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The map label for this gene is AroK

Identifier: 20807724

GI number: 20807724

Start: 1271510

End: 1272025

Strand: Direct

Name: AroK

Synonym: TTE1275

Alternate gene names: 20807724

Gene position: 1271510-1272025 (Clockwise)

Preceding gene: 20807723

Following gene: 20807725

Centisome position: 47.28

GC content: 38.57

Gene sequence:

>516_bases
ATGAAAAACATAGTTTTGACAGGATTCATGGGGACAGGAAAAACTACTGTAGGGAAAAAAGTGGCAACTACTCTTCATTT
TAATTTTATTGACACTGACAAGCTGGTTGAGAAAATGGCAGGCATGAGTGTGGCGGAAATATTTGAAAAGCACGGGGAAG
AATATTTTAGAAAGTTGGAAAAAGCGGCTGTCATAAAAGCCTCTCGCCTAAAAAATCACGTAATAGCTACAGGAGGCGGG
GTTGTATTAAACCCTTCAAATATTGTACAATTAAGAAAAAACGGAGTAATAATCCTCTTAAAAGCAAGGCCGGAAGTAAT
TTTGAGGAATATATCCAAAACAAAAGATAGGCCTCTTCTTGCGGTGGAAGACCCTGAAAAGAGGATAAGAGAGCTTTTAG
AGGAAAGAGAGCCTTTTTACAGATTTGCAGATTACACTATAGACGTCTCTGACAAGACAATAGAGGAAGTCGCTGAAGAA
GTCATAAGAGCGTATATAAAGCTTAAGGGGAGATGA

Upstream 100 bases:

>100_bases
AAAGTCCCCCTCCAGTAGATGTGAACAAAGTAATATCTGATACTTTAGAAAAAATCTTGAAAGAGGCTATACCTTCCAAA
CCATAAAAGGGGGATGGTGA

Downstream 100 bases:

>100_bases
TGAATTTTGGGGATTAAGTTTGGGCCTTCTGGAATCTCGCAGAGCTTTTACGACGAGGGACATAAGTCTTCTGCTGAGAT
GCCAGAATGGCTTCATAAAA

Product: shikimate kinase

Products: NA

Alternate protein names: SK

Number of amino acids: Translated: 171; Mature: 171

Protein sequence:

>171_residues
MKNIVLTGFMGTGKTTVGKKVATTLHFNFIDTDKLVEKMAGMSVAEIFEKHGEEYFRKLEKAAVIKASRLKNHVIATGGG
VVLNPSNIVQLRKNGVIILLKARPEVILRNISKTKDRPLLAVEDPEKRIRELLEEREPFYRFADYTIDVSDKTIEEVAEE
VIRAYIKLKGR

Sequences:

>Translated_171_residues
MKNIVLTGFMGTGKTTVGKKVATTLHFNFIDTDKLVEKMAGMSVAEIFEKHGEEYFRKLEKAAVIKASRLKNHVIATGGG
VVLNPSNIVQLRKNGVIILLKARPEVILRNISKTKDRPLLAVEDPEKRIRELLEEREPFYRFADYTIDVSDKTIEEVAEE
VIRAYIKLKGR
>Mature_171_residues
MKNIVLTGFMGTGKTTVGKKVATTLHFNFIDTDKLVEKMAGMSVAEIFEKHGEEYFRKLEKAAVIKASRLKNHVIATGGG
VVLNPSNIVQLRKNGVIILLKARPEVILRNISKTKDRPLLAVEDPEKRIRELLEEREPFYRFADYTIDVSDKTIEEVAEE
VIRAYIKLKGR

Specific function: Catalyzes the specific phosphorylation of the 3-hydroxyl group of shikimic acid using ATP as a cosubstrate

COG id: COG0703

COG function: function code E; Shikimate kinase

Gene ontology:

Cell location: Cytoplasm (Probable)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate kinase family

Homologues:

Organism=Escherichia coli, GI87082255, Length=163, Percent_Identity=37.4233128834356, Blast_Score=104, Evalue=2e-24,
Organism=Escherichia coli, GI1786587, Length=162, Percent_Identity=33.9506172839506, Blast_Score=90, Evalue=8e-20,

Paralogues:

None

Copy number: 140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 200 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): AROK_THETN (Q8RAE8)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_622895.1
- ProteinModelPortal:   Q8RAE8
- SMR:   Q8RAE8
- GeneID:   997597
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE1275
- NMPDR:   fig|273068.3.peg.1113
- HOGENOM:   HBG335815
- OMA:   VIYLRAN
- ProtClustDB:   PRK13947
- BioCyc:   TTEN273068:TTE1275-MONOMER
- BRENDA:   2.7.1.71
- GO:   GO:0005737
- HAMAP:   MF_00109
- InterPro:   IPR000623
- InterPro:   IPR023000
- PRINTS:   PR01100

Pfam domain/function: PF01202 SKI

EC number: =2.7.1.71

Molecular weight: Translated: 19369; Mature: 19369

Theoretical pI: Translated: 10.19; Mature: 10.19

Prosite motif: PS01128 SHIKIMATE_KINASE

Important sites: BINDING 33-33 BINDING 57-57 BINDING 79-79 BINDING 117-117 BINDING 136-136

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKNIVLTGFMGTGKTTVGKKVATTLHFNFIDTDKLVEKMAGMSVAEIFEKHGEEYFRKLE
CCCEEEEEECCCCCCHHCHHHHHEEEEEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
KAAVIKASRLKNHVIATGGGVVLNPSNIVQLRKNGVIILLKARPEVILRNISKTKDRPLL
HHHHHHHHHHCCCEEEECCCEEECCHHEEEEECCCEEEEEECCHHHHHHHHHCCCCCCEE
AVEDPEKRIRELLEEREPFYRFADYTIDVSDKTIEEVAEEVIRAYIKLKGR
EECCHHHHHHHHHHHCCCCEEHHCEEEECCHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKNIVLTGFMGTGKTTVGKKVATTLHFNFIDTDKLVEKMAGMSVAEIFEKHGEEYFRKLE
CCCEEEEEECCCCCCHHCHHHHHEEEEEECCHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
KAAVIKASRLKNHVIATGGGVVLNPSNIVQLRKNGVIILLKARPEVILRNISKTKDRPLL
HHHHHHHHHHCCCEEEECCCEEECCHHEEEEECCCEEEEEECCHHHHHHHHHCCCCCCEE
AVEDPEKRIRELLEEREPFYRFADYTIDVSDKTIEEVAEEVIRAYIKLKGR
EECCHHHHHHHHHHHCCCCEEHHCEEEECCHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11997336