MKECQKLITERRSITFFDTTKEISDDLIKEVLEVAATAPSANNLQPWEVKVIKDKEKKKILKEICFNQQKVEDASAVIVL
IANPKAAFEHIDRVLDSSVELGYMPKERRESAKERVLEVWKDMEQAKRKSIRDTALFAMNIMIVARVYDLETHPMDGFNE
EKMKEFLGIDVEKIVPMIITIGYKDPSKDLLPRAYRFKFDEFGEII

Sequences:

>Translated_206_residues
MKECQKLITERRSITFFDTTKEISDDLIKEVLEVAATAPSANNLQPWEVKVIKDKEKKKILKEICFNQQKVEDASAVIVL
IANPKAAFEHIDRVLDSSVELGYMPKERRESAKERVLEVWKDMEQAKRKSIRDTALFAMNIMIVARVYDLETHPMDGFNE
EKMKEFLGIDVEKIVPMIITIGYKDPSKDLLPRAYRFKFDEFGEII
>Mature_206_residues
MKECQKLITERRSITFFDTTKEISDDLIKEVLEVAATAPSANNLQPWEVKVIKDKEKKKILKEICFNQQKVEDASAVIVL
IANPKAAFEHIDRVLDSSVELGYMPKERRESAKERVLEVWKDMEQAKRKSIRDTALFAMNIMIVARVYDLETHPMDGFNE
EKMKEFLGIDVEKIVPMIITIGYKDPSKDLLPRAYRFKFDEFGEII

Specific function: Reduction Of A Variety Of Nitroaromatic Compounds Using NADH (And To Lesser Extent Nadph) As Source Of Reducing Equivalents; Two Electrons Are Transferred. Capable Of Reducing Nitrofurazone, Quinones And The Anti-Tumor Agent Cb1954 (5- (Aziridin-1-Yl)-2,4

COG id: COG0778

COG function: function code C; Nitroreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nitroreductase family [H]

Homologues:

None

Paralogues:

None

Copy number: 260 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000415 [H]

Pfam domain/function: PF00881 Nitroreductase [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 23916; Mature: 23916

Theoretical pI: Translated: 5.52; Mature: 5.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKECQKLITERRSITFFDTTKEISDDLIKEVLEVAATAPSANNLQPWEVKVIKDKEKKKI
CHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHH
LKEICFNQQKVEDASAVIVLIANPKAAFEHIDRVLDSSVELGYMPKERRESAKERVLEVW
HHHHHCCHHHCCCCCEEEEEEECCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
KDMEQAKRKSIRDTALFAMNIMIVARVYDLETHPMDGFNEEKMKEFLGIDVEKIVPMIIT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHE
IGYKDPSKDLLPRAYRFKFDEFGEII
ECCCCCCHHHCCHHHHCCHHHHCCCC
>Mature Secondary Structure
MKECQKLITERRSITFFDTTKEISDDLIKEVLEVAATAPSANNLQPWEVKVIKDKEKKKI
CHHHHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCHHHHHH
LKEICFNQQKVEDASAVIVLIANPKAAFEHIDRVLDSSVELGYMPKERRESAKERVLEVW
HHHHHCCHHHCCCCCEEEEEEECCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHH
KDMEQAKRKSIRDTALFAMNIMIVARVYDLETHPMDGFNEEKMKEFLGIDVEKIVPMIIT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCHHHHHHHHHE
IGYKDPSKDLLPRAYRFKFDEFGEII
ECCCCCCHHHCCHHHHCCHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: FMN. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H; 6,7-Dihydropteridine [C]

Specific reaction: NAD(P)H + 6,7-Dihydropteridine = NAD(P)(+) + @TETR01.txt*5,6,7,8-Tetrahydropteridine!. [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]