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The map label for this gene is ProB

Identifier: 20807726

GI number: 20807726

Start: 1272895

End: 1274013

Strand: Direct

Name: ProB

Synonym: TTE1277

Alternate gene names: 20807726

Gene position: 1272895-1274013 (Clockwise)

Preceding gene: 20807725

Following gene: 20807727

Centisome position: 47.33

GC content: 40.93

Gene sequence:

>1119_bases
ATGAGGATTGTAGTTAAGGTTGGGACTAGTACTTTGACTCATGAAAATGGGAAATTGAATTTAGAAATAATGGAAAAACT
AGTAAGACAAATCGCAAATTTGTCAAATAAAGGAGAAGAAGTTATACTTGTGACGTCTGGGGCGATTGGAGCTGGAATGG
GAAAATTAAACTTGACAGAAAAGCCCAAAAACCTTCCGCAAAAGCAGGCCCTAGCAGCCATTGGTCAAGGTCTCCTTATA
GAGATTTACGAGAAATTTTTCAACGAATACGGGAAAATAACTGCCCAGCTTCTTTTGACGAGGGAGGACTTTAGTGACAG
AAAAAGGTATTTAAATATGAGCTACACCCTCTCTAACCTTCTCAAGTGGGGAGTCATACCTGTGATAAATGAAAATGACA
CTGTGGCAGTAGAGGAGATAAAGATTGGGGATAATGATACCCTGGCAGCCCTTGTGGCAAGCCTTGTTGAAGCAGACCTT
TTGATTATTTTGACTGATATCGATGGCCTTTTTGACAAGGACCCGAGAATTTATAAGGATGCTAAGGTTATAGAAGTGGT
TGAGGAGTTTTCTGATGACCTTTTTAAAATAGCTGGCGGGGCAGGTACAAAAAGAGGAACAGGAGGAATGTACACAAAGA
TTCAGGCAGCCAAGATATGCTACAATTCGGGAGTTAAAATGGTGATTGCAAATGGCAAAATTGACAATGTATTAAATAGA
ATTGCAGCAGGAGAAAAAATTGGGACTACTTTTCTTCCCATGAAAAATCCTATAAGCAGCAGAAAAATCTGGATTGCTTT
CAATGCAAAGGTGAATGGTTTTTTGTTTGTAGATGAAGGGGCAGCAAAGGCTTTAGTAAAGCATGGAAAAAGCTTACTGC
CAAGCGGTATTGTAAAGACAGAAGGCCAGTATGACGTTGGGGACTGCGTGGCAGTGGTGGACCACCAGGGCAGGGAAATA
GCTAGAGGGCTTGTCAACTATTCTTCTGAGGAAGTCGAAAAAATAAAAGGCTGTAAGACCCATGACATAGAGTCAATATT
GGGGTATAAGTACTACGACGAGGTTATTCACAGGGACAATCTCGTAGTTCTTGAGAGAGGTGAGAAGTATGGAGGTTGA

Upstream 100 bases:

>100_bases
TGGAGCCCATAGTTATTTGTGAATCTCGTGAGAGGATGGCAGAAGACGCATTAGAACTTAAGAGAATATACCAAGAGGTG
GCAAAAAGGGTGTCAAAAAC

Downstream 100 bases:

>100_bases
AGTAAAGGCTAAAAAGGCGAAAGAAGCGTCAAGAAAAATGGCTGTACTTGACACAGAAACTAAAAATAGAGCTCTTATAA
ATATGGCAGAAGCTCTTTTG

Product: gamma-glutamyl kinase

Products: NA

Alternate protein names: Gamma-glutamyl kinase; GK

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MRIVVKVGTSTLTHENGKLNLEIMEKLVRQIANLSNKGEEVILVTSGAIGAGMGKLNLTEKPKNLPQKQALAAIGQGLLI
EIYEKFFNEYGKITAQLLLTREDFSDRKRYLNMSYTLSNLLKWGVIPVINENDTVAVEEIKIGDNDTLAALVASLVEADL
LIILTDIDGLFDKDPRIYKDAKVIEVVEEFSDDLFKIAGGAGTKRGTGGMYTKIQAAKICYNSGVKMVIANGKIDNVLNR
IAAGEKIGTTFLPMKNPISSRKIWIAFNAKVNGFLFVDEGAAKALVKHGKSLLPSGIVKTEGQYDVGDCVAVVDHQGREI
ARGLVNYSSEEVEKIKGCKTHDIESILGYKYYDEVIHRDNLVVLERGEKYGG

Sequences:

>Translated_372_residues
MRIVVKVGTSTLTHENGKLNLEIMEKLVRQIANLSNKGEEVILVTSGAIGAGMGKLNLTEKPKNLPQKQALAAIGQGLLI
EIYEKFFNEYGKITAQLLLTREDFSDRKRYLNMSYTLSNLLKWGVIPVINENDTVAVEEIKIGDNDTLAALVASLVEADL
LIILTDIDGLFDKDPRIYKDAKVIEVVEEFSDDLFKIAGGAGTKRGTGGMYTKIQAAKICYNSGVKMVIANGKIDNVLNR
IAAGEKIGTTFLPMKNPISSRKIWIAFNAKVNGFLFVDEGAAKALVKHGKSLLPSGIVKTEGQYDVGDCVAVVDHQGREI
ARGLVNYSSEEVEKIKGCKTHDIESILGYKYYDEVIHRDNLVVLERGEKYGG
>Mature_372_residues
MRIVVKVGTSTLTHENGKLNLEIMEKLVRQIANLSNKGEEVILVTSGAIGAGMGKLNLTEKPKNLPQKQALAAIGQGLLI
EIYEKFFNEYGKITAQLLLTREDFSDRKRYLNMSYTLSNLLKWGVIPVINENDTVAVEEIKIGDNDTLAALVASLVEADL
LIILTDIDGLFDKDPRIYKDAKVIEVVEEFSDDLFKIAGGAGTKRGTGGMYTKIQAAKICYNSGVKMVIANGKIDNVLNR
IAAGEKIGTTFLPMKNPISSRKIWIAFNAKVNGFLFVDEGAAKALVKHGKSLLPSGIVKTEGQYDVGDCVAVVDHQGREI
ARGLVNYSSEEVEKIKGCKTHDIESILGYKYYDEVIHRDNLVVLERGEKYGG

Specific function: Catalyzes the transfer of a phosphate group to glutamate to form glutamate 5-phosphate which rapidly cyclizes to 5- oxoproline

COG id: COG0263

COG function: function code E; Glutamate 5-kinase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PUA domain

Homologues:

Organism=Homo sapiens, GI21361368, Length=289, Percent_Identity=34.6020761245675, Blast_Score=150, Evalue=2e-36,
Organism=Homo sapiens, GI62912457, Length=287, Percent_Identity=34.4947735191638, Blast_Score=149, Evalue=4e-36,
Organism=Escherichia coli, GI1786437, Length=360, Percent_Identity=43.3333333333333, Blast_Score=295, Evalue=3e-81,
Organism=Caenorhabditis elegans, GI17569969, Length=271, Percent_Identity=35.4243542435424, Blast_Score=150, Evalue=1e-36,
Organism=Caenorhabditis elegans, GI17569967, Length=271, Percent_Identity=35.4243542435424, Blast_Score=150, Evalue=1e-36,
Organism=Saccharomyces cerevisiae, GI6320506, Length=417, Percent_Identity=35.0119904076739, Blast_Score=236, Evalue=4e-63,
Organism=Saccharomyces cerevisiae, GI6321822, Length=413, Percent_Identity=34.8668280871671, Blast_Score=230, Evalue=4e-61,
Organism=Drosophila melanogaster, GI21357643, Length=283, Percent_Identity=36.3957597173145, Blast_Score=157, Evalue=1e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): PROB_THETN (Q8RAE6)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_622897.1
- ProteinModelPortal:   Q8RAE6
- SMR:   Q8RAE6
- GeneID:   997603
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE1277
- NMPDR:   fig|273068.3.peg.1115
- HOGENOM:   HBG507643
- OMA:   TFGDNDM
- ProtClustDB:   PRK05429
- BioCyc:   TTEN273068:TTE1277-MONOMER
- BRENDA:   2.7.2.11
- GO:   GO:0005737
- HAMAP:   MF_00456
- InterPro:   IPR001048
- InterPro:   IPR001057
- InterPro:   IPR011529
- InterPro:   IPR005715
- InterPro:   IPR019797
- InterPro:   IPR002478
- InterPro:   IPR015947
- Gene3D:   G3DSA:3.40.1160.10
- PIRSF:   PIRSF000729
- PRINTS:   PR00474
- SMART:   SM00359
- TIGRFAMs:   TIGR01027

Pfam domain/function: PF00696 AA_kinase; PF01472 PUA; SSF53633 Aa_kinase; SSF88697 PUA-like

EC number: =2.7.2.11

Molecular weight: Translated: 40841; Mature: 40841

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: PS00902 GLUTAMATE_5_KINASE; PS50890 PUA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRIVVKVGTSTLTHENGKLNLEIMEKLVRQIANLSNKGEEVILVTSGAIGAGMGKLNLTE
CEEEEEECCCEEECCCCEEEHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCC
KPKNLPQKQALAAIGQGLLIEIYEKFFNEYGKITAQLLLTREDFSDRKRYLNMSYTLSNL
CCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHEEHHHEEECCCHHHHHHHEEHHHHHHHH
LKWGVIPVINENDTVAVEEIKIGDNDTLAALVASLVEADLLIILTDIDGLFDKDPRIYKD
HHHCCEEEECCCCEEEEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHH
AKVIEVVEEFSDDLFKIAGGAGTKRGTGGMYTKIQAAKICYNSGVKMVIANGKIDNVLNR
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHCCCCEEEEECCCHHHHHHH
IAAGEKIGTTFLPMKNPISSRKIWIAFNAKVNGFLFVDEGAAKALVKHGKSLLPSGIVKT
HHCCCCCCCEECCCCCCCCCCEEEEEEECCEEEEEEEECCHHHHHHHCCHHHCCCCCEEC
EGQYDVGDCVAVVDHQGREIARGLVNYSSEEVEKIKGCKTHDIESILGYKYYDEVIHRDN
CCCCCCCCEEEEECCCCHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCC
LVVLERGEKYGG
EEEEECCCCCCC
>Mature Secondary Structure
MRIVVKVGTSTLTHENGKLNLEIMEKLVRQIANLSNKGEEVILVTSGAIGAGMGKLNLTE
CEEEEEECCCEEECCCCEEEHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCCCCCCCC
KPKNLPQKQALAAIGQGLLIEIYEKFFNEYGKITAQLLLTREDFSDRKRYLNMSYTLSNL
CCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHEEHHHEEECCCHHHHHHHEEHHHHHHHH
LKWGVIPVINENDTVAVEEIKIGDNDTLAALVASLVEADLLIILTDIDGLFDKDPRIYKD
HHHCCEEEECCCCEEEEEEEEECCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCHHHH
AKVIEVVEEFSDDLFKIAGGAGTKRGTGGMYTKIQAAKICYNSGVKMVIANGKIDNVLNR
HHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEHHHHHHHHCCCCEEEEECCCHHHHHHH
IAAGEKIGTTFLPMKNPISSRKIWIAFNAKVNGFLFVDEGAAKALVKHGKSLLPSGIVKT
HHCCCCCCCEECCCCCCCCCCEEEEEEECCEEEEEEEECCHHHHHHHCCHHHCCCCCEEC
EGQYDVGDCVAVVDHQGREIARGLVNYSSEEVEKIKGCKTHDIESILGYKYYDEVIHRDN
CCCCCCCCEEEEECCCCHHHHHHHHCCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCC
LVVLERGEKYGG
EEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336