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The map label for this gene is hslU

Identifier: 20807894

GI number: 20807894

Start: 1421008

End: 1422393

Strand: Reverse

Name: hslU

Synonym: TTE1447

Alternate gene names: 20807894

Gene position: 1422393-1421008 (Counterclockwise)

Preceding gene: 20807895

Following gene: 20807893

Centisome position: 52.89

GC content: 39.11

Gene sequence:

>1386_bases
GTGAGGAATGTGAAAAATTACACGCCTAAAGAAATTGTAGCAGAACTTGACAAATACATAGTCGGACAAAAGGAAGCAAA
AAAGTCTGTGGCAGTTGCTTTGAGGAATAGATACAGAAGAAGCCTTTTGCCGGAAGATTTTAAAGAGGAAGTCACCCCCA
AAAACATCTTGATGGTAGGGCCTACTGGAGTGGGTAAGACGGAAATTGCGAGGAGAATAGCTAAACTGGTAGAAGCCCCT
TTTGTAAAAGTAGAAGCTACCAAATTCACTGAAGTAGGATATGTAGGAAGAGATGTGGATTCAATGGTAAGAGACCTGGT
AGAAGCTGCTGTAAGAATGGTAAAAGAAGAAAAATTAAAAGCTGTGACAGAAAAGGCTAAAAAACTAGCTGAAGAAAGAC
TTATAGATTACCTCTTGGGGAAAAAGAAAAAGCAGCCTAAAAATCCTTTTGAAATGATTTTTAACTATCCTTCTAGTGAG
AGGACTGAAGAACCGGATAATGTAGACAGCTACAAAAGGGAAGAAATAAGGCAGAGGCTGAGAAGTGGCGAACTTGACAA
CCACATGGTGGAAATTGAAGTAACTGACACAACTCCCCCAATGCTGGAAATGTACACAAATTTAGGGGCAGAGGAGCTTA
ACATAACACTGCAAGACATGTTTTCTGACCTCTTGCCCAAAAAGAAAAAATTGAGGAAGGTGACAGTAGCTGAAGCGAAA
AAAATATTAGAAGCAGAGGAAGCGCAAAACCTCATTGATATGGATGAAGTGATAGAAGAAGCAGTAAAAAGAGCAGAAAA
TGATGGAATAATATTTATAGATGAAATTGATAAAATTGCCAGTACAGGGTATTCAGCAGGCCCCGATGTATCAAGAGAAG
GAGTTCAAAGAGACCTTCTACCGATAATAGAAGGCTGCACTGTCATGACAAAATACGGGCCTGTGAAAACAGACTACATA
CTGTTCATAGCTGCTGGGGCTTTTAACGTAGCAAAAGTGTCTGACTTAATACCCGAACTTCAAGGAAGATTCCCTATAAG
AGTAAACTTAAAGCCTCTGACTAAAGAAGATTTTGTCAGGATATTAAAAGAACCAAAGAATGCTCTTACAAAGCAGTATC
AGGAGCTTTTGAGAACCGAAGGTGTGGAAATTAAGTATACAGAAGAAGCGATCGAGACAATTGCAGAAGTAGCTTACCTG
ATAAATCAGCAGTCTGAGGATATTGGAGCGAGAAGGCTCCACACTGTAATGGAGAAGTTATTTGAAGACCTTTCTTTCCA
TGCTCCCGAGTTGAGCGGTCAAGAGATAGTAATAACAGCAGATTATGTCAAAGAACAATTAAAAGACAGCTTAAACAAGT
ACGAAGTCAATAAATACATTTTGTAA

Upstream 100 bases:

>100_bases
AGATTTAGATACAGAAGAAATTGCGAGAAAAGCTTTGGAAATAGCTTCAAAGATATGCGTGTATACAAATAACAATATTA
CAGTAGAGACACTCTAGGGG

Downstream 100 bases:

>100_bases
AAAAATAAAAAACACAGGAGGATTGAAAAATGACGCTTTTAGAAAAAACGAGAAAATTAAATCGAATCTTACAAAAGACT
GGCATACAACCGGTAGACTT

Product: ATP-dependent protease ATP-binding subunit HslU

Products: NA

Alternate protein names: Unfoldase HslU

Number of amino acids: Translated: 461; Mature: 461

Protein sequence:

>461_residues
MRNVKNYTPKEIVAELDKYIVGQKEAKKSVAVALRNRYRRSLLPEDFKEEVTPKNILMVGPTGVGKTEIARRIAKLVEAP
FVKVEATKFTEVGYVGRDVDSMVRDLVEAAVRMVKEEKLKAVTEKAKKLAEERLIDYLLGKKKKQPKNPFEMIFNYPSSE
RTEEPDNVDSYKREEIRQRLRSGELDNHMVEIEVTDTTPPMLEMYTNLGAEELNITLQDMFSDLLPKKKKLRKVTVAEAK
KILEAEEAQNLIDMDEVIEEAVKRAENDGIIFIDEIDKIASTGYSAGPDVSREGVQRDLLPIIEGCTVMTKYGPVKTDYI
LFIAAGAFNVAKVSDLIPELQGRFPIRVNLKPLTKEDFVRILKEPKNALTKQYQELLRTEGVEIKYTEEAIETIAEVAYL
INQQSEDIGARRLHTVMEKLFEDLSFHAPELSGQEIVITADYVKEQLKDSLNKYEVNKYIL

Sequences:

>Translated_461_residues
MRNVKNYTPKEIVAELDKYIVGQKEAKKSVAVALRNRYRRSLLPEDFKEEVTPKNILMVGPTGVGKTEIARRIAKLVEAP
FVKVEATKFTEVGYVGRDVDSMVRDLVEAAVRMVKEEKLKAVTEKAKKLAEERLIDYLLGKKKKQPKNPFEMIFNYPSSE
RTEEPDNVDSYKREEIRQRLRSGELDNHMVEIEVTDTTPPMLEMYTNLGAEELNITLQDMFSDLLPKKKKLRKVTVAEAK
KILEAEEAQNLIDMDEVIEEAVKRAENDGIIFIDEIDKIASTGYSAGPDVSREGVQRDLLPIIEGCTVMTKYGPVKTDYI
LFIAAGAFNVAKVSDLIPELQGRFPIRVNLKPLTKEDFVRILKEPKNALTKQYQELLRTEGVEIKYTEEAIETIAEVAYL
INQQSEDIGARRLHTVMEKLFEDLSFHAPELSGQEIVITADYVKEQLKDSLNKYEVNKYIL
>Mature_461_residues
MRNVKNYTPKEIVAELDKYIVGQKEAKKSVAVALRNRYRRSLLPEDFKEEVTPKNILMVGPTGVGKTEIARRIAKLVEAP
FVKVEATKFTEVGYVGRDVDSMVRDLVEAAVRMVKEEKLKAVTEKAKKLAEERLIDYLLGKKKKQPKNPFEMIFNYPSSE
RTEEPDNVDSYKREEIRQRLRSGELDNHMVEIEVTDTTPPMLEMYTNLGAEELNITLQDMFSDLLPKKKKLRKVTVAEAK
KILEAEEAQNLIDMDEVIEEAVKRAENDGIIFIDEIDKIASTGYSAGPDVSREGVQRDLLPIIEGCTVMTKYGPVKTDYI
LFIAAGAFNVAKVSDLIPELQGRFPIRVNLKPLTKEDFVRILKEPKNALTKQYQELLRTEGVEIKYTEEAIETIAEVAYL
INQQSEDIGARRLHTVMEKLFEDLSFHAPELSGQEIVITADYVKEQLKDSLNKYEVNKYIL

Specific function: ATPase subunit of a proteasome-like degradation complex; this subunit has chaperone activity. The binding of ATP and its subsequent hydrolysis by HslU are essential for unfolding of protein substrates subsequently hydrolyzed by HslV. HslU recognizes the N

COG id: COG1220

COG function: function code O; ATP-dependent protease HslVU (ClpYQ), ATPase subunit

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ClpX chaperone family. HslU subfamily

Homologues:

Organism=Homo sapiens, GI7242140, Length=94, Percent_Identity=39.3617021276596, Blast_Score=69, Evalue=1e-11,
Organism=Escherichia coli, GI1790366, Length=454, Percent_Identity=53.5242290748899, Blast_Score=487, Evalue=1e-139,
Organism=Escherichia coli, GI1786642, Length=269, Percent_Identity=31.9702602230483, Blast_Score=105, Evalue=6e-24,
Organism=Saccharomyces cerevisiae, GI6319704, Length=94, Percent_Identity=41.4893617021277, Blast_Score=78, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24648289, Length=249, Percent_Identity=31.7269076305221, Blast_Score=99, Evalue=6e-21,
Organism=Drosophila melanogaster, GI24648291, Length=249, Percent_Identity=31.7269076305221, Blast_Score=99, Evalue=7e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): HSLU_THETN (Q8R9Y3)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_623065.1
- ProteinModelPortal:   Q8R9Y3
- SMR:   Q8R9Y3
- GeneID:   997287
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE1447
- NMPDR:   fig|273068.3.peg.1412
- HOGENOM:   HBG745965
- OMA:   EASKFTE
- ProtClustDB:   PRK05201
- BioCyc:   TTEN273068:TTE1447-MONOMER
- HAMAP:   MF_00249
- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR019489
- InterPro:   IPR004491
- PANTHER:   PTHR11262:SF3
- SMART:   SM00382
- TIGRFAMs:   TIGR00390

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF10431 ClpB_D2-small

EC number: NA

Molecular weight: Translated: 52624; Mature: 52624

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: BINDING 21-21 BINDING 274-274 BINDING 339-339 BINDING 411-411

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRNVKNYTPKEIVAELDKYIVGQKEAKKSVAVALRNRYRRSLLPEDFKEEVTPKNILMVG
CCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEEC
PTGVGKTEIARRIAKLVEAPFVKVEATKFTEVGYVGRDVDSMVRDLVEAAVRMVKEEKLK
CCCCCHHHHHHHHHHHHHCCCEEEECCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AVTEKAKKLAEERLIDYLLGKKKKQPKNPFEMIFNYPSSERTEEPDNVDSYKREEIRQRL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHH
RSGELDNHMVEIEVTDTTPPMLEMYTNLGAEELNITLQDMFSDLLPKKKKLRKVTVAEAK
HCCCCCCEEEEEEECCCCCHHHHHHHCCCHHHHHEEHHHHHHHHCCHHHHHHHHHHHHHH
KILEAEEAQNLIDMDEVIEEAVKRAENDGIIFIDEIDKIASTGYSAGPDVSREGVQRDLL
HHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEECHHHHHHCCCCCCCCCCHHHHHHHHH
PIIEGCTVMTKYGPVKTDYILFIAAGAFNVAKVSDLIPELQGRFPIRVNLKPLTKEDFVR
HHHHCCCCEECCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHH
ILKEPKNALTKQYQELLRTEGVEIKYTEEAIETIAEVAYLINQQSEDIGARRLHTVMEKL
HHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
FEDLSFHAPELSGQEIVITADYVKEQLKDSLNKYEVNKYIL
HHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MRNVKNYTPKEIVAELDKYIVGQKEAKKSVAVALRNRYRRSLLPEDFKEEVTPKNILMVG
CCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCCEEEEC
PTGVGKTEIARRIAKLVEAPFVKVEATKFTEVGYVGRDVDSMVRDLVEAAVRMVKEEKLK
CCCCCHHHHHHHHHHHHHCCCEEEECCHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHH
AVTEKAKKLAEERLIDYLLGKKKKQPKNPFEMIFNYPSSERTEEPDNVDSYKREEIRQRL
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHH
RSGELDNHMVEIEVTDTTPPMLEMYTNLGAEELNITLQDMFSDLLPKKKKLRKVTVAEAK
HCCCCCCEEEEEEECCCCCHHHHHHHCCCHHHHHEEHHHHHHHHCCHHHHHHHHHHHHHH
KILEAEEAQNLIDMDEVIEEAVKRAENDGIIFIDEIDKIASTGYSAGPDVSREGVQRDLL
HHHHHHHHHHHCCHHHHHHHHHHHCCCCCEEEEECHHHHHHCCCCCCCCCCHHHHHHHHH
PIIEGCTVMTKYGPVKTDYILFIAAGAFNVAKVSDLIPELQGRFPIRVNLKPLTKEDFVR
HHHHCCCCEECCCCCCCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEEECCCCCHHHHHH
ILKEPKNALTKQYQELLRTEGVEIKYTEEAIETIAEVAYLINQQSEDIGARRLHTVMEKL
HHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
FEDLSFHAPELSGQEIVITADYVKEQLKDSLNKYEVNKYIL
HHHHHCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336