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The map label for this gene is rlmN

Identifier: 20807945

GI number: 20807945

Start: 1467758

End: 1468786

Strand: Reverse

Name: rlmN

Synonym: TTE1502

Alternate gene names: 20807945

Gene position: 1468786-1467758 (Counterclockwise)

Preceding gene: 20807946

Following gene: 20807944

Centisome position: 54.61

GC content: 36.15

Gene sequence:

>1029_bases
TTGTACAACCTGAAGGATATGACTTTGGAGGAGATGGAAGAGTTTTTTGTCAACTTAGGTGAGAGCAAATTTAGGGCAAA
ACAACTTTACAAGTGGATTTACGACAAAAGGGTCACAGATTTTGATTTGATGACAGACATTTCCAAGAATTTGAGGGCAA
AACTTAAAGAAATTGCCTATATTTCAGAGTTAAAGATAATAGAAAGAAGAGTCTCGCAAATAGACGATACAGTAAAATAC
TTATTTTTGCTTGAAGATAAAAACATAATAGAAGGAGTTGCCATAAAATATAAGTTTGGAAATACAGCTTGTGTATCCAC
ACAGGTTGGCTGTAATATGAAGTGCAAGTTTTGTGCCTCTGCAATAGGAGGAAAAGTCAGAGACCTTAAAGCTTCAGAAA
TGGTAGATCAGGTAATGGCAATAGATAGCGACTATGGCAAAATCTCTAATATAGTCCTTATGGGGAGTGGAGAGCCTTTT
GACAATTACGACGAGGTTATGAAGTTCATAAAAATAGTGAACAATCCTTATGGATTAAAAATTGGGAAAAGGCATATCAC
TATTTCCACAGTAGGAATTGTCCCCAAAATTTATCAATTTGCTGATGAAGAGCTTCAAGTGAATCTCTCTATATCACTAC
ATGCTCCCAACAACGAGTTGAGGAATGAGCTTATGCCAATAAACAGAGCATATCCCTTAGAAGAGCTAATGAAAGCCTGC
AGATATTATATAGAAAAGACTAACAGAAGAATTACTTTTGAATATGCTTTAATTGACGGGGTAAATGACAAAAAGGAACA
CGCTTATCAGCTGGTGGACCTCTTAAAAGGTATGCTCTGTCATGTAAACTTAATACCAATAAATTACGTTAAGGAGATAG
GTTTTAGAAAATCCAACAATGAAAAAGTGATGATGTTTAAGAAAATTATTGAAAACGCCGGCATAACCTGTACTGTGAGA
AGAGAGCTGGGAAGCGATATTGAGGCTGCTTGCGGGCAGTTGAGAAGAAAGTATTTGAAAGAGGGGTAA

Upstream 100 bases:

>100_bases
CTGATTTAGGATATATACAGACGACTCCCTTAGAACACAAGATTGATGGGTTTTTCATAGCTAAATTAAAAAGAATATAT
TAAACTGGAGGTAATAGAAG

Downstream 100 bases:

>100_bases
AGTTTTATGATTGTAGCTGCCCTTACTGATATAGGCAATTTTAGGGAGAAAAATGAAGATTACTATTACGCTTCTTTTGA
CGAAAACTTTCCTCTGTTTA

Product: ribosomal RNA large subunit methyltransferase N

Products: NA

Alternate protein names: 23S rRNA m2A2503 methyltransferase

Number of amino acids: Translated: 342; Mature: 342

Protein sequence:

>342_residues
MYNLKDMTLEEMEEFFVNLGESKFRAKQLYKWIYDKRVTDFDLMTDISKNLRAKLKEIAYISELKIIERRVSQIDDTVKY
LFLLEDKNIIEGVAIKYKFGNTACVSTQVGCNMKCKFCASAIGGKVRDLKASEMVDQVMAIDSDYGKISNIVLMGSGEPF
DNYDEVMKFIKIVNNPYGLKIGKRHITISTVGIVPKIYQFADEELQVNLSISLHAPNNELRNELMPINRAYPLEELMKAC
RYYIEKTNRRITFEYALIDGVNDKKEHAYQLVDLLKGMLCHVNLIPINYVKEIGFRKSNNEKVMMFKKIIENAGITCTVR
RELGSDIEAACGQLRRKYLKEG

Sequences:

>Translated_342_residues
MYNLKDMTLEEMEEFFVNLGESKFRAKQLYKWIYDKRVTDFDLMTDISKNLRAKLKEIAYISELKIIERRVSQIDDTVKY
LFLLEDKNIIEGVAIKYKFGNTACVSTQVGCNMKCKFCASAIGGKVRDLKASEMVDQVMAIDSDYGKISNIVLMGSGEPF
DNYDEVMKFIKIVNNPYGLKIGKRHITISTVGIVPKIYQFADEELQVNLSISLHAPNNELRNELMPINRAYPLEELMKAC
RYYIEKTNRRITFEYALIDGVNDKKEHAYQLVDLLKGMLCHVNLIPINYVKEIGFRKSNNEKVMMFKKIIENAGITCTVR
RELGSDIEAACGQLRRKYLKEG
>Mature_342_residues
MYNLKDMTLEEMEEFFVNLGESKFRAKQLYKWIYDKRVTDFDLMTDISKNLRAKLKEIAYISELKIIERRVSQIDDTVKY
LFLLEDKNIIEGVAIKYKFGNTACVSTQVGCNMKCKFCASAIGGKVRDLKASEMVDQVMAIDSDYGKISNIVLMGSGEPF
DNYDEVMKFIKIVNNPYGLKIGKRHITISTVGIVPKIYQFADEELQVNLSISLHAPNNELRNELMPINRAYPLEELMKAC
RYYIEKTNRRITFEYALIDGVNDKKEHAYQLVDLLKGMLCHVNLIPINYVKEIGFRKSNNEKVMMFKKIIENAGITCTVR
RELGSDIEAACGQLRRKYLKEG

Specific function: Specifically methylates position 2 of adenine 2503 in 23S rRNA

COG id: COG0820

COG function: function code R; Predicted Fe-S-cluster redox enzyme

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. RlmN family

Homologues:

Organism=Escherichia coli, GI1788865, Length=343, Percent_Identity=42.2740524781341, Blast_Score=276, Evalue=2e-75,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMN_THETN (Q8R9T4)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_623116.1
- ProteinModelPortal:   Q8R9T4
- GeneID:   995686
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE1502
- NMPDR:   fig|273068.3.peg.1463
- HOGENOM:   HBG335345
- OMA:   TFCSTAQ
- ProtClustDB:   PRK14454
- BioCyc:   TTEN273068:TTE1502-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01849
- InterPro:   IPR006638
- InterPro:   IPR007197
- InterPro:   IPR004383
- PIRSF:   PIRSF006004
- SMART:   SM00729
- TIGRFAMs:   TIGR00048

Pfam domain/function: PF04055 Radical_SAM

EC number: =2.1.1.192

Molecular weight: Translated: 39454; Mature: 39454

Theoretical pI: Translated: 8.50; Mature: 8.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
6.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYNLKDMTLEEMEEFFVNLGESKFRAKQLYKWIYDKRVTDFDLMTDISKNLRAKLKEIAY
CCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
ISELKIIERRVSQIDDTVKYLFLLEDKNIIEGVAIKYKFGNTACVSTQVGCNMKCKFCAS
HHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCEEEEEECCCCEEEEECCCCCCCHHHHHH
AIGGKVRDLKASEMVDQVMAIDSDYGKISNIVLMGSGEPFDNYDEVMKFIKIVNNPYGLK
HHCCHHHCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCE
IGKRHITISTVGIVPKIYQFADEELQVNLSISLHAPNNELRNELMPINRAYPLEELMKAC
ECCEEEEEEEHHHHHHHHHHCCCCEEEEEEEEEECCCHHHHHHHCCCCCCCCHHHHHHHH
RYYIEKTNRRITFEYALIDGVNDKKEHAYQLVDLLKGMLCHVNLIPINYVKEIGFRKSNN
HHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHEEEEEEHHHHHHHCCCCCCC
EKVMMFKKIIENAGITCTVRRELGSDIEAACGQLRRKYLKEG
CHHHHHHHHHHCCCCEEEEHHHHCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MYNLKDMTLEEMEEFFVNLGESKFRAKQLYKWIYDKRVTDFDLMTDISKNLRAKLKEIAY
CCCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
ISELKIIERRVSQIDDTVKYLFLLEDKNIIEGVAIKYKFGNTACVSTQVGCNMKCKFCAS
HHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCEEEEEECCCCEEEEECCCCCCCHHHHHH
AIGGKVRDLKASEMVDQVMAIDSDYGKISNIVLMGSGEPFDNYDEVMKFIKIVNNPYGLK
HHCCHHHCCHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCCHHHHHHHHHHHCCCCCCE
IGKRHITISTVGIVPKIYQFADEELQVNLSISLHAPNNELRNELMPINRAYPLEELMKAC
ECCEEEEEEEHHHHHHHHHHCCCCEEEEEEEEEECCCHHHHHHHCCCCCCCCHHHHHHHH
RYYIEKTNRRITFEYALIDGVNDKKEHAYQLVDLLKGMLCHVNLIPINYVKEIGFRKSNN
HHHHHHCCCEEEEEEEEECCCCCHHHHHHHHHHHHHHHHHEEEEEEHHHHHHHCCCCCCC
EKVMMFKKIIENAGITCTVRRELGSDIEAACGQLRRKYLKEG
CHHHHHHHHHHCCCCEEEEHHHHCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336