Click here to switch to the map view.

The map label for this gene is GspE [H]

Identifier: 20807711

GI number: 20807711

Start: 1261755

End: 1263425

Strand: Direct

Name: GspE [H]

Synonym: TTE1262

Alternate gene names: 20807711

Gene position: 1261755-1263425 (Clockwise)

Preceding gene: 20807710

Following gene: 20807712

Centisome position: 46.92

GC content: 41.71

Gene sequence:

>1671_bases
ATGGCTAAGAAAAAACTTGGAGAACTTCTTGTGGAAGTGGGACTTATCACGGAAGAACAACTGAAACATGCCATGGAAGT
GCAGAGCAAAACAGGGGAAAAATTGGGGAAAGTCCTTACAAAGCTCGGGTATGTGACAGAAAATCAGATATTAGAAGCCC
TTGAATTTCAGCTGGGAATACCCCATGTGGACCTTCAGAAATACTACATTGACCCTGAGGTTGCAAAGCTTGTTCCTGAA
TCTGTAGCCAAGAGGCATCTCCTCATCCCTGTTAAAAAAAGTGAAGACGGCCTTTATGTAGCAATGGCAGACCCCCTCAA
TATTTTTGCCTTGGATGATGTGAGAATTATAACTAAACAAAATGTGAAACCTTTGATTGCATCGGAGAGCAGCATTCTAA
AAGCTATAGATAGAATCTATGGCAAAGAACAGGCAGAAAAAGCGGCTCAGGAATTTAAAAAGGAGTTTAAAGAAGAGGAA
AGCCTACCTCAAGAAGTGCTGGAAGAAATCCAAAATGCTCCTGCTGTAAGATTTGTCAATTCTATAATTGAGCAGGCTGT
GAAAAGCAGAGCCTCAGATATACACATTGAGCCGACAGAAAAGGATTTGAGAATTCGCTTCAGAATAGATGGGCAGTTGA
CAGAAGCAATGAGGACTATAAAAAGCACGCATGCTCCTGTTGTGACTAGAATAAAGATAATGGCCAATATGAATATCGCC
GAAAGAAGGCTTCCACAAGACGGGAGATTTGATTTCACGGTGTCAGGCAGAACCGTAGATGTAAGGGTTTCCTCCCTTCC
TACTGTGTTTGGCGAAAAGATAGTTTTAAGGCTTCTTGACAAGGAAAACTTTGTGATGACAAAAGAACAACTTGGATTTG
ATGAGGAGGATTTGCCTCTTTTTGATAAACTGATAAAAAGACCCAATGGAATTGTGCTTTTAACAGGTCCTACGGGAAGT
GGTAAAACTACTACTCTTTATGCAATGCTAAGAGAGCTCAATAAGCCCAATGTGAACATAATAACAGTAGAAGACCCTGT
TGAGTATTCGCTGGAAGGCATAAATCAAGTGCAGGTAAATGAAAAAGCAGGGCTTACATTTGCTACTGCACTAAGGTCCA
TCTTAAGGCAGGACCCTGATATAATAATGATTGGGGAGATAAGAGATACAGAGACAGCTGAGATCGCCATAAGAGCGGCG
ATAACAGGTCATTTAGTGCTTTCGACGCTGCACACCAACAACGCTGCAGGTGCGATAACGAGACTGATAGATATGGGGAT
TGAGCCTTATTTGGTGGCTTCTTCTGTAGTGGGAGTGATAGCTCAAAGGCTTGCTAGAAAGGTGTGCGACAATTGCAAAG
TTCCCTATAAGGCTTCAAAGGAAGAAAAAGAGATTTTGGGGATTCCGGAAGATGAAGAGGTCACTCTTTATAAAGGCAAA
GGCTGTCCTGTCTGCAACAGGACAGGCTACAGAGGGCGTATCCCTATATACGAGATAATGACTGTGACTTCTGACATAAG
AGAACTCATAAATGCAAAGGTTTCCTCAGATGTGATTGAAGAAAAAGCTATAAAAAATGGCATGAAGACTTTGAGAGAAA
GCGCAAGAAAGCTCATTTTACAGGGCAAAACTACTGTCGAAGAGATGGTGAGACTAACTTATGAGGAATGA

Upstream 100 bases:

>100_bases
AGGCTAGCTATGCTTTTTACCTCTGGACAGGAGAGTTTTTTGACAAAGACTTTGTGTATGAGAAAATAAGGGGAGAAATG
TAGAGGGAAGAGGTATTTTT

Downstream 100 bases:

>100_bases
TGAGGATGGAGACCATTGAACTTTTGAAATTGGTTGTGGAGAAAAAAGCTTCAGATTTACACATAACAGTAGGAGTTCCA
CCTGTTGTAAGAATTAACGG

Product: pili biogenesis protein PilB-like ATPase

Products: NA

Alternate protein names: Type II traffic warden ATPase [H]

Number of amino acids: Translated: 556; Mature: 555

Protein sequence:

>556_residues
MAKKKLGELLVEVGLITEEQLKHAMEVQSKTGEKLGKVLTKLGYVTENQILEALEFQLGIPHVDLQKYYIDPEVAKLVPE
SVAKRHLLIPVKKSEDGLYVAMADPLNIFALDDVRIITKQNVKPLIASESSILKAIDRIYGKEQAEKAAQEFKKEFKEEE
SLPQEVLEEIQNAPAVRFVNSIIEQAVKSRASDIHIEPTEKDLRIRFRIDGQLTEAMRTIKSTHAPVVTRIKIMANMNIA
ERRLPQDGRFDFTVSGRTVDVRVSSLPTVFGEKIVLRLLDKENFVMTKEQLGFDEEDLPLFDKLIKRPNGIVLLTGPTGS
GKTTTLYAMLRELNKPNVNIITVEDPVEYSLEGINQVQVNEKAGLTFATALRSILRQDPDIIMIGEIRDTETAEIAIRAA
ITGHLVLSTLHTNNAAGAITRLIDMGIEPYLVASSVVGVIAQRLARKVCDNCKVPYKASKEEKEILGIPEDEEVTLYKGK
GCPVCNRTGYRGRIPIYEIMTVTSDIRELINAKVSSDVIEEKAIKNGMKTLRESARKLILQGKTTVEEMVRLTYEE

Sequences:

>Translated_556_residues
MAKKKLGELLVEVGLITEEQLKHAMEVQSKTGEKLGKVLTKLGYVTENQILEALEFQLGIPHVDLQKYYIDPEVAKLVPE
SVAKRHLLIPVKKSEDGLYVAMADPLNIFALDDVRIITKQNVKPLIASESSILKAIDRIYGKEQAEKAAQEFKKEFKEEE
SLPQEVLEEIQNAPAVRFVNSIIEQAVKSRASDIHIEPTEKDLRIRFRIDGQLTEAMRTIKSTHAPVVTRIKIMANMNIA
ERRLPQDGRFDFTVSGRTVDVRVSSLPTVFGEKIVLRLLDKENFVMTKEQLGFDEEDLPLFDKLIKRPNGIVLLTGPTGS
GKTTTLYAMLRELNKPNVNIITVEDPVEYSLEGINQVQVNEKAGLTFATALRSILRQDPDIIMIGEIRDTETAEIAIRAA
ITGHLVLSTLHTNNAAGAITRLIDMGIEPYLVASSVVGVIAQRLARKVCDNCKVPYKASKEEKEILGIPEDEEVTLYKGK
GCPVCNRTGYRGRIPIYEIMTVTSDIRELINAKVSSDVIEEKAIKNGMKTLRESARKLILQGKTTVEEMVRLTYEE
>Mature_555_residues
AKKKLGELLVEVGLITEEQLKHAMEVQSKTGEKLGKVLTKLGYVTENQILEALEFQLGIPHVDLQKYYIDPEVAKLVPES
VAKRHLLIPVKKSEDGLYVAMADPLNIFALDDVRIITKQNVKPLIASESSILKAIDRIYGKEQAEKAAQEFKKEFKEEES
LPQEVLEEIQNAPAVRFVNSIIEQAVKSRASDIHIEPTEKDLRIRFRIDGQLTEAMRTIKSTHAPVVTRIKIMANMNIAE
RRLPQDGRFDFTVSGRTVDVRVSSLPTVFGEKIVLRLLDKENFVMTKEQLGFDEEDLPLFDKLIKRPNGIVLLTGPTGSG
KTTTLYAMLRELNKPNVNIITVEDPVEYSLEGINQVQVNEKAGLTFATALRSILRQDPDIIMIGEIRDTETAEIAIRAAI
TGHLVLSTLHTNNAAGAITRLIDMGIEPYLVASSVVGVIAQRLARKVCDNCKVPYKASKEEKEILGIPEDEEVTLYKGKG
CPVCNRTGYRGRIPIYEIMTVTSDIRELINAKVSSDVIEEKAIKNGMKTLRESARKLILQGKTTVEEMVRLTYEE

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of a variety of enzymes across the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=397, Percent_Identity=47.3551637279597, Blast_Score=399, Evalue=1e-112,
Organism=Escherichia coli, GI1786296, Length=462, Percent_Identity=39.6103896103896, Blast_Score=339, Evalue=2e-94,
Organism=Escherichia coli, GI87082188, Length=249, Percent_Identity=38.5542168674699, Blast_Score=137, Evalue=2e-33,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 62240; Mature: 62109

Theoretical pI: Translated: 6.24; Mature: 6.24

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKKLGELLVEVGLITEEQLKHAMEVQSKTGEKLGKVLTKLGYVTENQILEALEFQLGI
CCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
PHVDLQKYYIDPEVAKLVPESVAKRHLLIPVKKSEDGLYVAMADPLNIFALDDVRIITKQ
CCCCHHHEECCHHHHHHHHHHHHCCEEEEEEEECCCCEEEEEECCCEEEEECCEEEEECC
NVKPLIASESSILKAIDRIYGKEQAEKAAQEFKKEFKEEESLPQEVLEEIQNAPAVRFVN
CCCCHHCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHH
SIIEQAVKSRASDIHIEPTEKDLRIRFRIDGQLTEAMRTIKSTHAPVVTRIKIMANMNIA
HHHHHHHHCCCCCEEECCCCCCEEEEEEECCHHHHHHHHHHHCCCCHHHEEEEEECCCHH
ERRLPQDGRFDFTVSGRTVDVRVSSLPTVFGEKIVLRLLDKENFVMTKEQLGFDEEDLPL
HHCCCCCCCEEEEECCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEEHHHCCCCCCCCCH
FDKLIKRPNGIVLLTGPTGSGKTTTLYAMLRELNKPNVNIITVEDPVEYSLEGINQVQVN
HHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEEECCCHHHHHCCCCEEEEC
EKAGLTFATALRSILRQDPDIIMIGEIRDTETAEIAIRAAITGHLVLSTLHTNNAAGAIT
CCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHEEEEHHHHHHHHEEEHCCCCHHHHHH
RLIDMGIEPYLVASSVVGVIAQRLARKVCDNCKVPYKASKEEKEILGIPEDEEVTLYKGK
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCEEEEECC
GCPVCNRTGYRGRIPIYEIMTVTSDIRELINAKVSSDVIEEKAIKNGMKTLRESARKLIL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGKTTVEEMVRLTYEE
CCCHHHHHHHHHHCCC
>Mature Secondary Structure 
AKKKLGELLVEVGLITEEQLKHAMEVQSKTGEKLGKVLTKLGYVTENQILEALEFQLGI
CHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCC
PHVDLQKYYIDPEVAKLVPESVAKRHLLIPVKKSEDGLYVAMADPLNIFALDDVRIITKQ
CCCCHHHEECCHHHHHHHHHHHHCCEEEEEEEECCCCEEEEEECCCEEEEECCEEEEECC
NVKPLIASESSILKAIDRIYGKEQAEKAAQEFKKEFKEEESLPQEVLEEIQNAPAVRFVN
CCCCHHCCHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCHHHHHH
SIIEQAVKSRASDIHIEPTEKDLRIRFRIDGQLTEAMRTIKSTHAPVVTRIKIMANMNIA
HHHHHHHHCCCCCEEECCCCCCEEEEEEECCHHHHHHHHHHHCCCCHHHEEEEEECCCHH
ERRLPQDGRFDFTVSGRTVDVRVSSLPTVFGEKIVLRLLDKENFVMTKEQLGFDEEDLPL
HHCCCCCCCEEEEECCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEEHHHCCCCCCCCCH
FDKLIKRPNGIVLLTGPTGSGKTTTLYAMLRELNKPNVNIITVEDPVEYSLEGINQVQVN
HHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHCCCCCEEEEEECCCHHHHHCCCCEEEEC
EKAGLTFATALRSILRQDPDIIMIGEIRDTETAEIAIRAAITGHLVLSTLHTNNAAGAIT
CCCCCHHHHHHHHHHHCCCCEEEEECCCCCCHHHEEEEHHHHHHHHEEEHCCCCHHHHHH
RLIDMGIEPYLVASSVVGVIAQRLARKVCDNCKVPYKASKEEKEILGIPEDEEVTLYKGK
HHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHCCCCCCCCCEEEEECC
GCPVCNRTGYRGRIPIYEIMTVTSDIRELINAKVSSDVIEEKAIKNGMKTLRESARKLIL
CCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QGKTTVEEMVRLTYEE
CCCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1588814; 10984043 [H]