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The map label for this gene is pilT [H]

Identifier: 20807712

GI number: 20807712

Start: 1263431

End: 1264480

Strand: Direct

Name: pilT [H]

Synonym: TTE1263

Alternate gene names: 20807712

Gene position: 1263431-1264480 (Clockwise)

Preceding gene: 20807711

Following gene: 20807713

Centisome position: 46.98

GC content: 41.05

Gene sequence:

>1050_bases
ATGGAGACCATTGAACTTTTGAAATTGGTTGTGGAGAAAAAAGCTTCAGATTTACACATAACAGTAGGAGTTCCACCTGT
TGTAAGAATTAACGGCTATCTTCAAAAATTAGAAGGAGAGCCTTTTACACCTAAAGATACAGAGGAGATAGTCAAAGACC
TACTTACGAGCGAGCAGCTAAAAAAGCTGGAGGAAAATGGGGATATTGACCTTTCCTATTCTGTGACAGGTTTGGGAAGG
TTTAGAATAAATGTGTACAAGCAAAGAGGAAGTTACAGCCTTGCCATAAGGTCTGTTGCCTTGAGAATACCTACTATAGA
AGAGCTGGGACTTCCACTCATACTTAAAGATTTGGCTTTGAAAACGAGGGGATTGATACTTGTTACAGGACCTACGGGAA
GTGGGAAGTCCACCACACTGGCGGCTATGGTGGACTGGATAAATTCTAAAAGGACTTGTCACATTCTTACATTGGAGGAC
CCTATAGAGTATTTGCACAAGCACAATAAAAGCATAGTAAATCAAAGAGAAATTGGGCACGATGCTACATCCTTTGCATC
GGCTTTAAGAGCTGCATTAAGAGAAGACCCGGATGTGATACTTGTCGGTGAGATGAGGGATTTGGAGACAATACAGATTG
CAATAACTGCTGCCGAGACAGGCCATCTTGTTCTTTCCACTCTTCACACAATAGGAGCTGTAAAAACGATTGATAGGATT
ATTGATGTTTTTCCGCCTCATCAGCAACAGCAAATAAGGATACAGCTTTCCAACGTATTAGAAGGAATCATATCTCAGCA
GCTTATTCCTAAAAAAGACGGAAGCGGAAGAGTTGTTGCCACAGAAGTGATGATTGCAACCCCTGCTATAAGGAATCTCA
TAAGGGAAGGGAAGACTTACCAAATACAGTCGGCAGTTCAAACAGGTGGTAAATTTGGAATGATTACTATGGACATGTCT
ATTTTGCACCTTTTAAAATCAGGGGTTATCTCTTTGGAAGATGCTTTAACTTACTGCGTTGACCAGGAGAGCTTTTCACG
CATGATATAG

Upstream 100 bases:

>100_bases
ATGGCATGAAGACTTTGAGAGAAAGCGCAAGAAAGCTCATTTTACAGGGCAAAACTACTGTCGAAGAGATGGTGAGACTA
ACTTATGAGGAATGATGAGG

Downstream 100 bases:

>100_bases
GGGTGATTTTATGCCTTTGTACTTTTACAAAGCAAGGGACATGGGGGGAAACTTAATTACAGGGACTTTGGAGGTAGATT
CAAAGTCCCAGTGCATAGAC

Product: pili biogenesis protein PilT-like ATPase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 349; Mature: 349

Protein sequence:

>349_residues
METIELLKLVVEKKASDLHITVGVPPVVRINGYLQKLEGEPFTPKDTEEIVKDLLTSEQLKKLEENGDIDLSYSVTGLGR
FRINVYKQRGSYSLAIRSVALRIPTIEELGLPLILKDLALKTRGLILVTGPTGSGKSTTLAAMVDWINSKRTCHILTLED
PIEYLHKHNKSIVNQREIGHDATSFASALRAALREDPDVILVGEMRDLETIQIAITAAETGHLVLSTLHTIGAVKTIDRI
IDVFPPHQQQQIRIQLSNVLEGIISQQLIPKKDGSGRVVATEVMIATPAIRNLIREGKTYQIQSAVQTGGKFGMITMDMS
ILHLLKSGVISLEDALTYCVDQESFSRMI

Sequences:

>Translated_349_residues
METIELLKLVVEKKASDLHITVGVPPVVRINGYLQKLEGEPFTPKDTEEIVKDLLTSEQLKKLEENGDIDLSYSVTGLGR
FRINVYKQRGSYSLAIRSVALRIPTIEELGLPLILKDLALKTRGLILVTGPTGSGKSTTLAAMVDWINSKRTCHILTLED
PIEYLHKHNKSIVNQREIGHDATSFASALRAALREDPDVILVGEMRDLETIQIAITAAETGHLVLSTLHTIGAVKTIDRI
IDVFPPHQQQQIRIQLSNVLEGIISQQLIPKKDGSGRVVATEVMIATPAIRNLIREGKTYQIQSAVQTGGKFGMITMDMS
ILHLLKSGVISLEDALTYCVDQESFSRMI
>Mature_349_residues
METIELLKLVVEKKASDLHITVGVPPVVRINGYLQKLEGEPFTPKDTEEIVKDLLTSEQLKKLEENGDIDLSYSVTGLGR
FRINVYKQRGSYSLAIRSVALRIPTIEELGLPLILKDLALKTRGLILVTGPTGSGKSTTLAAMVDWINSKRTCHILTLED
PIEYLHKHNKSIVNQREIGHDATSFASALRAALREDPDVILVGEMRDLETIQIAITAAETGHLVLSTLHTIGAVKTIDRI
IDVFPPHQQQQIRIQLSNVLEGIISQQLIPKKDGSGRVVATEVMIATPAIRNLIREGKTYQIQSAVQTGGKFGMITMDMS
ILHLLKSGVISLEDALTYCVDQESFSRMI

Specific function: May be involved in pilus retraction [H]

COG id: COG2805

COG function: function code NU; Tfp pilus assembly protein, pilus retraction ATPase PilT

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI87082188, Length=328, Percent_Identity=47.8658536585366, Blast_Score=303, Evalue=1e-83,
Organism=Escherichia coli, GI1789723, Length=158, Percent_Identity=48.7341772151899, Blast_Score=122, Evalue=5e-29,
Organism=Escherichia coli, GI1786296, Length=383, Percent_Identity=32.1148825065274, Blast_Score=119, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 38560; Mature: 38560

Theoretical pI: Translated: 6.80; Mature: 6.80

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
METIELLKLVVEKKASDLHITVGVPPVVRINGYLQKLEGEPFTPKDTEEIVKDLLTSEQL
CCHHHHHHHHHHCCCCCEEEEECCCCEEEECHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
KKLEENGDIDLSYSVTGLGRFRINVYKQRGSYSLAIRSVALRIPTIEELGLPLILKDLAL
HHHHCCCCEEEEEEEEEEEEEEEEEEEECCCEEEEEEEHHEECCCHHHCCCCHHHHHHHH
KTRGLILVTGPTGSGKSTTLAAMVDWINSKRTCHILTLEDPIEYLHKHNKSIVNQREIGH
HHCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHCHHHHHHHHCCC
DATSFASALRAALREDPDVILVGEMRDLETIQIAITAAETGHLVLSTLHTIGAVKTIDRI
CHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
IDVFPPHQQQQIRIQLSNVLEGIISQQLIPKKDGSGRVVATEVMIATPAIRNLIREGKTY
HHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEHHCHHHHHHHHCCCCE
QIQSAVQTGGKFGMITMDMSILHLLKSGVISLEDALTYCVDQESFSRMI
EEHHHHHCCCCEEEEEEHHHHHHHHHHCCEEHHHHHHHHHCHHHHHCCC
>Mature Secondary Structure
METIELLKLVVEKKASDLHITVGVPPVVRINGYLQKLEGEPFTPKDTEEIVKDLLTSEQL
CCHHHHHHHHHHCCCCCEEEEECCCCEEEECHHHHHHCCCCCCCCCHHHHHHHHHHHHHH
KKLEENGDIDLSYSVTGLGRFRINVYKQRGSYSLAIRSVALRIPTIEELGLPLILKDLAL
HHHHCCCCEEEEEEEEEEEEEEEEEEEECCCEEEEEEEHHEECCCHHHCCCCHHHHHHHH
KTRGLILVTGPTGSGKSTTLAAMVDWINSKRTCHILTLEDPIEYLHKHNKSIVNQREIGH
HHCCEEEEECCCCCCCCHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHCHHHHHHHHCCC
DATSFASALRAALREDPDVILVGEMRDLETIQIAITAAETGHLVLSTLHTIGAVKTIDRI
CHHHHHHHHHHHHHCCCCEEEEECCCCCEEEEEEEEECCCCHHHHHHHHHHHHHHHHHHH
IDVFPPHQQQQIRIQLSNVLEGIISQQLIPKKDGSGRVVATEVMIATPAIRNLIREGKTY
HHCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEHHCHHHHHHHHCCCCE
QIQSAVQTGGKFGMITMDMSILHLLKSGVISLEDALTYCVDQESFSRMI
EEHHHHHCCCCEEEEEEHHHHHHHHHHCCEEHHHHHHHHHCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]