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The map label for this gene is PriA [H]

Identifier: 20807951

GI number: 20807951

Start: 1473051

End: 1475249

Strand: Reverse

Name: PriA [H]

Synonym: TTE1508

Alternate gene names: 20807951

Gene position: 1475249-1473051 (Counterclockwise)

Preceding gene: 20807952

Following gene: 20807950

Centisome position: 54.85

GC content: 38.7

Gene sequence:

>2199_bases
ATGTTCGCCGAAGTCATAGTGGAAATAAAAGCTTCGAATACTGACAGGCTTTATACTTACAAAGTGCCAGAAGGAATGGA
CCTTGAAGTAGGTATGAGGGTTTTAGTGCCTTTCAAAAATAGGCTTGTTGAGGGCTATGTGGTAAGCCTTACGGATAAGC
CTTCTTATTCGCTAGAAAAGATAAAGAGCGTGTACAAAATTTTAGATAAATATCCTATTTTTAACGAGAAAATGATAAAG
TTGGCAAAATGGATGAGAGATTATTATAAATGCTATTTTTCTGAAGTTTTTCAGACGATAATGCCGGCAGGAATAGACAG
AGGAGAAAAAAAGGTAAAGCTGGTCAGATTGAAGATAGAAGATGTTTTTGAGGAGGATTTTAAAAAGGCTCCAAGGCAGT
ATGAAGTCTTGCGGTATCTTAAAAAGAGAAGTCCTATAAAGCTTTCCGAACTTGTAAAGATATTAAATACGGATTATGGT
ACTATAAGGGCTCTTGAGAAAAAGGGTTATATAGAGGTTTATGAAGAGGAAATATCGAGATTTCAGGTGAGAGATGTAGA
AAAGACCTTTCCTCCAATTTTGACAGAAGAACAGAAAAGAGCAGTTGAGGAGGTAAAAAAATCTATTTTCTCAGGACAGC
CTGATGCATACTTGCTTTTTGGAGTTACTGGCAGTGGAAAGACAGAGGTTTACTTAAAGCTCATAGAAGAAACTGTGAAG
ATAGGAAAGAGCGCAATAGTCCTTGTGCCAGAAATAGCTTTAACGCCTCAGACAATTGAGAGGTTTGTAAGCCGCTTTGG
CAATAGAGTTGCTGTGATTCACAGCGGGCTTTCTCCGGGAGAAAGGTTTGATGAATGGCGAAAAGTAAAAAATAAAGAAG
TGGACGTAGTAGTAGGGGTAAGGAGTGCAGTTTTTGCGCCTTTTGACAACCTTGGACTTATAATTATTGATGAGGAACAT
GAAGCTACTTACAAGCAGTCAGATACAAGGCCCCGGTACAACGCAAAAGAAGTAGCAGAAAAGAGATGTGAAATAGAAAA
AGCTGTATTGGTCATGGCAAGCGCTACTCCTTCTTTGGAAACCTATTACAAAGCCATAAAGGGCGATTACAAGCTTTTGA
AGCTTACCAGAAGAATAAAAGCCTCTATGCCTGAGATAGAAGTTGTCAATATGAGCGATGAGTTAGCAAAAGGCAACAAT
TCTATCTTTAGCAGAAAGTTGTTTTCCTATATCAAAGAAAATTTGAAAAAAGGTGAGCAGACAATTTTGTTTTTAAACAG
AAGAGGGTATTCCTCTTTTGTGGCTTGCAGAGACTGCGGTTACGTTCCAAAATGCCCTAACTGCGACATATCTTTGACAT
ATCACTTTGAGGATAAGAAACTGGTGTGTCATTACTGCGGCTATGAGGAGCCTATGAGGGAGACATGTCCTAAGTGCGGT
AGCAAAAGGATACGGTATATGGGTATCGGCACGGAAAGGGTAGAGATGGATATAAAGAAATTCTTTCCAGAAGCTAGAGT
TTTAAGGATGGATGTAGATACTACCAGGAGAAAGGGGTCCCATGAGAAGATATTTTACGAGTTTAAAAATGGGAAAGCAG
ATATTTTGATAGGGACACAGATGATATCAAAAGGATTTGACATACCTAATGTGACTTTAGTAGGAGTGATACTCGCTGAT
ATAACATTGAACTTGCCAGATTTTAGGTCTAGTGAGAGGACTTTTCAACTGCTAACGCAGGTGGCAGGAAGAGCAGGAAG
GGGAGAAAAGCCCGGAAGAGTGGTGATACAGACATATGAGGATAATCATTACAGCATCCTCACGGCAAAAGAACAGGACT
ATGTAAAATTTTTCAGGGAAGAGATAAAATATAGAGAAATTTTTAAGTACCCTCCTTTTGCACACCTTATGAATATTGTA
GTATCAGGGGAAGACGAAGAAAAAGTGAAAAATGCCTCTGCAAACACATATTTAGCATGTCAGAAAGTTATTAACAAACT
GCAAGTTAAGAGTTATAATAAAATACTAGGGCCTGCCCCTGCTCCTATAAGCAAGATTAACAACCGATACAGGTGGCAGG
TGATTGTTAAAAGTGAGGAGAGAGAGGTGCTAGAAGAAATTGCAGAGTATGTCCAGAATATGAAATATCCAAAAGACATA
AGGGTTGCTGTAGACATTGACCCACTTAATATAATGTAA

Upstream 100 bases:

>100_bases
AGGGAAGCAGCTCATGTCATTTTAGATGAGATAGCAAAGTTGTTGTGAGCCTTTTTCGGCTTTTTTTATTTATCTAAAAT
TTTATCGGGCGGAGGTTGCC

Downstream 100 bases:

>100_bases
TTTAAATTTAAGGAGGTATTTGTTTTGGCAATAAGGTATATTAGAAAAATTGGGGACCCCGTGCTCAGGAAAAAAGCTAA
ACCCGTTACTGAAATAAATT

Product: primosomal protein N

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 732; Mature: 732

Protein sequence:

>732_residues
MFAEVIVEIKASNTDRLYTYKVPEGMDLEVGMRVLVPFKNRLVEGYVVSLTDKPSYSLEKIKSVYKILDKYPIFNEKMIK
LAKWMRDYYKCYFSEVFQTIMPAGIDRGEKKVKLVRLKIEDVFEEDFKKAPRQYEVLRYLKKRSPIKLSELVKILNTDYG
TIRALEKKGYIEVYEEEISRFQVRDVEKTFPPILTEEQKRAVEEVKKSIFSGQPDAYLLFGVTGSGKTEVYLKLIEETVK
IGKSAIVLVPEIALTPQTIERFVSRFGNRVAVIHSGLSPGERFDEWRKVKNKEVDVVVGVRSAVFAPFDNLGLIIIDEEH
EATYKQSDTRPRYNAKEVAEKRCEIEKAVLVMASATPSLETYYKAIKGDYKLLKLTRRIKASMPEIEVVNMSDELAKGNN
SIFSRKLFSYIKENLKKGEQTILFLNRRGYSSFVACRDCGYVPKCPNCDISLTYHFEDKKLVCHYCGYEEPMRETCPKCG
SKRIRYMGIGTERVEMDIKKFFPEARVLRMDVDTTRRKGSHEKIFYEFKNGKADILIGTQMISKGFDIPNVTLVGVILAD
ITLNLPDFRSSERTFQLLTQVAGRAGRGEKPGRVVIQTYEDNHYSILTAKEQDYVKFFREEIKYREIFKYPPFAHLMNIV
VSGEDEEKVKNASANTYLACQKVINKLQVKSYNKILGPAPAPISKINNRYRWQVIVKSEEREVLEEIAEYVQNMKYPKDI
RVAVDIDPLNIM

Sequences:

>Translated_732_residues
MFAEVIVEIKASNTDRLYTYKVPEGMDLEVGMRVLVPFKNRLVEGYVVSLTDKPSYSLEKIKSVYKILDKYPIFNEKMIK
LAKWMRDYYKCYFSEVFQTIMPAGIDRGEKKVKLVRLKIEDVFEEDFKKAPRQYEVLRYLKKRSPIKLSELVKILNTDYG
TIRALEKKGYIEVYEEEISRFQVRDVEKTFPPILTEEQKRAVEEVKKSIFSGQPDAYLLFGVTGSGKTEVYLKLIEETVK
IGKSAIVLVPEIALTPQTIERFVSRFGNRVAVIHSGLSPGERFDEWRKVKNKEVDVVVGVRSAVFAPFDNLGLIIIDEEH
EATYKQSDTRPRYNAKEVAEKRCEIEKAVLVMASATPSLETYYKAIKGDYKLLKLTRRIKASMPEIEVVNMSDELAKGNN
SIFSRKLFSYIKENLKKGEQTILFLNRRGYSSFVACRDCGYVPKCPNCDISLTYHFEDKKLVCHYCGYEEPMRETCPKCG
SKRIRYMGIGTERVEMDIKKFFPEARVLRMDVDTTRRKGSHEKIFYEFKNGKADILIGTQMISKGFDIPNVTLVGVILAD
ITLNLPDFRSSERTFQLLTQVAGRAGRGEKPGRVVIQTYEDNHYSILTAKEQDYVKFFREEIKYREIFKYPPFAHLMNIV
VSGEDEEKVKNASANTYLACQKVINKLQVKSYNKILGPAPAPISKINNRYRWQVIVKSEEREVLEEIAEYVQNMKYPKDI
RVAVDIDPLNIM
>Mature_732_residues
MFAEVIVEIKASNTDRLYTYKVPEGMDLEVGMRVLVPFKNRLVEGYVVSLTDKPSYSLEKIKSVYKILDKYPIFNEKMIK
LAKWMRDYYKCYFSEVFQTIMPAGIDRGEKKVKLVRLKIEDVFEEDFKKAPRQYEVLRYLKKRSPIKLSELVKILNTDYG
TIRALEKKGYIEVYEEEISRFQVRDVEKTFPPILTEEQKRAVEEVKKSIFSGQPDAYLLFGVTGSGKTEVYLKLIEETVK
IGKSAIVLVPEIALTPQTIERFVSRFGNRVAVIHSGLSPGERFDEWRKVKNKEVDVVVGVRSAVFAPFDNLGLIIIDEEH
EATYKQSDTRPRYNAKEVAEKRCEIEKAVLVMASATPSLETYYKAIKGDYKLLKLTRRIKASMPEIEVVNMSDELAKGNN
SIFSRKLFSYIKENLKKGEQTILFLNRRGYSSFVACRDCGYVPKCPNCDISLTYHFEDKKLVCHYCGYEEPMRETCPKCG
SKRIRYMGIGTERVEMDIKKFFPEARVLRMDVDTTRRKGSHEKIFYEFKNGKADILIGTQMISKGFDIPNVTLVGVILAD
ITLNLPDFRSSERTFQLLTQVAGRAGRGEKPGRVVIQTYEDNHYSILTAKEQDYVKFFREEIKYREIFKYPPFAHLMNIV
VSGEDEEKVKNASANTYLACQKVINKLQVKSYNKILGPAPAPISKINNRYRWQVIVKSEEREVLEEIAEYVQNMKYPKDI
RVAVDIDPLNIM

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=739, Percent_Identity=35.8592692828146, Blast_Score=469, Evalue=1e-133,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 84639; Mature: 84639

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFAEVIVEIKASNTDRLYTYKVPEGMDLEVGMRVLVPFKNRLVEGYVVSLTDKPSYSLEK
CCCCEEEEEECCCCCEEEEEECCCCCCHHCCCEEEEEHHHHCCCCEEEEEECCCCHHHHH
IKSVYKILDKYPIFNEKMIKLAKWMRDYYKCYFSEVFQTIMPAGIDRGEKKVKLVRLKIE
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEHHH
DVFEEDFKKAPRQYEVLRYLKKRSPIKLSELVKILNTDYGTIRALEKKGYIEVYEEEISR
HHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHEEECCCCCCHHHHHHHHHH
FQVRDVEKTFPPILTEEQKRAVEEVKKSIFSGQPDAYLLFGVTGSGKTEVYLKLIEETVK
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHH
IGKSAIVLVPEIALTPQTIERFVSRFGNRVAVIHSGLSPGERFDEWRKVKNKEVDVVVGV
CCCCEEEEECCCCCCHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEEE
RSAVFAPFDNLGLIIIDEEHEATYKQSDTRPRYNAKEVAEKRCEIEKAVLVMASATPSLE
HHHHHCCCCCCCEEEEECCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHEEEEECCCCCHH
TYYKAIKGDYKLLKLTRRIKASMPEIEVVNMSDELAKGNNSIFSRKLFSYIKENLKKGEQ
HHHHHHHCCHHHHHHHHHHHCCCCCEEEECCCHHHHCCCCHHHHHHHHHHHHHHHCCCCE
TILFLNRRGYSSFVACRDCGYVPKCPNCDISLTYHFEDKKLVCHYCGYEEPMRETCPKCG
EEEEEECCCCCHHHHHHCCCCCCCCCCCCEEEEEEECCCEEEEEECCCCCHHHHHHHHHC
SKRIRYMGIGTERVEMDIKKFFPEARVLRMDVDTTRRKGSHEKIFYEFKNGKADILIGTQ
CCCEEEEECCHHHHHHHHHHHCCCCEEEEEECHHHHCCCCCCEEEEEECCCCEEEEEEHH
MISKGFDIPNVTLVGVILADITLNLPDFRSSERTFQLLTQVAGRAGRGEKPGRVVIQTYE
HHHCCCCCCCHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEE
DNHYSILTAKEQDYVKFFREEIKYREIFKYPPFAHLMNIVVSGEDEEKVKNASANTYLAC
CCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHCCCCCHHHHH
QKVINKLQVKSYNKILGPAPAPISKINNRYRWQVIVKSEEREVLEEIAEYVQNMKYPKDI
HHHHHHHHHHHHHHHCCCCCCCHHHCCCCEEEEEEEECHHHHHHHHHHHHHHHCCCCCCE
RVAVDIDPLNIM
EEEEECCCCCCC
>Mature Secondary Structure
MFAEVIVEIKASNTDRLYTYKVPEGMDLEVGMRVLVPFKNRLVEGYVVSLTDKPSYSLEK
CCCCEEEEEECCCCCEEEEEECCCCCCHHCCCEEEEEHHHHCCCCEEEEEECCCCHHHHH
IKSVYKILDKYPIFNEKMIKLAKWMRDYYKCYFSEVFQTIMPAGIDRGEKKVKLVRLKIE
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEHHH
DVFEEDFKKAPRQYEVLRYLKKRSPIKLSELVKILNTDYGTIRALEKKGYIEVYEEEISR
HHHHHHHHHCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCHHEEECCCCCCHHHHHHHHHH
FQVRDVEKTFPPILTEEQKRAVEEVKKSIFSGQPDAYLLFGVTGSGKTEVYLKLIEETVK
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHHHHHHH
IGKSAIVLVPEIALTPQTIERFVSRFGNRVAVIHSGLSPGERFDEWRKVKNKEVDVVVGV
CCCCEEEEECCCCCCHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHCCCCEEEEEEE
RSAVFAPFDNLGLIIIDEEHEATYKQSDTRPRYNAKEVAEKRCEIEKAVLVMASATPSLE
HHHHHCCCCCCCEEEEECCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHEEEEECCCCCHH
TYYKAIKGDYKLLKLTRRIKASMPEIEVVNMSDELAKGNNSIFSRKLFSYIKENLKKGEQ
HHHHHHHCCHHHHHHHHHHHCCCCCEEEECCCHHHHCCCCHHHHHHHHHHHHHHHCCCCE
TILFLNRRGYSSFVACRDCGYVPKCPNCDISLTYHFEDKKLVCHYCGYEEPMRETCPKCG
EEEEEECCCCCHHHHHHCCCCCCCCCCCCEEEEEEECCCEEEEEECCCCCHHHHHHHHHC
SKRIRYMGIGTERVEMDIKKFFPEARVLRMDVDTTRRKGSHEKIFYEFKNGKADILIGTQ
CCCEEEEECCHHHHHHHHHHHCCCCEEEEEECHHHHCCCCCCEEEEEECCCCEEEEEEHH
MISKGFDIPNVTLVGVILADITLNLPDFRSSERTFQLLTQVAGRAGRGEKPGRVVIQTYE
HHHCCCCCCCHHHHHHHHHHHEECCCCCCCCHHHHHHHHHHHCCCCCCCCCCEEEEEEEE
DNHYSILTAKEQDYVKFFREEIKYREIFKYPPFAHLMNIVVSGEDEEKVKNASANTYLAC
CCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCHHHHHCCCCCHHHHH
QKVINKLQVKSYNKILGPAPAPISKINNRYRWQVIVKSEEREVLEEIAEYVQNMKYPKDI
HHHHHHHHHHHHHHHCCCCCCCHHHCCCCEEEEEEEECHHHHHHHHHHHHHHHCCCCCCE
RVAVDIDPLNIM
EEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9534248; 9384377; 9086272 [H]