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The map label for this gene is RpoD [H]

Identifier: 20807708

GI number: 20807708

Start: 1259648

End: 1260343

Strand: Direct

Name: RpoD [H]

Synonym: TTE1259

Alternate gene names: 20807708

Gene position: 1259648-1260343 (Clockwise)

Preceding gene: 20807707

Following gene: 20807709

Centisome position: 46.84

GC content: 38.36

Gene sequence:

>696_bases
GTGTGGGAAGTTATTGCTGCACTTGTAGGAACCATAGTAAAGGAGATACTGAATTTAGGTTACCTTACTAACACCAATTC
TTTTCCAAGCCCTTTATCCCCTGAAGAAGAGAGAAAGTACTTGGAAGCGTACAGAAGAGGAGACGAAGAGGCAAGGAATA
TATTGATTGAGAGAAATTTAAGACTGGTCGCTCATGTGGTAAAAAAGTATAGCGGGACAGGAAGAGATTTAGATGACCTT
ATTTCTATAGGGACAATTGGACTTATTAAAGCCATAGATACTTATGACTTTGAAAAAGGCACACATCTTGCCACATATGC
TGCAAAGTGCATAGAAAATGAAATCCTCATGTCCATAAGGGCAGAGAAAAAGATGAAAGGAGAAGTTTTTCTTCAAGACC
CAATAGGGGTAGATAAGGAAGGAAATGAAATCTCACTAATGGATGTACTTGGGACAGATGAGGATGAGGTATCTGAGCAG
GTGGAAAAGAAGATACAGATTAAAAAGCTTTACAGTAAGTTAAACAGCGTCTTAAAAAACAGGGAGCGCATGATAATAGA
AATGAGATATGGGCTTTACAATGGAAGAGTGAGGACTCAGAGGGAAATTGCGAAAATGCTTGGAATTTCTCGTTCTTACG
TATCGAGAATTGAGAAGAAAGCCTTGAATAAGCTTTATAAAGAGCTTTCAATGTGA

Upstream 100 bases:

>100_bases
AATAGCCGAAGAGATAATTAAATACTGATGTACAGGCACCTTTTTCAGATAAAACCATATAATAAATGTGTAATTTATTA
AAAAAGAAGAGGTGGCGGCC

Downstream 100 bases:

>100_bases
ACTGTCTTATTGACAGTCTATTTTTTTGTAGTATATAATTTTAACTAAATGAGGTGGGCTTATGTATGAAAAACTAATTC
CTGACATGATTGTGGAAAGT

Product: sporulation sigma factor SigK

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 231; Mature: 231

Protein sequence:

>231_residues
MWEVIAALVGTIVKEILNLGYLTNTNSFPSPLSPEEERKYLEAYRRGDEEARNILIERNLRLVAHVVKKYSGTGRDLDDL
ISIGTIGLIKAIDTYDFEKGTHLATYAAKCIENEILMSIRAEKKMKGEVFLQDPIGVDKEGNEISLMDVLGTDEDEVSEQ
VEKKIQIKKLYSKLNSVLKNRERMIIEMRYGLYNGRVRTQREIAKMLGISRSYVSRIEKKALNKLYKELSM

Sequences:

>Translated_231_residues
MWEVIAALVGTIVKEILNLGYLTNTNSFPSPLSPEEERKYLEAYRRGDEEARNILIERNLRLVAHVVKKYSGTGRDLDDL
ISIGTIGLIKAIDTYDFEKGTHLATYAAKCIENEILMSIRAEKKMKGEVFLQDPIGVDKEGNEISLMDVLGTDEDEVSEQ
VEKKIQIKKLYSKLNSVLKNRERMIIEMRYGLYNGRVRTQREIAKMLGISRSYVSRIEKKALNKLYKELSM
>Mature_231_residues
MWEVIAALVGTIVKEILNLGYLTNTNSFPSPLSPEEERKYLEAYRRGDEEARNILIERNLRLVAHVVKKYSGTGRDLDDL
ISIGTIGLIKAIDTYDFEKGTHLATYAAKCIENEILMSIRAEKKMKGEVFLQDPIGVDKEGNEISLMDVLGTDEDEVSEQ
VEKKIQIKKLYSKLNSVLKNRERMIIEMRYGLYNGRVRTQREIAKMLGISRSYVSRIEKKALNKLYKELSM

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor directs the transcription of crystal protein genes, a sporulation-regulated event [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=246, Percent_Identity=24.7967479674797, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1789871, Length=97, Percent_Identity=39.1752577319588, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014209
- InterPro:   IPR016263
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 26482; Mature: 26482

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MWEVIAALVGTIVKEILNLGYLTNTNSFPSPLSPEEERKYLEAYRRGDEEARNILIERNL
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHH
RLVAHVVKKYSGTGRDLDDLISIGTIGLIKAIDTYDFEKGTHLATYAAKCIENEILMSIR
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
AEKKMKGEVFLQDPIGVDKEGNEISLMDVLGTDEDEVSEQVEKKIQIKKLYSKLNSVLKN
HHHHCCCCEEEECCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RERMIIEMRYGLYNGRVRTQREIAKMLGISRSYVSRIEKKALNKLYKELSM
HHHHHHHHHHCHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MWEVIAALVGTIVKEILNLGYLTNTNSFPSPLSPEEERKYLEAYRRGDEEARNILIERNL
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHH
RLVAHVVKKYSGTGRDLDDLISIGTIGLIKAIDTYDFEKGTHLATYAAKCIENEILMSIR
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHH
AEKKMKGEVFLQDPIGVDKEGNEISLMDVLGTDEDEVSEQVEKKIQIKKLYSKLNSVLKN
HHHHCCCCEEEECCCCCCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
RERMIIEMRYGLYNGRVRTQREIAKMLGISRSYVSRIEKKALNKLYKELSM
HHHHHHHHHHCHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12721629 [H]