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The map label for this gene is ppnK

Identifier: 20807749

GI number: 20807749

Start: 1291051

End: 1291902

Strand: Direct

Name: ppnK

Synonym: TTE1300

Alternate gene names: 20807749

Gene position: 1291051-1291902 (Clockwise)

Preceding gene: 20807748

Following gene: 20807750

Centisome position: 48.0

GC content: 36.97

Gene sequence:

>852_bases
ATGAAAAAAGTCGGGGTAATTCCAAATATAAATAAAGACAAGGACTTAGAGGTTACAAAATCTGTAGTAAATTGGCTTTT
AGATCATGGTTCTGAACCCTATTTAAATGAGATAGTGGCAGCCAGAATCGGCTATGAAAAACACGGCAAAAAAGCAAATG
AAATATACAGCAAATCTGACTTTCTCATCGCTTTAGGAGGAGATGGAACGATACTTAATGTAGCGCGCCTTTGTGCTCCT
TTTGGCACCCCAATTCTAGCAGTAAATTTGGGACATTTAGGCTTTTTGACAGAAATAGATGCATCTGAACTTTTCCCTTC
ACTAGAGAAAATATACAAAGGGGAGTACGCAATAGAAAAGAGAATGATGTTAGAAGCTAATGTGGTTAAAAATGACATGG
AAGTTATAAATTTCAGGGCTTTGAATGACATAGTTATAACAAGAGGAGCTTTTTCAAGAATGGCCCGCATTAAAGCTTAT
GTAAATGATAATTACGTAGATACTTACTTAGCAGATGGGGTTATTGTAGCAACACCTACTGGCTCTACAGCCTATTCTCT
TTCTGCTGGAGGGCCAATCGTATATCCCACAGTGGAAGTCATCATCATAACGCCAATTTGTCCACACACTTTGTACTCCA
GGTCTATAGTTGTCTCTCCTGATGATGTAATACGCCTTGAGATAGCTGAAGAAAACCAGGATTTAATGATAACTACTGAT
GGACAGCAAGGCTACAAGATAGATTATAGAGATGTAATATACATAAAGAAAAGCAATGAATACACTAATCTCATCAAAGT
GAAAAACAGCAATTTCTTTGATTTGCTAAGAGATAAGCTTACAGAAAGGTGA

Upstream 100 bases:

>100_bases
AGGAAATAGAAGTTGAAAAAATAGTTGAAGAAGCCTTTAAAAATTTGTGGCAGGAAAAATGAATTTTGTGTAGAATAATG
ATAAATGTGGTGATATAATA

Downstream 100 bases:

>100_bases
AAAGAGGTGAAAGGAAGAATGATGAAGTTGGCAAGGCATGCCAAGATTTTAGAGATAATTTCTGAAAAAGAAATAGAGAC
GCAGGAAGAGCTGGCAGCAG

Product: kinase

Products: NA

Alternate protein names: Poly(P)/ATP NAD kinase

Number of amino acids: Translated: 283; Mature: 283

Protein sequence:

>283_residues
MKKVGVIPNINKDKDLEVTKSVVNWLLDHGSEPYLNEIVAARIGYEKHGKKANEIYSKSDFLIALGGDGTILNVARLCAP
FGTPILAVNLGHLGFLTEIDASELFPSLEKIYKGEYAIEKRMMLEANVVKNDMEVINFRALNDIVITRGAFSRMARIKAY
VNDNYVDTYLADGVIVATPTGSTAYSLSAGGPIVYPTVEVIIITPICPHTLYSRSIVVSPDDVIRLEIAEENQDLMITTD
GQQGYKIDYRDVIYIKKSNEYTNLIKVKNSNFFDLLRDKLTER

Sequences:

>Translated_283_residues
MKKVGVIPNINKDKDLEVTKSVVNWLLDHGSEPYLNEIVAARIGYEKHGKKANEIYSKSDFLIALGGDGTILNVARLCAP
FGTPILAVNLGHLGFLTEIDASELFPSLEKIYKGEYAIEKRMMLEANVVKNDMEVINFRALNDIVITRGAFSRMARIKAY
VNDNYVDTYLADGVIVATPTGSTAYSLSAGGPIVYPTVEVIIITPICPHTLYSRSIVVSPDDVIRLEIAEENQDLMITTD
GQQGYKIDYRDVIYIKKSNEYTNLIKVKNSNFFDLLRDKLTER
>Mature_283_residues
MKKVGVIPNINKDKDLEVTKSVVNWLLDHGSEPYLNEIVAARIGYEKHGKKANEIYSKSDFLIALGGDGTILNVARLCAP
FGTPILAVNLGHLGFLTEIDASELFPSLEKIYKGEYAIEKRMMLEANVVKNDMEVINFRALNDIVITRGAFSRMARIKAY
VNDNYVDTYLADGVIVATPTGSTAYSLSAGGPIVYPTVEVIIITPICPHTLYSRSIVVSPDDVIRLEIAEENQDLMITTD
GQQGYKIDYRDVIYIKKSNEYTNLIKVKNSNFFDLLRDKLTER

Specific function: Catalyzes the phosphorylation of NAD to NADP. Utilizes ATP and other nucleoside triphosphates as well as inorganic polyphosphate as a source of phosphorus

COG id: COG0061

COG function: function code G; Predicted sugar kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the NAD kinase family

Homologues:

Organism=Homo sapiens, GI55743112, Length=257, Percent_Identity=32.6848249027237, Blast_Score=112, Evalue=3e-25,
Organism=Escherichia coli, GI1788968, Length=282, Percent_Identity=32.9787234042553, Blast_Score=174, Evalue=7e-45,
Organism=Saccharomyces cerevisiae, GI6325068, Length=214, Percent_Identity=35.981308411215, Blast_Score=129, Evalue=7e-31,
Organism=Saccharomyces cerevisiae, GI6320794, Length=312, Percent_Identity=30.7692307692308, Blast_Score=126, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6322509, Length=215, Percent_Identity=31.1627906976744, Blast_Score=112, Evalue=7e-26,
Organism=Drosophila melanogaster, GI28573828, Length=249, Percent_Identity=29.718875502008, Blast_Score=101, Evalue=6e-22,
Organism=Drosophila melanogaster, GI28573830, Length=249, Percent_Identity=29.718875502008, Blast_Score=100, Evalue=9e-22,
Organism=Drosophila melanogaster, GI161077047, Length=249, Percent_Identity=29.718875502008, Blast_Score=100, Evalue=9e-22,
Organism=Drosophila melanogaster, GI28573826, Length=249, Percent_Identity=29.718875502008, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI28573832, Length=249, Percent_Identity=29.718875502008, Blast_Score=100, Evalue=1e-21,
Organism=Drosophila melanogaster, GI20129957, Length=274, Percent_Identity=26.2773722627737, Blast_Score=93, Evalue=2e-19,
Organism=Drosophila melanogaster, GI281363321, Length=274, Percent_Identity=26.2773722627737, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI281363323, Length=274, Percent_Identity=26.2773722627737, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24653422, Length=274, Percent_Identity=26.2773722627737, Blast_Score=92, Evalue=3e-19,
Organism=Drosophila melanogaster, GI24653424, Length=274, Percent_Identity=26.2773722627737, Blast_Score=92, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PPNK_THETN (Q8RAC3)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_622920.1
- ProteinModelPortal:   Q8RAC3
- SMR:   Q8RAC3
- GeneID:   996870
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE1300
- NMPDR:   fig|273068.3.peg.1191
- HOGENOM:   HBG713904
- OMA:   FESFMID
- ProtClustDB:   CLSK901156
- BioCyc:   TTEN273068:TTE1300-MONOMER
- BRENDA:   2.7.1.23
- GO:   GO:0005737
- HAMAP:   MF_00361
- InterPro:   IPR016064
- InterPro:   IPR017438
- InterPro:   IPR017437
- InterPro:   IPR002504
- Gene3D:   G3DSA:2.60.200.30
- Gene3D:   G3DSA:3.40.50.10330
- PANTHER:   PTHR20275

Pfam domain/function: PF01513 NAD_kinase; SSF111331 ATP-NAD_kinase_PpnK-typ

EC number: =2.7.1.23

Molecular weight: Translated: 31618; Mature: 31618

Theoretical pI: Translated: 5.44; Mature: 5.44

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKVGVIPNINKDKDLEVTKSVVNWLLDHGSEPYLNEIVAARIGYEKHGKKANEIYSKSD
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHCCCHHHHHHCCCC
FLIALGGDGTILNVARLCAPFGTPILAVNLGHLGFLTEIDASELFPSLEKIYKGEYAIEK
EEEEECCCCCHHHHHHHHHCCCCCEEEEECCCCEEEEECCHHHHHHHHHHHHCCCHHHHH
RMMLEANVVKNDMEVINFRALNDIVITRGAFSRMARIKAYVNDNYVDTYLADGVIVATPT
HHHHHHHHHHCCHHEEEEEEECCEEEECHHHHHHHHHHHHHCCCEEEHHHHCCEEEEECC
GSTAYSLSAGGPIVYPTVEVIIITPICPHTLYSRSIVVSPDDVIRLEIAEENQDLMITTD
CCCEEEECCCCCEEECCEEEEEECCCCCCHHHCCEEEECCCCEEEEEEECCCCCEEEEEC
GQQGYKIDYRDVIYIKKSNEYTNLIKVKNSNFFDLLRDKLTER
CCCCCEECCEEEEEEEECCCCEEEEEEECCCHHHHHHHHHCCC
>Mature Secondary Structure
MKKVGVIPNINKDKDLEVTKSVVNWLLDHGSEPYLNEIVAARIGYEKHGKKANEIYSKSD
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHCCCHHHHHHCCCC
FLIALGGDGTILNVARLCAPFGTPILAVNLGHLGFLTEIDASELFPSLEKIYKGEYAIEK
EEEEECCCCCHHHHHHHHHCCCCCEEEEECCCCEEEEECCHHHHHHHHHHHHCCCHHHHH
RMMLEANVVKNDMEVINFRALNDIVITRGAFSRMARIKAYVNDNYVDTYLADGVIVATPT
HHHHHHHHHHCCHHEEEEEEECCEEEECHHHHHHHHHHHHHCCCEEEHHHHCCEEEEECC
GSTAYSLSAGGPIVYPTVEVIIITPICPHTLYSRSIVVSPDDVIRLEIAEENQDLMITTD
CCCEEEECCCCCEEECCEEEEEECCCCCCHHHCCEEEECCCCEEEEEEECCCCCEEEEEC
GQQGYKIDYRDVIYIKKSNEYTNLIKVKNSNFFDLLRDKLTER
CCCCCEECCEEEEEEEECCCCEEEEEEECCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336