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The map label for this gene is trmD

Identifier: 20807905

GI number: 20807905

Start: 1432107

End: 1432832

Strand: Reverse

Name: trmD

Synonym: TTE1458

Alternate gene names: 20807905

Gene position: 1432832-1432107 (Counterclockwise)

Preceding gene: 20807906

Following gene: 20807904

Centisome position: 53.28

GC content: 39.53

Gene sequence:

>726_bases
ATGATTTTTGATGTTTTAACGCTGTTTCCTGAGATGTTTGAATGCGTTCTTTCTCACAGCATTTTGAAAAGGGCTGTTGA
AAAAAGTAAGCTAAAAGTGAATTTAATCAACATAAGGGATTTTTCAAAAGATAAACATAAAAGAGTAGATGACTATCCTT
ATGGCGGGGGACCGGGGATGGTCATGATGCCCCAGCCCCTTTTTGATGCCATCGAATTTGTGAAAAGTCAGGGGAACATT
CCTGTCTACTACTTAAGTCCAAAGGGCAAAATTTTTACCCAGGAAATGGCAAGAGAACTTTCAAAGATGGAAAGAATTGC
TCTTTTATGCGGCCATTACGAGGGAATTGATGAAAGAGTATATGCAGTGATAGACGGTGAAATCTCAATAGGAGATTTTA
TACTGACAGGTGGAGAATTGGCCGCAATGGTGGTCATAGATGCAGTGACAAGGCTAATTGATGGGGTTTTGTCTCATCCT
CAGAGCGCTTTAGAGGAATCCTTTTCTGACTATCTTCTCGAATACCCTCAGTACACGAGACCAGAAGTATTCAGAGGAAT
GAGAGTGCCTGAGGTGCTTTTAAGTGGCAATCACGCTGAAATTGCCAGATGGAGAAGGCAGAAGTCTTTGGAGATAACCC
TTTTAAAAAGACCTGATTTATTGAAAAAAGCCAATTTGACTGAGGAAGATAAAAAATTTCTTAGAGAAAAAGGATATGAG
ATATAG

Upstream 100 bases:

>100_bases
AAAAGAAAGAGAAGTGCTGTTGCCTGCAATAAAAGAAGTGATAAAGAAAGTTGATGTAGAAGGCAAAGTGATGATTGTGC
GCCTCTTGGAGGGGCTATAA

Downstream 100 bases:

>100_bases
AAAAGGAAGGAGGGAATGTAGCATGGACTTGATAAAGGCTGTGGAAAGCGAGCAGCTTAAGAAAGACATTACGCCTTTTA
ATGTAGGTGACACTGTAAGA

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MIFDVLTLFPEMFECVLSHSILKRAVEKSKLKVNLINIRDFSKDKHKRVDDYPYGGGPGMVMMPQPLFDAIEFVKSQGNI
PVYYLSPKGKIFTQEMARELSKMERIALLCGHYEGIDERVYAVIDGEISIGDFILTGGELAAMVVIDAVTRLIDGVLSHP
QSALEESFSDYLLEYPQYTRPEVFRGMRVPEVLLSGNHAEIARWRRQKSLEITLLKRPDLLKKANLTEEDKKFLREKGYE
I

Sequences:

>Translated_241_residues
MIFDVLTLFPEMFECVLSHSILKRAVEKSKLKVNLINIRDFSKDKHKRVDDYPYGGGPGMVMMPQPLFDAIEFVKSQGNI
PVYYLSPKGKIFTQEMARELSKMERIALLCGHYEGIDERVYAVIDGEISIGDFILTGGELAAMVVIDAVTRLIDGVLSHP
QSALEESFSDYLLEYPQYTRPEVFRGMRVPEVLLSGNHAEIARWRRQKSLEITLLKRPDLLKKANLTEEDKKFLREKGYE
I
>Mature_241_residues
MIFDVLTLFPEMFECVLSHSILKRAVEKSKLKVNLINIRDFSKDKHKRVDDYPYGGGPGMVMMPQPLFDAIEFVKSQGNI
PVYYLSPKGKIFTQEMARELSKMERIALLCGHYEGIDERVYAVIDGEISIGDFILTGGELAAMVVIDAVTRLIDGVLSHP
QSALEESFSDYLLEYPQYTRPEVFRGMRVPEVLLSGNHAEIARWRRQKSLEITLLKRPDLLKKANLTEEDKKFLREKGYE
I

Specific function: Specifically methylates guanosine-37 in various tRNAs

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family

Homologues:

Organism=Escherichia coli, GI1788959, Length=239, Percent_Identity=51.8828451882845, Blast_Score=239, Evalue=1e-64,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRMD_THETN (Q8R5S2)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_623076.1
- ProteinModelPortal:   Q8R5S2
- SMR:   Q8R5S2
- GeneID:   997894
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE1458
- NMPDR:   fig|273068.3.peg.1423
- HOGENOM:   HBG285805
- OMA:   VCGRFEG
- ProtClustDB:   PRK00026
- BioCyc:   TTEN273068:TTE1458-MONOMER
- BRENDA:   2.1.1.31
- GO:   GO:0005737
- HAMAP:   MF_00605
- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148
- Gene3D:   G3DSA:1.10.1270.20
- PIRSF:   PIRSF000386
- TIGRFAMs:   TIGR00088

Pfam domain/function: PF01746 tRNA_m1G_MT

EC number: =2.1.1.31

Molecular weight: Translated: 27605; Mature: 27605

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: NA

Important sites: BINDING 111-111

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFDVLTLFPEMFECVLSHSILKRAVEKSKLKVNLINIRDFSKDKHKRVDDYPYGGGPGM
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCHHHCCCCCCCCCCCCCE
VMMPQPLFDAIEFVKSQGNIPVYYLSPKGKIFTQEMARELSKMERIALLCGHYEGIDERV
EECCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
YAVIDGEISIGDFILTGGELAAMVVIDAVTRLIDGVLSHPQSALEESFSDYLLEYPQYTR
EEEECCCEEECCEEEECCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCC
PEVFRGMRVPEVLLSGNHAEIARWRRQKSLEITLLKRPDLLKKANLTEEDKKFLREKGYE
HHHHHCCCCCHHHHCCCHHHHHHHHHHCCCEEEEECCCCHHHHCCCCHHHHHHHHHCCCC
I
C
>Mature Secondary Structure
MIFDVLTLFPEMFECVLSHSILKRAVEKSKLKVNLINIRDFSKDKHKRVDDYPYGGGPGM
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCCCHHHCCCCCCCCCCCCCE
VMMPQPLFDAIEFVKSQGNIPVYYLSPKGKIFTQEMARELSKMERIALLCGHYEGIDERV
EECCCHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCEE
YAVIDGEISIGDFILTGGELAAMVVIDAVTRLIDGVLSHPQSALEESFSDYLLEYPQYTR
EEEECCCEEECCEEEECCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCC
PEVFRGMRVPEVLLSGNHAEIARWRRQKSLEITLLKRPDLLKKANLTEEDKKFLREKGYE
HHHHHCCCCCHHHHCCCHHHHHHHHHHCCCEEEEECCCCHHHHCCCCHHHHHHHHHCCCC
I
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336