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The map label for this gene is HsdM [H]

Identifier: 20807981

GI number: 20807981

Start: 1507342

End: 1508865

Strand: Reverse

Name: HsdM [H]

Synonym: TTE1547

Alternate gene names: 20807981

Gene position: 1508865-1507342 (Counterclockwise)

Preceding gene: 20807982

Following gene: 20807980

Centisome position: 56.1

GC content: 51.51

Gene sequence:

>1524_bases
ATGAACGGACCGCAAACTAGAGAATCTCTTGCCAATGAAATATGGCGTGCATGTGACATCATGCGCCGTGACAATAACTG
CACCGGTATCATGGAATACGTTGAACACCTAGCATGGTTACTTTTTTTACGCTTTTTAGACGCTCAGGAAGAAGAGTGGG
AAGCTCAGGCTCAAATTGCCGGACGCCCTTACACTCCTATTATTGACTCGGAGTACCGCTGGCGCCACTGGGCAACAAAA
GACTGGCCTGCTGATGAACTTTTGGCCTTCGTGCACGGCCGCCTCATCCCCTATCTCCGGAGCCTGGGCGGTGACCCGCT
GCGTGAAACTATCAGAAGCCTTTTTAGTGAACGTAACGTTATTGTCTGTGCTTCGGGCTACAACCTCAAAGACGTTATCC
AGATCGTAAACGAAATTAACTTTCACAGCCAAGACGATATTTTTACTGTTTCCCAGGTGTACGAAGAGCTTCTGCGCCGT
CTGGGGAACGAAAACCGCCTTGCGGGAGAGTTCTACACTCCGCGACCAGTGGTGCGGTTTGTCGTCGAACTAGTAGACCC
GCAAATAGGAGAGGCAGTCTACGACCCAGCCTGCGGGACTTGCGGCTTTCTGGTCGAGGCGTACCTCTGGATGAAGCAGA
AGGAACGAACCATAGAGGATCACCGCATACTACAGGAACGGACTTTTTTCGGCCAGGAGAAAAAGCCCGTTCCTGCGTTT
TTGGGTCTGGTAAATATGATGCTGCACGGTGTGACGGTACCACGTGTGATGCGTAGAAACACACTGGAAGAAAATATCCG
CAACGTCTCCGAGCGCTTTGACGTGGTGGTAACTAATCCGCCCTTTGGGGGAACAGAGGGACGGCACATCCAGCAAAACT
TCCCCATCCAGTCCAACGCAACAGAGCTCCTTTTTCTCCAGCATATTATGAAGAAGCTCAAACCCCGTGACGGAGCTCGT
TGCGGCATGGTTGTGCCGGAAGGAACTCTCTTTCGTGGAGGTGCTTTCGCCGAAGTTAAGCGGGATTTGCTGGAGCAATT
CAATTTGCACACAGTGGTGAGCCTTCCTCCGGGCACTTTTGCTCCTTATTCTGATGTGAAAACAGCCCTTATCTTTTTTG
AGCGTCCCGGGCCAACAAAGGAAATCTGGTATTACGAATTGCCTTTACCGGAAGGGCTGAAGAAGTTTAGCAAAGGCAAC
CCCATCCAGGATGAGCACTTTGAGGAAGCGCGCAAACTTTGGCGTGGCTGGGATGCCTACCGAAAGGGCTTGGGGCCCGT
TGAAGCCTGCCTTTCGGAACGATCCTGGATTGTGCCCGTGGAAGAGGTAAAAAAGCGGGGCTACGACCTCACTGCCCGCA
ACCCGAACCGCTCTGGAGGAGAAGAATTGCCGTCGCCGGTGGAAATCGTAGCCGGGCTTTTGGAAAAAGAGCGGGAGATT
TTAAGCATTATGGAGGAATTAAGTGAATTGTTAGAAAACGAGAAAGGCAACTCTAGCCGGAAGAACGACGAAGAGGAGGA
ATAA

Upstream 100 bases:

>100_bases
CTTTTACCATAAGCCGCTGGTTTGGCGGGCATGGGAGCTTACGCTCCACTCTTCTTACAATACAGCAAAAAATTTATCCA
GAGGAGGGAATTAGCTGATT

Downstream 100 bases:

>100_bases
GAAAGAATGGCTCGCAACTTCGCTCTTGTAAAAAGCATTGCTGAATTTCAAGGGCTAAGGCCAGAAGAAATTATGAGAGA
ATTGTATAATGGTTTCCAGG

Product: Type I restriction-modification system methyltransferase subunit

Products: NA

Alternate protein names: M.EcoEI [H]

Number of amino acids: Translated: 507; Mature: 507

Protein sequence:

>507_residues
MNGPQTRESLANEIWRACDIMRRDNNCTGIMEYVEHLAWLLFLRFLDAQEEEWEAQAQIAGRPYTPIIDSEYRWRHWATK
DWPADELLAFVHGRLIPYLRSLGGDPLRETIRSLFSERNVIVCASGYNLKDVIQIVNEINFHSQDDIFTVSQVYEELLRR
LGNENRLAGEFYTPRPVVRFVVELVDPQIGEAVYDPACGTCGFLVEAYLWMKQKERTIEDHRILQERTFFGQEKKPVPAF
LGLVNMMLHGVTVPRVMRRNTLEENIRNVSERFDVVVTNPPFGGTEGRHIQQNFPIQSNATELLFLQHIMKKLKPRDGAR
CGMVVPEGTLFRGGAFAEVKRDLLEQFNLHTVVSLPPGTFAPYSDVKTALIFFERPGPTKEIWYYELPLPEGLKKFSKGN
PIQDEHFEEARKLWRGWDAYRKGLGPVEACLSERSWIVPVEEVKKRGYDLTARNPNRSGGEELPSPVEIVAGLLEKEREI
LSIMEELSELLENEKGNSSRKNDEEEE

Sequences:

>Translated_507_residues
MNGPQTRESLANEIWRACDIMRRDNNCTGIMEYVEHLAWLLFLRFLDAQEEEWEAQAQIAGRPYTPIIDSEYRWRHWATK
DWPADELLAFVHGRLIPYLRSLGGDPLRETIRSLFSERNVIVCASGYNLKDVIQIVNEINFHSQDDIFTVSQVYEELLRR
LGNENRLAGEFYTPRPVVRFVVELVDPQIGEAVYDPACGTCGFLVEAYLWMKQKERTIEDHRILQERTFFGQEKKPVPAF
LGLVNMMLHGVTVPRVMRRNTLEENIRNVSERFDVVVTNPPFGGTEGRHIQQNFPIQSNATELLFLQHIMKKLKPRDGAR
CGMVVPEGTLFRGGAFAEVKRDLLEQFNLHTVVSLPPGTFAPYSDVKTALIFFERPGPTKEIWYYELPLPEGLKKFSKGN
PIQDEHFEEARKLWRGWDAYRKGLGPVEACLSERSWIVPVEEVKKRGYDLTARNPNRSGGEELPSPVEIVAGLLEKEREI
LSIMEELSELLENEKGNSSRKNDEEEE
>Mature_507_residues
MNGPQTRESLANEIWRACDIMRRDNNCTGIMEYVEHLAWLLFLRFLDAQEEEWEAQAQIAGRPYTPIIDSEYRWRHWATK
DWPADELLAFVHGRLIPYLRSLGGDPLRETIRSLFSERNVIVCASGYNLKDVIQIVNEINFHSQDDIFTVSQVYEELLRR
LGNENRLAGEFYTPRPVVRFVVELVDPQIGEAVYDPACGTCGFLVEAYLWMKQKERTIEDHRILQERTFFGQEKKPVPAF
LGLVNMMLHGVTVPRVMRRNTLEENIRNVSERFDVVVTNPPFGGTEGRHIQQNFPIQSNATELLFLQHIMKKLKPRDGAR
CGMVVPEGTLFRGGAFAEVKRDLLEQFNLHTVVSLPPGTFAPYSDVKTALIFFERPGPTKEIWYYELPLPEGLKKFSKGN
PIQDEHFEEARKLWRGWDAYRKGLGPVEACLSERSWIVPVEEVKKRGYDLTARNPNRSGGEELPSPVEIVAGLLEKEREI
LSIMEELSELLENEKGNSSRKNDEEEE

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0286

COG function: function code V; Type I restriction-modification system methyltransferase subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

Organism=Escherichia coli, GI1790808, Length=460, Percent_Identity=29.5652173913043, Blast_Score=177, Evalue=1e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022749
- InterPro:   IPR003356
- InterPro:   IPR002052
- InterPro:   IPR002296 [H]

Pfam domain/function: PF12161 HsdM_N; PF02384 N6_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 58495; Mature: 58495

Theoretical pI: Translated: 5.11; Mature: 5.11

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNGPQTRESLANEIWRACDIMRRDNNCTGIMEYVEHLAWLLFLRFLDAQEEEWEAQAQIA
CCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHC
GRPYTPIIDSEYRWRHWATKDWPADELLAFVHGRLIPYLRSLGGDPLRETIRSLFSERNV
CCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCE
IVCASGYNLKDVIQIVNEINFHSQDDIFTVSQVYEELLRRLGNENRLAGEFYTPRPVVRF
EEEECCCCHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
VVELVDPQIGEAVYDPACGTCGFLVEAYLWMKQKERTIEDHRILQERTFFGQEKKPVPAF
HHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
LGLVNMMLHGVTVPRVMRRNTLEENIRNVSERFDVVVTNPPFGGTEGRHIQQNFPIQSNA
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCC
TELLFLQHIMKKLKPRDGARCGMVVPEGTLFRGGAFAEVKRDLLEQFNLHTVVSLPPGTF
HHHHHHHHHHHHCCCCCCCCCCEECCCCCEECCCCHHHHHHHHHHHCCCCEEEECCCCCC
APYSDVKTALIFFERPGPTKEIWYYELPLPEGLKKFSKGNPIQDEHFEEARKLWRGWDAY
CCHHHHCEEEEEEECCCCCCEEEEEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
RKGLGPVEACLSERSWIVPVEEVKKRGYDLTARNPNRSGGEELPSPVEIVAGLLEKEREI
HHCCCHHHHHHCCCCCCCCHHHHHHCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHH
LSIMEELSELLENEKGNSSRKNDEEEE
HHHHHHHHHHHHCCCCCCCCCCCCCCC
>Mature Secondary Structure
MNGPQTRESLANEIWRACDIMRRDNNCTGIMEYVEHLAWLLFLRFLDAQEEEWEAQAQIA
CCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHC
GRPYTPIIDSEYRWRHWATKDWPADELLAFVHGRLIPYLRSLGGDPLRETIRSLFSERNV
CCCCCCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCE
IVCASGYNLKDVIQIVNEINFHSQDDIFTVSQVYEELLRRLGNENRLAGEFYTPRPVVRF
EEEECCCCHHHHHHHHHHCCCCCCCCCEEHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHH
VVELVDPQIGEAVYDPACGTCGFLVEAYLWMKQKERTIEDHRILQERTFFGQEKKPVPAF
HHHHHCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
LGLVNMMLHGVTVPRVMRRNTLEENIRNVSERFDVVVTNPPFGGTEGRHIQQNFPIQSNA
HHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCCCCCCCCCCCCC
TELLFLQHIMKKLKPRDGARCGMVVPEGTLFRGGAFAEVKRDLLEQFNLHTVVSLPPGTF
HHHHHHHHHHHHCCCCCCCCCCEECCCCCEECCCCHHHHHHHHHHHCCCCEEEECCCCCC
APYSDVKTALIFFERPGPTKEIWYYELPLPEGLKKFSKGNPIQDEHFEEARKLWRGWDAY
CCHHHHCEEEEEEECCCCCCEEEEEECCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHH
RKGLGPVEACLSERSWIVPVEEVKKRGYDLTARNPNRSGGEELPSPVEIVAGLLEKEREI
HHCCCHHHHHHCCCCCCCCHHHHHHCCCCEECCCCCCCCCCCCCCHHHHHHHHHHHHHHH
LSIMEELSELLENEKGNSSRKNDEEEE
HHHHHHHHHHHHCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8412658 [H]