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The map label for this gene is LigT [C]

Identifier: 20807960

GI number: 20807960

Start: 1483003

End: 1483551

Strand: Reverse

Name: LigT [C]

Synonym: TTE1518

Alternate gene names: 20807960

Gene position: 1483551-1483003 (Counterclockwise)

Preceding gene: 20807962

Following gene: 20807958

Centisome position: 55.16

GC content: 32.79

Gene sequence:

>549_bases
ATGAGAGCATTTATTGGAATAGGGATGCAAACAGAAATAATAGAGAAAATCTACGAATTTCAGTCTATGCTTAGAGAATA
CACTGTAGAAGGAAAGTGGACTAATAAAGAAAATTTTCACATTACATTGAAATTTTTGGGAGAAATACAACCAGAGATTG
TCCCTCAAATAGAAGAAGCAGTAAAAAAAGCAACAAAAGGAATAAAGCCTTTCTATCTCCGTTTTTCAGAAGTGGGTTTT
TTTAAAGGAAACAAAAATATCAGGGTTTTATGGTTAGGAACTGAGGAAAGTAAACCTTTAAATGTTTTGCATGCTAATAT
AGAAGATGAGCTTTACAAGATTGGTTTTCCCAAAGAAGAAAGAAAATTCACTTCTCACATTACCATAGCGAGAGATATTA
AGCTAAAAATTGGAATTGAAGAGGCAAATAAGCTTTTCAGCCAAATAGAAAAAAAGGATATCCCTGTAAAGGAGATATCT
CTTTATGAAAGCGCTGTTATTGAGGGCAAAAGAAAATACATACCAGTTTTCACTGTGCCCCTTAAATGA

Upstream 100 bases:

>100_bases
GAAAGCCAAATTACCTCTTTTCTTTTTATGGTATAATTATAGAGTGACATCCGAGCAAATTTCCTGGGAAATAATAACTT
TAAAGGAAGGATAGAGGGAA

Downstream 100 bases:

>100_bases
GCTCACTCAGGATTTACGTAGATGGTTTTGTGGTTAGTGTAAATTACAAACCCCGGTTTTGAACCTGGGGGTTTTTTTAC
ATACTTTTTTAAGGTATAAT

Product: 3'-5' RNA ligase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 182; Mature: 182

Protein sequence:

>182_residues
MRAFIGIGMQTEIIEKIYEFQSMLREYTVEGKWTNKENFHITLKFLGEIQPEIVPQIEEAVKKATKGIKPFYLRFSEVGF
FKGNKNIRVLWLGTEESKPLNVLHANIEDELYKIGFPKEERKFTSHITIARDIKLKIGIEEANKLFSQIEKKDIPVKEIS
LYESAVIEGKRKYIPVFTVPLK

Sequences:

>Translated_182_residues
MRAFIGIGMQTEIIEKIYEFQSMLREYTVEGKWTNKENFHITLKFLGEIQPEIVPQIEEAVKKATKGIKPFYLRFSEVGF
FKGNKNIRVLWLGTEESKPLNVLHANIEDELYKIGFPKEERKFTSHITIARDIKLKIGIEEANKLFSQIEKKDIPVKEIS
LYESAVIEGKRKYIPVFTVPLK
>Mature_182_residues
MRAFIGIGMQTEIIEKIYEFQSMLREYTVEGKWTNKENFHITLKFLGEIQPEIVPQIEEAVKKATKGIKPFYLRFSEVGF
FKGNKNIRVLWLGTEESKPLNVLHANIEDELYKIGFPKEERKFTSHITIARDIKLKIGIEEANKLFSQIEKKDIPVKEIS
LYESAVIEGKRKYIPVFTVPLK

Specific function: Specifically Ligates tRNA Half-Molecules Containing Nucleoside Base Modifications And Shows A Preference Among Different tRNA Species. Acts In The Absence Of ATP To Form A 2'-5' Phosphodiester Linkage. [C]

COG id: COG1514

COG function: function code J; 2'-5' RNA ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the UPF0097 family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004175
- InterPro:   IPR014051
- InterPro:   IPR009097 [H]

Pfam domain/function: PF02834 2_5_RNA_ligase [H]

EC number: 6.5.1.- [C]

Molecular weight: Translated: 21223; Mature: 21223

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRAFIGIGMQTEIIEKIYEFQSMLREYTVEGKWTNKENFHITLKFLGEIQPEIVPQIEEA
CCEEEECCCHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEEEEEECCCCCHHHHHHHHH
VKKATKGIKPFYLRFSEVGFFKGNKNIRVLWLGTEESKPLNVLHANIEDELYKIGFPKEE
HHHHHCCCCCEEEEEEHEEEEECCCCEEEEEEECCCCCCEEEEECCCCHHEEECCCCHHH
RKFTSHITIARDIKLKIGIEEANKLFSQIEKKDIPVKEISLYESAVIEGKRKYIPVFTVP
HHHHHEEEEEEEEEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEEEE
LK
CC
>Mature Secondary Structure
MRAFIGIGMQTEIIEKIYEFQSMLREYTVEGKWTNKENFHITLKFLGEIQPEIVPQIEEA
CCEEEECCCHHHHHHHHHHHHHHHHHHCCCCEECCCCCEEEEEEEECCCCCHHHHHHHHH
VKKATKGIKPFYLRFSEVGFFKGNKNIRVLWLGTEESKPLNVLHANIEDELYKIGFPKEE
HHHHHCCCCCEEEEEEHEEEEECCCCEEEEEEECCCCCCEEEEECCCCHHEEECCCCHHH
RKFTSHITIARDIKLKIGIEEANKLFSQIEKKDIPVKEISLYESAVIEGKRKYIPVFTVP
HHHHHEEEEEEEEEEEECHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEEEEEEEE
LK
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9679194 [H]