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The map label for this gene is hisS

Identifier: 20807682

GI number: 20807682

Start: 1231272

End: 1232525

Strand: Direct

Name: hisS

Synonym: TTE1230

Alternate gene names: 20807682

Gene position: 1231272-1232525 (Clockwise)

Preceding gene: 20807681

Following gene: 20807683

Centisome position: 45.78

GC content: 41.63

Gene sequence:

>1254_bases
ATGCTTACAAAAGCTCCAAGAGGTACTAAGGACGTTTTGCCCTCAGAATCCTATAAATGGCAGTATGTGGAGAATCTGAT
GAGGGAAATATGCGAATTTTACGGGTACAAAGAAATACGCACCCCTGGATTTGAGCATACAGAACTTTTCTTAAGAGGGG
TTGGGGAGTCTACTGACATTGTGAGAAAAGAGATGTACACTTTTAATGATAGGTCTGGAAGGAGCATTACTCTAAAAGCA
GAAGGAACTTCTCCTGCAGTTAGAGCTTTTATAGAGCATGGACTTTACGCAGAGACGCAGCCTACAAAGCTTTACTACAT
AACCCCTGTGTACAGGTATGAAAAGCCTCAAGCAGGAAGGTTAAGAGAGCACCATCAGTTTGGGGTGGAGATTTTCGGGG
CAAAAAGCGCTTCTGCTGATGCAGAAGTCATAAGCATTGCCATGACTCTTTTAAAAAAGTTAGGCCTTAATAACCTGGAA
CTTAGAATAAATAGCGTGGGGTGTCCTGTTTGCAGGAAGAATTACAACAAGGTGTTGAAGGAGTTTTTGAAAGAACACCT
GGATGAGCTCTGTGACGATTGTAAGGTGAGATACGAAGTCAATCCTTTAAGAGTGCTTGACTGCAAAGTGGAAAGTTGCA
GAAGGGTTACAGGGGAGGCACCTCTTATTACGGATTATCTTTGTGATGACTGCAGAAACCATTTTGAAGAGCTAAAGAAG
TATCTGGATGCTATGGGATATGATTACATAGTCGATCCCAGAATAGTGAGAGGGCTTGATTATTACACTAAAACCGCTTT
TGAAATAATTTCAAAGGACATTGGCGCTCAGGGCACTGTCTGCGGCGGTGGGAGGTATGATGGGCTCATAGAAGAGTGCG
GCGGACCTTCAATGCCTGGGGTAGGCTTTGGTATGGGAATAGAAAGGCTTCTTCTGACTCTGGAGCAGAATGGCATTGAA
ATTCCTAAGCCAGAGGGGCCAGACCTTTTTATAGCTTACATTGGCGATGAAGCGAAGCTTTTTACTTTTACCCTTGCGAA
TAAACTCAGGTTTAACGGTTTAAAGGTAGAGATAGACCATATGGAAAGAAGTTTAAAGGCGCAAATGAAATATGCCAATA
AATTAAATGCCAAATTTGCTGTGGTGATAGGTGAAGAAGAACTCGAGAGCAAAAAAGTGAAATTGAAGAATATGGCTACA
GGAGAAGAGACAGAAATTTTAATTGATGAAATAGAAAAAGCGATAAAAAATTGA

Upstream 100 bases:

>100_bases
TGAGTACCAATCAGGGTGGCACCACGGGATTAACCTCTCGTCCCTGCGTTCTGCAGACGAGGGGTTTTTAAATGTAAGAA
GATAAAAAGGAGTTGGTGAG

Downstream 100 bases:

>100_bases
ATGGAGGGAGTTTAATGGGAGAACAACTTGGCGGTCTTAAAAGAACCCATATGTGCGGAGAGCTCGGGGTTAAAGATGTG
GGAAAGTCTGTCGTAGTGAT

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS

Number of amino acids: Translated: 417; Mature: 417

Protein sequence:

>417_residues
MLTKAPRGTKDVLPSESYKWQYVENLMREICEFYGYKEIRTPGFEHTELFLRGVGESTDIVRKEMYTFNDRSGRSITLKA
EGTSPAVRAFIEHGLYAETQPTKLYYITPVYRYEKPQAGRLREHHQFGVEIFGAKSASADAEVISIAMTLLKKLGLNNLE
LRINSVGCPVCRKNYNKVLKEFLKEHLDELCDDCKVRYEVNPLRVLDCKVESCRRVTGEAPLITDYLCDDCRNHFEELKK
YLDAMGYDYIVDPRIVRGLDYYTKTAFEIISKDIGAQGTVCGGGRYDGLIEECGGPSMPGVGFGMGIERLLLTLEQNGIE
IPKPEGPDLFIAYIGDEAKLFTFTLANKLRFNGLKVEIDHMERSLKAQMKYANKLNAKFAVVIGEEELESKKVKLKNMAT
GEETEILIDEIEKAIKN

Sequences:

>Translated_417_residues
MLTKAPRGTKDVLPSESYKWQYVENLMREICEFYGYKEIRTPGFEHTELFLRGVGESTDIVRKEMYTFNDRSGRSITLKA
EGTSPAVRAFIEHGLYAETQPTKLYYITPVYRYEKPQAGRLREHHQFGVEIFGAKSASADAEVISIAMTLLKKLGLNNLE
LRINSVGCPVCRKNYNKVLKEFLKEHLDELCDDCKVRYEVNPLRVLDCKVESCRRVTGEAPLITDYLCDDCRNHFEELKK
YLDAMGYDYIVDPRIVRGLDYYTKTAFEIISKDIGAQGTVCGGGRYDGLIEECGGPSMPGVGFGMGIERLLLTLEQNGIE
IPKPEGPDLFIAYIGDEAKLFTFTLANKLRFNGLKVEIDHMERSLKAQMKYANKLNAKFAVVIGEEELESKKVKLKNMAT
GEETEILIDEIEKAIKN
>Mature_417_residues
MLTKAPRGTKDVLPSESYKWQYVENLMREICEFYGYKEIRTPGFEHTELFLRGVGESTDIVRKEMYTFNDRSGRSITLKA
EGTSPAVRAFIEHGLYAETQPTKLYYITPVYRYEKPQAGRLREHHQFGVEIFGAKSASADAEVISIAMTLLKKLGLNNLE
LRINSVGCPVCRKNYNKVLKEFLKEHLDELCDDCKVRYEVNPLRVLDCKVESCRRVTGEAPLITDYLCDDCRNHFEELKK
YLDAMGYDYIVDPRIVRGLDYYTKTAFEIISKDIGAQGTVCGGGRYDGLIEECGGPSMPGVGFGMGIERLLLTLEQNGIE
IPKPEGPDLFIAYIGDEAKLFTFTLANKLRFNGLKVEIDHMERSLKAQMKYANKLNAKFAVVIGEEELESKKVKLKNMAT
GEETEILIDEIEKAIKN

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family

Homologues:

Organism=Homo sapiens, GI15029520, Length=473, Percent_Identity=24.5243128964059, Blast_Score=106, Evalue=4e-23,
Organism=Homo sapiens, GI6996014, Length=469, Percent_Identity=25.7995735607676, Blast_Score=104, Evalue=2e-22,
Organism=Escherichia coli, GI1788861, Length=417, Percent_Identity=41.9664268585132, Blast_Score=350, Evalue=8e-98,
Organism=Caenorhabditis elegans, GI71993693, Length=462, Percent_Identity=25.974025974026, Blast_Score=110, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI71993686, Length=462, Percent_Identity=25.974025974026, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6325290, Length=457, Percent_Identity=23.6323851203501, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24643061, Length=468, Percent_Identity=24.7863247863248, Blast_Score=105, Evalue=4e-23,
Organism=Drosophila melanogaster, GI24643059, Length=487, Percent_Identity=23.8193018480493, Blast_Score=96, Evalue=6e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): SYH_THETN (Q8RAI8)

Other databases:

- EMBL:   AE008691
- RefSeq:   NP_622853.1
- ProteinModelPortal:   Q8RAI8
- SMR:   Q8RAI8
- GeneID:   997512
- GenomeReviews:   AE008691_GR
- KEGG:   tte:TTE1230
- NMPDR:   fig|273068.3.peg.1020
- HOGENOM:   HBG616575
- OMA:   VFEWVTT
- ProtClustDB:   PRK00037
- BioCyc:   TTEN273068:TTE1230-MONOMER
- BRENDA:   6.1.1.21
- GO:   GO:0005737
- HAMAP:   MF_00127
- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516
- Gene3D:   G3DSA:3.40.50.800
- PANTHER:   PTHR11476
- PIRSF:   PIRSF001549
- TIGRFAMs:   TIGR00442

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; SSF52954 Anticodon_bd

EC number: =6.1.1.21

Molecular weight: Translated: 47365; Mature: 47365

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTKAPRGTKDVLPSESYKWQYVENLMREICEFYGYKEIRTPGFEHTELFLRGVGESTDI
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHCCCCCHHH
VRKEMYTFNDRSGRSITLKAEGTSPAVRAFIEHGLYAETQPTKLYYITPVYRYEKPQAGR
HHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCCC
LREHHQFGVEIFGAKSASADAEVISIAMTLLKKLGLNNLELRINSVGCPVCRKNYNKVLK
HHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHH
EFLKEHLDELCDDCKVRYEVNPLRVLDCKVESCRRVTGEAPLITDYLCDDCRNHFEELKK
HHHHHHHHHHHHHCEEEEECCCEEEEECCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
YLDAMGYDYIVDPRIVRGLDYYTKTAFEIISKDIGAQGTVCGGGRYDGLIEECGGPSMPG
HHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCHHHHCCCCCCCC
VGFGMGIERLLLTLEQNGIEIPKPEGPDLFIAYIGDEAKLFTFTLANKLRFNGLKVEIDH
CCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCEEEEEEEHHHHEECCEEEEHHH
MERSLKAQMKYANKLNAKFAVVIGEEELESKKVKLKNMATGEETEILIDEIEKAIKN
HHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure
MLTKAPRGTKDVLPSESYKWQYVENLMREICEFYGYKEIRTPGFEHTELFLRGVGESTDI
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHCCCCCCHHHHHHHCCCCCHHH
VRKEMYTFNDRSGRSITLKAEGTSPAVRAFIEHGLYAETQPTKLYYITPVYRYEKPQAGR
HHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHCCCCCCCCCCEEEEEEEEEECCCCCCCC
LREHHQFGVEIFGAKSASADAEVISIAMTLLKKLGLNNLELRINSVGCPVCRKNYNKVLK
HHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHHHH
EFLKEHLDELCDDCKVRYEVNPLRVLDCKVESCRRVTGEAPLITDYLCDDCRNHFEELKK
HHHHHHHHHHHHHCEEEEECCCEEEEECCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
YLDAMGYDYIVDPRIVRGLDYYTKTAFEIISKDIGAQGTVCGGGRYDGLIEECGGPSMPG
HHHHCCCCEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCHHHHCCCCCCCC
VGFGMGIERLLLTLEQNGIEIPKPEGPDLFIAYIGDEAKLFTFTLANKLRFNGLKVEIDH
CCHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCEEEEEEEHHHHEECCEEEEHHH
MERSLKAQMKYANKLNAKFAVVIGEEELESKKVKLKNMATGEETEILIDEIEKAIKN
HHHHHHHHHHHHHHCCCEEEEEECHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336