Click here to switch to the map view.

The map label for this gene is RsuA2 [H]

Identifier: 20807779

GI number: 20807779

Start: 1317706

End: 1318416

Strand: Direct

Name: RsuA2 [H]

Synonym: TTE1332

Alternate gene names: 20807779

Gene position: 1317706-1318416 (Clockwise)

Preceding gene: 20807778

Following gene: 20807780

Centisome position: 49.0

GC content: 36.99

Gene sequence:

>711_bases
ATGGTAAGATTGCAAAAATACCTAGCAGAATGTGGTATAGCTTCAAGGAGAAAATGTGAGGAGTATATCTTACAAGGACG
GGTAAAAGTAAATGGAAAAGTTGTGAAAGAATTAGGTACAAAAATAGACCCGGATGTTGACATAATCGAATTTGATGATA
AAATTGTGAGAAGAGAAGAAAAGAAAGTGTATATAATGCTCTACAAGCCAGCAGGATATATCACTTCTGTAAAGGACCCT
TTTGGCAGACCTACTGTTTTGGACCTAGTGAAGGTAAAAGAAAGGATATATCCTGTTGGCCGTCTTGACTTTGATACTTC
TGGTTTGCTTCTTCTTACAAACGACGGAGAATTGGCAAATATATTGATGCACCCAAAACACGAAATAGTCAAGACTTATG
TGGCCAAGATTAAAGGGATTCCTACAAAAGAAGAAATGGAAAGGTTTGAAAATGGGTTGATTATTGACGGAAGGAAAACG
GCAAAAGCTAAAATACGCATTTTGAATGTAAAAAATGGGACAAGCGTGGTTGAGATACAGATCCACGAAGGGAGAAACAG
ACAAGTGAAAAAGATGTGTAAAGCGATTGGACATCCTGTCATAGCTCTTAAAAGGACAAAGATAGGTGAACTAGAATTAA
AAGGTTTAAAGCCGGGAGAATGGCGTTATTTAACAGAAGATGAGATAAGATATTTAAAAAGCCTGAGGTGA

Upstream 100 bases:

>100_bases
CACCACTGACACGGAAGCAATAAAGAAGAAAGTTATGAAAGATAACTTGTTAGGAATTTACGAAGAAAAATTGGTGTAAA
ATAAAATAGGGTGACAACAA

Downstream 100 bases:

>100_bases
ATTATGTTTCATTTGAGATTTGCCAAAAGAGAAGACTTTCCCAAAATTGAAAAATTGGCAAAAGAATGTGGGGTAACTTC
CGATTTGGATTTTTTGAGTG

Product: 16S rRNA uridine-516 pseudouridylate synthase family protein

Products: NA

Alternate protein names: rRNA pseudouridylate synthase B; rRNA-uridine isomerase B [H]

Number of amino acids: Translated: 236; Mature: 236

Protein sequence:

>236_residues
MVRLQKYLAECGIASRRKCEEYILQGRVKVNGKVVKELGTKIDPDVDIIEFDDKIVRREEKKVYIMLYKPAGYITSVKDP
FGRPTVLDLVKVKERIYPVGRLDFDTSGLLLLTNDGELANILMHPKHEIVKTYVAKIKGIPTKEEMERFENGLIIDGRKT
AKAKIRILNVKNGTSVVEIQIHEGRNRQVKKMCKAIGHPVIALKRTKIGELELKGLKPGEWRYLTEDEIRYLKSLR

Sequences:

>Translated_236_residues
MVRLQKYLAECGIASRRKCEEYILQGRVKVNGKVVKELGTKIDPDVDIIEFDDKIVRREEKKVYIMLYKPAGYITSVKDP
FGRPTVLDLVKVKERIYPVGRLDFDTSGLLLLTNDGELANILMHPKHEIVKTYVAKIKGIPTKEEMERFENGLIIDGRKT
AKAKIRILNVKNGTSVVEIQIHEGRNRQVKKMCKAIGHPVIALKRTKIGELELKGLKPGEWRYLTEDEIRYLKSLR
>Mature_236_residues
MVRLQKYLAECGIASRRKCEEYILQGRVKVNGKVVKELGTKIDPDVDIIEFDDKIVRREEKKVYIMLYKPAGYITSVKDP
FGRPTVLDLVKVKERIYPVGRLDFDTSGLLLLTNDGELANILMHPKHEIVKTYVAKIKGIPTKEEMERFENGLIIDGRKT
AKAKIRILNVKNGTSVVEIQIHEGRNRQVKKMCKAIGHPVIALKRTKIGELELKGLKPGEWRYLTEDEIRYLKSLR

Specific function: Responsible for synthesis of pseudouridine from uracil- 2633 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=230, Percent_Identity=36.5217391304348, Blast_Score=134, Evalue=4e-33,
Organism=Escherichia coli, GI1788510, Length=231, Percent_Identity=35.9307359307359, Blast_Score=131, Evalue=4e-32,
Organism=Escherichia coli, GI1787524, Length=241, Percent_Identity=36.5145228215768, Blast_Score=130, Evalue=7e-32,
Organism=Escherichia coli, GI87081838, Length=177, Percent_Identity=32.7683615819209, Blast_Score=102, Evalue=2e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 27112; Mature: 27112

Theoretical pI: Translated: 10.20; Mature: 10.20

Prosite motif: PS50889 S4 ; PS01149 PSI_RSU

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVRLQKYLAECGIASRRKCEEYILQGRVKVNGKVVKELGTKIDPDVDIIEFDDKIVRREE
CCHHHHHHHHCCCCCHHHHHHHHHCCEEEECCHHHHHCCCCCCCCCEEEECCHHHHHCCC
KKVYIMLYKPAGYITSVKDPFGRPTVLDLVKVKERIYPVGRLDFDTSGLLLLTNDGELAN
CEEEEEEECCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEE
ILMHPKHEIVKTYVAKIKGIPTKEEMERFENGLIIDGRKTAKAKIRILNVKNGTSVVEIQ
EEECCHHHHHHHHHHHHCCCCCHHHHHHHHCCEEEECCCCCEEEEEEEEECCCCEEEEEE
IHEGRNRQVKKMCKAIGHPVIALKRTKIGELELKGLKPGEWRYLTEDEIRYLKSLR
EECCCCHHHHHHHHHHCCCEEEEECCCCCCEEECCCCCCCEEEECHHHHHHHHHCC
>Mature Secondary Structure
MVRLQKYLAECGIASRRKCEEYILQGRVKVNGKVVKELGTKIDPDVDIIEFDDKIVRREE
CCHHHHHHHHCCCCCHHHHHHHHHCCEEEECCHHHHHCCCCCCCCCEEEECCHHHHHCCC
KKVYIMLYKPAGYITSVKDPFGRPTVLDLVKVKERIYPVGRLDFDTSGLLLLTNDGELAN
CEEEEEEECCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCEEE
ILMHPKHEIVKTYVAKIKGIPTKEEMERFENGLIIDGRKTAKAKIRILNVKNGTSVVEIQ
EEECCHHHHHHHHHHHHCCCCCHHHHHHHHCCEEEECCCCCEEEEEEEEECCCCEEEEEE
IHEGRNRQVKKMCKAIGHPVIALKRTKIGELELKGLKPGEWRYLTEDEIRYLKSLR
EECCCCHHHHHHHHHHCCCEEEEECCCCCCEEECCCCCCCEEEECHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7934829; 9384377; 9888802 [H]